| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49536SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-537-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19775SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-412SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-125SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47470SS | |||
| Azide and BSA Free | NBP2-80757 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47339SS | |||
| Azide and BSA Free | NBP2-80748 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61576SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-102SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-07047SS | |||
![Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NBP1-49536) at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49536-img0010.jpg "Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NBP1-49536) at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - Analysis of Pyruvate Carboxylase in A. Human liver extracts and B. Mouse liver extracts](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP1-49536-img0007.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP1-49536] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse kidney using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a diffused cytoplasmic staining in all the tubules and a subset of cells in glomerular cells.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-49536-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - Characterization of MPC1-/- in the RM-1 murine prostate cancer cells. Western blotting results of subcellular proteins from WT and MPC1-/- cells and corresponding densitometry histograms of ALT1 and Pyruvate Carboxylase. Cyto means cytoplasmic protein. Mito stands for mitochondrial protein. Protein alpha-Tublin was used as a loading control. ***P<0.001. All experiments were performed at least three times with consistent and repeatable results. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP1-49536-img0012.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP1-49536] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse adipose tissue using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a specific staining of the fat cells in the tested section.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-49536-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Pyruvate Carboxylase at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49536-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. Divergent effects of human cytomegalovirus and herpes simplex virus-1 on cellular metabolism. PLoS Pathog (2011)](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Knockdown-Validated-NBP1-49536-img0011.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0008.jpg "Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Western-Blot-NB300-537-img0006.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Hek293 cells with PPAR alpha/NR1C1 [3B6/PPAR] Antibody NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0009.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Western-Blot-NB300-537-img0002.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0003.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0004.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0005.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free [NB300-537] - PPAR alpha/NR1C1 was knocked down with siRNA in primary cultured rat cardiomyocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-BSA-Free-Knockdown-Validated-NB300-537-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Analysis of IL1 beta Antibody in extracts from HeLa cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-19775-img0004.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Analysis of IL1 beta Antibody in extracts from HeLa cells.")
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody - BSA Free [NBP1-19775] - In situ expression of IL-6 in glomerular and tubulointerstitial compartments in patients with diabetic nephropathy DN (right) and control group (left). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32723296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody---BSA-Free-Immunohistochemistry-NBP1-19775-img0006.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Staining in the cytoplasm of villi and in the crypt cells of human lung carcinoma tissue.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-19775-img0005.jpg)
![Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Western-Blot-NB600-412-img0007.jpg "Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.")
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - HeLa cells, cultured on cover slips, were fixed with 10% formalin for 10 minutes and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were then incubated with 1:200 dilution of anti-58K Golgi Protein antibody (clone 58K-9) for overnight at 4C and detected with an anti-mouse Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) [NBP2-31156]. Cells were imaged using a 40X objective. Antibody clone 58K-9 generated a specific signal in the Golgi apparatus of the cells.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunocytochemistry-Immunofluorescence-NB600-412-img0010.jpg)
![Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - DMD muscle co-stained for Neu5Gc (green) with 58K Golgi, a Golgi marker, in red, and DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc and 58K Golgi. Bar is 50 um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0088226) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunohistochemistry-NB600-412-img0011.jpg)
![Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K Golgi Protein in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunohistochemistry-NB600-412-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Assessing Golgi morphology observed apoptosis in mBECs using Mouse 58K Golgi Protein at a dilution of 1:100 overnight at 4 °C. This image was taken on a Zeiss LSM 710 Confocal Microscope (META) with Zeiss Plan Apo 63x/1.40 oil. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb600-412_mouse-monoclonal-58k-golgi-protein-antibody-58k-9-immunocytochemistry-immunofluorescence-204202310243.jpg)
![Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Co-localization of Neu5Gc staining with markers for endosomes & Golgi in BMD & DMD muscle.(A) BMD muscle co-stained for Neu5Gc (green) & clathrin (red), a marker of endosomes. Merged image on right shows overlap of Neu5Gc & clathrin expression in yellow. Arrow marks several examples of co-staining. (B) DMD muscle co-stained for Neu5Gc (green) with LAMP1, a lysosomal marker, 58K Golgi, a Golgi marker, or calnexin, & endoplasmic reticulum marker, all in red, & DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc & 58K Golgi. Bar is 50 µm for all panels in A & B. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24505439), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-412_mouse-monoclonal-58k-golgi-protein-antibody-58k-9-310202415304219.jpg)
![Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0010.jpg "Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]")
![<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types. Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Western-Blot-NB100-902HIF2-img0012.jpg)
![Simple Western: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Simple-Western-NB100-902HIF2-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - HIF-1alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia. EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. Non-infected cells and GFP infected cells served as controls. Immunoblot analyses of EMSCs derived from the indicated genotypes treated with 0.1 mM DMOG for 24 hrs. Attenuated WNT11 expression in Hif-1alpha KO EMSCs (lenti-Cre infected Hif-1af/f). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Knockout-Validated-NB100-902HIF2-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Formalin-fixed paraffin-embedded tissue sections of human placenta were probed for HIF-2 alpha/EPAS1 mRNA (ACD RNAScope Probe, catalog #410598; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB100-122) at 1:100 dilution with one-hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to trophoblastic cells. HIF-2 alpha/EPAS1 Antibody [NB100-122]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0014.jpg)
![Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Normal ganglia 1 (NG1), normal ganglia 2 (NG2) and IDH-mutant (IDH). Sporadic PGL (Spo. PGL). HIF2 alpha staining. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0127471) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunocytochemistry-Immunofluorescence-NB300-511-img0006.jpg "Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.")
![Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Normal biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunohistochemistry-Paraffin-NB300-511-img0003.jpg)
![Flow Cytometry: CFTR Antibody (CF3) [NB300-511] - Human airway epithelial cells stained with CFTR (CF3) coupled with goat anti-mouse FITC conjugated secondary antibody. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Flow-Cytometry-NB300-511-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - Staining of human pancreas cryosection (200x). Alpha cells are strongly labeled. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunocytochemistry-Immunofluorescence-NB300-511-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunocytochemistry-Immunofluorescence-NB300-511-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunocytochemistry-Immunofluorescence-NB300-511-img0005.jpg)
![Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Cancer biopsies of deparaffinized Human colon carcinoma tissue.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunohistochemistry-Paraffin-NB300-511-img0001.jpg)
![Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Normal biopsies of deparaffinized Human pancreas tissue.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunohistochemistry-Paraffin-NB300-511-img0002.jpg)
![Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NB100-125-img0003.jpg "Western Blot: PER2 Antibody [NB100-125] - Western Blot Usage of NB100-125. Image submitted via verified customer review.")
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-125-img0005.jpg)
![Western Blot: PER2 Antibody [NB100-125] - WB analysis of PER2 expression on ARPE-19 whole cell lysate.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NB100-125-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - The PER2 antibody was tested in ARPE-19 cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-125-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PER2 Antibody [NB100-125] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PER2 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PER2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-125-img0004.jpg)
![Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Western-Blot-NBP1-47470-img0012.jpg "Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunocytochemistry-Immunofluorescence-NBP1-47470-img0006.jpg)
![Immunohistochemistry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas and GSK-3 beta in a murine xenograft model. SW620 cells injected into flanks of athymic Nude mice & tumor volume was checked biweekly. Mice treated weekly with oxaliplatin (days 0, 7 and 14) & injected daily with Fap1 blocking SLV peptide, VLS control peptide, or treated with SLV or VLS peptide alone (n=12). Tumors were simultaneously harvested when control tumors were >2,000 mm3. SLV peptide increases Gsk3-phosphorylation with/without oxaliplatin. IF detection of total versus phospho- GSK-3 beta performed with DAPI staining of nuclei (areas without gland formation were selected). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.25401), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunohistochemistry-NBP1-47470-img0014.jpg)
![Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - An intracellular stain was performed on HeLa cells with GSK-3 beta (3D10) antibody NBP1-47470PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Flow-Cytometry-NBP1-47470-img0013.jpg)
![Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Western-Blot-NBP1-47470-img0008.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunohistochemistry-Paraffin-NBP1-47470-img0007.jpg)
![Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Flow-Cytometry-NBP1-47470-img0005.jpg)
![Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Simple-Western-NBP1-47470-img0009.jpg)
![Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Simple-Western-NBP1-47470-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases Fas & Gsk3 beta phosphorylation in CD133+ cells in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases Fas phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Fas or CD133 was performed with DAPI staining of nuclei. (B) SLV peptide increases Gsk3 beta phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Gsk3 beta or CD133 was performed with DAPI staining of nuclei. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47470_mouse-monoclonal-gsk-3-beta-antibody-3d10-310202416171462.jpg)
![Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas & Gsk3 beta in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases gland formation in xenograft tumors with or without oxaliplatin. Histology was analyzed by hematoxylin/ eosin staining. Fap1 expression was determined by immunofluorescence. Relative fluorescent intensity (RFI) of Fap1 staining is indicated below relevant panels. (B) SLV peptide increases Fas-phosphorylation in xenograft tumors with or without by oxaliplatin. Immunofluorescent detection of total versus phospho-Fas was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. (C) SLV peptide increases Gsk3 beta -phosphorylation with or without oxaliplatin. Immunofluorescent detection of total versus phospho- Gsk3 beta was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47470_mouse-monoclonal-gsk-3-beta-antibody-3d10-3102024161604.jpg)
![Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Western-Blot-NBP1-47339-img0006.jpg "Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of methanol-fixed HepG2 (left) and HeLa (right) cells using anti-GAPDH mAb (green), showing cytoplasmic localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0004.jpg)
![Flow Cytometry: GAPDH Antibody (1A10) [NBP1-47339] - An intracellular stain was performed on U-87 cells with GAPDH [1A10] Antibody NBP1-47339AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Flow-Cytometry-NBP1-47339-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 2 ug/mL of mouse anti-GAPDH monoclonal (NB300-328), 2 ug/mL mL of mouse anti- GAPDH monoclonal (NBP1-47339). Cells were stained using the appropriate secondary antibody donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0008.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunohistochemistry-Paraffin-NBP1-47339-img0005.jpg)
![Simple Western: GAPDH Antibody (1A10) [NBP1-47339] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Simple-Western-NBP1-47339-img0007.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Knockdown-Validated-NB110-61576-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0004.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Generation and validation of NOX4 knockout (KO) HeLa cell lines. NOX4 knockout did not influence NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 levels. Protein levels of NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 in parental HeLa cells and three clones of NOX4 knockout cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0170327), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0003.jpg)
![Western Blot: SR-BII Antibody [NB400-102] - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate](http://images.novusbio.com/fullsize/SR-BII-Antibody-Western-Blot-NB400-102-img0002.jpg "Western Blot: SR-BII Antibody [NB400-102] - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate")
![Immunocytochemistry/Immunofluorescence: SR-BII Antibody [NB400-102] - SR-BII antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/SR-BII-Antibody-Immunocytochemistry-Immunofluorescence-NB400-102-img0003.jpg)
![Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0002.jpg "Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.")
![Western Blot: PDK4 Antibody [NBP1-07047] - Mouse liver. NuPAGE 4-12%. This image was submitted via customer review.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0003.jpg)
![Western Blot: PDK4 Antibody [NBP1-07047] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. * = vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0004.jpg)
![Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Genetic manipulations can alter PDH E1 alpha phosphorylation in response to hypoxia.(a) MIA PaCa-2 control (Hg), silenced HIF1 alpha (shHIF1 alpha ) or silenced PDHK1 (shPDHK1) & PDP1 overexpressing cells were incubated for 16 h in normoxia or hypoxia (0.5% O2) & phospho-serine E1 alpha detected by Western blot showing that all three modifications can have an inhibitory effect on hypoxic Ser232 phosphorylation. (b) Increased pSer232-E1 alpha detection after E1 alpha immunoprecipitation from lysates of cells treated as in (a). (c) The same analysis as in (a) after treatment with 16 h 1 mM DMOG. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07047_rabbit-polyclonal-pdk4-antibody-31020241534333.jpg)
![Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Hypoxia inhibits mitochondrial OCR & PDH activity & induces PDHK1 protein & activity.(a) Ratio of oxygen consumption rate (OCR) to extracellular acidification rate (ECAR) measured by Seahorse XF in MIA PaCa-2, PANC-1, & SU.86.86 cell lines in high (25 mM) or low (0.5 mM) glucose incubated overnight with or without 1 mM DMOG. (mean ± SEM, two-tailed Student’s t-test, **p < 0.01, ***p < 0.001) (b) Cell-based PDH activity assay in cells incubated 16 h in normoxia, hypoxia (0.5% O2) or 1 mM DMOG. (mean ± SD, one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001) (c) Western blots of HIF alpha isoforms, pyruvate dehydrogenase kinase isoforms (PDHKs), phosphatase (PDP1 – lower band *), target phosphorylated serine residues on E1 alpha & total E1 alpha after overnight incubation in normoxia or hypoxia (0.5% O2) at 25 or 5 mM glucose as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07047_rabbit-polyclonal-pdk4-antibody-310202416171443.jpg)