| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-687SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33122-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36561SS | |||
| Azide and BSA Free | NBP2-80818 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92687SS | |||
![Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of a mouse lymph node cryosection with rat anti-mouse CD19, clone 6D5 green in A and rat anti-mouse CD8, red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power](http://images.novusbio.com/fullsize/CD8-Antibody-YTS105-18-Immunohistochemistry-Frozen-NB200-578-img0006.jpg "Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of a mouse lymph node cryosection with rat anti-mouse CD19, clone 6D5 green in A and rat anti-mouse CD8, red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power")
![Flow Cytometry: CD8 Antibody (YTS105.18) [NB200-578] - Staining of mouse spleen cells.](http://images.novusbio.com/fullsize/CD8-Antibody-YTS105-18-Flow-Cytometry-NB200-578-img0003.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of Mouse lymph node cryosection with CD8 Antibody and HRP-conjugated goat anti-rat IgG. Low power magnification.](http://images.novusbio.com/fullsize/CD8-Antibody-YTS105-18-Immunohistochemistry-Frozen-NB200-578-img0005.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0016.jpg "Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - The antibody was used to detect RAD18 in U2os Cells. Anti-mouse 2nd antibody was used for detection (Red). DAPI was used for nuclear staining (Blue). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Immunofluorescence-H00056852-M01-img0018.jpg)
![Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Immunohistochemistry-Paraffin-H00056852-M01-img0011.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi ( Cat # H00056852-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody (M01), clone 3H7 (Cat # H00056852-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0012.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7. Analysis of RAD18 expression in PC-12.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0014.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7 Analysis of RAD18 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Immunocytochemistry-Immunofluorescence-H00056852-M01-img0009.jpg)
![ELISA: RAD18 Antibody (3H7) [H00056852-M01] - Detection limit for recombinant GST tagged RAD18 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-ELISA-H00056852-M01-img0017.jpg)
![Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Simple-Western-NB100-687-img0008.jpg "Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.")
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep13320), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0013.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Inhibition of MST improves liver regeneration in aged mice. Representative photomicrograph of an aged liver treated with siMST and stained with H&E and by IHC for CK19 and Ki67. Arrows indicate ductal regions with no signs of reaction or oval cell expansion. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunohistochemistry-NB100-687-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in MCF7 cells using Cytokeratin 19 antibody (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0004.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody [NB100-687] - An intracellular stain was performed on MCF7 cells with Cytokeratin 19 Antibody NB100-687AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Flow-Cytometry-NB100-687-img0009.jpg)
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in 1) HepG2 and 2) MCF7 lysates.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0006.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Staining of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunohistochemistry-NB100-687-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NB100-687] - TUDCA induces intermediate states of mesenchymal & epithelial cells in primary cultures of rat HSC during hepatic differentiation.Freshly isolated rat HSC (a) exhibited typical lipid droplets that contained retinoids & (b) expressed the mesenchymal filament proteins vimentin (red) & desmin (green) as investigated by immunofluorescence. (c) Under control conditions HSC remained negative for K19 expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 μM TUDCA for 14 days (red). (e–g) K19 was found to be co-expressed with desmin & vimentin (green), which indicated the origin of epithelial progenitor cells from HSC. (h–k) Also K18, Afp & Mrp2 (red), which served as markers for hepatic differentiation, were co-expressed with vimentin & desmin (green) after 21 days of TUDCA treatment. Cells with different states of maturation were still found after 21 days of TUDCA treatment. Some cells developed into hepatocyte-like cells with (j) dominant K18 filaments (white arrows) while others remained immature with persisting desmin (red arrow) or (l) K19 protein residues. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep13320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-687_rabbit-polyclonal-cytokeratin-19-antibody-31020241683916.jpg)
![Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CRM1-Antibody-Western-Blot-NB100-79802-img0026.jpg "<b>Biological Strategies Validation. </b>Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] - Association between Nup98-HoxA9 and CRM1 is critical for the Hox Gene activation mediated by Nup98-HoxA9. (B) The effect of LMB treatment on the cellular localization of Nup98-HoxA9. Nup98-HoxA9 ES cells were cultured either in the presence or absence of 5 nM LMB for 2 hr, fixed and stained with antibodies against FLAG (M2) and CRM1. Merged images of FLAG (green) and CRM1 (red) are shown. Nuclei were stained with DAPI. Bar, 10um. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/09540) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CRM1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-79802-img0028.jpg)
![Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CRM1-Antibody-Immunohistochemistry-Paraffin-NB100-79802-img0024.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CRM1 Antibody [NB100-79802] - A) Whole cell lysate from HeLa (5, 15 and 50 mcg), Ramos (50 mcg) and mouse NIH3T3 (50 mcg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. NB100-79802 used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP. CRM1 was also less efficiently immunoprecipitated by NB100-79811, which recognize upstream epitopes on CRM1.](http://images.novusbio.com/fullsize/CRM1-Antibody-Western-Blot-NB100-79802-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] - Association between Nup98-HoxA9 and CRM1 is critical for the Hox Gene activation mediated by Nup98-HoxA9. Top panel: Nup98-HoxA9 interacts and sequesters CRM1 onto Nup98-HoxA9 dots. HeLa cells were transfected with the EGFP-Nup98-HoxA9 expressing plasmid. After 24 hr, cells were fixed and stained with an anti-CRM1 antibody. Arrows indicate the cells transfected. Bottom panel: Nup98-HoxA9 ES cells were fixed and co-stained with anti-FLAG (M2) and anti-CRM1 antibodies. Merged image of FLAG (green) and CRM1 (red) is shown. Bar, 5um. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/09540) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CRM1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-79802-img0027.jpg)
![Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CRM1-Antibody-Immunohistochemistry-Paraffin-NB100-79802-img0023.jpg)
![Simple Western: CRM1 Antibody [NB100-79802] - Simple Western lane view shows a specific band for CRM1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CRM1-Antibody-Simple-Western-NB100-79802-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] - Effect of TSA treatment on the subcellular localization of Crm1.Control ES or Nup98-HoxA9 expressing ES cells were cultured in the presence or absence of 50 nM TSA for 24 hr. Then, the cells were fixed & stained with antibodies against FLAG (M2) & Crm1. Merged images of FLAG (green) & Crm1 (red) are shown. Nuclei were stained with DAPI. Bar, 10 μm. DAPI, 4',6-diamidino-2-phenylindole; ES, embryonic stem; TSA, trichostatin A.DOI://dx.doi.org/10.7554/eLife.09540.020 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/09540), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-79802_rabbit-polyclonal-crm1-antibody-310202415291981.jpg)
![Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] - A – Fluorescence images of cells transfected with thepTurboGFP-N-PON2 plasmid & then stained with anti-CRM1 antibodies. B– Fluorescence images of cells transfected with plasmidsencoding different fragments of PON2 (1–27 a.a.; 1–83 a.a.;1–168 a.a.) or GFP alone as a control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30397533), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-79802_rabbit-polyclonal-crm1-antibody-310202415304213.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-Immunohistochemistry-Frozen-NBP2-29475-img0002.jpg "Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. aCcdc88b mRNA expression in distal colons of NT (n = 3) or DSS-treated WT mice (n = 3 for each time point) at the indicated days. Data represent expression relative to hprt±SEM (n = 3). *P < 0.05, **P < 0.01 (two-tailed Student’s t-test) & are representative of one experiment. b Representative immunoblots for Ccdc88b protein detected in extracts from distal colons from NT or DSS-treated WT & Ccdc88Bmut mutant mice at indicated time points, & densitometric quantification of Ccdc88b immunoblot normalized to beta -actin±SEM (n = 3) (representative of one of two independent experiments); **P < 0.01 (two-tailed Student’s t-test). c Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at indicated times, & stained with antibodies against Ccdc88b (red), CD45 (green), & E-Cadherin (purple) & nuclei staining DAPI (blue). Scale bars, 100 μm. d Representative FACS plots & quantification of lamina propria cells stained for CD45 & for Ccdc88b antibodies for NT (n = 3) & DSS-treated WT mice (n = 4 for each time point) at indicated time points, data are representative of one experiment. e Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, & stained for Ccdc88b (red), CD3 (green) & CD11b (green); nuclei are stained with DAPI (blue). Scale bars, 100 μm. f same as in e for Ccdc88b (red), CD4 (green) & CD8 (green). Scale bars, 100 μm Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01381-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-29475_rabbit-polyclonal-cd8-antibody-imgenex-abx-160a-20ug-310202416113534.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0011.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.")
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Smooth Muscle alpha staining on E11.5 mouse Pharyngeal Mesoderm. Fixed with 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:100.Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Frozen-NBP1-30894-img0032.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel with the dilution of alpha-Smooth Muscle Actin Antibody at 1:1000. The band indicates an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0017.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0023.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0024.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0025.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0026.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat stomach. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0027.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat cervix. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0028.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Mouse duodenum. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0031.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020241959359.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis. Sample: Paraffin-embedded liver carcinoma stroma. alpha smooth muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242011442.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242075975.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242041219.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-9102024205699.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242063310.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human normal colon tissue.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-910202420122078.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-9102024205673.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-910202420104449.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242041283.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-1610202419414539.jpg)
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.](http://images.novusbio.com/fullsize/KRAS-Antibody-3B10-2F2-Western-Blot-H00003845-M01-img0004.jpg "Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.")
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/KRAS-Antibody-3B10-2F2-Western-Blot-H00003845-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of monoclonal antibody to KRAS on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/KRAS-Antibody-3B10-2F2-Immunocytochemistry-Immunofluorescence-H00003845-M01-img0006.jpg)
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - KRAS monoclonal antibody (M01), clone 3B10-2F2 Analysis of KRAS expression in HeLa.](http://images.novusbio.com/fullsize/KRAS-Antibody-3B10-2F2-Western-Blot-H00003845-M01-img0008.jpg)
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - miRNA-768-3p specifically targets K-ras. Effect of miRNA inhibitors that were reduced by co-culture on the level of pan-RAS, phospho-B-Raf and CD44 and pan-Akt by western blotting. Also shown is the effect of different miRNA inhibitors on the level of K-ras in H520 cells by western blot. Cropped blots are shown and were run under the same experimental conditions. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep02392), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KRAS-Antibody-3B10-2F2-Western-Blot-H00003845-M01-img0011.jpg)
![ELISA: KRAS Antibody (3B10-2F2) [H00003845-M01] - Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KRAS-Antibody-3B10-2F2-ELISA-H00003845-M01-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Immunocytochemistry-Immunofluorescence-NBP2-36561-img0001.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0005.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0002.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0003.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Overnight incubation with 1:50 Integrin beta 1/CD29 primary antibody in PBS/BSA 1%, at 4ºC. Green: Integrin beta 1/CD29 Antibody (P4C10), Red: Phalloidin, Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-36561_mouse-monoclonal-integrin-beta-1-cd29-antibody-p4c10-immunocytochemistry-immunofluorescence-1462023134549..jpg)
![Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Western-Blot-NB300-653-img0001.jpg "Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.")
![Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Immunohistochemistry-Frozen-NB300-653-img0006.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NB300-653-img0002.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain dilution 1:100](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NB300-653-img0003.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:00 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Rat brain-Synaptophysin.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NB300-653-img0004.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Mouse Brain- Synaptophysin.](http://images.novusbio.com/fullsize/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NB300-653-img0005.jpg)
![Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0076.jpg "Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: p53 Antibody [NB200-171] - FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p53-Antibody-Immunohistochemistry-Paraffin-NB200-171-img0075.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- anti-KLH control IgG; Red- anti-HDMX.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0002.jpg)
![Chromatin Immunoprecipitation Sequencing: p53 Antibody [NB200-171] - Chromatin from MCF7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53. ChIP-seq validation performed by Active Motif, Carlsbad, CA.](http://images.novusbio.com/fullsize/p53-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB200-171-img0073.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0003.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - Baseline p53 expression in human lymphocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: p53 Antibody [NB200-171] - Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody NB200-171 used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody NB100-2204. For blotting immunoprecipitated P53, NB200-171 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NB200-171-img0077.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Knockdown-Validated-NBP1-92687-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 1:10,000 of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP1-92687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NBP1-92687-img0003.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of different cell lysates using mouse mAb to vimentin, NBP1-92687, dilution 1:10,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, and [6] C6 cells. The band at about 50kDa mark corresponds to the vimentin protein.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Western-Blot-NBP1-92687-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [NBP1-92687] - Analysis of cortical neuron-glial cell cultures from E20 rat stained with mouse mAb to vimentin, NBP1-92687, dilution 1:2,000 in red, and costained with chicken pAb to glial fibrillary acidic protein (GFAP), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Fibroblastic and other developing cells express only vimentin and appear red. Astrocytes that express GFAP only are green while those that express both GFAP and vimentin appear golden yellow.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NBP1-92687-img0010.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (2D1) [NBP1-92687] - Human breast cancer tissue stained for Vimentin (white) and countersatined with DAPI (blue). Alexa Fluor 750 version of antibody used (NBP1-92687AF750). Image from verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunohistochemistry-Paraffin-NBP1-92687-img0012.jpg)
![Immunohistochemistry: Vimentin Antibody (2D1) [NBP1-92687] - Mouse cortex microslice section (from postnatal day 3) was immunostained with (1:500 dil.). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Immunohistochemistry-NBP1-92687-img0005.jpg)
![Simple Western: Vimentin Antibody (2D1) [NBP1-92687] - Simple Western lane view shows a specific band for Vimentin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Vimentin-Antibody-2D1-Simple-Western-NBP1-92687-img0004.jpg)
![Western Blot: Vimentin Antibody (2D1) [NBP1-92687] - High glucose (HG) concentrations induced epithelial-to-mesenchymal transition protein expression & enhanced migration activity in colorectal cancer (CRC) cells. SW480 (low metastatic potential) & SW620 (high metastatic potential) cells were cultured in different concentrations of glucose (normal: NG; HG; & osmotic control: NG + l-glucose). (A) Morphological change occurred from epithelial to mesenchymal type in the HG-concentration group. (B) HG concentration caused downregulation of E-cadherin & upregulation of N-cadherin, beta CTN, & vimentin, but c-myc was unchanged, as detected using Western blotting. beta -actin was evaluated as an internal control. (C,D) Wound healing assay showed that HG concentration promoted cell motility in SW480 & SW620 CRC cells after 48 & 72 h of culture, compared with the NG & NG + l-glucose groups. (E) In a Transwell migration assay, 3.5 × 105 SW480 & SW620 CRC cells were plated onto a 24-well plate & cultured in NG & HG-concentration medium for 96 h. HG concentration promoted cell motility in SW480 & SW620 cells. NG + l-glucose cells were evaluated as ostomic controls. (F) These data show that HG concentration caused upregulation of p-IGF1R in CRC. In addition, HG concentration promoted IGF1R downstream signaling, including p-Src & p-ERK; these proteins were increased when CRC cells were cultured in HG-concentration medium. Levels of beta -actin were evaluated as loading controls. Statistically significant differences between the two groups were judged using Student’s t-tests; * p < 0.05, ** p < 0.005, *** p < 0.001; n.s. = nonsignificant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30965609), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-92687_mouse-monoclonal-vimentin-antibody-2d1-310202416113533.jpg)
![Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat and human cells. Immunodetection of Eno1, Tpi1, Pgam1 and beta2-Tub (load control) in Poly I:C transfected and Control PaKiT03 cells at 4, 8 and 20 hpt. Image collected and cropped by CiteAb from the following publication (//www.proteomesci.com/content/13/1/25), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Triosephosphate-isomerase-Antibody-Western-Blot-NBP1-31470-img0022.jpg "Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat and human cells. Immunodetection of Eno1, Tpi1, Pgam1 and beta2-Tub (load control) in Poly I:C transfected and Control PaKiT03 cells at 4, 8 and 20 hpt. Image collected and cropped by CiteAb from the following publication (//www.proteomesci.com/content/13/1/25), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Triosephosphate isomerase Antibody [NBP1-31470] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase stained by Triosephosphate isomerase antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/Triosephosphate-isomerase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31470-img0019.jpg)
![Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NBP1-31470] - Paraffin-embedded SNU-16 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Triosephosphate-isomerase-Antibody-Immunohistochemistry-Paraffin-NBP1-31470-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Triosephosphate-isomerase-Antibody-Western-Blot-NBP1-31470-img0010.jpg)
![Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Triosephosphate-isomerase-Antibody-Western-Blot-NBP1-31470-img0016.jpg)
![Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Triosephosphate-isomerase-Antibody-Western-Blot-NBP1-31470-img0018.jpg)
![Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Triosephosphate-isomerase-Antibody-Western-Blot-NBP1-31470-img0021.jpg)
![Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat & human cells. a-d Immunodetection of Eno1, Tpi1, Pgam1 & beta 2-Tub (load control) in Poly I:C transfected & Control cells at 4, 8 & 20 hpt (a: PaKiT03, b: PaLuT02, c: HEK293T, d: HeLa). e-h Densitometry analysis following immunodetection of Eno1, Tpi1, Pgam1. Average ratios were calculated as Poly I:C/Control normalised to beta -Tub. Error bars represent the standard error of the mean from two technical replicates (e: PaKiT03, f: PaLuT02, g: HEK293T, h: HeLa) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26535029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31470_rabbit-polyclonal-triosephosphate-isomerase-antibody-310202415304217.jpg)
![Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat & human cells. a-d Immunodetection of Eno1, Tpi1, Pgam1 & beta 2-Tub (load control) in Poly I:C transfected & Control cells at 4, 8 & 20 hpt (a: PaKiT03, b: PaLuT02, c: HEK293T, d: HeLa). e-h Densitometry analysis following immunodetection of Eno1, Tpi1, Pgam1. Average ratios were calculated as Poly I:C/Control normalised to beta -Tub. Error bars represent the standard error of the mean from two technical replicates (e: PaKiT03, f: PaLuT02, g: HEK293T, h: HeLa) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26535029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31470_rabbit-polyclonal-triosephosphate-isomerase-antibody-310202415384192.jpg)
![Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat & human cells. a-d Immunodetection of Eno1, Tpi1, Pgam1 & beta 2-Tub (load control) in Poly I:C transfected & Control cells at 4, 8 & 20 hpt (a: PaKiT03, b: PaLuT02, c: HEK293T, d: HeLa). e-h Densitometry analysis following immunodetection of Eno1, Tpi1, Pgam1. Average ratios were calculated as Poly I:C/Control normalised to beta -Tub. Error bars represent the standard error of the mean from two technical replicates (e: PaKiT03, f: PaLuT02, g: HEK293T, h: HeLa) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26535029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31470_rabbit-polyclonal-triosephosphate-isomerase-antibody-310202415334932.jpg)