CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: Flow
• Clone: 145-2C11
• Clonality: Monoclonal
• Host: Hamster
• Conjugate: Alexa Fluor 488
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 488] Summary

• Immunogen: H-2K^b-specific mouse cytotoxic T-lymphocyte
• Specificity: Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.¹ Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.^3,4
• Isotype: IgG
• Clonality: Monoclonal
• Host: Hamster
• Gene: Cd247

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 epsilon Antibody (145-2C11) [Alexa Fluor® 488]

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.⁴ The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.¹ 145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.^{6, 7}

1. Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374.
2. Portoles, P. et al. (1989) J. Immunol. 142:4169.
3. Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703.
4. Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583.
5. Small, M. et al. (1994) J. Immunol. Meth. 167:103.
6. Alegre, M.L. et al. (1991) J. Immunol. 146:1184.
7. Hendrickson, M. et al. (1995) Transplantation 60:828.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Cd247