Signal Transduction reagents: Novus Biologicals

NBP2-16258

E-Cadherin Antibody

(3 Publications)

0.1 ml

NBP2-16258

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Mouse cervix. E-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Rat intestine. E-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Breast cancer. E-cadherin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Rat prostate. E-Cadherin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - Mouse intestine. E-Cadherin stained by E-Cadherin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: E-Cadherin Antibody [NBP2-16258] - MCF-7 whole cell extract A. Control with 3 ug of preimmune Rabbit IgG B. Immunoprecipitation of E-cadherin protein by 3 ug E-cadherin antibody 5 % SDS-PAGE The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Immunoprecipitation: E-Cadherin Antibody [NBP2-16258] - Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 ug of E-Cadherin antibody. Western blot analysis was performed using E-Cadherin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog skin.E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody (NBP2-16258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody [NBP2-16258] - E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10 um.

Western Blot: E-Cadherin Antibody [NBP2-16258] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (NBP2-16258) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: E-Cadherin Antibody [NBP2-16258] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (NBP2-16258) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin protein stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.Blue: Hoechst 33342 staining.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (NBP2-16258) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. E-Cadherin antibody (NBP2-16258) diluted at 1:400.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin protein stained by E-Cadherin antibody (NBP2-16258) diluted at 1:500.Blue: Hoechst 33342 staining.

Reactivity:

Hu, Mu, Rt, Ca, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Ze

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-27202

14-3-3 Antibody - BSA Free

(1 Review)

0.1 mg

NBP2-27202

Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.

Reactivity:

Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6796A

Alt Catalog# IMG-6796A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-27202SS

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NB110-94162

MAT1/2A Antibody - BSA Free

(1 Review)

(2 Publications)

0.1 ml

NB110-94162

C1 metabolic pathway and characterization of R6/2 mice. A Folate, Met and BH4 cycles in plants and animals and their associated metabolism. Red lines stand for mammal specific, green lines stand for plant specific while black lines stand for both. All enzymes with protein levels examined by immunoblotting are marked in red. B mHtt protein aggregates in cortex and striatum regions were detected with anti-Htt antibody (mEM48) in 4-week-old male R6/2 and NCAR mice. C, D Quantification analysis of immunoblotting results of GTPCH, DHFR, QDPR, MS, MAT1/2A, AHCY, MTHFR, TPH2, TH, nNOS and ChAT (n = 7). The band intensity of each protein from western blotting D was normalized with gamma -tubulin on the same blot. The ratio was further calculated against NCAR whose relative expression level was set as 1. All data plotted are the average (n = 7) +/- SD. Only one representative western blotting of gamma -tubulin is shown. Original blots of above proteins before cropping are presented in Fig. S11. E Contents of BH4 and BH2, and their ratio in brain tissues and plasma (n = 4, average +/- SD). *p < 0.05; **p < 0.01. ***p < 0.001. Abbreviations used for enzymes: AHCYS-adenosylhomocysteine hydrolase, ChAT choline acetyltransferase, DHFR dihydrofolate reductase, GTPCH GTP cyclohydrolase I, MAT1/2A methionine adenosyltransferase, MS methionine synthase, MTHFR methylene-tetrahydrofolate reductase, nNOS neuronal nitric oxide synthase, QDPR quinoid dihydropteridine reductase, TH tyrosine hydroxylase (Tyr), TPH2 tryptophan hydroxylase Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36251090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Rt, Bv, Pm, Ze, Mu(-)

Species Glossary

Reactivity: Hu, Rt, Bv, Pm, Ze, Mu(-)

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-94162AF350
Alexa Fluor 405	NB110-94162AF405
Alexa Fluor 488	NB110-94162AF488
Alexa Fluor 532	NB110-94162AF532
Alexa Fluor 594	NB110-94162AF594
Alexa Fluor 647	NB110-94162AF647
Alexa Fluor 700	NB110-94162AF700
Alexa Fluor 750	NB110-94162AF750
Allophycocyanin	NB110-94162APC
Biotin	NB110-94162B
CoraFluor 1	NB110-94162CL1
DyLight 350	NB110-94162UV
DyLight 405	NB110-94162V
DyLight 488	NB110-94162G
DyLight 550	NB110-94162R
DyLight 594	NB110-94162DL594
DyLight 650	NB110-94162C
DyLight 680	NB110-94162FR
DyLight 755	NB110-94162IR
FITC	NB110-94162F
HRP	NB110-94162H
Janelia Fluor 525	NB110-94162JF525
Janelia Fluor 549	NB110-94162JF549
Janelia Fluor 646	NB110-94162JF646
Janelia Fluor 669	NB110-94162JF669
PE	NB110-94162PE
PerCP	NB110-94162PCP
mFluor Violet 450 SE	NB110-94162MFV450
mFluor Violet 500 SE	NB110-94162MFV500
mFluor Violet 610 SE	NB110-94162MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-94162SS

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NBP1-78964

LC3A Antibody - BSA Free

(1 Review)

0.1 ml

NBP1-78964

Immunohistochemistry: LC3A Antibody [NBP1-78964] - Analysis of LC3I in mouse testis using DAB with hematoxylin counterstain.

Reactivity:

Hu, Mu, Rt, Po, Bv, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78964AF350
Alexa Fluor 405	NBP1-78964AF405
Alexa Fluor 488	NBP1-78964AF488
Alexa Fluor 532	NBP1-78964AF532
Alexa Fluor 594	NBP1-78964AF594
Alexa Fluor 647	NBP1-78964AF647
Alexa Fluor 700	NBP1-78964AF700
Alexa Fluor 750	NBP1-78964AF750
Allophycocyanin	NBP1-78964APC
Biotin	NBP1-78964B
CoraFluor 1	NBP1-78964CL1
DyLight 350	NBP1-78964UV
DyLight 405	NBP1-78964V
DyLight 488	NBP1-78964G
DyLight 550	NBP1-78964R
DyLight 594	NBP1-78964DL594
DyLight 650	NBP1-78964C
DyLight 680	NBP1-78964FR
DyLight 755	NBP1-78964IR
FITC	NBP1-78964F
HRP	NBP1-78964H
Janelia Fluor 525	NBP1-78964JF525
Janelia Fluor 549	NBP1-78964JF549
Janelia Fluor 646	NBP1-78964JF646
Janelia Fluor 669	NBP1-78964JF669
PE	NBP1-78964PE
PerCP	NBP1-78964PCP
mFluor Violet 450 SE	NBP1-78964MFV450
mFluor Violet 500 SE	NBP1-78964MFV500
mFluor Violet 610 SE	NBP1-78964MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-78964SS

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H00026330-M01

GAPDH-2 Antibody (2E3-2E10) - Azide and BSA Free

(12 Publications)

0.1 mg

H00026330-M01

Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu, Ec

Species Glossary

Reactivity: Hu, Ec

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-89011

Rhot1 Antibody - BSA Free

(9 Publications)

0.1 ml

NBP1-89011

Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human cerebral cortex shows positivity in neuronal cells.

Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human liver shows positivity in hepatocytes.

Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human pancreas shows positivity in exocrine glandular cells.

Reactivity:

Hu, Rt, Mu

Species Glossary

Reactivity: Hu, Rt, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89011-25ul

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NBP1-85799

$Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 RNA mislocalization does not affect RAB13 binding to membranes or association with REP‐1 or RabGDICells treated with the indicated PMOs were fractionated into soluble & particulate fractions, & the indicated proteins were detected by Western blot. RhoGDI & TfRc serve as soluble & particulate markers, respectively.Lysates from the indicated GFP or GFP‐RAB13‐expressing cell lines were immunoprecipitated with anti‐GFP antibodies & blotted to detect the indicated proteins. Relative REP‐1 & RabGDI binding are quantified in the graphs from n = 3 (REP‐1) & n = 5 (RabGDI) independent experiments. Bars: mean ± s.e.m. No significant differences were detected by Wilcoxon matched‐pairs signed‐rank test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 RNA & protein exhibit distinct subcellular distributionsRepresentative FISH images showing RAB13 RNA distribution in MDA‐MB-231 cells. Nuclei & cell outlines are shown in blue & green, respectively. Arrows point to RAB13 RNA concentrated at protrusive regions. Boxed regions are magnified in the insets.Representative immunofluorescence images of RAB13 protein in cells transfected with the indicated siRNAs. Reduction of intensity in RAB13 knockdown cells confirms the specificity of the signal. Arrows point to perinuclear RAB13 protein. Calibration bar shows intensity values.Ratios of peripheral/perinuclear intensity calculated from images as shown in (A) & (B). Bars: mean ± s.e.m. Values within each bar represent number of cells observed in 3 independent experiments.Protrusions (Ps) & cell bodies (CB) of cells induced to migrate toward LPA were isolated & analyzed to detect the indicated proteins (by Western blot; left panels) or RNAs (by RT‐ddPCR; right panel). Ps/CB enrichment ratios from 2 independent experiments are shown. Bars: mean ± s.e.m. The enrichment of pY397‐FAK serves to verify the enrichment of protrusions containing newly formed adhesions in the Ps fraction.Data information: P‐values: **< 0.01; ****< 0.0001 by Student's t‐test (C) or analysis of variance with Dunnett's multiple comparisons test against GFP (D). Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: Rab13 Antibody [NBP1-85799] - Peripheral RAB13 RNA translation is required for RAB13 protein activation but not steady‐state distribution or membrane associationAWide‐field images of RAB13 immunofluorescence in MDA‐MB-231 cells treated with control or RAB13 (191 + 230) PMOs & ratios of peripheral/perinuclear intensity. Scale bars: 10 μm. n = 45–50 cells. Bars: mean ± s.e.m. Similar results were observed in two additional independent experiments.BFluorescence images (projections of confocal slices throughout the cell height) of cells expressing GFP‐RAB13 with the indicated UTRs. Note that in both cases the protein assumes indistinguishable distribution. Scale bars: 10 μm.CSoluble/particulate fractionation of the indicated cell lines followed by Western blot to detect the indicated proteins. RhoGDI & TfRc serve as soluble & particulate markers, respectively. Graph shows quantitation from n = 3 independent experiments. Bars: mean ± s.e.m.D, EActive RAB13 (RAB13‐GTP) was pulled down using MICAL‐L1 RBD‐GST from the indicated PMO‐treated cells (D) or GFP‐RAB13‐expressing lines (E). The amount of endogenous or exogenous RAB13 was measured by quantitative Western blot, & relative levels of active RAB13 are plotted. n = 8 (D), n = 4 (E). Bars: mean ± s.e.m.Data information: P‐values: *< 0.05, **< 0.01 by Kruskal–Wallis test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Immunocytochemistry/ Immunofluorescence: Rab13 Antibody [NBP1-85799] - RAB13 protein levels do not change upon serum stimulationMDA‐MB‐231 cells were stimulated with serum for the indicated times, in the presence or absence of cycloheximide (CHX). The cells were also treated with control or RAB13‐mislocalizing PMOs.Representative Western blot analysis of whole‐cell lysates & corresponding quantitations of RAB13 levels from n = 4–5 replicates. Bars: mean ± s.e.m. No significant differences by Friedman's test. Increase in pY397‐FAK levels attests to serum stimulation.RAB13 immunofluorescence at representative protrusive regions. A cell line expressing GFP was used to delineate cell borders & provide an internal cytosolic control. RAB13 signal at front lamellipodial regions was quantified. n = 35–51 protrusions. Bars: mean ± s.e.m. No increase is detected upon stimulation. By contrast, at early time points a decrease is detected (5 & 20 min, P < 0.01 by Kruskal–Wallis test), potentially arising from serum‐induced endocytosis of RAB13‐containing membranes. Scale bars: 8 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 protein levels do not change upon serum stimulationMDA‐MB‐231 cells were stimulated with serum for the indicated times, in the presence or absence of cycloheximide (CHX). The cells were also treated with control or RAB13‐mislocalizing PMOs.Representative Western blot analysis of whole‐cell lysates & corresponding quantitations of RAB13 levels from n = 4–5 replicates. Bars: mean ± s.e.m. No significant differences by Friedman's test. Increase in pY397‐FAK levels attests to serum stimulation.RAB13 immunofluorescence at representative protrusive regions. A cell line expressing GFP was used to delineate cell borders & provide an internal cytosolic control. RAB13 signal at front lamellipodial regions was quantified. n = 35–51 protrusions. Bars: mean ± s.e.m. No increase is detected upon stimulation. By contrast, at early time points a decrease is detected (5 & 20 min, P < 0.01 by Kruskal–Wallis test), potentially arising from serum‐induced endocytosis of RAB13‐containing membranes. Scale bars: 8 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

<b>Genetic Strategies Validation. </b>Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB13 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85799-25ul

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NBP1-47833

MEK1 Antibody (OTI1F5)

(1 Review)

(1 Publication)

0.1 ml

NBP1-47833

Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded prostate tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded colon tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded endometrium tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Ovary tissue using anti-MEK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded pancreas tissue using anti-MEK1mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-72643AF350
Alexa Fluor 405	NBP2-72643AF405
Alexa Fluor 488	NBP2-72643AF488
Alexa Fluor 532	NBP2-72643AF532
Alexa Fluor 594	NBP2-72643AF594
Alexa Fluor 647	NBP2-72643AF647
Alexa Fluor 700	NBP2-72643AF700
Alexa Fluor 750	NBP2-72643AF750
Allophycocyanin	NBP2-72643APC
Biotin	NBP2-72643B
CoraFluor 1	NBP2-72643CL1
DyLight 350	NBP2-72643UV
DyLight 405	NBP2-72643V
DyLight 488	NBP2-72643G
DyLight 550	NBP2-72643R
DyLight 594	NBP2-72643DL594
DyLight 650	NBP2-72643C
DyLight 680	NBP2-72643FR
DyLight 755	NBP2-72643IR
FITC	NBP2-72643F
HRP	NBP2-72643H
Janelia Fluor 525	NBP2-72643JF525
Janelia Fluor 549	NBP2-72643JF549
Janelia Fluor 646	NBP2-72643JF646
Janelia Fluor 669	NBP2-72643JF669
PE	NBP2-72643PE
PerCP	NBP2-72643PCP
mFluor Violet 450 SE	NBP2-72643MFV450
mFluor Violet 500 SE	NBP2-72643MFV500
mFluor Violet 610 SE	NBP2-72643MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-72643

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NB100-1953

Phosphoserine Antibody - BSA Free

(1 Review)

(6 Publications)

0.4 ml

NB100-1953

Western Blot: Phosphoserine Antibody [NB100-1953] - ~30 ug SY5Y or N2A whole cell lysate separated on 8% PAGE, blotted and probed with phosphoserine antibody (1:125 in 3% BSA in TBST). Western blot image submitted by a verified customer review.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-1953-100ul

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NB120-11512

E-Cadherin Antibody (DECMA-1)

(2 Reviews)

(3 Publications)

0.025 ml

NB120-11512

Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Cat testis, cat ovary and HEK293 whole cell lysates. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:3200 Rat Monoclonal Anti-Uvomorulin/E-Cadherin, Clone: DECMA-1. Detection using Goat Anti-Rat IgG, FITC conjugate.

Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 diluted to 1:3200. The antibody was developed using Rabbit Anti-Rat IgG, FITC-conjugate.

Reactivity:

Hu, Mu, Bv, Ca, Fe

Species Glossary

Reactivity: Hu, Mu, Bv, Ca, Fe

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-67501

TOMM20 Antibody (ST04-72)

(5 Publications)

100 ul

NBP2-67501

Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of Hela cells with TOMM20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (ST04-72) [NBP2-67501] - Staining TOMM20 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (ST04-72) [NBP2-67501] - Staining TOMM20 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human liver cancer tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded mouse heart tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded mouse kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded mouse small intestine tissue using anti-TOMM20 antibody. Counter stained with hematoxylin. The nuclear counter stain is DAPI (blue).

Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: TOMM20 Antibody (ST04-72) [NBP2-67501] - Analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-TOMM20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western blot: Rabbit Monoclonal TOMM20 Antibody (ST04-72) [NBP2-67501] - TOMM20 identification in different MSC cell lysates. No signal was obtained in extracellular vesicles. A secondary fluorescent antibody anti-rabbit was used. Image from a verified customer review.

Western Blot: Rabbit Monoclonal TOMM20 Antibody (ST04-72) [NBP2-67501] - TOMM20 identification in rat astrocytes cell lysate. No signal was obtained in extracellular vesicles. A secondary fluorescent antibody anti-rabbit was used. The membrane was stripped and re-probed again. Image from a verified customer review.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-67471

c-jun [p Ser63] Antibody (SY0297)

(1 Review)

(1 Publication)

100 ul

NBP2-67471

Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human endometrial tissue with Rabbit anti-c-jun [p Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T

Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Western blot analysis of c-jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Human U251 glioma cell line, whole cell lysates. Cells have been treated with JNK and p38 inhibitors. WB image submited by a verified customer review.

Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of c-Jun (p Ser63) on different lysates with Rabbit anti-c-Jun (p Ser63) antibody at 1/500 dilution. Lane 1: U87MG cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-c-jun [Ser63] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Inhibitor Family

Inhibitor Target

Isotype

Kit Type

Lysate Subcellular Fraction

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

E-Cadherin Antibody

14-3-3 Antibody - BSA Free

MAT1/2A Antibody - BSA Free

LC3A Antibody - BSA Free

GAPDH-2 Antibody (2E3-2E10) - Azide and BSA Free

Rhot1 Antibody - BSA Free

Rab13 Antibody - BSA Free

MEK1 Antibody (OTI1F5)

Phosphoserine Antibody - BSA Free

E-Cadherin Antibody (DECMA-1)

TOMM20 Antibody (ST04-72)

c-jun [p Ser63] Antibody (SY0297)