Oval Cell Marker Antibody (MIC1-1C3) - BSA Free

Images

 
Immunohistochemistry-Frozen: Oval Cell Marker Antibody (MIC1-1C3) [NBP1-18961] - Ductular reaction in injured mouse liver. MIC1-1C3 (red) and DAPI (blue). IHC-Fr image submitted by a verified customer review.
Immunohistochemistry: Oval Cell Marker Antibody (MIC1-1C3) [NBP1-18961] - Analysis of DDC treated mouse liver.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
MIC1-1C3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Oval Cell Marker Antibody (MIC1-1C3) - BSA Free Summary

Immunogen
Nonparenchymal cells from DDC-treated mice
Localization
Cell surface
Marker
Hepatic periductal cell Marker
Specificity
Specific for hepatic proliferating duct cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:50 - 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin
Application Notes
This Oval Cell Marker (MIC1-1C3) antibody is useful for Immunohistochemistry on acetone fixed frozen sections and Flow cytometry. Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin were reported in scientific literature. This antibody is CyTOF ready.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-18961 in the following applications:

Publications
Read Publications using
NBP1-18961 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30236451).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Background

Liver is well known to possess facultative stem cells which can be activated in response to injury and hepatic stem cell activation was first described in rat model, wherein, chemical damage in combination with partial hepatectomy resulted in the emergence of a new cell type which was described according to its nuclear morphology as "oval cells". The word 'oval cells' is now employed to define population of small proliferating cells with oval-shaped nuclei which arise within liver tissue following certain types of hepatic injury. Oval cell proliferation is activated when the proliferative capacity of hepatocytes is impaired, as well as in certain models of carcinogenesis. Histologically, hepatic tissue is highly complex and contains many different cell types including various kinds of hepatocytes, ducts, hematopoietic cells, endothelial cells, macrophages, stellate cells etc., and the oval cells have been proposed to be bipotential progenitor cells capable of generating both hepatocytes and bile ducts. Due to their bipotency and high clonogenicity, oval cells have been considered putative liver stem cells and research is in progress for elucidation of signaling molecules involved in controlling the activation, proliferation and differentiation of this unique cell population. Our oval cell antibodies have been developed against hepatic nonparenchymal cells (NPCs) from mice undergoing oval cell activation and with these antibodies, it is now possible to perform microarray analysis of distinct liver cell populations during induced oval cell response or certain pathological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oval Cell Marker Antibody (NBP1-18961)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: FLOW, Flow, ICC/IF, IHC-Fr, IHC-P.


Filter By Application
FLOW
(3)
Flow
(2)
ICC/IF
(1)
IHC-Fr
(2)
IHC-P
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(10)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-18961 Applications Species
Beel S, Kolloch L, Apken LH et al. kappa B-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis Nat Commun Jul 8 2020 [PMID: 32641778] (Flow, Mouse) Flow Mouse
Krishnan A, Katsumi T, Guicciardi ME et al. Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor Deficiency Promotes the Ductular Reaction, Macrophage Accumulation, and Hepatic Fibrosis in the Mdr2-/- Mouse Am. J. Pathol. Mar 30 2020 [PMID: 32240619] (Mouse) Mouse
Tam PKH, Yiu RS, Lendahl U, Andersson ER. Cholangiopathies - Towards a molecular understanding. EBioMedicine Sep 1 2018 [PMID: 30236451] (Human, Mouse) Human, Mouse
Lu XF, Zhou YJ, Zhang L et al. Loss of Dicer1 impairs hepatocyte survival and leads to chronic inflammation and progenitor cell activation World J. Gastroenterol. 2015 Jun 07 [PMID: 26074697] (IHC-P, Mouse) IHC-P Mouse
Dorrell C, Tarlow B et al. The organoid-initiating cells in mouse pancreas and liver are phenotypically and functionally similar. Stem Cell Res Jan 9 2014 [PMID: 25151611] (Flow, Mouse) Flow Mouse
Bird TG, Lu WY, Boulter L et al. Bone marrow injection stimulates hepatic ductular reactions in the absence of injury via macrophage-mediated TWEAK signaling Proc Natl Acad Sci U S A 2013 Apr 1 [PMID: 23576749] (IHC-P, ICC/IF, Mouse) IHC-P, ICC/IF Mouse
Dorrell C, Erker L, Schug J, Kopp JL, Canaday PS, Fox AJ, Smirnova O, Duncan AW, Finegold MJ, Sander M, Kaestner KH, Grompe M. Prospective isolation of a bipotential clonogenic liver progenitor cell in adult mice. Genes Dev;25(11):1193-203. 2011 Jun 1. [PMID: 21632826] (FLOW, Mouse) FLOW Mouse
Dorrell C, Grompe MT, Pan FC, Zhong Y, Canaday PS, Shultz LD, Greiner DL, Wright CV, Streeter PR, Grompe M. Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers. Mol Cell Endocrinol;339(1-2):144-50. 2011 Jun 6. [PMID: 21539888] (FLOW, Mouse) FLOW Mouse
Lu L, Li Y, Kim SM, Bossuyt W, Liu P, Qiu Q, Wang Y, Halder G, Finegold MJ, Lee JS, Johnson RL. Hippo signaling is a potent in vivo growth and tumor suppressor pathway in the mammalian liver. Proc Natl Acad Sci U S A;107(4):1437-42. 2010 Jan 26. [PMID: 20080689] (IHC-Fr, Mouse) IHC-Fr Mouse
Dorrell, C et al. Surface markers for the murine oval cell response. Hepatology;48(4):1282-91. 2008 Oct. [PMID: 18726953] (IHC-Fr, FLOW, Mouse) IHC-Fr, FLOW Mouse

Reviews for Oval Cell Marker Antibody (NBP1-18961) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-18961:
Filter by Applications
Immunohistochemistry-Frozen
(1)
Flow Cytometry
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Oval Cell Marker NBP1-18961
Enlarge
5
reviewed by:
Simone Kurial
Immunohistochemistry-Frozen Mouse 06/04/2021
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedLiver
SpeciesMouse
Lot082718

Comments

CommentsAcetone fixation
  5
reviewed by:
Verified Customer
Flow Cytometry Mouse 12/06/2016
View

Summary

ApplicationFlow Cytometry
Sample Testedmouse liver NPC
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Oval Cell Marker Antibody (NBP1-18961). (Showing 1 - 1 of 1 FAQs).

  1. What is the recommended antigen retrieval conditions for IHC(P)?
    • The validation for IHC in paraffin embedded sections came from the following publication: NCBI site. In this publication, the authors used 3mm thick paraffin embedded tissue sections smeared on a slide fixed with methanol at 4 degrees C for 5 minutes. Therefore, we do not have a recommended antigen retrieval method. If you are trying to stain in formalin or paraformaldehyde fixed sections, I would recommend starting with either citrate buffer pH 6.0 or proteinase K for antigen retrieval.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
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Simone Kurial
06/04/2021
Application: Immunohistochemistry-Frozen
Species: Mouse

 
Verified Customer
12/06/2016
Application: Flow Cytometry
Species: Mouse