| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-39002SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19371SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27216 | |||
| Azide and BSA Free | NBP2-25199SS | |||
| Azide and BSA Free | NBP2-27216-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-26585 | |||
| BSA Free | NBP2-25191SS | |||
| Azide and BSA Free | NBP2-26585-1mg | |||
| Azide and BSA Free | NBP2-26585-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56582SS | |||
| Azide and BSA Free | NBP2-33297 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64988SS | |||
| Azide and BSA Free | NBP2-80647 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77109-0.025mg | |||
.png "CD4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse CD4, Monoclonal Antibody (Catalog# NBP3-14741) at 1:250 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=")
DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse CD4, Monoclonal Antibody (Catalog# NBP3-14741) at 1:250 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2181SS | |||
| Azide and BSA Free | NBP2-80646 | |||
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB100-39002-img0011.jpg "Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.")
![Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on SK-MEL-28 cells with NB100-39002 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Flow-Cytometry-NB100-39002-img0022.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB100-39002-img0017.jpg)
![Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-NB100-39002-img0014.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - FFPE bovine spleen tissue. Deparaffinization and antigen retrieval was carried out with PTlink from Dako, citrate buffer pH 6. Anti-Foxp3 antibody was used at a 1:100 dilution. Secondary reagent: Envision, from Dako. Staining: DAB and hematoxylin. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB100-39002-img0024.jpg)
![Western Blot: FoxP3 Antibody [NB100-39002] - Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and analyzed by Western blot with anti-Foxp3 antiserum (1:2000 dilution).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Western-Blot-NB100-39002-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: FoxP3 Antibody [NB100-39002] - Transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-39002-img0012.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counterstained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB100-39002-img0015.jpg)
![Flow Cytometry: FoxP3 Antibody [NB100-39002] - Transfectants were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of positively stained cells are shown.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Flow-Cytometry-NB100-39002-img0013.jpg)
![Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Flow-Cytometry-NB100-39002-img0021.jpg)
![Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD8A mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD8A and FoxP3 antibody at 1:400 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Dual-RNAscope-ISH-IHC-NB100-39002-img0028.jpg)
![Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD4 mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD4 and FOXP3 antibody at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/FoxP3-Antibody-Dual-RNAscope-ISH-IHC-NB100-39002-img0029.jpg)
![Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody - BSA Free [NB100-39002] - CCR10-expressing CD4+CD25+FOXP3+ Treg cells are enriched in the spinal cord after SCI. (A, B) Representative flow cytometry dot plots (A) & percentage (B) of CD4+CD25+FOXP3+ cells among the CD4+ T cells in the spinal cord after sham or SCI surgery. Cells were gated on 7-AAD negative population & the numbers inside plots refer to % Treg cells (n=5). (C, D) Representative histogram of CCR10 (C) & CCR3 (D) expression in CD4+CD25+FOXP3+ cells as gated in (A). (E) CD4+CD25+FOXP3+ cells in the spinal cord from sham & SCI mice were sorted out & analyzed by Western blotting to detect the expression of CCR10 & CCR3. GAPDH was used as a loading control. (F) Double immunostaining of FOXP3 (red) & CCR10 (green) in the spinal cord sections after sham or SCI surgery. The merged images are also shown. Arrows indicate cells with positive staining & colocalization. Scale bar, 100 μm. Data are mean ± SD. The statistical analysis was performed using Student’s t-test. **, P<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31557129), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-39002_rabbit-polyclonal-foxp3-antibody-310202415541911.jpg)
![Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody - BSA Free [NB100-39002] - Effects of anaphylatoxins on transcription factor phosphorylation in ARPE-19 cells. (A–C) Protein phosphorylation of (A) CREB (Ser133); (B) FoxP3 (Ser418); & (C) FOXO1 (Ser256) was examined in ARPE-19 cells treated with C3a, C5a, or the combination of C3a/C5a at 1, 5, 10, & 15 min. Phosphorylation levels were normalized to beta -actin (relative p-protein levels), & slopes of the time course were determined. Anaphylatoxins were applied at concentrations of 300 nM (C3a) & 50 nM (C5a). Data are mean + SEM, n = 3, *p < 0.05, ***p < 0.001 (Student’s t-test). (D) Immunofluorescence staining of FoxP3. Upper panel: Lewis rat (albino) eye with experimental uveitis; lower panel: normal rat eye. Asterisks mark immune cells infiltrating the retina from the choroid through the retinal pigment epithelium (RPE) in the eye with uveitis (clinical score of 2, histological score of 1). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2017.00703/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-39002_rabbit-polyclonal-foxp3-antibody-31020241554547.jpg)
![Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-NBP1-19371-img0017.jpg "Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.")
![Immunohistochemistry: CD4 Antibody [NBP1-19371] - DBZ inhibits the accumulation of CD4+ T cells and Th2 differentiation in the AAAs. (B) The representation of immunohistochemical staining for CD4+ in abdominal aorta from four groups (left). Bar graphs show the percentage of CD4+ positive cell areas (right; n=3 per group). Bar: 50 um.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-NBP1-19371-img0018.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/fullsize/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19371-img0005.jpg)
![Flow Cytometry: CD4 Antibody - BSA Free [NBP1-19371] - An intracellular stain was performed on U-251 MG cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/CD4-Antibody---BSA-Free-Flow-Cytometry-NBP1-19371-img0023.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - Mouse spleen, 25ug total protein. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-Western-Blot-NBP1-19371-img0007.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Staining of paraffin-embedded mouse brain tissue. Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0006.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE tissue section of mouse brain using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody primarily generated a strong signal in the membranes of a subset of cells in the tested section.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0011.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong signal in the membranes of a subset of cells in the spleen section and the staining showed punctate appearance in some cells.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0012.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-Frozen-NBP1-19371-img0013.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/CD4-Antibody-Flow-Cytometry-NBP1-19371-img0015.jpg)
![Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD4-Antibody-Flow-Cytometry-NBP1-19371-img0016.jpg)
![Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/CD4-Antibody-Flow-Cytometry-NBP1-19371-img0021.jpg)
![Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/CD4-Antibody-Flow-Cytometry-NBP1-19371-img0022.jpg)
![Simple Western: CD4 Antibody [NBP1-19371] - Lane view shows a specific band for CD4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CD4-Antibody-Simple-Western-NBP1-19371-img0009.jpg)
![Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - IFNG mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-IFNG and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin (Cat. No. DS9800), BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/CD4-Antibody-Dual-RNAscope-ISH-IHC-NBP1-19371-img0019.jpg)
![Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - CCR5 mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CCR5 and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/CD4-Antibody-Dual-RNAscope-ISH-IHC-NBP1-19371-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: CD4 Antibody - BSA Free [NBP1-19371] - Rats with mild liver damage show immune cells infiltration in cerebellum at 4 weeks, which is prevented by rifaximin. Analysis of immune cells infiltration in cerebellum was performed at 4 weeks by immunohistochemistry using antibodies against (A) CD4, a marker of T lymphocytes (F(3,8) = 4.511, p < 0.01) & (B) IBA1, a marker of meningeal & perivascular macrophages (K-W(4,10) = 27.76, p < 0.0001). (C) Double immunofluorescence with anti-CD4 & anti-CX3CR1 as markers of autoreactive CD4+CD28− T lymphocytes was performed & quantified (K-W(4,5) = 8.711, p < 0.05). (D) Double immunofluorescence with anti-CD4 & anti-CCR6 as marker of Th17 lymphocytes was performed & quantified (K-W(4,7) = 8.858, p < 0.05). Number of animals in each group was added under group names. One-way ANOVA with Tukey’s test (CD4+) & nonparametric Kruskal–Wallis (K-W statistic) with Dunn’s test (Iba1, CD4+/CX3CR1+ & CD4+/CCR6+) was performed to compare all groups. Values significantly different from control rats are indicated by asterisks & from CCl4 rats by a. * p < 0.05, a p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19371_rabbit-polyclonal-cd4-antibody-310202416163759.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0007.jpg "Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.")
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in human PBMC using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0004.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the FITC conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0011.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the DyLight 405 conjugate of NBP2-27216. Staining of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0005.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of this antibody. Green represents isotype control; red represents anti-CD4 antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0009.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Allophycocyanin conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0010.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) - Azide and BSA Free [NBP2-25199] - analysis of CD4 in human PBMCs using 0.1 ug of this antibody. Secondary antibody is goat anti-mouse PE.](http://images.novusbio.com/fullsize/nbp2-25199_mouse-monoclonal-cd4-antibody-rpa-t4-imgenex-img-5916a-25ug-17720241044654.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody (GK1.5) [NBP2-25191] - CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Clone GK1.5) at 1 ug/mL overnight at 4 degrees C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes.](http://images.novusbio.com/fullsize/CD4-Antibody-GK1-5-Immunohistochemistry-Frozen-NBP2-25191-img0005.jpg "Immunohistochemistry-Frozen: CD4 Antibody (GK1.5) [NBP2-25191] - CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Clone GK1.5) at 1 ug/mL overnight at 4 degrees C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes.")
![Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the PE conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of this antibody ; red represents mouse CD4 this antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-GK1-5-Flow-Cytometry-NBP2-25191-img0008.jpg)
![Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the Allophycocyanin conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents mouse CD4 this antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-GK1-5-Flow-Cytometry-NBP2-25191-img0006.jpg)
![Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the FITC conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of NBP2-26584. Green represents isotype control (20212C); red represents mouse CD4 NBP2-26584 antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-GK1-5-Flow-Cytometry-NBP2-25191-img0007.jpg)
![Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/mL.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Western-Blot-NB100-56582-img0018.jpg "Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/mL.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody. 1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0010.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody, 5 ug/10^6 cells. Products used: anti-CD4 PE conjugate, anti-CD25 APC conjugate, anti-FOXP3 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0013.jpg)
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of 2 x 10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0012.jpg)
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti-CD25 APC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0017.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.1 ug/10^6 cells. Anti-CD25 FITC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0014.jpg)
![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Flow-Cytometry-NB100-64988-img0008.jpg "Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.")
![Flow (Cell Surface): CD4 Antibody (W3/25) [NB100-64988] - Rat Splenocytes were stained with NB100-64988 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alex Fluor 488.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Flow-Cell-Surface-NB100-64988-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody (W3/25) [NB100-64988] - Staining of rat splenocytes with CD4 (W3/25) antibody. Live cells were incubated with CD4 antibody on ice, then fixed with 2% formaldehyde prior to secondary antibody incubation.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Immunocytochemistry-Immunofluorescence-NB100-64988-img0007.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody (W3/25) [NB100-64988] - Staining of frozen rat spleen with Mouse anti Rat CD4.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Immunohistochemistry-Frozen-NB100-64988-img0001.jpg)
![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Staining of stimulated rat spleen cells.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Flow-Cytometry-NB100-64988-img0003.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419592087.jpg "Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Mouse Testis.Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202420312999.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Human Testis.Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Nanog antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241932686.jpg)
![Immunocytochemistry/ Immunofluorescence: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in HeLa Cells.Immunofluorescent analysis of PFA-fixed HeLa cells labeling Nanog with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419553033.jpg)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in Mouse and Rat Testis.Antibodies: Nanog, 2 u/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241923639.gif)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in HeLa CellsLoading: 15 u of lysate. Antibodies: Nanog, 2 u/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419463442.gif)
![Immunoprecipitation: CD4 Antibody (BL-155-1C11) [NBP2-76423] - Detection of human CD4 by WB of immunoprecipitates from MOLT-4 lysate. Antibodies: Rabbit anti-CD4 recombinant monoclonal [BL-155-1C11] (NBP2-76423) and another rabbit anti-CD4 antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-BL-155-1C11-Immunoprecipitation-NBP2-76423-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: CD4 Antibody (BL-155-1C11) [NBP2-76423] - Detection of human CD4 by WB of immunoprecipitates from MOLT-4 lysate. Antibodies: Rabbit anti-CD4 recombinant monoclonal [BL-155-1C11] (NBP2-76423) and another rabbit anti-CD4 antibody.")
![<b>Independent Antibodies Validation. </b>Western Blot: CD4 Antibody (BL-155-1C11) [NBP2-76423] - Whole cell lysate (10 ug) from U-937, HEK293T, MOLT-4, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody [BL-155-1C11] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/nbp2-76423_rabbit-monoclonal-cd4-antibody-bl-155-1c11-western-blot-272202465128..jpg)
![Flow Cytometry- CD4 Antibody (BL-155-1C11) [NBP2-76423] -Rabbit anti-CD4 recombinantmonoclonal [BL-155-1C11] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76423_rabbit-monoclonal-cd4-antibody-bl-155-1c11-flow-cytometry-27220246544..jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BL-155-1C11) [NBP2-76423] -Rabbit anti-CD4 recombinant monoclonal antibody[BL-155-1C11]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp2-76423_rabbit-monoclonal-cd4-antibody-bl-155-1c11-immunohistochemistry-paraffin-272202465557..jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - (5ug/ml) staining of paraffin embedded Mouse Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NB600-243_Immunohistochemistry-Paraffin-FoxP3-Antibody-NB600-243-5ug-ml-staining-of-paraffin-embedded-Mouse-Spleen-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254016.jpg "Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - (5ug/ml) staining of paraffin embedded Mouse Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Flow Cytometry: FoxP3 Antibody [NB600-243] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Flow-Cytometry-NB600-243-img0003.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB600-243] - Negative Control showing staining of paraffin embedded Mouse Spleen, with no primary antibody.](http://images.novusbio.com/fullsize/NB600-243_Immunohistochemistry-Paraffin-FoxP3-Antibody-NB600-243-Negative-Control-showing-staining-of-paraffin-embedded-Mouse-Spleen-with-no-primary-antibody--29320239254029.jpg)
![Western Blot: CD4 Antibody (BLR167J) [NBP3-14741] - Whole cell lysate (10 ug) from NIH 3T3, BW5147.3, mouseThymus, 70Z/3, and EL4 cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-CD4 recombinant monoclonalantibody [BLR167J] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgGChemiluminescence with an exposure timeof 30 seconds. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Western-Blot-NBP3-14741-img0003.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse lung, : Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Immunohistochemistry-Paraffin-NBP3-14741-img0006.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse spleen, Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Immunohistochemistry-Paraffin-NBP3-14741-img0004.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse gut, Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Immunohistochemistry-Paraffin-NBP3-14741-img0005.jpg)
![Immunoprecipitation: CD4 Antibody [NBP3-14741] - s: Whole cell lysate (200 ugper IP reaction; 10% of IP loaded) from mouse thymus.Antibodies: Rabbit anti-CD4 recombinant monoclonalantibody used for IP at 6 ul/200ug lysate. CD4 was also immunoprecipitated by anotherantibody against a different epitope of CD4 For blotting immunoprecipitated CD4, was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunoprecipitation-NBP3-14741-img0001.jpg)
![Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB110-40660-img0002.jpg "Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB110-40660] - Staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Antibody at 5 ug/mL. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40660-img0001.jpg)
![Flow Cytometry: CD4 Antibody (B486A1) [NB100-2181] - Analysis of blood T cells using CD4 mouse mAb (M2) and negative control (M1).](http://images.novusbio.com/fullsize/CD4-Antibody-B486A1-Flow-Cytometry-NB100-2181-img0004.jpg "Flow Cytometry: CD4 Antibody (B486A1) [NB100-2181] - Analysis of blood T cells using CD4 mouse mAb (M2) and negative control (M1).")
![Western Blot: CD4 Antibody (B486A1) [NB100-2181] - Analysis of human tonsil tissue using CD4 antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/CD4-Antibody-B486A1-Western-Blot-NB100-2181-img0005.jpg)
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