| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78444SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-111SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19167SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05767SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33130 | |||
| IHC-Prediluted | NBP2-45339 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1596SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-90592SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2688SS | |||
| Azide and BSA Free | NBP2-80873 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89113-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-125SS | |||
![Western Blot: Mas Antibody [NBP1-78444] - Analysis of MAS1 in: (1) Ntera 2, (2) A431, (3) HepG2, (4) MCF7, (5) 3T3, and (6) Cos7.](http://images.novusbio.com/fullsize/Mas-Antibody-Western-Blot-NBP1-78444-img0008.jpg "Western Blot: Mas Antibody [NBP1-78444] - Analysis of MAS1 in: (1) Ntera 2, (2) A431, (3) HepG2, (4) MCF7, (5) 3T3, and (6) Cos7.")
![Immunocytochemistry/Immunofluorescence: Mas Antibody [NBP1-78444] - Tested at 1:25 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Mas-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78444-img0006.jpg)
![Immunohistochemistry: Mas Antibody [NBP1-78444] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Mas-Antibody-Immunohistochemistry-NBP1-78444-img0007.jpg)
![Simple Western: Mas Antibody [NBP1-78444] - Lane view shows a specific band for MAS1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mas-Antibody-Simple-Western-NBP1-78444-img0009.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT decreases p53mut stability. T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 i1/4M), Nutlin3A (5 i1/4M) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget (2014)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-111-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT decreases p53mut stability. T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 i1/4M), Nutlin3A (5 i1/4M) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget (2014)) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT modulates p53 stability in-vivo and synergizes with ionizing radiation to inhibit tumor growth( Sections of PSN1 xenografts treated with three consecutive doses of MK-2206 (60 mg/kg). Sections of PSN1 xenografts and in-vitro PSN1 cells fixed and stained with anti-NPM (red) and anti-p14ARF (green). Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget (2014)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunocytochemistry-Immunofluorescence-NB200-111-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: p14ARF/CDKN2A Antibody [NB200-111] - p14ARF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue). Tubulin was stained with alpha tubulin (red).](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunocytochemistry-Immunofluorescence-NB200-111-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p14ARF/CDKN2A Antibody [NB200-111] - ARF expression levels in OCI/AML3 cells after proteasome treatment. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-111-img0009.jpg)
![Flow Cytometry: p14ARF/CDKN2A Antibody [NB200-111] - p14ARF antibody was tested at 1:400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Flow-Cytometry-NB200-111-img0007.jpg)
![Western Blot: p14ARF/CDKN2A Antibody [NB200-111] - Analysis of HeLa Whole Cell Lysate (NB800-PC1) using p14ARF antibody (lot C) employing ECL detection method.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Western-Blot-NB200-111-img0008.jpg)
![Immunohistochemistry-Frozen: p14ARF/CDKN2A Antibody [NB200-111] - Mouse brain section, hippocampal (10 um thickness). 3% PFA perfused. 1:1000 dilultion. Image from verified customer review. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/p14ARF-CDKN2A-Antibody-Immunohistochemistry-Frozen-NB200-111-img0010.jpg)
![Western Blot: p14ARF/CDKN2A Antibody - BSA Free [NB200-111] - Inhibition of AKT decreases p53mut stability(A) T24 cells & PSN1 cells were treated with MK-2206 (5 μM) or DMSO for 24 hrs. Cells were fixed & stained with DAPI & anti-mutant p53 (OP29 clone). (B) T24 cells were pre-treated with MK-2206 (5 μM) or DMSO for 24 hrs before the addition of fresh media containing cyclohexamide (100 μM) (CHX) in combination with MK-2206 (5 μM) or DMSO for the times indicated. Nuclear extracts were prepared from treated cells & blotted with the indicated antibodies. Quantification is relative to initiation of CHX treatment for both conditions *, MK-2206 is 40% of DMSO control but taken as 1.0 for relative assessment. (C) H1299 cells were transfected with HA-tagged-ubiquitin & mutant p53 (R175H or R248W) as indicated. Transfected cells were treated with DMSO, MK-2206 (5 μM) or Nutlin3A (5 μM) for 16hrs as indicated. p53 immunoprecipitates & whole cell lysates were probed with the indicated antibodies. (D) T24 cells were transfected with non-targeting control or p14ARF siRNA & treated with DMSO, MK-2206 (5 μM) or the NPM oligomerisation inhibitor NCS348884 (4 μM) (Qi et al., 2008) as indicated. (E) T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 μM), Nutlin3A (5 μM) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. (F) KPC mice derived KRASG12D p53 Floxed (p53Fl), KRASG12D p53R172H ARF+/+ & KRASG12D p53R172H ARF−/− pancreatic tumor cells were treated with MK-2206 (1μM), Nutlin3A (5μM) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25071014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-111_rabbit-polyclonal-p14arf-cdkn2a-antibody-310202416203719.jpg)
![Western Blot: p14ARF/CDKN2A Antibody - BSA Free [NB200-111] - Inhibition of AKT decreases p53mut stability(A) T24 cells & PSN1 cells were treated with MK-2206 (5 μM) or DMSO for 24 hrs. Cells were fixed & stained with DAPI & anti-mutant p53 (OP29 clone). (B) T24 cells were pre-treated with MK-2206 (5 μM) or DMSO for 24 hrs before the addition of fresh media containing cyclohexamide (100 μM) (CHX) in combination with MK-2206 (5 μM) or DMSO for the times indicated. Nuclear extracts were prepared from treated cells & blotted with the indicated antibodies. Quantification is relative to initiation of CHX treatment for both conditions *, MK-2206 is 40% of DMSO control but taken as 1.0 for relative assessment. (C) H1299 cells were transfected with HA-tagged-ubiquitin & mutant p53 (R175H or R248W) as indicated. Transfected cells were treated with DMSO, MK-2206 (5 μM) or Nutlin3A (5 μM) for 16hrs as indicated. p53 immunoprecipitates & whole cell lysates were probed with the indicated antibodies. (D) T24 cells were transfected with non-targeting control or p14ARF siRNA & treated with DMSO, MK-2206 (5 μM) or the NPM oligomerisation inhibitor NCS348884 (4 μM) (Qi et al., 2008) as indicated. (E) T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 μM), Nutlin3A (5 μM) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. (F) KPC mice derived KRASG12D p53 Floxed (p53Fl), KRASG12D p53R172H ARF+/+ & KRASG12D p53R172H ARF−/− pancreatic tumor cells were treated with MK-2206 (1μM), Nutlin3A (5μM) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25071014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-111_rabbit-polyclonal-p14arf-cdkn2a-antibody-310202416212372.jpg)
![Immunocytochemistry/ Immunofluorescence: p14ARF/CDKN2A Antibody - BSA Free [NB200-111] - Inhibition of AKT modulates p53 stability in-vivo & synergizes with ionizing radiation to inhibit tumor growth(A) PSN1 xenografts (PSN1 cells co-injected with LTC-14 stellate cells) established in the flank of athymic nude mice were treated with MK-2206 (60 mg/kg-320 mg/kg) as indicated or beta -cyclo-dextrin (1.5 mg/ml) carrier. Xenograft tumors were lysed & lysates probed by western blot with the indicated antibodies. (B-D) Sections of PSN1 xenografts treated with three consecutive doses of MK-2206 (60 mg/kg). (B) Sections of PSN1 xenografts & in-vitro PSN1 cells fixed & stained with anti-NPM (red) & anti-p14ARF (green). (C) PSN1 xenografts treated with MK-2206 or carrier were stained with DAPI, anti-p53 (DO1) or p53mut (OP29 clone) (D) PSN1 xenografts treated with MK-2206 (60 mg/kg) or carrier were stained by immunohistochemical methods with anti-pS473-AKT, anti-phospho-S48-NPM (pS48-NPM) or p53. (E) PSN1 xenografts established in the flank of athymic nude mice were injected subcutaneously with two alternate day doses of MK-2206 (60 mg/kg) or carrier. Mice were subsequently treated with a single dose of IR (6 Gy) & tumor volumes measured regularly with callipers. Dash lines indicate tumor growth differential at 250 mm3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25071014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-111_rabbit-polyclonal-p14arf-cdkn2a-antibody-310202416203724.jpg)
![Western Blot: p14ARF/CDKN2A Antibody - BSA Free [NB200-111] - Inhibition of AKT decreases p53mut stability(A) T24 cells & PSN1 cells were treated with MK-2206 (5 μM) or DMSO for 24 hrs. Cells were fixed & stained with DAPI & anti-mutant p53 (OP29 clone). (B) T24 cells were pre-treated with MK-2206 (5 μM) or DMSO for 24 hrs before the addition of fresh media containing cyclohexamide (100 μM) (CHX) in combination with MK-2206 (5 μM) or DMSO for the times indicated. Nuclear extracts were prepared from treated cells & blotted with the indicated antibodies. Quantification is relative to initiation of CHX treatment for both conditions *, MK-2206 is 40% of DMSO control but taken as 1.0 for relative assessment. (C) H1299 cells were transfected with HA-tagged-ubiquitin & mutant p53 (R175H or R248W) as indicated. Transfected cells were treated with DMSO, MK-2206 (5 μM) or Nutlin3A (5 μM) for 16hrs as indicated. p53 immunoprecipitates & whole cell lysates were probed with the indicated antibodies. (D) T24 cells were transfected with non-targeting control or p14ARF siRNA & treated with DMSO, MK-2206 (5 μM) or the NPM oligomerisation inhibitor NCS348884 (4 μM) (Qi et al., 2008) as indicated. (E) T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 μM), Nutlin3A (5 μM) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. (F) KPC mice derived KRASG12D p53 Floxed (p53Fl), KRASG12D p53R172H ARF+/+ & KRASG12D p53R172H ARF−/− pancreatic tumor cells were treated with MK-2206 (1μM), Nutlin3A (5μM) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25071014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-111_rabbit-polyclonal-p14arf-cdkn2a-antibody-310202416205197.jpg)
![Western Blot: p14ARF/CDKN2A Antibody - BSA Free [NB200-111] - Inhibition of AKT promotes enhanced MDM2 activity via the increased association between NPM & p14ARF(A) Npm−/−, p53−/−double null MEF were infected with pBABE retrovirus empty vector & pBABE expressing FLAG-tagged-NPM-WT, NPM-S48A or S48E as indicated. Immunopurification of NPM was done by pulling down with the Flag tag (middle panel) followed by elution of complexes by the Flag peptide & subsequent immunopurification of endogenous MDM2 (lower panel). (B) Nuclear immunoprecipitates of MDM2 from T24 cells treated with MK-2206 (5 μM, 24 hrs). Immunoprecipitates & lysates were blotted with the indicated antibodies. (C) T24 cells were treated with MK-2206 (5 μM) as indicated. p14ARF was immunoprecipitated from whole cell lysates & nuclear extracts & the association with NPM & MDM2 determined by western blot. Immunoprecipitates & lysates were blotted with the indicated antibodies. (D) MDM2 & (E) p53 ubiquinitation assay in H1299 cells transfected with wild type p53, HA-tagged ubiquitin & treated for 16 hrs with DMSO, MK-2206 (5 μM) or Nutlin3A (5 μM) as indicated. Immunoprecipitates & whole cell lysates were probed with the indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25071014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-111_rabbit-polyclonal-p14arf-cdkn2a-antibody-310202416212370.jpg)
![Western Blot: p14ARF/CDKN2A Antibody - BSA Free [NB200-111] - Inhibition of AKT promotes enhanced MDM2 activity via the increased association between NPM & p14ARF(A) Npm−/−, p53−/−double null MEF were infected with pBABE retrovirus empty vector & pBABE expressing FLAG-tagged-NPM-WT, NPM-S48A or S48E as indicated. Immunopurification of NPM was done by pulling down with the Flag tag (middle panel) followed by elution of complexes by the Flag peptide & subsequent immunopurification of endogenous MDM2 (lower panel). (B) Nuclear immunoprecipitates of MDM2 from T24 cells treated with MK-2206 (5 μM, 24 hrs). Immunoprecipitates & lysates were blotted with the indicated antibodies. (C) T24 cells were treated with MK-2206 (5 μM) as indicated. p14ARF was immunoprecipitated from whole cell lysates & nuclear extracts & the association with NPM & MDM2 determined by western blot. Immunoprecipitates & lysates were blotted with the indicated antibodies. (D) MDM2 & (E) p53 ubiquinitation assay in H1299 cells transfected with wild type p53, HA-tagged ubiquitin & treated for 16 hrs with DMSO, MK-2206 (5 μM) or Nutlin3A (5 μM) as indicated. Immunoprecipitates & whole cell lysates were probed with the indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25071014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-111_rabbit-polyclonal-p14arf-cdkn2a-antibody-310202416205120.jpg)
![Western Blot: LC3A Antibody [NBP1-19167] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-LC3A in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection LC3 II upon chloroquine treatment.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP1-19167-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: LC3A Antibody [NBP1-19167] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-LC3A in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection LC3 II upon chloroquine treatment.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3A Antibody [NBP1-19167] - LC3/MAP1 [NBP1-19167] - LC3 antibody was tested in HeLa cells with Dylight 488 (green). Cells were treated overnight with 50 uM chloroquine to induce autophagosome formation. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19167-img0013.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP1-19167] - Analysis of a FFPE tissue section of mouse brain using LC3 antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the sections/nuclei were further counterstained with hematoxylin. Note the diffused cytoplasmic staining of LC3 in all of the cells with highest positivity in various neurons.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP1-19167-img0014.jpg)
![Western Blot: LC3/MAP1LC3A Antibody [NBP1-19167] - Human brain lysate.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Western-Blot-NBP1-19167-img0004.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP1-19167] - Analysis of a FFPE tissue section of mouse liver using LC3 antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the sections/nuclei were further counterstained with hematoxylin. Note the diffused cytoplasmic staining of LC3 in all of the hepatocytes and other liver cells.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Immunohistochemistry-Paraffin-NBP1-19167-img0010.jpg)
![Simple Western: LC3A Antibody [NBP1-19167] - Image shows a specific band for LC3 in 0.5 mg/mL of Neuro2A lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Simple-Western-NBP1-19167-img0009.jpg)
![Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP1-05767-img0002.jpg "Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.")
![Flow Cytometry: DIO3 Antibody [NBP1-05767] - Analysis using the Biotin conjugate of NBP1-05767. Dio3 staining of mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.](http://images.novusbio.com/fullsize/DIO3-Antibody-Flow-Cytometry-NBP1-05767-img0005.jpg)
![Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP1-05767-img0003.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Western-Blot-NB600-1160-img0013.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Immunocytochemistry-Immunofluorescence-NB600-1160-img0012.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining seen in cytoplasm, ER and membrane. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Specimens which have not been fixed in acetic acid/alcohol require pre-treatment with citrate buffer: for example, unmask of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Immunohistochemistry-Paraffin-NB600-1160-img0004.jpg)
![Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (31A3)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Flow-Cytometry-NB600-1160-img0011.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of UchL1 in 1) human, 2) mouse and 3) rat brain lysate using UchL1 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Western-Blot-NB600-1160-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of PGP9.5 in rat mesenteric artery using anti-PGP9.5 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Immunocytochemistry-Immunofluorescence-NB600-1160-img0005.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Simple-Western-NB600-1160-img0006.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Electropherogram images of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Simple-Western-NB600-1160-img0010.jpg)
![Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0005.jpg "Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1596-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0006.jpg)
![Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0003.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of hypothyroid (Hypo; solid bar; n = 6) rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of hypothyroid ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 100 um (1st image); and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0007.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Aromatase expression by Western blotting (WES system) from in vitro activated M1 and M2 rat macrophages using Aromatase antibody. Controls include ovary (positive control) and ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) and without (negative control) forskolin treatment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29898754/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase Antibody-Simple Western-NB100-1596-img0009.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241539251.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415392577.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415363733.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415293319.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415382447.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Metformin decreases aromatase-positive, tumor-associated macrophages. a Quantification of aromatase-positive cells & b CD68-positive (CD68+) macrophages costaining for aromatase in the tumor border of tissues from control or metformin-treated rats. c Representative control & metformin-treated IHC images of CD68 & aromatase dual staining in tissue bordering mammary tumors (T) (arrows: yellow = CD68+aromatase+, brown = CD68+; scale bar = 50um). d Aromatase expression by Western blotting (WES system) from in vitro activated M1 & M2 rat macrophages using two different primary antibodies (Ab #1: Novus NB100-1596; Ab #2: clone 677, Baylor College of Medicine). Controls include ovary (positive control) & ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) & without (negative control) forskolin treatment. e IHC intensity score for ER & PR (0 = no stain; 1 = weak; 2 = moderate; 3 = strong staining). *p < 0.05 Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-0974-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241682139.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58872] - Analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, NB110-58872, dilution 1:2,000 in green, and mouse mAb to Actin, dilution 1:1,000, in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties but not in lysates of HeLa, NIH-3T3 and other non-neuronal cells. Actin is detected with apparent molecular weight of 42 kDa and provides an excellent loading control.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NB110-58872-img0002.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58872] - Analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, NB110-58872, dilution 1:2,000 in green, and mouse mAb to Actin, dilution 1:1,000, in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties but not in lysates of HeLa, NIH-3T3 and other non-neuronal cells. Actin is detected with apparent molecular weight of 42 kDa and provides an excellent loading control.")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - (A) Immunolabeling of the neuronal marker UCHL1 and TRPV1 in frozen DRG sections and cultured sensory neurons from rat. TRPV1 is selectively expressed in a subpopulation of sensory neurons. (B) Representative view field showing the automated image analysis to quantify TRPV1 expression in cultured sensory neurons. Green encircled objects represent sensory neurons marked by UCHL1. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0115731), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58872-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1, NB110-58872 , dilution 1:500 in red, and costained with mouse mAb to vimentin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58872-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-310202416212311.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - PGD2 is a paracrine mediator synthesized in myelinated large-diameter neurons acts on TRPV1(+) neurons.(A) Dose-dependent induction of RII phosphorylation in sensory neurons after 1 min stimulation w/ PGD2 (EC50 = 377 nM, n = 3,>2000 neurons/condition; one-way ANOVA w/ Bonferroni's multiple comparisons test). (B) PGD2 did not induce pRII in non-neuronal cells of same cultures shown in A. (C) Time course of RII phosphorylation indicating long-lasting effects of PGD2 (10 µM) on sensory neurons. (D) Stimulation w/ PGD2 also results in phosphorylation of ERK1/2 measured in same cultures shown in D. (E) Representative experiment demonstrating induction of RII phosphorylation is enhanced in TRPV1(+) neurons (total of 3664 neurons). Plots of respective controls are shown in S2 Fig. (F) Fold changes of pRII intensities in TRPV1(−) (grey bars) & TRPV1(+) (black bars) neurons after 1 min stimulation w/ 10 µM PGD2 (n = 3,>2000 neurons/condition, one-way ANOVA w/ Bonferroni's multiple comparisons test). (G) Co-labeling of TRPV1 & PTGDS revealing PTGDS is expressed in neurons lacking TRPV1 (total of 9951 neurons, also refer to S2 Fig. for control plots). (H) Co-labeling of NF200 & PTGDS showing PTGDS(+) neurons express NF200 (total of 12966 neurons, also refer to S2 Fig. for control plots).(I) Size distribution of PTGDS(+) (green), NF200(+) (red), & all sensory neurons (black) indicating PTGDS(+) neurons are larger than other neurons. (J) Suggested pathway of interneuronal communication between subgroups of sensory neurons. Large-diameter mechanosensitive neurons express PTGDS resulting in production of PGD2, which activates DP1 receptors present on nociceptive neurons expressing TRPV1. Image collected & cropped by CiteAb from following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-31020241621833.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-31020241620379.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-310202416235521.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-31020241623555.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-3102024162498.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-310202416235515.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB110-90592 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NB110-90592-img0008.jpg "Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB110-90592 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB110-89717 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NB110-90592-img0009.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Deregulated MYC transforms breast acini into invasive breast carcinoma in vivo. Tumours harvested in Figure 1C were stained (above) for Ki67/MKI67 and TUNEL and were quantified (below) for the degree of Ki67/MKI67 and TUNEL staining. Individual quantifications per tumour are shown; *P<=0.05, ***P<=0.001, one-way ANOVA with Bonferroni post-test for multiple testing. Scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.038083), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-NB110-90592-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-90592] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Ki-67/MKI67 (NB110-90592) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Chromatin-Immunoprecipitation-NB110-90592-img0003.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Detection of human lymph node. (20X)](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-NB110-90592-img0004.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody [NB110-90592] - Detection of human lymph node. (40X)](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-NB110-90592-img0005.jpg)
![Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human NG2/MCSP (LHM2) Polyclonal Antibody (Catalog # NB100-2688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for NG2/MCSP (LHM2) at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-LHM-2-Knockout-Validated-NB100-2688-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human NG2/MCSP (LHM2) Polyclonal Antibody (Catalog # NB100-2688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for NG2/MCSP (LHM2) at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Analysis of NG2 expression in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-LHM-2-Western-Blot-NB100-2688-img0006.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Staining of NG2 in human brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-LHM-2-Immunohistochemistry-Paraffin-NB100-2688-img0005.jpg)
![Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on SK-MEL-28 cells with NG2/MCSP antibody (LHM 2) NB100-2688AF700 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-LHM-2-Flow-Cytometry-NB100-2688-img0014.jpg)
![Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-LHM-2-Flow-Cytometry-NB100-2688-img0004.jpg)
![Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-LHM-2-Flow-Cell-Surface-NB100-2688-img0009.jpg)
![Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-LHM-2-Flow-Cell-Surface-NB100-2688-img0010.jpg)
![Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-LHM-2-Flow-Cell-Surface-NB100-2688-img0013.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining in human endometrium and liver tissues . Corresponding FUS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0027.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining in human endometrium and liver tissues . Corresponding FUS RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: FUS Antibody [NBP1-89113] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-FUS antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NBP1-89113-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody [NBP1-89113] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89113-img0028.jpg)
![Western Blot: FUS Antibody [NBP1-89113] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NBP1-89113-img0011.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human cerebellum shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0022.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0023.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human endometrium shows strong nuclear positivity in glandular cells and stromal cells.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0024.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human liver shows moderate nuclear positivity in bile duct cells while hepatocytes are negative.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0025.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NBP1-89113] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NBP1-89113-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - HeLa cells. Primary antibody at 1:100, secondary antibody: goat-anti mouse IgG AlexaFluor 568 at 1:100. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-2A7-A1-Immunocytochemistry-Immunofluorescence-NB300-581-img0010.jpg "Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - HeLa cells. Primary antibody at 1:100, secondary antibody: goat-anti mouse IgG AlexaFluor 568 at 1:100. ICC/IF image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-2A7-A1-Immunohistochemistry-Paraffin-NB300-581-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-2A7-A1-Immunocytochemistry-Immunofluorescence-NB300-581-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-2A7-A1-Immunocytochemistry-Immunofluorescence-NB300-581-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-2A7-A1-Immunocytochemistry-Immunofluorescence-NB300-581-img0006.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-2A7-A1-Immunohistochemistry-Paraffin-NB300-581-img0007.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human liver tissues.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-2A7-A1-Immunohistochemistry-Paraffin-NB300-581-img0009.jpg)
![Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NB100-125-img0003.jpg "Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.")
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-125-img0005.jpg)
![Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NB100-125-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-125-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-125-img0004.jpg)
