14-3-3 gamma [ac Val2] Antibody (KC21)

Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - HeLa (lane 1), PC12 (lane 2) and NIH-3T3 (lane 3) whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.

Immunocytochemistry/Immunofluorescence: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).

Simple Western: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clone: KC21
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

14-3-3 gamma [ac Val2] Antibody (KC21) Summary

• Immunogen: N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated [UniProt# P61981]
• Modification: ac Val2
• Localization: Cytoplasmic
• Specificity: This antibody is specific for the naturally occurring human form of 14-3-3 gamma, where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: YWHAG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:3000
  • Simple Western 1:12.5
  • Immunocytochemistry/Immunofluorescence 1:500
• Application Notes: This 14-3-3 gamma [ac Val2] (KC21) antibody can be used in Immunocytochemistry/Immunofluorescence and Western Blot where a distinct band around 33 kDa is detected. In ICC/IF membrane staining was observed.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  HeLa Lysate (NB800-PC1)
  14-3-3 gamma Lysate (NBL1-17949)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for 14-3-3 gamma [ac Val2] Antibody (KC21)

• 1433 gamma
• 14-3-3 gamma
• 14-3-3 protein gamma
• 14-3-3GAMMA
• KCIP-1
• Protein kinase C inhibitor protein 1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide
• YWHAG

Background

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAG
• Entrez:
  • 7532(Human)
  • 22628(Mouse)
  • 56010(Rat)
• Uniprot:
  • P61981()