11 beta-HSD1 Antibody

Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (60 ug protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence. Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queen's Medical Research Institute, Edinburgh, UK

Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt
• Applications: WB, ELISA, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

11 beta-HSD1 Antibody Summary

• Immunogen: Peptide with sequence C-TARSEEGLQKVVSR corresponding to internal region according to NP_032314.2.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HSD11B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 32 kDa band observed in mouse lung lysates (calculated MW of 32.4 kDa band according to NP_032314.2). An additional band of unknown identity was also consistently observed at 45 kDa. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 11 beta-HSD1 Antibody

• 11 betaHSD1
• 11 beta-HSD1
• 11-beta-hydroxysteroid dehydrogenase 1
• EC 1.1.1
• EC 1.1.1.146
• HDL
• HSD11B1
• HSD11HSD11B
• HSD11LMGC13539
• hydroxysteroid (11-beta) dehydrogenase 1
• member 1
• SDR26C1

Background

HSD11B1 is encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 11 beta-HSD1 Antibody (NBP2-27469)(1)

Publication using NBP2-27469

• Sinclair AJ, Walker EA, Burdon MA et al. Cerebrospinal fluid corticosteroid levels and cortisol metabolism in patients with idiopathic intracranial hypertension: a link between 11beta-HSD1 and intracranial pressure regulation? J Clin Endocrinol Metab 2010-12-01 [PMID: 20826586]

Bioinformatics

• Gene Symbol: HSD11B1
• Entrez:
  • 15483(Mouse)
  • 25116(Rat)
• Uniprot:
  • NP_032314.2(Human)