14-3-3 beta/alpha Antibody

Images

 
Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2]. HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.
Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 E: Molt-4 F: Raji 12% SDS PAGE diluted at 1:10000
Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.
Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 E: Molt-4 F: Raji 12% SDS PAGE; antibody diluted at 1:10000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
 

Independent Antibodies

       

Order Details

14-3-3 beta/alpha Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human 14-3-3 beta. The exact sequence is proprietary.
Localization
Cytoplasm, Melanosome
Predicted Species
Bovine (100%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YWHAB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse, Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 14-3-3 beta/alpha Antibody

  • 14-3-3 alpha
  • 14-3-3 beta
  • 14-3-3 protein beta/alpha
  • brain protein 14-3-3, beta isoform
  • GW128
  • HS1
  • KCIP-1
  • Protein 1054
  • Protein kinase C inhibitor protein 1
  • protein kinase C inhibitor protein-1
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide
  • YWHAA

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol YWHAB
Entrez