CFTR Antibody (CF3)

Images

 
Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed ...read more
Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Normal biopsies of deparaffinized Human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - Staining of human pancreas cryosection (200x). Alpha cells are strongly labeled. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed ...read more
Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed ...read more
Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Cancer biopsies of deparaffinized Human colon carcinoma tissue.
Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Normal biopsies of deparaffinized Human pancreas tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, B/N, Flow, ICC/IF, IHC, IHC-P, IP
Clone
CF3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CFTR Antibody (CF3) Summary

Immunogen
Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117)
Localization
Membrane; multi-pass membrane protein.
Specificity
CFTR (CF3)
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
CFTR
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Blocking/Neutralizing 50 - 200 molar excess
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Flow usage reported in literature. ICC usage reported in literature. IHC usage was reported in scientific literature. Blocking Assay was reported in the scientific literature (PMID: 9590693). WB: Detects an approx. 170 kDa protein. Also detects one or more immunologically related proteins in murine cell line Heb7a that does not contain CFTR mRNA. IF: Staining of CFTR in mouse epithelial cells results in cell surface staining, consistent with localization at the plasma membrane. Inhibit the epithelial uptake of S. typhi in some mouse cell lines.
Theoretical MW
168 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-511 in the following applications:

Publications
Read Publications using
NB300-511 in the following applications:

  • 1 publication
  • 3 publications
  • IHC
    2 publications
  • WB
    5 publications

Reactivity Notes

Rat reactivity reported in scientific literature (see product references). Chicken reactivity reported in scientific literature (PMID: 12505864). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for CFTR Antibody (CF3)

  • ABC35
  • ABCC7CF
  • ATP-binding cassette sub-family C member 7
  • ATP-binding cassette transporter sub-family C member 7
  • cAMP-dependent chloride channel
  • CFTR
  • CFTR/MRP
  • Channel conductance-controlling ATPase
  • cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7)
  • cystic fibrosis transmembrane conductance regulator
  • cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7)
  • dJ760C5.1
  • EC 3.6.3
  • MRP7EC 3.6.3.49
  • TNR-CFTR

Background

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S.typhi intestinal submucosal uptake.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CFTR Antibody (NB300-511)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC, WB.


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FLOW
(1)
ICC/IF
(3)
IHC
(2)
WB
(5)
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Human
(6)
Mouse
(2)
Rat
(2)
Chicken
(1)
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Showing Publications 1 - 9 of 9.
Publications using NB300-511 Applications Species
Romani L, Oikonomou V, Moretti S et al. Thymosin alpha1 represents a potential potent single-molecule-based therapy for cystic fibrosis. Nat. Med. Apr 10 2017 [PMID: 28394330] (IHC, Mouse) IHC Mouse
Rottner M et al. Exaggerated apoptosis and NF-kappaB activation in pancreatic and tracheal cystic fibrosis cells. FASEB J;21(11):2939-48. 2007 Sep. [PMID: 17442732] (WB, Human) WB Human
Kulka M et al. Differential regulation of cystic fibrosis transmembrane conductance regulator by interferon gamma in mast cells and epithelial cells. J Pharmacol Exp Ther;315(2):563-70. 2005 Nov. [PMID: 16051699] (ICC/IF, Rat, Human) ICC/IF Rat, Human
Benlloch, M et al. Acceleration of glutathione efflux inhibition of gamma glutamyl transpeptidase sensitize metastic B16 melanoma cells to endothelium-induced cytotoxicity. J Biol Chem 280(8): 6950-6959. 2005 [PMID: 15561710]
Dalby-Payne JR et al. Polarization of specific tropomyosin isoforms in gastrointestinal epithelial cells and their impact on CFTR at the apical surface. Mol Biol Cell;14(11):4365-75. 2003 Nov. [PMID: 12960432] (IHC, WB, Human) IHC, WB Human
Laverty G et al. PTH stimulates a Cl(-)-dependent and EIPA-sensitive current in chick proximal tubule cells in culture. Am J Physiol Renal Physiol;284(5):F987-95. 2003 May. [PMID: 12505864] (WB, Chicken) WB Chicken
Kulka M, Gilchrist M, Duszyk M, Befus AD. Expression and functional characterization of CFTR in mast cells. J Leukoc Biol;71(1):54-64. 2002 Jan. [PMID: 11781380] (ICC/IF, FLOW, Human, Rat) ICC/IF, FLOW Human, Rat
Pier GB et al. Salmonella typhi uses CFTR to enter intestinal epithelial cells. Nature;393(6680):79-82. 1998 May 7. [PMID: 9590693] (WB, Human, Mouse) WB Human, Mouse
Walker J et al. Production and characterisation of monoclonal and polyclonal antibodies to different regions of the cystic fibrosis transmembrane conductance regulator (CFTR): detection of immunologically related proteins. J Cell Sci;108 ( Pt 6)():2433-44. 1995 Jun. [PMID: 7545688] (ICC/IF, WB, Human) ICC/IF, WB Human

Review for CFTR Antibody (NB300-511) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NB300-511:
Filter by Applications
IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence CFTR NB300-511
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reviewed by:
Anonymous
IF 05/09/2014
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Summary

ApplicationImmunofluorescence
LotNK179310
CommentsAnti-IgG(1-3)-specific secondary antibodies were non-reactive; the active immunoglobulin that labels pancreatic tissue appears to be an IgM.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CFTR Antibody (NB300-511) (0)

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Blogs on CFTR.

The ABCA1 Antibody and ABC Transporter Research
The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa...  Read full blog post.

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Recent Reviews

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Anonymous
05/09/2014
Application: IF
Species:

Bioinformatics

Gene Symbol CFTR
Entrez
Uniprot