Western Blot: 2A Peptide Antibody (3H4) [NBP2-59627] - Total protein from SpCas9-T2A peptide transfected HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein G purified
Alternate Names for 2A Peptide Antibody (3H4)
2A cleavage peptide
2A Peptide Antibody
2A peptide tag
antibody for 2A-containing recombinant proteins
FMDV 2A-peptide antibody
P2A tagged proteins
peptide 2A sequences
self-cleaving 2A peptide
T2A tagged proteins
Thosea asigna virus 2A antibody
Thosea asigna virus 2A
Initially discovered from the foot-and-mouth disease virus (FMDV, a picornavirus), 2A peptide is a self-cleaving peptide 18-22 amino acids long with a conserved C-terminal motif Asp-Val/Ile-Glu-X-Asn-Pro-Gly-Pro. All picornaviruses have 2A peptides but they differ in size and function. The FMDV genome is a single open reading frame encoding a polyprotein and 2A denotes a specific cleavage region within the polyprotein. Co-translational cleavage of the FMDV polyprotein is not mediated by a proteolytic mechanism but rather by "ribosomal skipping" or "StopGO" at the 2A/2B junction. When synthesis terminates at a sense codon, the polypeptide is released before resuming translation at the next codon (1, 2).
Developed for genetic engineering, 2A cleavage has been shown to function in a wide range of eukaryotic cells, and in vivo (3). The 2A peptide is inserted between the coding sequences of at least two genes, enabling the simultaneous expression of multiple proteins from a single plasmid construct. Four variants of 2A peptides have been used in gene expression systems including FMDV 2A (F2A), equine rhinitis A virus 2A (E2A), porcine teschovirus-1 2A (P2A) or Thoseaasigna virus 2A (T2A). Compared to other multi-gene co-expression systems such as IRES, 2A peptides results in stoichiometric expression of different proteins. However, cleaving efficiency and protein expression using the different 2A peptides varies, requiring optimization of polycistronic constructs for experiments.
1. Liu Z, Chen O, Wall J, Zheng M, Zhou Y, Wang L, Vaseghi H, Qian L, Liu J. (2017). Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Scientific Reports. 7. 10.1038/s41598-017-02460-2.
2. Luke GA, Escuin H, Felipe PD, Ryan MD. (2009) 2A to the Fore - Research, Technology and Applications, Biotechnology and Genetic Engineering Reviews. 26(1): 223-260.
3. Markus BM, Bell GW, Lorenzi HA, Lourido S. (2019) Optimizing Systems for Cas9 Expression in Toxoplasma gondii. mSphere. 4(3): e00386-19.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for 2A Peptide Antibody (NBP2-59627)(6)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 3 applications: ICC/IF, IHC, WB.
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Showing Publications 1 - 6 of 6.
Publications using NBP2-59627
Krabbe S, Paradiso E, d'Aquin S et al. Adaptive disinhibitory gating by VIP interneurons permits associative learning bioRxiv Oct 15 2018 (IHC, Mouse)
Bonny A Investigating mechanisms of endogenous, systems-level coordination in stress adaptation and homeostasis Thesis Jan 1 2020 (WB, Human)
FAQs for 2A Peptide Antibody (NBP2-59627). (Showing 1 - 2 of 2 FAQs).
Are there any cleavage sites on 2A peptide, Clone 3H4?
Scientific articles have referenced the recognition of 2A sequence derived from foot and mouth piconavirus (VKQTLNFDLLKLAGDVESNPG*P) with cleavage site between G and P.
How long are the 2A sequences and does it encode more than one protein?
The sequence is approximately 20 amino acids long based on the source of virus. 2A peptides allow several proteins to be encoded as polyproteins, which then separate into component proteins upon translation.
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