Human IL-12 p70 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-12 p70 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL12A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-12 p70 DuoSet ELISA, 15 Plate

• CLMF p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL12 p70
• IL-12 p70
• IL-12 subunit p35
• IL-12, subunit p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Publications for IL-12 p70/IL-12A (DY1270)(55)

Publications using DY1270

• Nejatfard, A;Klich, JH;Eckman, N;Bailey, SJ;Gillard, K;Mejia, DR;Ou, BS;Yan, J;Hickey, JW;Appel, EA; A Transient Immunostimulatory Niche Synergizes Adoptive and Endogenous Immunity for Enhanced Tumor Control bioRxiv : the preprint server for biology 2025-08-25 [PMID: 40909524] (Human)
• Kalkusova, K;Taborska, P;Stakheev, D;Rataj, M;Smite, S;Darras, E;Albo, J;Bartunkova, J;Vannucci, L;Smrz, D; Impaired Proliferation of CD8+ T Cells Stimulated with Monocyte-Derived Dendritic Cells Previously Matured with Thapsigargin-Stimulated LAD2 Human Mast Cells Journal of immunology research 2024-07-18 [PMID: 39056014] (Human)
• Katsoulis, O;Toussaint, M;Jackson, MM;Mallia, P;Footitt, J;Mincham, KT;Meyer, GFM;Kebadze, T;Gilmour, A;Long, M;Aswani, AD;Snelgrove, RJ;Johnston, SL;Chalmers, JD;Singanayagam, A; Neutrophil extracellular traps promote immunopathogenesis of virus-induced COPD exacerbations Nature communications 2024-07-09 [PMID: 38982052] (Human)
• Twarog, M;Schustak, J;Xu, Y;Coble, M;Dolan, K;Esterberg, R;Huang, Q;Saint-Geniez, M;Bao, Y; TNF? induced by DNA-sensing in macrophage compromises retinal pigment epithelial (RPE) barrier function Scientific reports 2023-09-02 [PMID: 37660150] (Human)
• M Jovanovic, S Sekulic, M Jocic, M Jurisevic, N Gajovic, M Jovanovic, N Arsenijevi, M Jovanovic, M Mijailovic, M Milosavlje, I Jovanovic Increased Pro Th1 And Th17 Transcriptional Activity In Patients With Severe COVID-19 International Journal of Medical Sciences, 2023-02-21;20(4):530-541. 2023-02-21 [PMID: 37057213] (Human)
• D Tortolani, C Di Meo, S Standoli, F Ciaramella, S Kadhim, E Hsu, C Rapino, M Maccarrone Rare Phytocannabinoids Exert Anti-Inflammatory Effects on Human Keratinocytes via the Endocannabinoid System and MAPK Signaling Pathway International Journal of Molecular Sciences, 2023-02-01;24(3):. 2023-02-01 [PMID: 36769042] (Human)
• D Kabelitz, M Zarobkiewi, M Heib, R Serrano, M Kunz, G Chitadze, D Adam, C Peters Signal strength of STING activation determines cytokine plasticity and cell death in human monocytes Scientific Reports, 2022-10-24;12(1):17827. 2022-10-24 [PMID: 36280676] (Human)
• A Rosewell S, C Porter, G Biegert, L Jatta, M Suzuki HydrAd: A Helper-Dependent Adenovirus Targeting Multiple Immune Pathways for Cancer Immunotherapy Oncogene, 2022-06-02;14(11):. 2022-06-02 [PMID: 35681750] (Human)
• L Homann, M Rentschler, E Brenner, K Böhm, M Röcken, T Wieder IFN-gamma and TNF Induce Senescence and a Distinct Senescence-Associated Secretory Phenotype in Melanoma Cells, 2022-04-30;11(9):. 2022-04-30 [PMID: 35563820] (Human)
• A Laustsen, RM van der Sl, A Gris-Olive, SS Hernández, E Cemalovic, HQ Tang, LH Pedersen, N Uldbjerg, MR Jakobsen, RO Bak Ascorbic acid supports ex vivo generation of plasmacytoid dendritic cells from circulating hematopoietic stem cells Elife, 2021-09-02;10(0):. 2021-09-02 [PMID: 34473049] (Human)
• RP Madeira, LM Dal'Mas Ro, P de Cássia, C Mady, BM Ianni, AC Torrecilha New Biomarker in Chagas Disease: Extracellular Vesicles Isolated from Peripheral Blood in Chronic Chagas Disease Patients Modulate the Human Immune Response Journal of Immunology Research, 2021-01-11;2021(0):6650670. 2021-01-11 [PMID: 33506056] (Human)
• SK Biswas, M Mittal, E Sinha, V Singh, N Arela, B Bajaj, PK Tiwari, VM Katoch, KK Mohanty Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India Journal of Immunology Research, 2020-12-16;2020(0):1019639. 2020-12-16 [PMID: 33381602] (Human)
• M De, A Bhushan, S Chinnaswam Monocytes differentiated into macrophages and dendritic cells in the presence of human IFN-&lambda3 or IFN-&lambda4 show distinct phenotypes J Leukoc Biol, 2020-11-17;0(0):. 2020-11-17 [PMID: 33205487] (Human)
• ME Morrissey, R Byrne, C Nulty, NH McCabe, N Lynam-Lenn, CT Butler, S Kennedy, D O'Toole, J Larkin, P McCormick, B Mehigan, MC Cathcart, J Lysaght, JV Reynolds, EJ Ryan, MR Dunne, J O'Sullivan The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation BMC Cancer, 2020-06-17;20(1):566. 2020-06-17 [PMID: 32552799] (Human)
• Z Mohamed Am, MA Che Ani, SW Tan, SK Yeap, NB Alitheen, SUF Syed Najmu, J Kalyanasun, SC Chan, A Veerakumar, SL Chia, K Yusoff Evaluation of a Recombinant Newcastle Disease Virus Expressing Human IL12 against Human Breast Cancer Sci Rep, 2019-09-30;9(1):13999. 2019-09-30 [PMID: 31570732] (Chicken, Human)
• M Pascual-Ga, E Bonfill-Te, E Planas-Rig, C Rubio-Pere, R Iurlaro, A Arias, I Cuartas, A Sala-Hojma, L Escudero, F Martínez-R, I Huber-Ruan, P Nuciforo, L Pedrosa, C Marques, I Braña, E Garralda, M Vieito, M Squatrito, E Pineda, F Graus, C Espejo, J Sahuquillo, J Tabernero, J Seoane LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy Nat Commun, 2019-06-11;10(1):2416. 2019-06-11 [PMID: 31186412] (Human)
• R Gramlich, E Aliahmadi, M Peiser In Vitro Induction of T Helper 17 Cells by Synergistic Activation of Human Monocyte-Derived Langerhans Cell-Like Cells with Bacterial Agonists Int J Mol Sci, 2019-03-19;20(6):. 2019-03-19 [PMID: 30893757] (Human)
• I Ugele, ZE Cárdenas-C, K Hammon, M Wehrstein, C Bruss, K Peter, K Singer, E Gottfried, J Boesch, P Oefner, K Dettmer, K Renner, M Kreutz D-2-Hydroxyglutarate and L-2-Hydroxyglutarate Inhibit IL-12 Secretion by Human Monocyte-Derived Dendritic Cells Int J Mol Sci, 2019-02-10;20(3):. 2019-02-10 [PMID: 30744183] (Human)
• B Walch-Rück, R Ströder, L Theobald, J Pahne-Zepp, S Hegde, YJ Kim, RM Bohle, I Juhasz-Bös, EF Solomayer, S Smola Cervical cancer-instructed stromal fibroblasts enhance IL-23 expression in dendritic cells to support expansion of Th17 cells Cancer Res., 2019-01-29;0(0):. 2019-01-29 [PMID: 30696656] (Human)
• AO Idowu, S Bhattachar, S Gradus, W Oyibo, Z George, C Black, J Igietseme, AA Azenabor Plasmodium falciparum Treated with Artemisinin-based Combined Therapy Exhibits Enhanced Mutation, Heightened Cortisol and TNF-? Induction Int J Med Sci, 2018-09-07;15(13):1449-1457. 2018-09-07 [PMID: 30443164] (Human)
• A Laustsen, RO Bak, C Krapp, L Kjær, JH Egedahl, CC Petersen, S Pillai, HQ Tang, N Uldbjerg, M Porteus, NR Roan, M Nyegaard, PW Denton, MR Jakobsen Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function Nat Commun, 2018-08-30;9(1):3525. 2018-08-30 [PMID: 30166549] (Human)
• T Nakajima, H Yoshifuji, M Shimizu, K Kitagori, K Murakami, R Nakashima, Y Imura, M Tanaka, K Ohmura, F Matsuda, C Terao, T Mimori A novel susceptibility locus in the IL12B region is associated with the pathophysiology of Takayasu arteritis through IL-12p40 and IL-12p70 production Arthritis Res. Ther., 2017-09-06;19(1):197. 2017-09-06 [PMID: 28874185] (Human)
• A Mangas-Los, R García-Gar, A Urios, D Escudero-G, J Tosca, R Giner-Durá, MA Serra, C Montoliu, V Felipo Minimal hepatic encephalopathy is associated with expansion and activation of CD(4+)CD28(-), Th22 and Tfh and B lymphocytes Sci Rep, 2017-07-27;7(1):6683. 2017-07-27 [PMID: 28751644] (Human)
• Philipp Kotthoff, Annkristin Heine, Stefanie Andrea Erika Held, Peter Brossart Dexamethasone induced inhibition of Dectin-1 activation of antigen presenting cells is mediated via STAT-3 and NF-?B signaling pathways. Scientific Reports, 2017-07-03;0(0):2045-2322. 2017-07-03 [PMID: 28674449] (Human)
• YA Hayman, LR Sadofsky, JD Williamson, SP Hart, AH Morice The effects of exogenous lipid on THP-1 cells: an in vitro model of airway aspiration? ERJ Open Res, 2017-03-20;3(1):. 2017-03-20 [PMID: 28344981] (Human)
• RP Timoteo, MV da Silva, CB Miguel, DA Silva, JD Catarino, V Rodrigues, H Sales-Camp, CJ Freire Oli Th1/Th17-Related Cytokines and Chemokines and Their Implications in the Pathogenesis of Pemphigus Vulgaris Mediators Inflamm., 2017-02-22;2017(0):7151285. 2017-02-22 [PMID: 28321152] (Human)
• Acanthamoeba castellanii (Genotype T4) Stimulates the Production of Interleukin-10 as well as Pro-inflammatory Cytokines in THP-1 Cells, Human Peripheral Blood Mononuclear Cells and Human Monocyte-Derived Macrophages Infect Immun, 2016-09-19;0(0):. 2016-09-19 [PMID: 27481240] (Human)
• Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype Sci Rep, 2016-07-19;6(0):29712. 2016-07-19 [PMID: 27431276] (Human)
• Chene A, d'Almeida S, Blondy T, Tabiasco J, Deshayes S, Fonteneau J, Cellerin L, Delneste Y, Gregoire M, Blanquart C Pleural Effusions from Patients with Mesothelioma Induce Recruitment of Monocytes and Their Differentiation into M2 Macrophages. J Thorac Oncol, 2016-07-12;11(10):1765-73. 2016-07-12 [PMID: 27418105] (Human)
• Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. J Immunol, 2015-08-14;195(6):2818-28. 2015-08-14 [PMID: 26276870] (Human)
• Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production. PLoS ONE, 2015-03-20;10(3):e0120948. 2015-03-20 [PMID: 25793979] (Human)
• Jaguin M, Fardel O, Lecureur V Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2. PLoS ONE, 2015-02-24;10(2):e0116560. 2015-02-24 [PMID: 25710172] (Human)
• Wu J, Hsu H, Chiu Y, Chen H, Ni Y, Chang M The effects of cytokines on spontaneous hepatitis B surface antigen seroconversion in chronic hepatitis B virus infection. J Immunol, 2014-12-12;194(2):690-6. 2014-12-12 [PMID: 25505294] (Human)
• Tudor C, Bruns H, Daniel C, Distel L, Hartmann A, Gerbitz A, Buettner M Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma. PLoS ONE, 2014-12-03;9(12):e114345. 2014-12-03 [PMID: 25470820] (Human)
• Wang L, Deng Q, Wang J, Bai X, Xiao X, Lv H, Zhao M, Liu P Effect of CIK on multidrug-resistance reversal and increasing the sensitivity of ADR in K562/ADR cells. Oncol Lett, 2014-07-10;8(4):1778-1782. 2014-07-10 [PMID: 25202409] (Human)
• O'Toole A, Michielsen A, Nolan B, Tosetto M, Sheahan K, Mulcahy H, Winter D, Hyland J, O'Connell P, Fennelly D, O'Donoghue D, O'Sullivan J, Doherty G, Ryan E Tumour microenvironment of both early- and late-stage colorectal cancer is equally immunosuppressive. Br J Cancer, 2014-07-24;111(5):927-32. 2014-07-24 [PMID: 25058349] (Human)
• Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy. PLoS ONE, 2014-06-16;9(6):e99612. 2014-06-16 [PMID: 24932916] (Human)
• Tanaka K, Ohgo Y, Katayanagi Y, Yasui K, Hiramoto S, Ikemoto H, Nakata Y, Miyoshi N, Isemura M, Ohashi N, Imai S Anti-inflammatory effects of green soybean extract irradiated with visible light. Sci Rep, 2014-04-22;4(0):4732. 2014-04-22 [PMID: 24751752] (Human)
• Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells. Br J Haematol, 2014-01-15;165(1):134-44. 2014-01-15 [PMID: 24428589] (Human)
• Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway. PLoS ONE, 2013-12-30;8(12):e82867. 2013-12-30 [PMID: 24386121] (Human)
• Giordani L, Del Pinto T, Vincentini O, Felli C, Silano M, Viora M Two wheat decapeptides prevent gliadin-dependent maturation of human dendritic cells. Exp Cell Res, 2013-11-19;321(2):248-54. 2013-11-19 [PMID: 24262078] (Human)
• Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans. J. Bone Miner. Res., 2011-08-01;26(8):1891-903. 2011-08-01 [PMID: 21509823] (Human)
• Ochiel DO, Ghosh M, Fahey JV Human uterine epithelial cell secretions regulate dendritic cell differentiation and responses to TLR ligands. J. Leukoc. Biol., 2010-04-12;88(3):435-44. 2010-04-12 [PMID: 20385795] (Human)
• Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property. J. Immunol., 2009-12-15;183(12):8176-85. 2009-12-15 [PMID: 20007583] (Human)
• Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 2009-03-02;119(10):1424-32. 2009-03-02 [PMID: 19255340] (Human)
• Alvarado-Navarro A, Montoya-Buelna M, Munoz-Valle JF, Lopez-Roa RI, Guillen-Vargas C, Fafutis-Morris M The 3'UTR 1188 A/C polymorphism in the interleukin-12p40 gene (IL-12B) is associated with lepromatous leprosy in the west of Mexico. Immunol. Lett., 2008-04-28;118(2):148-51. 2008-04-28 [PMID: 18485489] (Human)
• Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells. FASEB J., 2008-03-25;22(8):2747-57. 2008-03-25 [PMID: 18364398] (Human)
• Matalka KZ, Ali D, Khawad AE, Qa'dan F The differential effect of Eriobotrya japonica hydrophilic leaf extract on cytokines production and modulation. Cytokine, 2007-11-26;40(3):235-40. 2007-11-26 [PMID: 18036829] (Human)
• Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J. Clin. Invest., 2007-12-01;117(12):3664-3672. 2007-12-01 [PMID: 17992260] (Human)
• Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells. Blood, 2007-09-11;110(13):4319-30. 2007-09-11 [PMID: 17848619] (Human)
• Lauzon NM, Mian F, MacKenzie R, Ashkar AA The direct effects of Toll-like receptor ligands on human NK cell cytokine production and cytotoxicity. Cell. Immunol., 2006-10-17;241(2):102-12. 2006-10-17 [PMID: 17049504] (Human)
• Macagno A, Molteni M, Rinaldi A A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression. J. Exp. Med., 2006-05-22;203(6):1481-92. 2006-05-22 [PMID: 16717116] (Human)
• Fritz JH, Girardin SE, Fitting C, Werts C, Mengin-Lecreulx D, Caroff M, Cavaillon JM, Philpott DJ, Adib-Conquy M Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists. Eur. J. Immunol., 2005-08-01;35(8):2459-70. 2005-08-01 [PMID: 16021602] (Human)
• Weigt H, Muhlradt PF, Larbig M, Krug N, Braun A The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model. J. Immunol., 2004-05-15;172(10):6080-6. 2004-05-15 [PMID: 15128792] (Human)
• Asselin-Paturel C, Brizard G, Pin JJ, Briere F, Trinchieri G Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody. J. Immunol., 2003-12-15;171(12):6466-77. 2003-12-15 [PMID: 14662846] (Mouse)

FAQs for IL-12 p70/IL-12A (DY1270). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL12A