14-3-3 Antibody

Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: 14-3-3 Antibody [NBP2-27370] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Pm, RM
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

14-3-3 Antibody Summary

• Immunogen: A portion of amino acids 40-90 from human 14-3-3 was used as the immunogen for this antibody.
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAQ
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:10
  • Western Blot 2-4ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 34 kDa.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in Crab-eating Macaque.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for 14-3-3 Antibody

• 14-3-3 protein tau
• 14-3-3 protein T-cell
• 14-3-3 protein theta
• 1433
• 14-3-3
• 14-3-3,1C5
• HS1
• Protein HS1
• protein tau
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide

Background

The 14-3-3 family of proteins are important regulatory molecules ubiquitiously expressed in all eukarryotic cells which bind to numerous signaling proteins in various pathways driving critical cellular pathways of apoptosis, differentiation and cell cycle (1-3). 14-3-3 theta specifically has also been shown to play an important regulatory role in the TLR2 signaling pathways (1) as a negative regulator of TLR2 ligand Pam3CySk4 induced NF-kB activation. 14-3-3 theta has previously been shown to interact with TLR4 ligand and MyD88 dependent phosphorylated PkC epsilon (3). 14-3-3 theta in the TLR4 signaling pathway is a positive regulator controlling release of IRF3 indcuded pro-inflammatory cytokines RANTES and IP-10 (3, 4). Currently identified by mass spec as part of the TLR2 signaling complex (1) and taken along with TLR4 data, a 14-3-3 theta antibody can be used to examine the different regulatory functions of 14-3-3 theta for different TLRs through its interaction with common or unique TLR signaling adaptor molecules in addition to MyD88 or PkC epsilon such as TRAM or TRIF allowing further clarification of TLR specific pathway regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAQ
• Uniprot:
  • P27348()