Home » Cytokeratin 18 » Cytokeratin 18 Antibodies » Cytokeratin 18 Antibody (E431-1)

Cytokeratin 18 Antibody (E431-1)

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Summary
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    • Catalog Number
      NB110-56910
    • Availability
      Product Discontinued

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Cytokeratin 18 Antibody (E431-1) Summary

Immunogen
A synthetic peptide corresponding to residues of human Cytokeratin 18 was used as immunogen. Human Cytokeratin 18 (K18) was used as immunogen after isolation from cells pretreated with okadaic acid or pervanadate to promote Tyr hyperphosphorylation.
Marker
Epithelial Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
KRT18
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Western Blot 1:1000-10000
Application Notes
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 45kDa. Use in Rat reported in customer review.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cytokeratin 18 Overexpression Lysate
Publications
Read Publications using
NB110-56910 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
49% PBS, 0.05% BSA and 50% Glycerol
Preservative
0.01% Sodium Azide
Purity
Protein A or G purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Cytokeratin 18 Antibody (E431-1)

  • Cell proliferation-inducing gene 46 protein
  • cell proliferation-inducing protein 46
  • CK-18
  • CYK18
  • Cytokeratin 18
  • cytokeratin-18
  • K18
  • Keratin 18
  • keratin, type I cytoskeletal 18
  • keratin-18
  • KRT18

Background

Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 18 Antibody (NB110-56910)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF/IHC.


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Product General Protocols

View specific protocols for Cytokeratin 18 Antibody (NB110-56910): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 18 Antibody (NB110-56910) (0)

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Control Lysate(s)

Cytokeratin 18 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

Additional Cytokeratin 18 Products

Research Areas for Cytokeratin 18 Antibody (NB110-56910)

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Blogs on Cytokeratin 18.

Check out the latest blog posts on Cytokeratin 18.
Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT18
Entrez
Uniprot