Oval Cell Marker Antibody (MIC1-1C3) [DyLight 550]

• Reactivity: Hu, Mu
• Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: MIC1-1C3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: DyLight 550

Oval Cell Marker Antibody (MIC1-1C3) [DyLight 550] Summary

• Immunogen: Nonparenchymal cells from DDC-treated mice
• Localization: Cell surface
• Marker: Hepatic periductal cell Marker
• Specificity: Specific for hepatic proliferating duct cells.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: This Oval Cell Marker (MIC1-1C3) antibody is useful for Immunohistochemistry on acetone fixed frozen sections and Flow cytometry. Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin were reported in scientific literature.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30236451).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Background

Facultative liver stem cell activation was originally described in the rat, where chemical damage and partial hepatectomy caused the emergence of a new cell type, the oval cell. These cells are thought to be bipotential progenitors capable of yielding both hepatocytes and bile ducts (1). To properly determine the molecular regulation of the progenitor process, appropriate markers are needed to identify and isolate cells that emerge during the oval cell response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.