2A Peptide Antibody (3H4)

Images

 
Western Blot: 2A Peptide Antibody (3H4) [NBP2-59627] - Total protein from SpCas9-T2A peptide transfected HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ICC/IF, IP
Clone
3H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

2A Peptide Antibody (3H4) Summary

Immunogen
This 2A Peptide Antibody (3H4) was developed against KLH-coupled synthetic peptide corresponding to 2A peptide of Thosea asigna virus (CGDVEENPG)
Specificity
This 2A Peptide Antibody (3H4) peptide antibody is specific for T2A and P2A tagged proteins. Specificity to F2A and E2A proteins has not been tested.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Reviewed Applications
Read 1 Review rated 4
using
NBP2-59627 in the following applications:

Publications
Read Publications using
NBP2-59627 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for 2A Peptide Antibody (3H4)

  • 2A cleavage peptide
  • 2A Peptide Antibody
  • 2A peptide tag
  • 2A peptide
  • 2A sequence
  • 2A-like peptide
  • antibody for 2A-containing recombinant proteins
  • FMDV 2A-peptide antibody
  • FMDV 2A-peptide
  • MDV 2A-peptide
  • P2A peptide
  • P2A tagged proteins
  • peptide 2A sequences
  • self-cleaving 2A peptide
  • T2A peptide
  • T2A tagged proteins
  • Thosea asigna virus 2A antibody
  • Thosea asigna virus 2A

Background

Initially discovered from the foot-and-mouth disease virus (FMDV, a picornavirus), 2A peptide is a self-cleaving peptide 18-22 amino acids long with a conserved C-terminal motif Asp-Val/Ile-Glu-X-Asn-Pro-Gly-Pro. All picornaviruses have 2A peptides but they differ in size and function. The FMDV genome is a single open reading frame encoding a polyprotein and 2A denotes a specific cleavage region within the polyprotein. Co-translational cleavage of the FMDV polyprotein is not mediated by a proteolytic mechanism but rather by "ribosomal skipping" or "StopGO" at the 2A/2B junction. When synthesis terminates at a sense codon, the polypeptide is released before resuming translation at the next codon (1, 2).

Developed for genetic engineering, 2A cleavage has been shown to function in a wide range of eukaryotic cells, and in vivo (3). The 2A peptide is inserted between the coding sequences of at least two genes, enabling the simultaneous expression of multiple proteins from a single plasmid construct. Four variants of 2A peptides have been used in gene expression systems including FMDV 2A (F2A), equine rhinitis A virus 2A (E2A), porcine teschovirus-1 2A (P2A) or Thoseaasigna virus 2A (T2A). Compared to other multi-gene co-expression systems such as IRES, 2A peptides results in stoichiometric expression of different proteins. However, cleaving efficiency and protein expression using the different 2A peptides varies, requiring optimization of polycistronic constructs for experiments.

References

1. Liu Z, Chen O, Wall J, Zheng M, Zhou Y, Wang L, Vaseghi H, Qian L, Liu J. (2017). Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Scientific Reports. 7. 10.1038/s41598-017-02460-2.

2. Luke GA, Escuin H, Felipe PD, Ryan MD. (2009) 2A to the Fore - Research, Technology and Applications, Biotechnology and Genetic Engineering Reviews. 26(1): 223-260.

3. Markus BM, Bell GW, Lorenzi HA, Lourido S. (2019) Optimizing Systems for Cas9 Expression in Toxoplasma gondii. mSphere. 4(3): e00386-19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 2A Peptide Antibody (NBP2-59627)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


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ICC/IF
(1)
IHC
(2)
WB
(3)
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(2)
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(3)
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Showing Publications 1 - 6 of 6.
Publications using NBP2-59627 Applications Species
Krabbe S, Paradiso E, d'Aquin S et al. Adaptive disinhibitory gating by VIP interneurons permits associative learning bioRxiv Oct 15 2018 (IHC, Mouse) IHC Mouse
Bonny A Investigating mechanisms of endogenous, systems-level coordination in stress adaptation and homeostasis Thesis Jan 1 2020 (WB, Human) WB Human
Song D, Wang D, Yang Q et al. The lateralization of left hippocampal CA3 during the retrieval of spatial working memory Nat Commun Jun 9 2020 [PMID: 32518226] (ICC/IF, Mouse) ICC/IF Mouse
Yu FH, Huang KJ, Wang CT HIV-1 Mutant Assembly, Processing and Infectivity Expresses Pol Independent of Gag Viruses Jan 2 2020 [PMID: 31906562] (WB, Human) WB Human
Markus BM, Bell GW, Lorenzi HA, Lourido S, Optimizing Systems for Cas9 Expression in Toxoplasma gondii mSphere Jun 26 2019 [PMID: 31243081] (WB) WB
Krabbe S, Paradiso E, d'Aquin S et al. Adaptive disinhibitory gating by VIP interneurons permits associative learning Cellular and Molecular Gastroenterology and Hepatology Sep 1 2018 (IHC, Mouse) IHC Mouse

Review for 2A Peptide Antibody (NBP2-59627) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-59627:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot 2A Peptide NBP2-59627
Enlarge
4
reviewed by:
Min-Joon Han
WB Human 08/14/2018
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Summary

ApplicationWestern Blot
Sample TestedHuman fibroblast
SpeciesHuman
LotA-2

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 2A Peptide Antibody (NBP2-59627). (Showing 1 - 2 of 2 FAQs).

  1. Are there any cleavage sites on 2A peptide, Clone 3H4?
    • Scientific articles have referenced the recognition of 2A sequence derived from foot and mouth piconavirus (VKQTLNFDLLKLAGDVESNPG*P) with cleavage site between G and P.
  2. How long are the 2A sequences and does it encode more than one protein?
    • The sequence is approximately 20 amino acids long based on the source of virus. 2A peptides allow several proteins to be encoded as polyproteins, which then separate into component proteins upon translation.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-59627AF350
Alexa Fluor 405 NBP2-59627AF405
Alexa Fluor 488 NBP2-59627AF488
Alexa Fluor 532 NBP2-59627AF532
Alexa Fluor 594 NBP2-59627AF594
Alexa Fluor 647 NBP2-59627AF647
Alexa Fluor 700 NBP2-59627AF700
Alexa Fluor 750 NBP2-59627AF750
Biotin NBP2-59627B
DyLight 350 NBP2-59627UV
DyLight 405 NBP2-59627V
DyLight 488 NBP2-59627G
DyLight 550 NBP2-59627R
DyLight 594 NBP2-59627DL594
DyLight 650 NBP2-59627C
DyLight 680 NBP2-59627FR
DyLight 755 NBP2-59627IR
FITC NBP2-59627F
HRP NBP2-59627H
Janelia Fluor 549 NBP2-59627JF549
Janelia Fluor 646 NBP2-59627JF646

Additional 2A Peptide Products

Array NBP2-59627

Research Areas for 2A Peptide Antibody (NBP2-59627)

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Recent Reviews

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Min-Joon Han
08/14/2018
Application: WB
Species: Human