Podoplanin Antibody (NZ-1.2)

Images

 
Western Blot: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Detection of lysate from human A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) ...read more
Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Podoplanin (NZ-1.2) was detected in immersion fixed paraffin-embedded sections of human appendix using Rat Anti-Human Podoplanin Monoclonal ...read more
Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - A cell surface stain was performed on A431 cells with Podoplanin (NZ-1.2) antibody NBP2-03952AF647 (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of ...read more
Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of ...read more
Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human PBMC were probed using 10 ul of CD4 antibody and 10 ...read more
Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 0.2 ug of Podoplanin antibody ...read more
Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the PE conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.2 ug) of Podoplanin antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC-Fr, IHC-P
Clone
NZ-1.2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Podoplanin Antibody (NZ-1.2) Summary

Immunogen
A synthetic peptide corresponding to amino acids 38-70 from the platelet-aggregation-stimulating (PLAG) domain of human podoplanin was used as immunogen.
Marker
Lymphatic Endothelium Marker
Isotype
IgG2a Lambda
Clonality
Monoclonal
Host
Rat
Gene
PDPN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 3-5 ug/ml
  • Flow Cytometry 0.2-1 ug/10^6 cells
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug / ml
Application Notes
IHC-P and IHC-Fr use of this product has been reported by Kaji et al 2012 (PMID: 23012488).
Control
Podoplanin Lysate (NBP2-65884)
Publications
Read Publications using
NBP2-03952 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Podoplanin Antibody (NZ-1.2)

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Background

Podoplanin is a mucin type-1 glycoprotein of 40-43kd also know as T1A, gp38 or Aggrus. Podoplanin is expressed in many tumors and many normal cells, especially lymphatic epithelial cells and follicular DCs. It appears to serve as a ligand for CLEC-2. Podoplanin expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue (Schacht, 2005, Raica, 2008, Martin-Villar, 2010). It has also been determined that podoplanin expression is induced through Src activation of Cas (Shen, 2010). Recently there are indications of podoplanin presence on Th17 cells (Bettelli, 2011). Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podoplanin Antibody (NBP2-03952)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Podoplanin Antibody (NBP2-03952) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-03952AF405
Alexa Fluor 488 Labeled NBP2-03952AF488
Alexa Fluor 647 Labeled NBP2-03952AF647
Alexa Fluor 700 Labeled NBP2-03952AF700
Allophycocyanin Labeled NBP2-03952APC
Biotin Labeled NBP2-03952B
DyLight 350 Labeled NBP2-03952UV
DyLight 405 Labeled NBP2-03952V
DyLight 488 Labeled NBP2-03952G
DyLight 550 Labeled NBP2-03952R
DyLight 650 Labeled NBP2-03952C
DyLight 680 Labeled NBP2-03952FR
DyLight 755 Labeled NBP2-03952IR
HRP Labeled NBP2-03952H
PerCP Labeled NBP2-03952PCP

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Bioinformatics Tool for Podoplanin Antibody (NBP2-03952)

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Diseases for Podoplanin Antibody (NBP2-03952)

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Research Areas for Podoplanin Antibody (NBP2-03952)

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Blogs on Podoplanin.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with...  Read full blog post.

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Bioinformatics

Gene Symbol PDPN