14-3-3 gamma [ac Val2] Antibody (KC21)

Images

 
Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed ...read more
Immunocytochemistry/ Immunofluorescence: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were ...read more
Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).
Simple Western: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - Lane view shows a specific band for 14-3-3 Gamma in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
KC21
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

14-3-3 gamma [ac Val2] Antibody (KC21) Summary

Immunogen
N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated [UniProt# P61981]
Modification
ac Val2
Localization
Cytoplasmic
Specificity
This antibody is specific for the naturally occurring human form of 14-3-3 gamma, where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
YWHAG
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • Simple Western 1:12.5
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This 14-3-3 gamma [ac Val2] (KC21) antibody can be used in Immunocytochemistry/Immunofluorescence and Western Blot where a distinct band around 33 kDa is detected. In ICC/IF membrane staining was observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
14-3-3 gamma Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for 14-3-3 gamma [ac Val2] Antibody (KC21)

  • 1433 gamma
  • 14-3-3 gamma
  • 14-3-3 protein gamma
  • 14-3-3GAMMA
  • KCIP-1
  • Protein kinase C inhibitor protein 1
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide
  • YWHAG

Background

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for 14-3-3 gamma Antibody (NB100-406): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 14-3-3 gamma Antibody (NB100-406) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-406AF350
Alexa Fluor 405 NB100-406AF405
Alexa Fluor 488 NB100-406AF488
Alexa Fluor 532 NB100-406AF532
Alexa Fluor 594 NB100-406AF594
Alexa Fluor 647 NB100-406AF647
Alexa Fluor 700 NB100-406AF700
Alexa Fluor 750 NB100-406AF750
Biotin NB100-406B
DyLight 350 NB100-406UV
DyLight 405 NB100-406V
DyLight 488 NB100-406G
DyLight 550 NB100-406R
DyLight 594 NB100-406DL594
DyLight 650 NB100-406C
DyLight 680 NB100-406FR
DyLight 755 NB100-406IR
FITC NB100-406F
HRP NB100-406H
Janelia Fluor 549 NB100-406JF549
Janelia Fluor 646 NB100-406JF646

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Bioinformatics

Gene Symbol YWHAG
Entrez
Uniprot