15-PGDH/HPGD Antibody

Western Blot: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of paraffin embedded Human Liver. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

15-PGDH/HPGD Antibody Summary

• Immunogen: Peptide with sequence C-DYDTTPFQAKTQ corresponding to C-Terminus according to NP_000851.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HPGD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 1 ug/mL
• Application Notes: WB: Experiments gave bands at approx. 26 kDa band and 50 kDa band in human duodenum lysates after antibody staining. These bands correspond to earlier findings in scientific literature with different antibodies (PMID: 11889207). This protein has a calculated MW of 29.0 kDa band according to NP_000851.
• Control:

  15-PGDH/HPGD Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 15-PGDH/HPGD Antibody

• 15-hydroxyprostaglandin dehydrogenase [NAD+]
• 15PGDH
• 15-PGDH
• EC 1.1.1
• EC 1.1.1.141
• HPGD
• hydroxyprostaglandin dehydrogenase 15-(NAD)
• NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
• PGDH1
• PGDH1PGDH
• Prostaglandin dehydrogenase 1
• SDR36C1
• short chain dehydrogenase/reductase family 36C, member 1

Background

Prostaglandins are involved in many physiologic and cellular processes, such as inflammation. The NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH, type I) is the main enzyme of prostaglandin degradation. By catalyzing the conversion of the 15-hydroxyl group of prostaglandins into a keto group, this ubiquitous enzyme strongly reduces the biologic activity of these molecules. The induction of 15-PGDH expression by dexamethasone and other glucocorticoids at the therapeutic level provides an additional biochemical mechanism for the anti-inflammatory action of these glucocorticoids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 15-PGDH/HPGD Antibody (NB100-1482)(2)

Publications using NB100-1482

• Giannoulias D, Patel FA, Holloway AC et al. Differential changes in 15-hydroxyprostaglandin dehydrogenase and prostaglandin H synthase (types I and II) in human pregnant myometrium. J Clin Endocrinol Metab 2002-03-01 [PMID: 11889207]
• Coggins KG, Latour A, Nguyen MS et al. Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus. Nat Med 2002-02-01 [PMID: 11821873]

Bioinformatics

• Gene Symbol: HPGD
• Entrez:
  • 3248(Human)
• Uniprot:
  • P15428(Human)