Products Related to Gene-ID: 3592

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL-12 p70 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human IL-12 p70 Monoclonal Antibody (Catalog # MAB219) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12 p70 Monoclonal Antibody (Catalog # MAB219). The ND₅₀ is typically 0.3-0.9 µg/mL." class="big_lightbox"> 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12 p70 Monoclonal Antibody (Catalog # MAB219). The ND₅₀ is typically 0.3-0.9 µg/mL." class="big_thumb" />

219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA). The ND₅₀ is typically 5-50 ng/mL." class="big_lightbox"> 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA). The ND₅₀ is typically 5-50 ng/mL." alt="Recombinant Human IL-12 (219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA). The ND₅₀ is typically 5-50 ng/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm . View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm . View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

FAB3832P) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> FAB3832P) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="PBMC's + LPS (1ug/mL;24 hrs) and monensin (3 hrs) (A) vs untreated PBMC's (B) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 Monoclonal Antibody (Catalog # mab1570) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND₅₀ is typically 0.01-0.06 µg/mL." class="big_lightbox"> 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND₅₀ is typically 0.01-0.06 µg/mL." alt="Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND₅₀ is typically 0.01-0.06 µg/mL." class="big_thumb" />