Product Search for cd4a: Novus Biologicals

NBP3-24701

C2CD4A Antibody - BSA Free

100 ul

NBP3-24701

Staining of human cell line OE19 shows localization to nucleoplasm.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

AF566

Neuropilin-1 Antibody [Unconjugated]

(10 Reviews)

(110 Publications)

100 μg

AF566

Western blot shows 25 ng of Recombinant Mouse Neuropilin-1 (Catalog # 5994-N1), Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin-1 (Catalog # 3870-N1). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neuropilin-1 was detected in immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.) using 5 µg/mL Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neuropilin‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the dorsal horn. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neuropilin‑1 was detected in immersion fixed paraffin-embedded sections of rat brain (hippocampus) using Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neuropilin‑1 was detected in immersion fixed paraffin-embedded sections of rat brain (thalamus) using Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Nrp1 influences Smad2/3 activation.(a–c) Representative confocal images of wt sprouts from EBs immunolabelled for pSmad2. The outline of the wt sprouts is indicated with a dashed line using the endogenous DsRED marker. DAPI staining (not shown) was used to mark nuclei (blue line). Sprouts from (a) untreated EBs, (b) treated with 2 ng ml−1 Tgf-beta for 1 h and(c) treated with 10 μM SB-421543 for 4 h. Scale bar, 13 μm. (d,e) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Fig. 10. A representative blot of six is shown; P=0.0012 NRP1 siRNA compared with control. (f,g) Proteins from P4 HUVEC transfected with control-GFP and NRP1–GFP–His construct for 24 h, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h were assessed for SMAD2 phosphorylation. Full western blots are shown in Supplementary Fig. 11. A representative blot of four is shown; P=0.0017 NRP1 overexpression compared with control. (e,g) Quantification of pSMAD2 protein normalized to SMAD2/3. (h–k) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Figs 12 and 13. (i) Quantification of pSMAD2 protein normalized to SMAD2/3; P=0.0848 Nrp1 siRNA compared with control. (k) Quantification of pSMAD3 protein normalized to SMAD3; P=0.0181 NRP1 siRNA compared with control. (l,m) Western blot analysis of P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 10 ng ml−1 BMP9 for 15 and 30 min. Full western blots are shown in Supplementary Fig. 14. A representative blot of four is shown. (m) Quantification of pSMAD2/3 protein normalized to SMAD2; P<0.0011 NRP1 siRNA compared with control. All values represent mean±s.e.m. DAPI, 4,6-diamidino-2-phenylindole; NS, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26081042), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of candidate genes. (A) qPCR analysis of relative mRNA levels in total RNA from isolated glomeruli of Foxc2fl/fl; Pod‐Cre+, Foxc2fl/+; Pod‐Cre+ and Foxc2fl/+ mice. Expression levels were normalized against the expression of Rplp0. (B) qPCR analysis of relative mRNA levels in total RNA from a podocyte cell line treated with either Foxc2 siRNA or non‐targeting siRNA. (C) Western blot analysis of NRP1 and ITGB1 protein expression after Foxc2 induction (pBABE‐Foxc2) or knockdown (Foxc2 siRNA). Empty vector (pBABE) and non‐targeting siRNA was used as controls. *P < 0.05 Foxc2fl/fl;Pod‐Cre+ versus Foxc2fl/+. Error bars in (A) and (B) represent SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31062503), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>Nrp1 influences Smad2/3 activation.(a–c) Representative confocal images of wt sprouts from EBs immunolabelled for pSmad2. The outline of the wt sprouts is indicated with a dashed line using the endogenous DsRED marker. DAPI staining (not shown) was used to mark nuclei (blue line). Sprouts from (a) untreated EBs, (b) treated with 2 ng ml−1 Tgf-beta for 1 h and(c) treated with 10 μM SB-421543 for 4 h. Scale bar, 13 μm. (d,e) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Fig. 10. A representative blot of six is shown; P=0.0012 NRP1 siRNA compared with control. (f,g) Proteins from P4 HUVEC transfected with control-GFP and NRP1–GFP–His construct for 24 h, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h were assessed for SMAD2 phosphorylation. Full western blots are shown in Supplementary Fig. 11. A representative blot of four is shown; P=0.0017 NRP1 overexpression compared with control. (e,g) Quantification of pSMAD2 protein normalized to SMAD2/3. (h–k) Western blot analysis of proteins from P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 2 ng ml−1 TGF-beta for 1 h. Full western blots are shown in Supplementary Figs 12 and 13. (i) Quantification of pSMAD2 protein normalized to SMAD2/3; P=0.0848 Nrp1 siRNA compared with control. (k) Quantification of pSMAD3 protein normalized to SMAD3; P=0.0181 NRP1 siRNA compared with control. (l,m) Western blot analysis of P4 HUVEC transfected with control siRNA and NRP1 siRNA, with or without stimulation with 10 ng ml−1 BMP9 for 15 and 30 min. Full western blots are shown in Supplementary Fig. 14. A representative blot of four is shown. (m) Quantification of pSMAD2/3 protein normalized to SMAD2; P<0.0011 NRP1 siRNA compared with control. All values represent mean±s.e.m. DAPI, 4,6-diamidino-2-phenylindole; NS, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26081042), licensed under a CC-BY license. Not internally tested by R&D Systems.

Rescue of VEGF-A Signaling in Nrp1cyto and Nrp1fl/fl Primary EC by Full-Length NRP1 and of EC Tubulogenesis following NRP1 siRNA Knockdown with Constitutively Active ERK(A and B) Western blot analysis of Nrp1cyto EC that were transduced with the indicated adenoviral constructs, serum-starved and stimulated with 50 ng/ml VEGF-A165. Blots (A) and quantitation of ERK activation (B) show that Ad-Nrp1, but not Ad-Nrp1cyto or Ad-Nrp1PDZ, restores VEGF-A-induced VEGFR2 and ERK activation in Nrp1cyto EC (mean ± SD, n = 3, *p < 0.05).(C and D) Western blot analysis of Nrp1fl/fl EC treated with adenoviral constructs expressing CRE recombinase to inactivate NRP1 were transduced with the indicated adenoviral constructs, serum-starved and stimulated with 50 ng/ml VEGF-A165. Blots (C) and quantitation (D) show that Ad-Nrp1, but not Ad-Nrp1cyto or Ad-Nrp1PDZ, restores the activation of VEGFR2 and ERK (mean ± SD, n = 3, *p < 0.05).(E and F) HUVEC treated with control or NRP1 siRNA were treated with the indicated adenoviral vectors to compare their capacity to undergo tube formation in 3D collagen matrices after 72 hr. Representative images (E) and quantification (F) of tubulogenesis after 72 hr (mean ± SEM, n = 18, *p < 0.05). Scale bar represents 100 um. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.

Impaired VEGF-A Signaling in Nrp1cyto Mice(A) Western blot analysis of heart lysates following intraperitoneal injection of VEGF-A. Blots show reduced ERK and VEGFR2 phosphorylation in Nrp1cyto mice relative to WT littermates.(B–D) Western blot analysis of cell lysates from primary arterial EC from Nrp1cyto and WT EC that were serum-starved and then stimulated for the indicated times with 50 ng/ml VEGF-A165. (B) Blots show reduced phosphorylation of VEGFR2 on Y1175 and of ERK in Nrp1cyto relative to control EC. (C) Quantification of the ratio of phospho-VEGFR2 (pVEGFR2) relative to total VEGFR2 (VEGFR2) (mean ± SD, n = 5, *p < 0.05). (D) Quantification of the ratio of phospho-ERK (pERK) relative to total ERK (ERK) (mean ± SD, n = 5, *p < 0.05).(E) Western blot analysis of cell lysates from Nrp1cyto and WT primary arterial EC that were serum-starved and stimulated for the indicated times with 50 ng/ml of FGF2 or IGF1. ERK phosphorylation was similar in Nrp1cyto and WT EC.See also Figure S4. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.

Rescue of Defective ERK Signaling in VEGF-A-Stimulated Nrp1cyto Arterial EC by Knockdown of PTP1bPrimary arterial EC from Nrp1cyto mice transfected with siRNA specific for the indicated phosphatases were serum-starved and then stimulated with 50 ng/ml VEGF-A165.(A and B) Knockdown of the indicated phosphatases in Nrp1cyto arterial EC shown by immunoblotting (A); PTP1b knockdown was quantified in (B), dashed line indicates normal expression levels.(C and D) ERK and VEGFR2 (Y1175) phosphorylation after knockdown of the indicated phosphatases shown by immunoblotting (C). Quantification of pERK activation is shown in (D) (n = 3, mean ± SD, *p < 0.05). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23639442), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF566U-100UG
Alexa Fluor 405	AF566V-100UG
Alexa Fluor 488	AF566G-100UG
Alexa Fluor 532	AF566X-100UG
Alexa Fluor 594	AF566T-100UG
Alexa Fluor 647	AF566R-100UG
Alexa Fluor 700	FAB566N
Alexa Fluor 750	AF566S-100UG
Allophycocyanin	FAB566A
Biotin	BAF566
CoraFluor 1	AF566CL1
DyLight 350	AF566D
DyLight 405	AF566E
DyLight 488	AF566K
DyLight 550	AF566L
DyLight 594	AF566M
DyLight 650	AF566W
DyLight 680	AF566Y
DyLight 755	AF566Z
Fluorescein	FAB566F
HRP	AF566H
Janelia Fluor 525	AF566JF525
Janelia Fluor 549	AF566I
Janelia Fluor 646	AF566J
Janelia Fluor 669	AF566JF669
Phycoerythrin	FAB566P
mFluor Violet 450 SE	AF566MFV450
mFluor Violet 500 SE	AF566MFV500
mFluor Violet 610 SE	AF566MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB566F
Carrier Free	FAB566P
Carrier Free	FAB566A
Carrier Free	FAB566N
Carrier Free	AF566-SP

compare

(select up to 3 products from this page to compare)

NBP2-25199

CD4 Antibody (RPA-T4) - Azide and BSA Free

(1 Review)

(13 Publications)

0.1 mg

NBP2-25199

Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the FITC conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.

Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the DyLight 405 conjugate of NBP2-27216. Staining of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.

Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of this antibody. Green represents isotype control; red represents anti-CD4 antibody. Image using the Azide Free format of this antibody.

Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Allophycocyanin conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.

Flow Cytometry: CD4 Antibody (RPA-T4) - Azide and BSA Free [NBP2-25199] - analysis of CD4 in human PBMCs using 0.1 ug of this antibody. Secondary antibody is goat anti-mouse PE.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-5916A

Alt Catalog# IMG-5916A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-27216AF350
Alexa Fluor 405	NBP2-27216AF405
Alexa Fluor 488	NBP2-27216AF488
Alexa Fluor 532	NBP2-27216AF532
Alexa Fluor 594	NBP2-27216AF594
Alexa Fluor 647	NBP2-27216AF647
Alexa Fluor 700	NBP2-27216AF700
Alexa Fluor 750	NBP2-27216AF750
Allophycocyanin	NBP2-27245
Biotin	NBP2-25199B
DyLight 350	NBP2-27216UV
DyLight 405	NBP2-27216V
DyLight 488	NBP2-27216G
DyLight 550	NBP2-27216R
DyLight 650	NBP2-27216C
DyLight 680	NBP2-27216FR
DyLight 755	NBP2-27216IR
FITC	NBP2-27216F
HRP	NBP2-27216H
Janelia Fluor 549	NBP2-27216JF549
Janelia Fluor 646	NBP2-27216JF646
PE	NBP2-27248
PerCP	NBP2-27216PCP
mFluor Violet 450 SE	NBP2-27216MFV450
mFluor Violet 500 SE	NBP2-27216MFV500
mFluor Violet 610 SE	NBP2-27216MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-27216
Azide and BSA Free	NBP2-25199SS
Azide and BSA Free	NBP2-27216-25ug

compare

(select up to 3 products from this page to compare)

NB100-56722

TLR2 Antibody (TL2.1) - BSA Free

(64 Publications)

0.1 mg

NB100-56722

Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Total protein from human THP-1 and U937 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TLR2 (NB100-56722) in 5% BSA-TBST and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).

Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Monitoring of TLR2 expression in normal B cells and primary MCL cells cultured for 24h in the absence/presence of CD40L, IL-4, and TLR ligands. Filled curves refer to isotypic control, empty curves to the TLR2 antibody (NB100-56722) of untreated samples. Dotted histograms represent CD40L+IL4 activated cells, dashed curves indicate CD40L+IL4 activated cells further treated with TLR ligands. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0153823) licensed under a CC-BY license.

Flow (Cell Surface): TLR2 Antibody (TL2.1) [NB100-56722] - Flow (Cell Surface): - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on stable transfected cell line using this antibody at 1 ug/10^6 cells. Green: isotype control. Red: anti-TLR2 antibody. Image using the Azide Free form of this antibody.

Block/Neutralize: TLR2 Antibody (TL2.1) [NB100-56722] - Human monocyte-derived DCs were pre-incubated wit TLR-neutralizing antibodies including TLR2 antibody (NB100-56722) for 1 h. Afterwards, DCs were infected with H. pylori G27 (MOI 5) and the levels of the costimulatory molecule CD86 and the maturation marker CD83 were analyzed by FACS on CD11c+ cells 24 h post-infection. Data are presented as mean +/- SD of five independent experiments. *p<=0.05, **p<=0.005, ***p<=0.0005. Asterisks on top of bars indicate significance relative to non-neutralized, H. pylori-primed control cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104804) licensed under a CC-BY license.

Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722] - Intracellular flow analysis of TLR2 in 1x10^6 PBMCs using 2 ug of NB100-56722. Shaded histogram represents cells without antibody; green represents isotype control; purple represents anti-TLR2 antibody.

Reactivity:

Hu, Mu, Rt, Ca, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Rb

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-416A

Alt Catalog# IMG-416A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56722AF350
Alexa Fluor 405	NB100-56722AF405
Alexa Fluor 488	NB100-56722AF488
Alexa Fluor 532	NB100-56722AF532
Alexa Fluor 594	NB100-56722AF594
Alexa Fluor 647	NB100-56722AF647
Alexa Fluor 700	NB100-56722AF700
Alexa Fluor 750	NB100-56722AF750
Allophycocyanin	NB100-56722APC
Biotin	NB100-56722B
CoraFluor 1	NB100-56722CL1
DyLight 594	NB100-56722DL594
FITC	NB100-56722F
Janelia Fluor 525	NB100-56722JF525
Janelia Fluor 549	NB100-56722JF549
Janelia Fluor 646	NB100-56722JF646
Janelia Fluor 669	NB100-56722JF669
PE	NBP2-24909
PE	NB100-56722PE
mFluor Violet 450 SE	NB100-56722MFV450
mFluor Violet 500 SE	NB100-56722MFV500
mFluor Violet 610 SE	NB100-56722MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB100-56726
BSA Free	NB100-56722SS
Azide and BSA Free	NB100-56726SS

compare

(select up to 3 products from this page to compare)

NBP2-25191

CD4 Antibody (GK1.5) - BSA Free

(7 Publications)

0.1 mg

NBP2-25191

Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the Allophycocyanin conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents mouse CD4 this antibody. Image using the Azide Free format of this antibody.

Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the FITC conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of NBP2-26584. Green represents isotype control (20212C); red represents mouse CD4 NBP2-26584 antibody. Image using the Azide Free format of this antibody.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Alt Catalog#

IMG-5922A

Alt Catalog# IMG-5922A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25191AF350
Alexa Fluor 405	NBP2-25191AF405
Alexa Fluor 488	NBP2-25191AF488
Alexa Fluor 532	NBP2-25191AF532
Alexa Fluor 594	NBP2-25191AF594
Alexa Fluor 647	NBP2-25191AF647
Alexa Fluor 700	NBP2-25191AF700
Alexa Fluor 750	NBP2-25191AF750
Allophycocyanin	NBP2-26598
Allophycocyanin/Cy7	NBP2-25191APCCY7
Biotin	NBP2-25191B
CoraFluor 1	NBP2-25191CL1
DyLight 350	NBP2-25191UV
DyLight 405	NBP2-25191V
DyLight 488	NBP2-25191G
DyLight 550	NBP2-25191R
DyLight 594	NBP2-25191DL594
DyLight 650	NBP2-25191C
DyLight 680	NBP2-25191FR
DyLight 755	NBP2-25191IR
FITC	NBP2-25191F
HRP	NBP2-25191H
Janelia Fluor 525	NBP2-25191JF525
Janelia Fluor 549	NBP2-25191JF549
Janelia Fluor 646	NBP2-25191JF646
Janelia Fluor 669	NBP2-25191JF669
PE	NBP2-25191PE
PE/Atto594	NBP2-25191PEATT594
PE/Cy5.5	NBP2-25191PECY55
PE/Cy7	NBP2-25191PECY7
PerCP	NBP2-25191PCP
mFluor Violet 450 SE	NBP2-25191MFV450
mFluor Violet 500 SE	NBP2-25191MFV500
mFluor Violet 610 SE	NBP2-25191MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-26585
BSA Free	NBP2-25191SS
Azide and BSA Free	NBP2-26585-1mg
Azide and BSA Free	NBP2-26585-25ug

compare

(select up to 3 products from this page to compare)

NB100-56457

CD4 Antibody - BSA Free

(1 Publication)

0.1 mg

NB100-56457

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5808A

Alt Catalog# IMG-5808A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56457SS

compare

(select up to 3 products from this page to compare)

MAB7797

MS4A4A Antibody (818112)

100 μg

MAB7797

HEK293 human embryonic kidney cell line co-transfected with human MS4A4A and eGFP was stained with either (A) Mouse IgG2B Isotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human MS4A4A Monoclonal Antibody (Catalog # MAB7797) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7797U-100UG
Alexa Fluor 405	FAB7797V-100UG
Alexa Fluor 488	FAB7797G-100UG
Alexa Fluor 532	FAB7797X
Alexa Fluor 594	FAB7797T-100UG
Alexa Fluor 647	FAB7797R-100UG
Alexa Fluor 700	FAB7797N-100UG
Alexa Fluor 750	FAB7797S-100UG
Allophycocyanin	FAB7797A
Allophycocyanin/Cy7	FAB7797APCCY7
Biotin	FAB7797B
CoraFluor 1	FAB7797CL1
DyLight 350	FAB7797D
DyLight 405	FAB7797E
DyLight 488	FAB7797K
DyLight 550	FAB7797L
DyLight 594	FAB7797M
DyLight 650	FAB7797W
DyLight 680	FAB7797Y
DyLight 755	FAB7797Z
FITC	FAB7797F
HRP	FAB7797H
Janelia Fluor 525	FAB7797JF525
Janelia Fluor 549	FAB7797I
Janelia Fluor 646	FAB7797J
Janelia Fluor 669	FAB7797JF669
PE/Atto594	FAB7797PEATT594
PE/Cy5.5	FAB7797PECY55
PE/Cy7	FAB7797PECY7
PerCP	FAB7797C
Phycoerythrin	FAB7797P
mFluor Violet 450 SE	FAB7797MFV450
mFluor Violet 500 SE	FAB7797MFV500
mFluor Violet 610 SE	FAB7797MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB7797P
Carrier Free	MAB7797-SP
Carrier Free	FAB7797R-100UG
Carrier Free	FAB7797N-100UG
Carrier Free	FAB7797G-100UG
Carrier Free	FAB7797V-100UG
Carrier Free	FAB7797U-100UG
Carrier Free	FAB7797T-100UG
Carrier Free	FAB7797S-100UG

compare

(select up to 3 products from this page to compare)

NBP1-86542

MS4A4A Antibody - BSA Free

0.1 ml

NBP1-86542

Immunohistochemistry-Paraffin: MS4A4A Antibody [NBP1-86542] - Staining of human placenta shows membranous positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: MS4A4A Antibody [NBP1-86542] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-86542-25ul

compare

(select up to 3 products from this page to compare)

NBP2-25196

CD19 Antibody (CB19) - BSA Free

(1 Review)

(12 Publications)

0.1 mg

NBP2-25196

Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the FITC conjugate of NBP2-25196. Staining of CD19 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD19 antibody.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the PE conjugate of NBP2-25196. Staining of CD19 in 1x10^6 human PBMC using 10 ul (0.25 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25196. Staining of human PBMC. Image from verified customer review.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the DyLight 405 conjugate of NBP2-25196. Staining of CD19 in human PBMCs using anti-CD19 antibody. Image from verified customer review.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with TACI/TNFRSF13B/CVID (1A1) antibody NB600-1189PE and a matched isotype control. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6321A

Alt Catalog# IMG-6321A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25196AF350
Alexa Fluor 405	NBP2-25196AF405
Alexa Fluor 488	NBP2-25196AF488
Alexa Fluor 532	NBP2-25196AF532
Alexa Fluor 594	NBP2-25196AF594
Alexa Fluor 647	NBP2-25196AF647
Alexa Fluor 700	NBP2-25196AF700
Alexa Fluor 750	NBP2-25196AF750
Allophycocyanin	NBP2-25196APC
Allophycocyanin/Cy7	NBP2-25196APCCY7
Biotin	NBP2-25196B
CoraFluor 1	NBP2-25196CL1
DyLight 350	NBP2-25196UV
DyLight 405	NBP2-25196V
DyLight 488	NBP2-25196G
DyLight 550	NBP2-25196R
DyLight 594	NBP2-25196DL594
DyLight 650	NBP2-25196C
DyLight 680	NBP2-25196FR
DyLight 755	NBP2-25196IR
FITC	NBP2-25196F
HRP	NBP2-25196H
Janelia Fluor 525	NBP2-25196JF525
Janelia Fluor 549	NBP2-25196JF549
Janelia Fluor 646	NBP2-25196JF646
Janelia Fluor 669	NBP2-25196JF669
PE	NBP2-26646
PE/Atto594	NBP2-25196PEATT594
PE/Cy5.5	NBP2-25196PECY55
PE/Cy7	NBP2-25196PECY7
PerCP	NBP2-25196PCP
mFluor Violet 450 SE	NBP2-25196MFV450
mFluor Violet 500 SE	NBP2-25196MFV500
mFluor Violet 610 SE	NBP2-25196MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-25196SS
Azide and BSA Free	NBP2-80634

compare

(select up to 3 products from this page to compare)

MAB6247

MS4A3 Antibody (489433)

100 μg

MAB6247

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6247U-100UG
Alexa Fluor 405	FAB6247V-100UG
Alexa Fluor 488	FAB6247G-100UG
Alexa Fluor 532	FAB6247X
Alexa Fluor 594	FAB6247T-100UG
Alexa Fluor 647	FAB6247R-100UG
Alexa Fluor 700	FAB6247N-100UG
Alexa Fluor 750	FAB6247S-100UG
Allophycocyanin	FAB6247A
Allophycocyanin/Cy7	FAB6247APCCY7
Biotin	FAB6247B
CoraFluor 1	FAB6247CL1
DyLight 350	FAB6247D
DyLight 405	FAB6247E
DyLight 488	FAB6247K
DyLight 550	FAB6247L
DyLight 594	FAB6247M
DyLight 650	FAB6247W
DyLight 680	FAB6247Y
DyLight 755	FAB6247Z
Fluorescein	FAB6247F
HRP	FAB6247H
Janelia Fluor 525	FAB6247JF525
Janelia Fluor 549	FAB6247I
Janelia Fluor 646	FAB6247J
Janelia Fluor 669	FAB6247JF669
PE/Atto594	FAB6247PEATT594
PE/Cy5.5	FAB6247PECY55
PE/Cy7	FAB6247PECY7
PerCP	FAB6247C
mFluor Violet 450 SE	FAB6247MFV450
mFluor Violet 500 SE	FAB6247MFV500
mFluor Violet 610 SE	FAB6247MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB6247F
Carrier Free	MAB6247-SP
Carrier Free	FAB6247V-100UG
Carrier Free	FAB6247U-100UG
Carrier Free	FAB6247T-100UG
Carrier Free	FAB6247S-100UG
Carrier Free	FAB6247R-100UG
Carrier Free	FAB6247N-100UG
Carrier Free	FAB6247G-100UG

compare

(select up to 3 products from this page to compare)

NB100-56560

TLR4 Antibody (MTS510)

(1 Review)

(14 Publications)

0.1 mg

NB100-56560

Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram: Cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.

Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of cell surface TLR4 using this antibody at 0.625 ug/10^6 Raw cells (pretreated with anti-CD16/32). Shaded histogram: isotype control.

Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.

Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in RAW cells using 0.5 ug of this antibody. Black histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Alt Catalog#

IMG-428A

Alt Catalog# IMG-428A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24865AF350
Alexa Fluor 405	NBP2-24865AF405
Alexa Fluor 488	NBP2-24865AF488
Alexa Fluor 532	NBP2-24865AF532
Alexa Fluor 594	NBP2-24865AF594
Alexa Fluor 647	NBP2-24865AF647
Alexa Fluor 700	NBP2-24865AF700
Alexa Fluor 750	NBP2-24865AF750
DyLight 350	NBP2-24865UV
DyLight 405	NBP2-24865V
DyLight 488	NBP2-24865G
DyLight 550	NBP2-24865R
DyLight 594	NB100-56560DL594
DyLight 650	NBP2-24865C
DyLight 680	NBP2-24865FR
DyLight 755	NBP2-24865IR
FITC	NBP2-24450
Janelia Fluor 549	NBP2-24865JF549
Janelia Fluor 646	NBP2-24865JF646
PE	NBP2-24741
PerCP	NBP2-24865PCP
mFluor Violet 450 SE	NBP2-24865MFV450
mFluor Violet 500 SE	NBP2-24865MFV500
mFluor Violet 610 SE	NBP2-24865MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-24865
Azide and BSA Free	NBP2-24865SS

compare

(select up to 3 products from this page to compare)

NB100-64852

CD1a Antibody (NA1/34-HLK) - BSA Free

(1 Review)

(9 Publications)

0.1 mg

NB100-64852

Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - mmunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Low power

Flow Cytometry: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Analysis using the Biotin conjugate of NB100-64852. Staining of MOLT 4 cells with CD1a antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-64852AF350
Alexa Fluor 405	NB100-64852AF405
Alexa Fluor 488	NB100-64852AF488
Alexa Fluor 532	NB100-64852AF532
Alexa Fluor 594	NB100-64852AF594
Alexa Fluor 647	NB100-64852AF647
Alexa Fluor 700	NB100-64852AF700
Alexa Fluor 750	NB100-64852AF750
Allophycocyanin	NB100-64852APC
Allophycocyanin/Cy7	NB100-64852APCCY7
Biotin	NB100-64852B
CoraFluor 1	NB100-64852CL1
DyLight 350	NB100-64852UV
DyLight 405	NB100-64852V
DyLight 488	NB100-64852G
DyLight 550	NB100-64852R
DyLight 594	NB100-64852DL594
DyLight 650	NB100-64852C
DyLight 680	NB100-64852FR
DyLight 755	NB100-64852IR
FITC	NB100-63529
FITC	NB100-64852F
HRP	NB100-64852H
Janelia Fluor 525	NB100-64852JF525
Janelia Fluor 549	NB100-64852JF549
Janelia Fluor 646	NB100-64852JF646
Janelia Fluor 669	NB100-64852JF669
PE	NB100-64852PE
PE/Atto594	NB100-64852PEATT594
PE/Cy5.5	NB100-64852PECY55
PE/Cy7	NB100-64852PECY7
PerCP	NB100-64852PCP
mFluor Violet 450 SE	NB100-64852MFV450
mFluor Violet 500 SE	NB100-64852MFV500
mFluor Violet 610 SE	NB100-64852MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-64852-0.025mg

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

C2CD4A Antibody - BSA Free

Neuropilin-1 Antibody [Unconjugated]

CD4 Antibody (RPA-T4) - Azide and BSA Free

TLR2 Antibody (TL2.1) - BSA Free

CD4 Antibody (GK1.5) - BSA Free

CD4 Antibody - BSA Free

MS4A4A Antibody (818112)

MS4A4A Antibody - BSA Free

CD19 Antibody (CB19) - BSA Free

MS4A3 Antibody (489433)

TLR4 Antibody (MTS510)

CD1a Antibody (NA1/34-HLK) - BSA Free