14-3-3 gamma [ac Val2] Antibody (KC21)

Images

 
Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - HeLa (lane 1), PC12 (lane 2) and NIH-3T3 (lane 3) whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The ...read more
Immunocytochemistry/ Immunofluorescence: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were ...read more
Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).
Simple Western: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
KC21
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

14-3-3 gamma [ac Val2] Antibody (KC21) Summary

Immunogen
N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated [UniProt# P61981]
Modification
ac Val2
Localization
Cytoplasmic
Specificity
This antibody is specific for the naturally occurring human form of 14-3-3 gamma, where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
YWHAG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • Simple Western 1:12.5
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This 14-3-3 gamma [ac Val2] (KC21) antibody can be used in Immunocytochemistry/Immunofluorescence and Western Blot where a distinct band around 33 kDa is detected. In ICC/IF membrane staining was observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
14-3-3 gamma Lysate (NBL1-17949)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for 14-3-3 gamma [ac Val2] Antibody (KC21)

  • 1433 gamma
  • 14-3-3 gamma
  • 14-3-3 protein gamma
  • 14-3-3GAMMA
  • KCIP-1
  • Protein kinase C inhibitor protein 1
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide
  • YWHAG

Background

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-27202
Species: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Op, Other, Pm
Applications: WB, Simple Western
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
MAB2669
Species: Hu
Applications: WB, Simple Western, IHC
NB100-464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
NBP1-89827
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IF
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-46403
Species: Hu
Applications: IHC
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-46404
Species: Hu
Applications: IHC, IHC-P
NBP1-84168
Species: Hu
Applications: IHC, IHC-P
NBP2-42388
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-33687
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-406
Species: Hu
Applications: WB, Simple Western, ICC/IF

Publications for 14-3-3 gamma Antibody (NB100-406) (0)

There are no publications for 14-3-3 gamma Antibody (NB100-406).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 14-3-3 gamma Antibody (NB100-406) (0)

There are no reviews for 14-3-3 gamma Antibody (NB100-406). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 14-3-3 gamma Antibody (NB100-406) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional 14-3-3 gamma Products

Bioinformatics Tool for 14-3-3 gamma Antibody (NB100-406)

Discover related pathways, diseases and genes to 14-3-3 gamma Antibody (NB100-406). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 14-3-3 gamma Antibody (NB100-406)

Discover more about diseases related to 14-3-3 gamma Antibody (NB100-406).
 

Pathways for 14-3-3 gamma Antibody (NB100-406)

View related products by pathway.

PTMs for 14-3-3 gamma Antibody (NB100-406)

Learn more about PTMs related to 14-3-3 gamma Antibody (NB100-406).
 

Research Areas for 14-3-3 gamma Antibody (NB100-406)

Find related products by research area.

Blogs on 14-3-3 gamma

There are no specific blogs for 14-3-3 gamma, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our 14-3-3 gamma [ac Val2] Antibody (KC21) and receive a gift card or discount.

Bioinformatics

Gene Symbol YWHAG
Entrez
Uniprot