| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22122SS | |||
| Azide and BSA Free | NBP2-80719 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33074 | |||
| IHC-Prediluted | NBP2-44976 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18961SS | |||
| Azide and BSA Free | NBP2-80896 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49532SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51843SS | |||
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NBP1-32458] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Albumin protein stained by Albumin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32458-img0021.jpg "Immunocytochemistry/Immunofluorescence: Albumin Antibody [NBP1-32458] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Albumin protein stained by Albumin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Mouse liver. Albumin stained by Albumin antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Albumin-Antibody-Immunohistochemistry-Paraffin-NBP1-32458-img0025.jpg)
![Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Human hepatocellular carcinoma. Albumin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Albumin-Antibody-Immunohistochemistry-Paraffin-NBP1-32458-img0016.jpg)
![Immunohistochemistry-Frozen: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Frozen section of rat liver. Albumin antibody diluted at 1:200.](http://images.novusbio.com/fullsize/Albumin-Antibody-Immunohistochemistry-Frozen-NBP1-32458-img0017.jpg)
![Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Rat liver. Albumin stained by Albumin antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Albumin-Antibody-Immunohistochemistry-Paraffin-NBP1-32458-img0024.jpg)
![Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Dog liver.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp1-32458_rabbit-polyclonal-albumin-antibody-immunohistochemistry-paraffin-1482023124527..jpg)
![Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Dog kidney.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp1-32458_rabbit-polyclonal-albumin-antibody-immunohistochemistry-paraffin-1482023124716..jpg)
![Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Cat liver.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp1-32458_rabbit-polyclonal-albumin-antibody-immunohistochemistry-paraffin-1482023124837..jpg)
![Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Immunofluorescence localization of TNF-alpha , IL-1 beta & IL-6 in the liver tissue of animals treated with ConA or ConA + hVNS. a–c Representative immunofluorescence images of TNF-alpha , IL-1 beta , & IL-6 that were merged with CD11b. Signal intensities of TNF-alpha , IL-1 beta , & IL-6 from 4 to 6 images were averaged per each animal & compared among experimental groups (n = 3 independent experiments). One-way ANOVA with Tukey post hoc is shown. *p Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Albumin stained by Albumin antibody (NBP1-32458) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32458_rabbit-polyclonal-albumin-antibody-91020241957221.jpg)
![Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Albumin stained by Albumin antibody (NBP1-32458) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32458_rabbit-polyclonal-albumin-antibody-91020242063392.jpg)
![Western Blot: Albumin Antibody [NBP1-32458] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32458_rabbit-polyclonal-albumin-antibody-91020242041286.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Albumin Antibody [NBP1-32458] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32458_rabbit-polyclonal-albumin-antibody-1610202419405735.jpg)
![Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0006.jpg "Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Human endometrial stromal cells stained with Endoglin/CD105 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0014.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Immunohistochemical staining of endoglin (CD105) in B16F10 murine melanoma tumor model. IHC-P image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0013.jpg)
![Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates.](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red).](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - HMSC cells stained with anti-CD105 antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-NBP2-22122-img0007.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0005.jpg)
![ELISA: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng).](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-ELISA-NBP2-22122-img0001.jpg)
![Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/USP22-Antibody-Western-Blot-NBP1-49644-img0001.jpg "Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.")
![Western Blot: USP22 Antibody [NBP1-49644] - analysis of USP22 in HeLa whole cell lysate using anti-USP22 antibody. The primary antibody was used at a dilution of 1:500 and incubated overnight at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/USP22-Antibody-Western-Blot-NBP1-49644-img0002.jpg)
![Simple Western: USP22 Antibody [NBP1-49644] - Simple Western lane view shows a specific band for USP22 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/USP22-Antibody-Simple-Western-NBP1-49644-img0003.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0010.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0008.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0001.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0003.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0004.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0005.jpg)
![Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3seconds.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Western-Blot-NB100-652-img0011.jpg "Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3seconds.")
![Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiLasu1/Ureb1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Immunohistochemistry-Paraffin-NB100-652-img0012.jpg)
![Immunoprecipitation: HUWE1 Antibody [NB100-652] - Detection of human Lasu1/Ureb1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Lasu1/Ureb1 antibody NB100-652 (lot NB100-652-9) used for IP at 6 ug per reaction. Lasu1/Ureb1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-652-8). For blotting immunoprecipitated Lasu1/Ureb1, NB100-652 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/HUWE1-Antibody-Immunoprecipitation-NB100-652-img0013.jpg)
![Western Blot: GFAP Antibody [NB100-53809] - (0.005ug/ml) staining of Human Cerebellum (A) and (0.01ug/ml) Cerebral Cortex (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB100-53809-img0002.jpg "Western Blot: GFAP Antibody [NB100-53809] - (0.005ug/ml) staining of Human Cerebellum (A) and (0.01ug/ml) Cerebral Cortex (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: GFAP Antibody [NB100-53809] - (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB100-53809-img0001.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Differential effects of the 20(R/S)-Rg3 stereoisomers on proliferation of VSMCs within plaques. A, Co-immunofluorescence staining of the aortic root for VSMCs (anti-a-SMA antibody, red) and proliferation marker PCNA (green) and DAPI (blue) Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/10.1111/jcmm.13601) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-NBP2-22120-img0016.jpg "Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Differential effects of the 20(R/S)-Rg3 stereoisomers on proliferation of VSMCs within plaques. A, Co-immunofluorescence staining of the aortic root for VSMCs (anti-a-SMA antibody, red) and proliferation marker PCNA (green) and DAPI (blue) Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/10.1111/jcmm.13601) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of HepG2 cells using (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunocytochemistry-Immunofluorescence-NBP2-22120-img0008.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis using alpha-Smooth Muscle Actin Antibody (4A4) against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate. The theoretical molecular weight of the antibody is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Western-Blot-NBP2-22120-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) using alpha-Smooth Muscle Actin Antibody (4A4) with DAB staining.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Paraffin-NBP2-22120-img0009.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Representative immunohistochemical alpha-Smooth Muscle Actin staining of mouse aortic sinus. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Frozen-NBP2-22120-img0013.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Effects of the 20(R/S)-Rg3 stereoisomers on early atherogenesis in diabetic mice. D, F, Representative immunohistochemical a-SMA staining and quantification of the plaque smooth muscle cell content (n = 3). Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/10.1111/jcmm.13601) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-NBP2-22120-img0017.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of Hela cells using alpha-Smooth Muscle Actin Antibody (4A4) (green) and negative control (purple).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Flow-Cytometry-NBP2-22120-img0007.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Representative immunohistochemical alpha-Smooth Muscle Actin staining of mouse aortic sinus. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Frozen-NBP2-22120-img0015.jpg)
![ELISA: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-ELISA-NBP2-22120-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A4) - BSA Free [NBP2-22120] - Differential effects of the 20(R/S)‐Rg3 stereoisomers on proliferation of VSMCs within plaques. A, Co‐immunofluorescence staining of the aortic root for VSMCs (anti‐ alpha ‐SMA antibody, red) & proliferation marker PCNA (green) & a bar graph summarizing the results (n = 3). Scale bar: 20 μm. B, Western blot analysis of cyclin D1, cyclin E & PCNA protein expression within plaques. The bands are quantified by densitometric analysis. Protein expression of cyclin D1 & PCNA was normalized to beta ‐actin, & expression of cyclin E was normalized to GADPH (n = 3, 4, & 4 for cyclin D1, cyclin E & PCNA, respectively). The results are expressed as the mean values ± SEM. *P < .05, **P < .01, ***P < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29566305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22120_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-4a4-310202415395945.jpg)
![Immunohistochemistry-Frozen: Oval Cell Marker Antibody (MIC1-1C3) [NBP1-18961] - Ductular reaction in injured mouse liver. MIC1-1C3 (red) and DAPI (blue). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Oval-Cell-Marker-Antibody-MIC1-1C3-Immunohistochemistry-Frozen-NBP1-18961-img0004.jpg "Immunohistochemistry-Frozen: Oval Cell Marker Antibody (MIC1-1C3) [NBP1-18961] - Ductular reaction in injured mouse liver. MIC1-1C3 (red) and DAPI (blue). IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry: Oval Cell Marker Antibody (MIC1-1C3) [NBP1-18961] - Analysis of DDC treated mouse liver.](http://images.novusbio.com/fullsize/Oval-Cell-Marker-Antibody-MIC1-1C3-Immunohistochemistry-NBP1-18961-img0003.jpg)
![Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP1-31488-img0031.jpg "<b>Biological Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.")
![<b>Biological Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP1-31488-img0033.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP1-31488] - Analysis of methanol-fixed HeLa, using CD44 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31488-img0016.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human cervical carcinoma. CD44 stained by CD44 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-31488-img0036.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human endometrial carcinoma. CD44 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-31488-img0026.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human pancreatic tumor, using CD44 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-31488-img0035.jpg)
![Immunocytochemistry/ Immunofluorescence: CD44 Antibody [NBP1-31488] - CD44 antibody detects CD44 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: CD44 stained by CD44 antibody (NBP1-31488) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-91020242091445.jpg)
![Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (NBP1-31488) diluted at 1:45000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-910202420104431.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Non-transfected (–) and transfected (+) Wild-type (WT) and G CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-1610202419405756.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-1610202419414564.jpg)
![Western Blot: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in Jurkat whole cell extracts](http://images.novusbio.com/fullsize/PGAM1-Antibody-Western-Blot-NBP1-49532-img0008.jpg "Western Blot: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in Jurkat whole cell extracts")
![Immunocytochemistry/Immunofluorescence: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in HeLa cells using NBP1-49532. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/PGAM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49532-img0005.jpg)
![Immunohistochemistry: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in mouse tongue.](http://images.novusbio.com/fullsize/PGAM1-Antibody-Immunohistochemistry-NBP1-49532-img0006.jpg)
![Simple Western: PGAM1 Antibody [NBP1-49532] - Simple Western lane view shows a specific band for PGAM1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGAM1-Antibody-Simple-Western-NBP1-49532-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution overnight at 4C and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51843-img0012.jpg "Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution overnight at 4C and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Simple Western: Aurora A Antibody [NBP1-51843] - Simple Western lane view shows a specific band for Aurora A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Simple-Western-NBP1-51843-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51843-img0008.jpg)
![Western Blot: Aurora A Antibody [NBP1-51843] - WB detection of Aurora A in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP1-51843-img0011.jpg)
![Immunohistochemistry: Aurora A Antibody [NBP1-51843] - IHC staining of Aurora A in mouse brain.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-NBP1-51843-img0003.jpg)
![Simple Western: Aurora A Antibody - BSA Free [NBP1-51843] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51843_rabbit-polyclonal-aurora-a-antibody-308202410445515.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary antibody was used at a dilution of 1:1000 in 2% goat serum in PBS and incubated for 2 hours at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Immunocytochemistry-Immunofluorescence-NB500-481-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary antibody was used at a dilution of 1:1000 in 2% goat serum in PBS and incubated for 2 hours at room temperature. Image from verified customer review.")
![CD44 Antibody (MEM-263) [NB500-481] - Analysis of CD44 in HeLa cells using anti-CD44 (MEM-263) purified; non-reducing conditions.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-NB500-481-img0009.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0003.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) APC conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0007.jpg)
![Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Expression of CD44 and CK18 in multiple clones of ovarian cancer cells. CD44+ cells also express CK18 as determined by western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jo/2011/620523/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0008.jpg)
![Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Western-Blot-NB500-481-img0002.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0004.jpg)
![Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - Staining of human peripheral blood using anti-CD44 (MEM-263) FITC conjugate.](http://images.novusbio.com/fullsize/CD44-Antibody-MEM-263-Flow-Cytometry-NB500-481-img0005.jpg)
