IL-12 p70/IL-12A Antibody (24910) [Unconjugated]

Recombinant Human IL‑12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB219). The ND50 is typically 0.3‑0.9 µg/mL.

IL‑12 p70 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB219) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clone: 24910
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-12 p70/IL-12A Antibody (24910) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12 p70
• Specificity: Detects human IL-12 p70 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-12 p40 homodimer or rhIL-23 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12A
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.3-0.9 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 p70/IL-12A Antibody (24910) [Unconjugated]

• CLMF p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL12 p70
• IL-12 p70
• IL-12 subunit p35
• IL-12, subunit p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. Biologically active IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12 p70/IL-12A Antibody (MAB219)(18)

Publications using MAB219

• S Suliman, M Murphy, M Musvosvi, A Gela, EW Meermeier, H Geldenhuys, C Hopley, A Toefy, N Bilek, A Veldsman, WA Hanekom, JL Johnson, WH Boom, G Obermoser, H Huang, M Hatherill, DM Lewinsohn, E Nemes, TJ Scriba MR1-Independent Activation of Human Mucosal-Associated Invariant T Cells by Mycobacteria J. Immunol., 2019;0(0):. 2019 [PMID: 31611259] (Neutralization, Human)
• Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling Sci Rep, 2016;6(0):32320. 2016 [PMID: 27586092] (Flow Cytometry, Human)
• Bacillus Calmette-Gu�rin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses J Immunol, 2016;0(0):. 2016 [PMID: 27412415] (Neutralization, Human)
• SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs Nat Immunol, 2016;0(0):. 2016 [PMID: 27111145] (Neutralization, Human)
• RB Jones, S Mueller, R O'Connor, K Rimpel, DD Sloan, D Karel, HC Wong, EK Jeng, AS Thomas, JB Whitney, SY Lim, C Kovacs, E Benko, S Karandish, SH Huang, MJ Buzon, M Lichterfel, A Irrinki, JP Murry, A Tsai, H Yu, R Geleziunas, A Trocha, MA Ostrowski, Irvi A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes PLoS Pathog, 2016;12(4):e1005545. 2016 [PMID: 27082643] (Bioassay, Human)
• Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4. Nat Immunol, 2015;16(3):318-25. 2015 [PMID: 25621826] (Neutralization, Human)
• Jo J, Tan A, Ussher J, Sandalova E, Tang X, Tan-Garcia A, To N, Hong M, Chia A, Gill U, Kennedy P, Tan K, Lee K, De Libero G, Gehring A, Willberg C, Klenerman P, Bertoletti A Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver. PLoS Pathog, 2014;10(6):e1004210. 2014 [PMID: 24967632] (Neutralization, Human)
• Blom L, Poulsen L IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures. J Immunol, 2012;189(9):4331-7. 2012 [PMID: 23028054] (Neutralization, Human)
• Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property. J. Immunol., 2009;183(12):8176-85. 2009 [PMID: 20007583] (Bioassay, Human)
• Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus. J. Exp. Med., 2009;206(4):793-805. 2009 [PMID: 19289577] (Blocking, Human)
• Miller R, Sadhukhan R, Wu C Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay. J. Immunol. Methods, 2008;334(1):134-41. 2008 [PMID: 18355834] (Neutralization, Human)
• Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation. J. Clin. Invest., 2007;117(12):3868-3878. 2007 [PMID: 18060034] (Neutralization, Human)
• Mian MF, Lauzon NM, Stampfli MR, Mossman KL, Ashkar AA Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke. J. Leukoc. Biol., 2007;83(3):774-84. 2007 [PMID: 18055568] (Neutralization, Human)
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006 [PMID: 16782408] (Neutralization, Human)
• Radstake TR, Roelofs MF, Jenniskens YM, van Riel PL, Barrera P Expression of toll-like receptors 2 and 4 in rheumatoid synovial tissue and regulation by proinflammatory cytokines interleukin-12 and interleukin-18 via interferon-gamma. Arthritis Rheum., 2004;50(12):3856-65. 2004 [PMID: 15593217] (IHC-P, Human)
• Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs. Blood, 2004;104(10):3267-75. 2004 [PMID: 15242871] (ICC, Human)
• Weigt H, Muhlradt PF, Larbig M, Krug N, Braun A The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model. J. Immunol., 2004;172(10):6080-6. 2004 [PMID: 15128792] (Neutralization, Human)
• Durali D, de Goer de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment. Blood, 2003;102(12):4084-9. 2003 [PMID: 12893768] (ICC, Human)

Bioinformatics

• Gene Symbol: IL12A