Human 4-1BB/TNFRSF9/CD137 ELISA Kit (Colorimetric)

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4-1BB/TNFRSF9/CD137 ELISA Kit [NBP1-91278] - Data for Human sCD137 (s4-1BB) Platinum ELISA

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.2 ng/mL

Order Details

Human 4-1BB/TNFRSF9/CD137 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a human 4-1BB/TNFRSF9/CD137 positive serum. There was no crossreactivity detected.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.2 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 9.90%
Intra-Assay
CV% < 7.10%
Spike Recovery
90%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFRSF9

Applications/Dilutions

Application Notes
The human sCD137 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD137.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Human sCD137 Standard lyophilized, 100 ng/ml upon reconstitution
  2. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  3. Biotin-Conjugate anti-human sCD137 polyclonal antibody
  4. Adhesive Films
  5. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD137
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human 4-1BB/TNFRSF9/CD137 ELISA Kit (Colorimetric)

  • 4-1BB ligand receptor
  • 41BB
  • 4-1BB
  • CD137 antigen
  • CD137
  • CD137MGC2172
  • CDw137
  • FLJ43501
  • ILA
  • ILAhomolog of mouse 4-1BB
  • induced by lymphocyte activation (ILA)
  • interleukin-activated receptor, homolog of mouse Ly63
  • receptor protein 4-1BB
  • T cell antigen ILA
  • T-cell antigen 4-1BB homolog
  • T-cell antigen ILA
  • TNFRSF9
  • tumor necrosis factor receptor superfamily member 9
  • tumor necrosis factor receptor superfamily, member 9

Background

The human sCD137 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD137. CD137 (ILA/4-1BB) is a member of the tumor necrosis factor receptor family, expressed on activated T lymphocytes. The expression of CD137 is strictly activation dependent in primary cells. Co-stimulation through CD137 enhances T lymphocyte activation and may enable rejection of tumors in vivo. The gene for human CD137 resides on chromosome 1q36, in a cluster of related genes, and this chromosomal region is associated with mutations in several malignancies. Soluble forms of members of the TNF receptor family have been demonstrated for the various members. The soluble isoforms of CD137, sCD137, are generated by differential splicing and can be detected at enhanced concentrations in sera of patients suffering from various diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TNFRSF9