5-HT1D Antibody

Product Discontinued

5-HT1D Antibody Summary

• Immunogen: This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 1-18 and 251-267 of rat 5-HT1DR (Genbank accession no. NP_036984).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTR1D
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:10
  • Western Blot 2 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for 5-HT1D Antibody

• 5HT1D
• 5-HT1D
• 5-HT-1D
• 5-HT-1D-alpha
• 5-HT1DHTR1DA
• 5-hydroxytryptamine (serotonin) receptor 1D
• HT1DA
• HTR1D
• HTR1DA
• HTRL
• RDC4
• RDC4,5-hydroxytryptamine receptor 1D
• Serotonin receptor 1D

Background

Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. The 5-HT1D (formerly known as 5-HT1Da) receptor has 63% overall structural homology with the 5-HT1B receptor and a 77% amino acid sequence homology in the seven transmembrane domains. In situ hybridization studies have detected highest level of mRNA in primary olfactory cortex, accumbens nucleus, caudate putamen, lateral mammillary nucleus and medial vestibular nucleus. The presence of relatively high levels of 5-HT1D mRNA in the locus coeruleus suggest that, when expressed on nerve terminals, this receptor could modulate the release of catecholamines. The 5-HT1D receptor may function as an auto receptor. Like 5-HT1B receptors, 5-HT1D receptors also appear to act as heteroreceptors. Acting on the 5-HT1D receptors, 5-HT appears to inhibit release of glutamate from rat cerebellar synaptosomes and acetylcholine from guinea pig hippocampal synaptosomes. It has been suggested that neurogenic inflammation and nociceptive activity within trigeminovascular afferents. Endothelium-dependent relaxation in the pig coronary artery has been claimed to be mediated by 5-HT1D receptors. Like other members of the 5-HT1 family, 5-HT1D receptors are negatively coupled to adenylate cyclase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT1D Antibody (NB100-56349)(3)

Publications using NB100-56349

• Gautam J, Banskota S, Regmi SC et al. Tryptophan hydroxylase 1 and 5-HT7 receptor preferentially expressed in triple-negative breast cancer promote cancer progression through autocrine serotonin signaling. Mol. Cancer. 2016-11-21 [PMID: 27871326] (WB, Human)
• Harriott AM, Gold MS. Serotonin type 1D receptors (5HTR) are differentially distributed in nerve fibres innervating craniofacial tissues. Cephalalgia. 2008-09-01 [PMID: 18557979] (WB, Rat)
  
  Details:
  Antibody cited: Serotinin Receptor 5-HT1D (IMG-365). WB: various migraine associate tissues, IHC-FrFl: various nerve tissue ; Rat. (FIGs 1,2,6)
• Xiao M, Zhang Y, Feng W et al. Early growth response 2 regulates mouse cooperative behavior Research Square 2020-12-02 (WB, Mouse)

Bioinformatics

• Gene Symbol: HTR1D
• Entrez:
  • 15553(Mouse)
  • 25323(Rat)
• Uniprot:
  • P28565(Rat)