| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-527SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34602-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56518SS | |||
| Azide and BSA Free | NBP2-80989 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64848SS | |||
| Azide and BSA Free | NBP2-80717 | |||
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0007.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Flow-Cytometry-NBP2-75624-img0001.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded human kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Podocin-NPHS2-Antibody-JB51-33-Immunohistochemistry-Paraffin-NBP2-75624-img0006.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Rat kidney tissue using anti-NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-immunohistochemistry-paraffin-782024103518..jpg)
![Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Analysis of NPHS2 on rat kidney tissue lysates with Rabbit anti-NPHS2 antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 42 kDaObserved band size: 50 kDaExposure time: 2 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-western-blot-782024103642..jpg)
![Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Analysis of NPHS2 on different lysates with Rabbit anti-NPHS2 antibody at 1/500 dilution.Lane 1: Human kidney tissue lysateLane 2: Mouse kidney tissue lysateLysates/proteins at 20 ug/Lane.Predicted band size: 42 kDaObserved band size: 50 kDaExposure time: 4 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-western-blot-782024103818..jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] -Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling NPHS2 and Vimentin (EM0401).The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies NPHS2 (red) at 1/100 dilution and Vimentin (green) at 1/400 dilution overnight at 4 ℃, washed with PBS.iFluor™ 594 conjugate-Goat anti-Rabbit IgG and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain](http://images.novusbio.com/fullsize/nbp2-75624_rabbit-monoclonal-podocin-nphs2-antibody-jb51-33-immunohistochemistry-paraffin-782024104013..jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0009.jpg "Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on rat brain tissue lysates with Rabbit anti-LXR alpha antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Staining LXR alpha/NR1H3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunocytochemistry-Immunofluorescence-NBP2-66938-img0002.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded mouse testes tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0005.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of HepG2 cells with LXR alpha/NR1H3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Flow-Cytometry-NBP2-66938-img0001.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha on mouse colon tissue lysate using anti-LXR alpha/NR1H3 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0006.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0007.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of LXR alpha/NR1H3 on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Western-Blot-NBP2-66938-img0008.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human placenta tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0003.jpg)
![Immunohistochemistry-Paraffin: LXR alpha/NR1H3 Antibody (JA20-38) [NBP2-66938] - Analysis of paraffin-embedded human spleen tissue using anti-LXR alpha/NR1H3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-JA20-38-Immunohistochemistry-Paraffin-NBP2-66938-img0004.jpg)
![Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Podoplanin (NZ-1.2) was detected in immersion fixed paraffin-embedded sections of human appendix using Rat Anti-Human Podoplanin Monoclonal Antibody (Catalog # NBP2-03952) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Immunohistochemistry-Paraffin-NBP2-03952-img0016.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Podoplanin (NZ-1.2) was detected in immersion fixed paraffin-embedded sections of human appendix using Rat Anti-Human Podoplanin Monoclonal Antibody (Catalog # NBP2-03952) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface.")
![Western Blot: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Detection of lysate from human A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Western-Blot-NBP2-03952-img0014.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - A surface stain was performed on Ntera2 cells with Podoplanin Antibody (NZ-1.2) NBP2-03952AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0017.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0009.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0010.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 0.2 ug of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green). Flow kit and anti-rat PE secondary were used for this test.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0012.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the PE conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.2 ug) of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0013.jpg)
![Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.](http://images.novusbio.com/fullsize/VEGF-R1-Flt-1-Antibody-Western-Blot-NB100-527-img0005.jpg "Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.")
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527] - VEGF R1/Flt-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/VEGF-R1-Flt-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-527-img0006.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells (punctate appearance typical of receptors). The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Immunohistochemistry-Paraffin-NB100-527-img0009.jpg)
![Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of a FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells. The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Immunohistochemistry-Paraffin-NB100-527-img0008.jpg)
![Western Blot: ARID1A Antibody [NB100-55334] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.1 ug/ml.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Western-Blot-NB100-55334-img0005.jpg "Western Blot: ARID1A Antibody [NB100-55334] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.1 ug/ml.")
![Chromatin Immunoprecipitation: ARID1A Antibody [NB100-55334] - In vivo Smarce1-binding sites were detected by chromatin immunoprecipitation using a SMARCE1 antibody. Cropped gel images representing typical results from ChIP assays in: the regulatory module located from the transcription initiation site +1 to -1415, which includes the basic promoter (gata5-Bp), the module located from -1469 to -1892 (gata5-A), the module located from -7823 to -8213 (gata5-B) and the module located from -16805 to -17147 (gata5-C). IgG, Smarce1, Smarca4, Arid1a and Pbrm indicate the PCR products from genomic DNA precipitated using the corresponding antibody. WT and Mut indicate the source of genomic DNA. The input is the PCR product from the total input genomic DNA. Full-length gels are presented in Supplementary Figure 3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-33746-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Chromatin-Immunoprecipitation-NB100-55334-img0006.jpg)
![Western Blot: ARID1A Antibody [NB100-55334] - Mouse embryonic fibroblasts nuclear extract at 1:1000 dilution. (Nuclear extract instead of whole cell lysate provides much better signal.) Image provided via verified customer review.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Western-Blot-NB100-55334-img0003.jpg)
![Western Blot: ARID1A Antibody [NB100-55334] - Analysis of ARID1A in zebrafish embryos using anti-ARID1A antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Western-Blot-NB100-55334-img0004.jpg)
![Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Western-Blot-NBP2-32945-img0006.jpg "Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).")
![Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Immunocytochemistry-Immunofluorescence-NBP2-32945-img0005.jpg)
![Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Flow-Cytometry-NBP2-32945-img0007.jpg)
![Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Simple-Western-NBP2-32945-img0001.jpg)
![Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 lysates(s) respectively.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-DF1513-Simple-Western-NBP2-32945-img0002.jpg)
![Western Blot: TRAIL/TNFSF10 Antibody (55B709.3) [NB100-56518] - Analysis of TRAIL in Jurkat lysate](http://images.novusbio.com/fullsize/TRAIL-TNFSF10-Antibody-55B709-3-Western-Blot-NB100-56518-img0006.jpg "Western Blot: TRAIL/TNFSF10 Antibody (55B709.3) [NB100-56518] - Analysis of TRAIL in Jurkat lysate")
![Immunohistochemistry-Paraffin: TRAIL/TNFSF10 Antibody (55B709.3) [NB100-56518] - Analysis of TRAIL in human kidney using TRAIL antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/TRAIL-TNFSF10-Antibody-55B709-3-Immunohistochemistry-Paraffin-NB100-56518-img0005.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0011.jpg "Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F9NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0010.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0007.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0009.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) [Azide Free] [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0002.jpg)
![Flow Cytometry: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Rat Splenocytes were stained with EMMPRIN/CD147 (OX-47) antibody NB100-64848 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-OX-47-Flow-Cytometry-NB100-64848-img0001.jpg "Flow Cytometry: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Rat Splenocytes were stained with EMMPRIN/CD147 (OX-47) antibody NB100-64848 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.")
![Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Analysis of FFPE tissue section of rat kidney using 1:100 dilution of CD147 antibody (clone OX-47). The staining was detected using HRP-labelled secondary antibody and DAB reagent which followed counterstaining of sections with hematoxylin. This antibody generated primarily a membrane staining with some cytoplasmic signal in the cuboidal epithelial cells of various tubules.](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-OX-47-Immunohistochemistry-Paraffin-NB100-64848-img0002.jpg)
![Western Blot: CCR10/GPR2 Antibody [NB100-56319] - Analysis for GPR2/CCR10 using NB100-56319 at 2 ug/ml dilution against 10 ug of MCF-7 (a human breast cancer cell line) cell lysate.](http://images.novusbio.com/fullsize/CCR10-GPR2-Antibody-Western-Blot-NB100-56319-img0002.jpg "Western Blot: CCR10/GPR2 Antibody [NB100-56319] - Analysis for GPR2/CCR10 using NB100-56319 at 2 ug/ml dilution against 10 ug of MCF-7 (a human breast cancer cell line) cell lysate.")
![Simple Western: CCR10/GPR2 Antibody [NB100-56319] - Simple Western lane view shows a specific band for CCR10 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CCR10-GPR2-Antibody-Simple-Western-NB100-56319-img0003.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits the ERK1/2 pathway, but not p38 pathway. Expression of Rasal1 in T cells inhibits ERK1,2 phosphorylation. Upper and lower panels: two independent experiments where primary mouse T cells were transfected with Flag-tagged Rasal1 and were ligated with anti-CD3 for 5 min followed by blotting with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204); anti-ERK1/2, anti-ZAP-70 or anti-Flag. Flag-PLK expression served as a negative control (top panel) (n > 2). Upper right panel shows endogenous levels of Rasal1 in activated primary T cells. Lower right panel: histogram showing the inhibition of pERK relative to ERK expression. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12544-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Western-Blot-NBP1-32776-img0006.jpg "Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits the ERK1/2 pathway, but not p38 pathway. Expression of Rasal1 in T cells inhibits ERK1,2 phosphorylation. Upper and lower panels: two independent experiments where primary mouse T cells were transfected with Flag-tagged Rasal1 and were ligated with anti-CD3 for 5 min followed by blotting with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204); anti-ERK1/2, anti-ZAP-70 or anti-Flag. Flag-PLK expression served as a negative control (top panel) (n > 2). Upper right panel shows endogenous levels of Rasal1 in activated primary T cells. Lower right panel: histogram showing the inhibition of pERK relative to ERK expression. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12544-4) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RASAL1 Antibody [NBP1-32776] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32776-img0003.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Western-Blot-NBP1-32776-img0004.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Protein expression was upregulated in older female crab-eating monkey brain. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RASAL1-Antibody-Western-Blot-NBP1-32776-img0005.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits in vitro anti-CD3 & dendritic cell-peptide induced T-cell activation. a Antigen (OVA323-39)-dependent activation & proliferation of DO11.10 CD4 + cells expressing Flag-tagged Rasal1 compared with control cells at different OVA peptide concentrations (5–10 ng). CTLA-Ig was used as a control. Upper inset shows expression of Flag-tagged Rasal using anti-Flag in blotting (n = 3). b Inhibition of T-cell responses by Rasal1 to increasing anti-CD3 concentrations. Upper inset: effects of Rasal1 at 48 & 72 h post-anti-CD3 activation (n = 3). c Anti-CD3-driven NFATc1/AP1 promoter activity in Jurkat T cells was inhibited by the presence of Flag-tagged Rasal1 (n = 3). d siRNA (esiRNA) knockdown of Rasal1 enhances T-cell proliferation. esiRNA were tested for their knockdown efficiency by western blot (top right panel), esiRNA1 & 2 correspond to 500 nM & 1000 nM concentrations (n = 3). e Contact time between D011.10 CD4+T-cells expressing Flag-Rasal1 (red) or empty vector (blue) & OVA peptide on bone marrow dendritic cells (BMDCs). Dot blot of T cell/DC contact duration time in presence of OVA was unaffected by Flag-tagged Rasal1 or vector transfection (n = 3). Representative plots show mean with ± SD. p-value was calculated by unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32776_rabbit-polyclonal-rasal1-antibody-310202416171418.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - TCR complex purification & Rasal1 association with ZAP-70 in the TCR complex. a Analytic gel from tandem affinity purification (TAP) of TCR plus associated proteins captured from TCR-constituted cells (TCR complex) versus TCR-null negative control cells (control) (left panel). Chart showing the list of TCR-associated proteins identified by MS analysis (right upper panel). The structure of Rasal1 (right lower panel). b Co-precipitation of ZAP-70 with TCR from 3A9 T cells. 3A9 cells were stimulated for 10 min with anti-CD3 followed by immunoprecipitation with anti-TCR zeta & blotting with anti-ZAP-70 or anti-zeta. Lane 1: resting T cells; lane 2: anti-CD3-stimulated T cells. c Rasal1 expression is induced with anti-CD3 activation. Relative fold change expression of Rasal1 versus Rasal3 upon anti-CD3 stimulation of CD4+ & CD8 + peripheral T cells. 18 s rRNA served as a control. d Anti-CD3 induces Rasal1 translocation to the membranes of T cells. Stimulation-dependent association of Rasal1 with the membrane (upper panel) versus cytosolic Rasal1 (lower panel). Anti-actin served as a loading control. LAT was used as a marker of membrane enrichment fractionation (n = 3) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32776_rabbit-polyclonal-rasal1-antibody-310202416175286.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32776_rabbit-polyclonal-rasal1-antibody-31020241618945.jpg)
![Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32776_rabbit-polyclonal-rasal1-antibody-310202416163728.jpg)
![ELISA: TMEPAI Antibody (2A12) [H00056937-M01] - Detection limit for recombinant GST tagged TMEPAI is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TMEPAI-Antibody-2A12-ELISA-H00056937-M01-img0003.jpg "ELISA: TMEPAI Antibody (2A12) [H00056937-M01] - Detection limit for recombinant GST tagged TMEPAI is approximately 1ng/ml as a capture antibody.")