GSK-3 beta Antibody (3D10) - Azide and BSA Free

Images

 
Western Blot: GSK-3 beta Antibody (3D10) - Azide and BSA Free [NBP2-80757] - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates. Image from the standard ...read more
Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - Azide and BSA Free [NBP2-80757] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA ...read more
Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) - Azide and BSA Free [NBP2-80757] - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB ...read more
Flow Cytometry: GSK-3 beta Antibody (3D10) - Azide and BSA Free [NBP2-80757] - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple). Image from the standard format of ...read more
Western Blot: GSK-3 beta Antibody (3D10) - Azide and BSA Free [NBP2-80757] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review. Image from the ...read more
Flow Cytometry: GSK-3 beta Antibody (3D10) - Azide and BSA Free [NBP2-80757] - An intracellular stain was performed on HeLa cells with GSK-3 beta (3D10) antibody NBP1-47470PE (blue) and a matched isotype control ...read more
Simple Western: GSK-3 beta Antibody (3D10) - Azide and BSA Free [NBP2-80757] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing ...read more
Simple Western: GSK-3 beta Antibody (3D10) - Azide and BSA Free [NBP2-80757] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
3D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

GSK-3 beta Antibody (3D10) - Azide and BSA Free Summary

Immunogen
Purified recombinant fragment of human GSK3 beta expressed in E. coli. [UniProt# P49841]
Specificity
Human, mouse, rat and monkey.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GSK3B
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot : 1:500-1:2000
  • Simple Western : 1:50
  • ELISA : 1:10000
  • Flow Cytometry : 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry : 1:200-1:1000
  • Immunohistochemistry-Paraffin
  • CyTOF-ready
Application Notes
This GSK3 beta (3D10) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for GSK-3 beta Antibody (3D10) - Azide and BSA Free

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

GSK3 (Glycogen synthase kinase 3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK3 are ubiquitously expressed, although particularly high levels of GSK 3 beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK3 alpha and Ser9 of GSK3 beta, S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GSK-3 beta Antibody (NBP2-80757) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-47470AF350
Alexa Fluor 405 NBP1-47470AF405
Alexa Fluor 488 NBP1-47470AF488
Alexa Fluor 532 NBP1-47470AF532
Alexa Fluor 594 NBP1-47470AF594
Alexa Fluor 647 NBP1-47470AF647
Alexa Fluor 700 NBP1-47470AF700
Alexa Fluor 750 NBP1-47470AF750
Allophycocyanin NBP1-47470APC
Allophycocyanin/Cy7 NBP1-47470APCCY7
Biotin NBP1-47470B
DyLight 350 NBP1-47470UV
DyLight 405 NBP1-47470V
DyLight 488 NBP1-47470G
DyLight 550 NBP1-47470R
DyLight 594 NBP1-47470DL594
DyLight 650 NBP1-47470C
DyLight 680 NBP1-47470FR
DyLight 755 NBP1-47470IR
FITC NBP1-47470F
HRP NBP1-47470H
Janelia Fluor 549 NBP1-47470JF549
Janelia Fluor 646 NBP1-47470JF646
PE NBP1-47470PE
PE/Atto594 NBP1-47470PEATT594
PE/Cy5.5 NBP1-47470PECY55
PE/Cy7 NBP1-47470PECY7
PerCP NBP1-47470PCP

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Bioinformatics

Gene Symbol GSK3B