MS4A4A Antibody (818112)

HEK293 human embryonic kidney cell line co-transfected with human MS4A4A and eGFP was stained with either (A) Mouse IgG2B Isotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human MS4A4A Monoclonal Antibody (Catalog # MAB7797) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 818112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MS4A4A Antibody (818112) Summary

• Immunogen: Mouse myeloma cell line NS0 transfected with human MS4A4A
  Accession # Q96JQ5
• Specificity: Detects human MS4A4A.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MS4A4A
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MS4A4A Antibody (818112)

• 4SPAN1
• CD20 antigen-like 1
• CD20L1
• CD20L1CD20-L1
• Fc epsilon receptor beta subunit homolog
• Four-span transmembrane protein 1
• membrane-spanning 4-domains subfamily A member 4A
• membrane-spanning 4-domains, subfamily A, member 4
• membrane-spanning 4-domains, subfamily A, member 4A
• MS4A4
• MS4A4A
• MS4A4MGC22311
• MS4A7

Background

MS4A4A, also called CD20-like 1, is a 23 kDa (predicted) member of the MS4A family of four-transmembrane proteins that includes CD20 (MS4A1) and Fce RIb (MS4A2). MS4A4 cDNA is detected in hematopoietic cell lines, with highest expression in myeloid and B cell lines. Expressed sequence tags are detected in multiple organs and tissues. Human MS4A4A shares ~60% amino acid sequence identity with mouse and rat MS4A4A, if sequence gaps at the cytoplasmic N and C termini are disregarded. One potential isoform lacks aa 130-182, which includes the 3^rd transmembrane segment and the first of two extracellular regions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MS4A4A
• Uniprot:
  • Q96JQ5(Human)