Podoplanin Antibody (NZ-1.2)

Western Blot: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Detection of lysate from human A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/ml.

Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Podoplanin (NZ-1.2) was detected in immersion fixed paraffin-embedded sections of human appendix using Rat Anti-Human Podoplanin Monoclonal Antibody (Catalog # NBP2-03952) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface.

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - A cell surface stain was performed on A431 cells with Podoplanin (NZ-1.2) antibody NBP2-03952AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human PBMC were probed using 10 ul of CD4 antibody and 10 ul or 0.15 ug of isotype control antibody or Podoplanin antibody.

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 0.2 ug of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green). Flow kit and anti-rat PE secondary were used for this test.

Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the PE conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.2 ug) of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green).

• Reactivity: Hu
• Applications: WB, Flow, IHC-Fr, IHC-P
• Clone: NZ-1.2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Podoplanin Antibody (NZ-1.2) Summary

• Immunogen: A synthetic peptide corresponding to amino acids 38-70 from the platelet-aggregation-stimulating (PLAG) domain of human podoplanin was used as immunogen.
• Marker: Lymphatic Endothelium Marker
• Isotype: IgG2a Lambda
• Clonality: Monoclonal
• Host: Rat
• Gene: PDPN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 3-5 ug/ml
  • Flow Cytometry 0.2-1 ug/10^6 cells
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug / ml
• Application Notes: IHC-P and IHC-Fr use of this product has been reported by Kaji et al 2012 (PMID: 23012488).
• Control:

  Podoplanin Lysate (NBP2-65884)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Podoplanin Antibody (NZ-1.2)

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Background

Podoplanin is a mucin type-1 glycoprotein of 40-43kd also know as T1A, gp38 or Aggrus. Podoplanin is expressed in many tumors and many normal cells, especially lymphatic epithelial cells and follicular DCs. It appears to serve as a ligand for CLEC-2. Podoplanin expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue (Schacht, 2005, Raica, 2008, Martin-Villar, 2010). It has also been determined that podoplanin expression is induced through Src activation of Cas (Shen, 2010). Recently there are indications of podoplanin presence on Th17 cells (Bettelli, 2011). Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podoplanin Antibody (NBP2-03952)(2)

Publications using NBP2-03952

• Cheriyan V, Wang Y, Muthu M et al. Disulfiram suppresses growth of the malignant pleural mesothelioma cells in part by inducing apoptosis. PLoS ONE 2014 Apr 02 [PMID: 24690739] (WB, Human)
  
  Details:
  H2373 malignant pleural mesothelioma cells, Fig S1B. Podoplanin migrates as multiple forms between ~50-100 kDa on WB. DSF-Cu treatment dramatically induced the 100 kDa size and overall the results suggested that DSF-Cu treatments cause post-translational
• Jamal S, Cheriyan VT, Muthu M et al. CARP-1 Functional Mimetics Are a Novel Class of Small Molecule Inhibitors of Malignant Pleural Mesothelioma Cells. PLoS ONE 2014 Mar 06 [PMID: 24598827] (WB, Human)
  
  Details:
  Malignant pleural mesothelioma H2373 cells, Fig S1. Podoplanin has extensive post-translational modifications including glycosylation and is often expressed as peptides of 50 kDa or higher, whereas its cDNA encodes for a peptide of 162 amino acids with an

Bioinformatics

• Gene Symbol: PDPN