14-3-3 Antibody

Images

 
Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat ...read more
Simple Western: 14-3-3 Antibody [NBP2-27370] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

14-3-3 Antibody Summary

Immunogen
A portion of amino acids 40-90 from human 14-3-3 was used as the immunogen for this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YWHAQ
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4ug/ml
  • Simple Western 1:10
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in human, crab-eating macaque, and rhesus monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for 14-3-3 Antibody

  • 14-3-3 protein tau
  • 14-3-3 protein T-cell
  • 14-3-3 protein theta
  • 1433
  • 14-3-3
  • 14-3-3,1C5
  • HS1
  • Protein HS1
  • protein tau
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide

Background

The 14-3-3 family of proteins are important regulatory molecules ubiquitiously expressed in all eukarryotic cells which bind to numerous signaling proteins in various pathways driving critical cellular pathways of apoptosis, differentiation and cell cycle (1-3). 14-3-3 theta specifically has also been shown to play an important regulatory role in the TLR2 signaling pathways (1) as a negative regulator of TLR2 ligand Pam3CySk4 induced NF-kB activation. 14-3-3 theta has previously been shown to interact with TLR4 ligand and MyD88 dependent phosphorylated PkC epsilon (3). 14-3-3 theta in the TLR4 signaling pathway is a positive regulator controlling release of IRF3 indcuded pro-inflammatory cytokines RANTES and IP-10 (3, 4). Currently identified by mass spec as part of the TLR2 signaling complex (1) and taken along with TLR4 data, a 14-3-3 theta antibody can be used to examine the different regulatory functions of 14-3-3 theta for different TLRs through its interaction with common or unique TLR signaling adaptor molecules in addition to MyD88 or PkC epsilon such as TRAM or TRIF allowing further clarification of TLR specific pathway regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, ICC, IHC-FrFl
NB100-82245
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP2-67569
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
7954-GM/CF
Species: Hu
NBP2-42388
Species: Hu
Applications: WB, IHC, IHC-P, KD
MAB4540
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF800
Species: Hu, Mu
Applications: WB, Simple Western, IP
NB100-56080
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-33687
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF5789
Species: Hu, Mu, Rt
Applications: WB
NBP2-67481
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1768
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82257
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-27370
Species: Hu, Pm
Applications: WB, Simple Western
Supplier Logo

Publications for 14-3-3 Antibody (NBP2-27370) (0)

There are no publications for 14-3-3 Antibody (NBP2-27370).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 14-3-3 Antibody (NBP2-27370) (0)

There are no reviews for 14-3-3 Antibody (NBP2-27370). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for 14-3-3 Antibody (NBP2-27370) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NBP2-27370

Bioinformatics Tool for 14-3-3 Antibody (NBP2-27370)

Discover related pathways, diseases and genes to 14-3-3 Antibody (NBP2-27370). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 14-3-3 Antibody (NBP2-27370)

Discover more about diseases related to 14-3-3 Antibody (NBP2-27370).
 

Pathways for 14-3-3 Antibody (NBP2-27370)

View related products by pathway.

Research Areas for 14-3-3 Antibody (NBP2-27370)

Find related products by research area.

Blogs on 14-3-3.

Role of RASSF1A in Death Receptor-Dependent Apoptosis
Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and t...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our 14-3-3 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol YWHAQ
Uniprot