Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96878SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1983-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59627SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF934-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-167SS | |||
| Azide and BSA Free | NBP2-80826 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB484-SP | |||
| Carrier Free | MAB484-500 | |||
| Carrier Free | FAB484V-100UG | |||
| Carrier Free | FAB484U-100UG | |||
| Carrier Free | FAB484T-100UG | |||
| Carrier Free | FAB484S-100UG | |||
| Carrier Free | FAB484R-100UG | |||
| Carrier Free | FAB484N-100UG | |||
| Carrier Free | FAB484G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF370-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-442SS | |||
| Azide and BSA Free | NBP2-80727 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC5868F | |||
| Carrier Free | IC5868P | |||
| Carrier Free | IC5868A | |||
| Carrier Free | AF5868-SP | |||
| Carrier Free | IC5868N-100UG | |||
![Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-395-img0018.jpg "Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NB100-395] - PARP activity affects 53BP1 recruitment to low-input power damage. Immunofluorescent localization of MDC1 and 53BP1 in HeLa cells irradiated with 15% (indicated by white arrowheads) and/or 30% (indicated by yellow arrowheads) at 30 min p.i. N = 10 with consistent results. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv976), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-395-img0020.jpg)
![Western Blot: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-395-img0016.jpg)
![Immunoprecipitation: MDC1 Antibody [NB100-395] - Detection of human MDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for IP at 3 ug per reaction. MDC1 was also immunoprecipitated by two other rabbit anti-MDC1 antibodies. For blotting immunoprecipitated MDC1, NB100-395 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunoprecipitation-NB100-395-img0019.jpg)
![Western Blot: MDC1 Antibody [NB100-395] - BRUCE UBC domain is required for NBS1 repair foci formation.U2OS-shBRUCE cells with stable expression of BRUCE or BRUCE mutants as indicated to the left were treated with DOX to knockdown endogenous BRUCE. After irradiation, cells were fixed & immunofluorescence stained for NBS1 (A) & quantified for cells with more than five nuclear foci (B). Bars, 10 μm. Error bars represent standard deviation from a triplicate of a representative experiment. DOX treated shBRUCE-U2OS cells were irradiated & the cell lysates subject to immunoblotting with the indicated antibodies showing the protein levels of ATM, MDC1 & NBS1 are not reduced post BRUCE knockdown (C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26683461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-395_rabbit-polyclonal-mdc1-antibody-31020241534531.jpg)
![Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody (M07), clone 5F12. Lane 1: PCBP2 transfected lysatE (38.7 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PCBP2-Antibody-5F12-Western-Blot-H00005094-M07-img0012.jpg "Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody (M07), clone 5F12. Lane 1: PCBP2 transfected lysatE (38.7 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of monoclonal antibody to PCBP2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PCBP2-Antibody-5F12-Immunocytochemistry-Immunofluorescence-H00005094-M07-img0008.jpg)
![Immunohistochemistry-Paraffin: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of monoclonal antibody to PCBP2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/PCBP2-Antibody-5F12-Immunohistochemistry-Paraffin-H00005094-M07-img0010.jpg)
![Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Western blot analysis of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi or non-transfected control. Blot probed with H00005094-M07. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/PCBP2-Antibody-5F12-Western-Blot-H00005094-M07-img0005.jpg)
![Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of PCBP2 expression in K-562 (Cat # L009V1).](http://images.novusbio.com/fullsize/PCBP2-Antibody-5F12-Western-Blot-H00005094-M07-img0011.jpg)
![ELISA: PCBP2 Antibody (5F12) [H00005094-M07] - Detection limit for recombinant GST tagged PCBP2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PCBP2-Antibody-5F12-ELISA-H00005094-M07-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DNA/RNA Damage Antibody (15A3) [NB110-96878] - visualized on a retinal injury](http://images.novusbio.com/fullsize/DNA-RNA-Damage-Antibody-15A3-Immunocytochemistry-Immunofluorescence-NB110-96878-img0004.jpg "Immunocytochemistry/Immunofluorescence: DNA/RNA Damage Antibody (15A3) [NB110-96878] - visualized on a retinal injury")
![Immunohistochemistry: DNA/RNA Damage Antibody (15A3) [NB110-96878] - Immunohistochemistry analysis using Mouse Anti-DNA/RNA DamageMonoclonal Antibody, Clone 15A3 (NB110-96878). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-DNA/RNA DamageMonoclonal Antibody (NB110-96878) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial.](http://images.novusbio.com/fullsize/DNA-RNA-Damage-Antibody-15A3-Immunohistochemistry-NB110-96878-img0013.jpg)
![DNA/RNA Damage Antibody (15A3) [NB110-96878] - Immunohistochemistry analysis using Mouse Anti-DNA/RNA DamageMonoclonal Antibody, Clone 15A3 (NB110-96878). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-DNA/RNA DamageMonoclonal Antibody (NB110-96878) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/DNA-RNA-Damage-Antibody-15A3-NB110-96878-img0014.jpg)
![Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Western Blot analysis of Rat brain membrane lysate showing detection of CaM Kinase II alpha protein using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:1000.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Western-Blot-NB100-1983-img0015.jpg "Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Western Blot analysis of Rat brain membrane lysate showing detection of CaM Kinase II alpha protein using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunocytochemistry-Immunofluorescence-NB100-1983-img0018.jpg)
![Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunohistochemistry analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunohistochemistry-NB100-1983-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunocytochemistry-Immunofluorescence-NB100-1983-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunocytochemistry-Immunofluorescence-NB100-1983-img0014.jpg)
![Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Immunohistochemistry analysis using Mouse Anti-CaM Kinase II alpha Monoclonal Antibody, Clone 6G9 (NB100-1983). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaM Kinase II alpha Monoclonal Antibody (NB100-1983) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-6G9-Immunohistochemistry-NB100-1983-img0016.jpg)
![Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)](http://images.novusbio.com/fullsize/LANA2-Antibody-CM-A807-Western-Blot-NB200-167-img0003.jpg "Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)")
![Immunohistochemistry-Paraffin: LANA2 Antibody (CM-A807) [NB200-167] - vIRF3 expression in KS tissues. We obtained KS tissue microarrays from AIDS Cancer Specimen Resource and performed immunohistochemistry, visualized by Aperio F.L. digital pathological scanning. A biopsy sample of KS TMA was stained with anti-LANA. Representative image shows the LANA staining of skin KS lesion. Image collected and cropped by CiteAb from the following publication (//mbio.asm.org/lookup/doi/10.1128/mBio.02217-17), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LANA2-Antibody-CM-A807-Immunohistochemistry-Paraffin-NB200-167-img0004.jpg)
![Immunohistochemistry: LANA2 Antibody (CM-A807) - BSA Free [NB200-167] - vIRF3 expression in KS tissues. We obtained KS tissue microarrays from AIDS Cancer Specimen Resource & performed immunohistochemistry, visualized by Aperio F.L. digital pathological scanning. A biopsy sample of KS TMA was stained with anti-LANA & anti-vIRF3 (CM-A807). Representative images show the LANA staining of skin KS lesion & the vIRF3 staining of skin, tonsil, & mouth KS lesions & positive staining corresponding to either LANA or vIRF3 in total KS biopsy specimens embedded in TMA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29339432), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-167_mouse-monoclonal-lana2-antibody-cm-a807-310202415371954.jpg)
![Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Chicken erythrocytes (1% v/v), isolated by density gradient centrifugation, were transiently transfected with a Flag-tagged chicken ART4 containing plasmid or the empty plasmid (pSecTag B), and incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4) or an anti-Flag antibody and the respective isotype control stained with the 1G4 antibody. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ethenoadenosine-Antibody-1G4-Flow-Cytometry-NB600-442-img0002.jpg "Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Chicken erythrocytes (1% v/v), isolated by density gradient centrifugation, were transiently transfected with a Flag-tagged chicken ART4 containing plasmid or the empty plasmid (pSecTag B), and incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4) or an anti-Flag antibody and the respective isotype control stained with the 1G4 antibody. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license.")
![Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Detection of ADP-ribosylated proteins by flow cytometry. C-33A cells (1 x 10^7/mL), transiently transfected with a Flag-tagged chicken ART4 containing plasmid, were incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4). Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ethenoadenosine-Antibody-1G4-Flow-Cytometry-NB600-442-img0001.jpg)
![Flow Cytometry: Ethenoadenosine Antibody (1G4) [NB600-442] - Etheno-ADP-ribosylation of cell-surface proteins on C3H-10T1/2 cells. C3H-10T 1/2 cells at different stages of differentiation (progenitors, MyoD-myoblasts, myotubes) were incubated for 30 minutes in the absence (dashed lines) or presence (solid lines) of 100 uM etheno-NAD. Cells were then washed, incubated with the primary monoclonal antibody 1G4, stained with a FITC-conjugated goat-anti-mouse secondary antibody and subjected to FACS analysis. Data show one representative experiments out of three. Image collected and cropped by CiteAb from the following publication (//bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-91), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ethenoadenosine-Antibody-1G4-Flow-Cytometry-NB600-442-img0003.jpg)
![Western Blot: BID Antibody [NB100-56106] - Analysis of Bid in normal mouse tissues using this antibody. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kDa Bid and the ~15 kDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).](http://images.novusbio.com/fullsize/BID-Antibody-Western-Blot-NB100-56106-img0008.jpg "Western Blot: BID Antibody [NB100-56106] - Analysis of Bid in normal mouse tissues using this antibody. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kDa Bid and the ~15 kDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).")
![Immunohistochemistry-Paraffin: BID Antibody [NB100-56106] - Two cores from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray demonstrate the variable expression of Bid protein. The sections were stained with Bid antibody at 1:2000 with hematoxylin-eosin counterstain. Section A shows very weak staining while section B stains much more strongly.](http://images.novusbio.com/fullsize/BID-Antibody-Immunohistochemistry-Paraffin-NB100-56106-img0009.jpg)
![Western Blot: BID Antibody [NB100-56106] - Analysis of BID in NRK whole cell lysate using anti-BID antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/BID-Antibody-Western-Blot-NB100-56106-img0007.jpg)
![Immunohistochemistry-Paraffin: BID Antibody [NB100-56106] - Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using this antibody at 1:2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.](http://images.novusbio.com/fullsize/BID-Antibody-Immunohistochemistry-Paraffin-NB100-56106-img0010.jpg)
