| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-129SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13172-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77681SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46892SS | |||
| Azide and BSA Free | NBP2-80829 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-102SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-520SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-493SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75478SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56368SS | |||
![Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A) and aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0198817), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Immunohistochemistry-Paraffin-NB400-129-img0009.jpg "Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A) and aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0198817), licensed under a CC-BY license.")
![Western Blot: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 (5), HEPG2 human liver lysate (6).](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Western-Blot-NB400-129-img0004.jpg)
![Simple Western: LIMPII/SR-B2 Antibody [NB400-129] - CHAPS-solublized SVG-A (human astrocyte cells) and B103 (rat neuroblastoma cells) cell lyates, at the indicated concentrations, were probed with a 1:25 dilution of antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Simple-Western-NB400-129-img0008.jpg)
![Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A) and aged (C) urothelium incubated with Lysotracker Red (red) and co-stained with antibodies to LIMP2 (green structures) and phalloidin (blue), which labels the cortical actin cytoskeleton. Large, Lysotracker Red-positive endolysosomes are marked with white arrows. Underlying intermediate (IC) and basal cells (BC) show non-specific cytoplasmic Lysotracker Red staining that did not appear in umbrella cells (UC). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0198817), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Immunohistochemistry-Paraffin-NB400-129-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMPII/lpg85 (Green) in Hela cells using NB400-129. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Immunocytochemistry-Immunofluorescence-NB400-129-img0007.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129] - Substitution V135A enhances PSGL1 usage by mouse-adapted EV-A71. LIMPII/SR-B2 (SCARB2) and PSGL1 in various organs tissues assessed by means of immunohistochemistry. SCARB2 protein expression in CNS, intestine, pancreas, liver and lung. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31339457/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIMPII-SR-B2 Antibody-Immunohistochemistry-NB400-129-img0011.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129] - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Immunohistochemistry-NB400-129-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A), adult (B), & aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-129_rabbit-polyclonal-limpii-sr-b2-antibody-31020241530421.jpg)
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A), adult (B), & aged (C) urothelium incubated with Lysotracker Red (red) & co-stained with antibodies to LIMP2 (green structures) & phalloidin (blue), which labels the cortical actin cytoskeleton. Large, Lysotracker Red-positive endolysosomes are marked with white arrows. Underlying intermediate (IC) & basal cells (BC) show non-specific cytoplasmic Lysotracker Red staining that did not appear in umbrella cells (UC). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-129_rabbit-polyclonal-limpii-sr-b2-antibody-310202415334943.jpg)
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A), adult (B), & aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-129_rabbit-polyclonal-limpii-sr-b2-antibody-31020241534378.jpg)
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A), adult (B), & aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-129_rabbit-polyclonal-limpii-sr-b2-antibody-310202415304272.jpg)
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A), adult (B), & aged (C) urothelium incubated with Lysotracker Red (red) & co-stained with antibodies to LIMP2 (green structures) & phalloidin (blue), which labels the cortical actin cytoskeleton. Large, Lysotracker Red-positive endolysosomes are marked with white arrows. Underlying intermediate (IC) & basal cells (BC) show non-specific cytoplasmic Lysotracker Red staining that did not appear in umbrella cells (UC). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-129_rabbit-polyclonal-limpii-sr-b2-antibody-310202415291970.jpg)
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A), adult (B), & aged (C) urothelium incubated with Lysotracker Red (red) & co-stained with antibodies to LIMP2 (green structures) & phalloidin (blue), which labels the cortical actin cytoskeleton. Large, Lysotracker Red-positive endolysosomes are marked with white arrows. Underlying intermediate (IC) & basal cells (BC) show non-specific cytoplasmic Lysotracker Red staining that did not appear in umbrella cells (UC). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-129_rabbit-polyclonal-limpii-sr-b2-antibody-310202415284535.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - GADD153/CHOP Antibody [NBP2-13172] - NASTRp induces ER stress and eventually leads to cell death with Bim up-regulation. NASTRp induced ER stress and activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours and cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0122628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Western-Blot-NBP2-13172-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - GADD153/CHOP Antibody [NBP2-13172] - NASTRp induces ER stress and eventually leads to cell death with Bim up-regulation. NASTRp induced ER stress and activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours and cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0122628) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GADD153/CHOP Antibody [NBP2-13172] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GADD153/CHOP Antibody NBP2-13172 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13172-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - GADD153/CHOP Antibody [NBP2-13172] - Effects of psoralen on ER-stress related protein expression in SMMC7721. Western blot assays showing the effects of psoralen and thapsigargin on ER-stress related protein expression. Image collected and cropped by CiteAb from the following publication (//biolres.biomedcentral.com/articles/10.1186/s40659-019-0241-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Western-Blot-NBP2-13172-img0007.jpg)
![Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - Mouse liver tissue. Antigen retreival: Citrate buffer pH=6.0,Blocking: 10% BSA in 0.1%Tween in TBS, Primary antibody: 1:100 in 1% BSA in 0.1%Tween in TBS,Incubation: Overnight at 4C, Secondary antibody: 1:200 incubation for 1hr at room temperature, AB complex: 1:100 for 1hr at room temprature Develpment DAB. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-NBP2-13172-img0005.jpg)
![Flow Cytometry: GADD153/CHOP Antibody [NBP2-13172] - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody NBP2-13172F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Flow-Cytometry-NBP2-13172-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody [NBP2-13172] - CHOP/GADD153 Antibody [NBP2-13172] - WB analysis of CHOP/GADD153 in tunicamycin treated (+) and untreated (-) HeLa cell lysate.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Western-Blot-NBP2-13172-img0003.jpg)
![Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - IHC staining of CHOP/GADD153 in mouse colon using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-NBP2-13172-img0002.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - Effects of psoralen on ER-stress related protein expression in SMMC7721. a Western blot assays showing the effects of psoralen & thapsigargin on ER-stress related protein expression. b Western blot assays showing the effects of TUDC on the expression of ER-stress related protein induced by PSO. c The mRNA levels of GRP78 & DDIT3 with TUDC under PSO & TG treatment. Values are mean ± SD (n = 3) & * is means compared to the Con group, **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31277690), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-310202415291942.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - Reduction of fibronectin prevents Dex-induced ER stress in human GTM-3 cells. (A) GTM3 cells were transfected with a plasmid expressing CRISPR-Cas9 targeting fibronectin (CR-FN) & then treated with Veh or Dex for 48 hours. Fibronectin knockdown partially reduced ER stress as evident from reduced GRP78 & CHOP levels after Dex treatment (n = 2). (B) Human GTM3 cells were treated with Dex (100 nM) with or without CR-FN for 48 hours. Fixed cells were stained for fibronectin & KDEL to examine Dex-induced fibronectin & ER stress. Fibronectin knockdown reduced fibronectin & ER stress in Dex-treated TM cells (n = 2). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-310202415395954.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - TM cells are sensitive to ER stress from Dex-induced abnormal ECM compared to other ocular cells: Primary human TM cells (A), RPE (B) & corneal fibroblasts (C) were treated with Veh or Dex for 3 days & immunostained for fibronectin & KDEL. Although fibronectin was increased in all of these cell types, ER stress was only observed in TM cells. (D) Human GTM3 or ARPE-19 cells were treated with Veh or Dex for 3 days. Fibronectin & chronic ER stress markers ATF4 & CHOP were examined in cellular lysates (n = 2). Although Dex increased fibronectin in both GTM3 & ARPE-19 cells, chronic ER stress was only observed in GTM3 cells indicating that TM cells are more prone to ER stress from Dex-induced ECM accumulation. (E) GTM-3 or ARPE-19 cells were treated with cellular fibronectin (cFn) for 2 days & ER stress was examined by Western blot analysis. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-310202416121940.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - Overexpression of extracellular fibronectin induces ER stress in primary human TM cells. (A) Primary human TM cells (n = 2 strains) were treated with cellular fibronectin (10 ug/ml) for 48 hours & cellular lysates were analyzed for ER stress markers. (B) Primary human TM cells (n = 2 strains) were treated with cellular fibronectin (10 ug/ml) for 48 hours & fixed cells were stained with fibronectin & KDEL antibodies. Cellular fibronectin increased extracellular fibronectin & ER stress in GTM3 cells (n = 2). Scale bar = 50 μm. (C) GTM3 cells were transduced with fibronectin activation lentiviral particles for 48 hours & stained with fibronectin & KDEL antibodies. Increased fibronectin expression alone is sufficient to induce ER stress in TM cells (n = 2). Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-310202416113515.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - NASTRp induces ER stress & eventually leads to cell death with Bim up-regulation.(A) qRT-PCR analysis of biomarkers of ER stress/UPR related genes in NCI-H441 treated with 20 μM NASTRp in time course manner. (B) NASTRp induced ER stress & activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours & cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. (C) Upregulation of CHOP by NASTRp. NCI-H441 cells were treated with 20 NASTRp for 0–24 hours. At the indicated time points, cells were fixed & subjected to immunofluorescence assay with anti-CHOP antibody. Cell images were microphotographed using fluorescence microscopy at 60X magnification. Scale bars: white; 10 μm. (D) TUNEL-positive cells in NASTRp-treated (for 48 hours) A549 cells were counted & analyzed as relative % of TUNEL-positive cells. *P < 0.05 versus vehicle. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-31020241613624.jpg)
![Immunocytochemistry/ Immunofluorescence: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - NASTRp induces ER stress & eventually leads to cell death with Bim up-regulation.(A) qRT-PCR analysis of biomarkers of ER stress/UPR related genes in NCI-H441 treated with 20 μM NASTRp in time course manner. (B) NASTRp induced ER stress & activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours & cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. (C) Upregulation of CHOP by NASTRp. NCI-H441 cells were treated with 20 NASTRp for 0–24 hours. At the indicated time points, cells were fixed & subjected to immunofluorescence assay with anti-CHOP antibody. Cell images were microphotographed using fluorescence microscopy at 60X magnification. Scale bars: white; 10 μm. (D) TUNEL-positive cells in NASTRp-treated (for 48 hours) A549 cells were counted & analyzed as relative % of TUNEL-positive cells. *P < 0.05 versus vehicle. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-31020241612191.jpg)
![Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77681-img0006.jpg "Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77681-img0010.jpg)
![Immunohistochemistry: XBP1 Antibody [NBP1-77681] - Staining of XBP1 in mouse bladder using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-NBP1-77681-img0004.jpg)
![Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on NIH3T3 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/XBP1-Antibody-Flow-Cytometry-NBP1-77681-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77681-img0009.jpg)
![Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on HepG2 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/XBP1-Antibody-Flow-Cytometry-NBP1-77681-img0007.jpg)
![Western Blot: XBP1 Antibody [NBP1-77681] - Western Blot of PC-3 cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-77681_rabbit-polyclonal-xbp1-antibody-western-blot-672023151929..jpg)
![Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot analysis shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 2.0 ug/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (1251A) [Catalog # NBP2-46892] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Knockout-Validated-NBP2-46892-img0028.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot analysis shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 2.0 ug/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (1251A) [Catalog # NBP2-46892] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: LC3B Antibody (1251A) [NBP2-46892] - LC3B was detected in immersion fixed Chloroquine treated Hela cells (left) but was not detected in LC3B knockout Hela cells (right) using rabbit anti-human LC3B monoclonal antibody (1251A) [Catalog #NBP2-46892] at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Knockout-Validated-NBP2-46892-img0036.jpg)
![Western Blot: LC3B Antibody (1251A) [NBP2-46892] - The expression of LC3B in rat tissue. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0034.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251A) [NBP2-46892] - Jurkat cells were either untreated (A) or treated with 50uM chloroquine for 24 hours (B). An intracellular stain was performed with anti-LC3B (1251A) antibody [Catalog # NBP2-46892] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Flow-Intracellular-NBP2-46892-img0026.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Effects of PDTC on LC3 on the hippocampus of rats harvested 12 h after CLP. Values of protein data are normalized to actin level and given in relative arbitrary units. LC3-II increased in CLP rats receiving vehicle or PDTC compared with those in sham-operated rats. PDTC + CLP rats showed an increase in LC3-II compared with vehicle-treated rats. Data were expressed as mean +/- SEM, n = 6/group. *P < 0.05 vs sham-operated group. # P < 0.05 vs 12 h or Veh + CLP group. PDTC pyrrolidine dithiocarbamate, LC-3 microtubule-associated protein light chain-3, CLP cecal ligation and puncture. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26067996) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with Chloroquine for 24 hours prior to fixation, permeabilization and incubation with anti- [Catalog # NBP2-46892] and anti tubulin (NB100-690) antibodies. Image enlargement shows the accumulation of LC3 (green) on autophagosomes in response to Chloroquine treatment. Tubulin staining is shown in red and DNA is counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Immunocytochemistry-Immunofluorescence-NBP2-46892-img0032.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251A) [NBP2-46892] - IHC (Immunohistochemical) analysis of a formalin fixed and paraffin embedded tissue section of normal mouse brain using rabbit monoclonal LC3B (1251A) antibody [Catalog # NBP2-46892] at 1:100 dilution with HRP-DAB detection. The antibody generated a weak diffused cytoplasmic staining in most of the cells but some cells, especially within empty areas on the section, showed punctate signal also which signifies the presence of autophagy in those areas.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Immunohistochemistry-Paraffin-NBP2-46892-img0033.jpg)
![Flow Cytometry: LC3B Antibody (1251A) [NBP2-46892] - HeLa cells untreated or treated with 50 uM chloroquine for 24h. Image from the Alexa Fluor 405 version of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Flow-Cytometry-NBP2-46892-img0037.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Analysis shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with Chloroquine. PVDF membrane was probed with 0.2 ug/mL rabbit anti-LC3B monoclonal Antibody (1251A) (Catalog # NBP2-46892, Novus Biologicals), followed by 1:2000 dilution of goat anti-rabbit IgG secondary antibody. A specific band was detected for LC3B at a molecular weight of approximately 15 kDa in CQ treated NIH-3T3 and PC12 cell lines.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot analysis using HeLa cells treated with (+) or without (-) Chloriquine (CQ). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot image of monoclonal anti-LC3B Antibody (Clone 1251D) [Catalog # NBP2-46892]. HeLa and Neuro2A cells were treated with or without 50 uM chloroquine for 24 hours as indicated. Whole cell protein was then separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 min and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2 ug/ml anti-LC3B Antibody in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the accumulation of LC3B II upon chloroquine treatment. Bands for LC3 were detected at a molecular weight of approximately 15 kDa in treated HeLA cells, and both treated and untreated Neuro2A cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with 50 uM Chloroquine for 24 hour prior to fixation in 10% buffered formalin for 10 min. Cells were permeabilized in 0.1% Triton X-100 and incubated with 20 ug/ml anti- [Catalog # NBP2-46892] and 1:500 anti-tubulin [Catalog # NB100-690] for 1 h at room temperature. LC3B reactivity (green) was detected with ant-rabbit Dylight 488 and tubulin (red) with anti-mouse Dylight 550. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Immunocytochemistry-Immunofluorescence-NBP2-46892-img0030.jpg)
![Flow (Intracellular): LC3B Antibody (1251A) [NBP2-46892] - HeLa human cervical epithelial carcimoa cell line either treated with 50 uM chloroquine for 24 hours (filled histogram) or untreated (open histogram) was stained with Rabbit anti-Human LC3B Alexa Fluor 647 conjugated monoclonal antiobdy (1251A) [Catalog # NBP2-46892AF647]. To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer Kit (FC012-NOV).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Flow-Intracellular-NBP2-46892-img0025.jpg)
![Immunoprecipitation: LC3B Antibody (1251A) [NBP2-46892] - Western blot analysis of LC3 immunoprecipitation. 10 ug of monoclonal anti-LC3B antibody (clone 1251A) [Catalog # NBP2-46892] was used to immunoprecipitate LC3 from 200 ug of total protein from HeLa cells treated with or without 50 uM chloroquine for 24 h. Antibody-protein was captured by magnetic protein A/G beads, washed and subjected to SDS-PAGE on a 4-15% gel. Protein was transferred to 0.2 um PVDF membrane and probed with 2 ug/ml anti-LC3B (NB100-2220) and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence. The detection of LC3 at a molecular weight of approximately 12 kDa and the antibody heavy chain (Ab Hc) at a molecular weight of approximately 50 kDa is indicated.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Immunoprecipitation-NBP2-46892-img0031.jpg)
![Western Blot: LC3B Antibody (1251A) - BSA Free [NBP2-46892] - Effects of PDTC on LC3 (a), Beclin1 (b), LAMP-1 (c), & Rab7 (d) on the hippocampus of rats harvested 12 h after CLP. Values of protein data are normalized to actin level & given in relative arbitrary units. LC3-II increased while Beclin1, Rab7, & LAMP-1 declined in CLP rats receiving vehicle or PDTC compared with those in sham-operated rats. PDTC + CLP rats showed an increase in LC3-II, Beclin1, Rab7, & LAMP-1 compared with vehicle-treated rats. Data were expressed as mean ± SEM, n = 6/group. *P < 0.05 vs sham-operated group. #P < 0.05 vs 12 h or Veh + CLP group. PDTC pyrrolidine dithiocarbamate, LC-3 microtubule-associated protein light chain-3, CLP cecal ligation & puncture Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-015-0336-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-46892_rabbit-monoclonal-lc3b-antibody-1251a-310202415291956.jpg)
![Western Blot: LC3B Antibody (1251A) - BSA Free [NBP2-46892] - a LC3 in brains harvested 3, 6, 12, 24, & 48 h after CLP. b Immunofluorescence for LC3 in neurons after CLP injury. Values of protein data were related to signals obtained from actin protein & given as relative arbitrary units. LC3-II significantly increased in CLP rats at 6, 12, 24, & 48 h after surgery compared with sham-operated rats. Three-color staining for anti-LC3 antibody (green), NeuN (red), & DAPI (blue) showed that a staining pattern changes from largely diffuse to predominantly punctate & cytoplasmic in hippocampal neurons after CLP. Data were expressed as mean ± SEM, n = 6/group. *P < 0.05 vs sham-operated group. LC-3 microtubule-associated protein light chain-3, CLP cecal ligation & puncture, DAPI 4,6-diamidino-2-phenylindole Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-015-0336-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-46892_rabbit-monoclonal-lc3b-antibody-1251a-31020241612245.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. LAMP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunocytochemistry-Immunofluorescence-NB300-591-img0012.jpg "Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. LAMP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.")
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Normal deparaffinized Human placenta tissue tissues stained with anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunohistochemistry-Paraffin-NB300-591-img0015.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues stained with anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunohistochemistry-Paraffin-NB300-591-img0016.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 25ug of NRK cell lysates using anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Western-Blot-NB300-591-img0017.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Validation of subcellular fractionation and immunocytochemistry. RLC proteins are present at ER microsomes. Lysates from 7 DIV cortical neurons were subjected to differential centrifugation to isolate nuclear pellet (P1), postnuclear supernatant (PNS), mitochondrial pellet (P2), and ER microsomal pellet (P3), as depicted in the graphic on the right. Image collected and cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/embr.201744853) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Western-Blot-NB300-591-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a LAMP-2/CD107b polyclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunocytochemistry-Immunofluorescence-NB300-591-img0019.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Analysis of 10 ug of HeLa (left panel), NIH/3T3 (middle panel) and NRK (right panel) whole cell lysates using anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Western-Blot-NB300-591-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody [NB300-591] - Analysis of LAMP-2/CD107b (green) in NIH/3T3 cells.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunocytochemistry-Immunofluorescence-NB300-591-img0013.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody [NB300-591] - Both normal and cancer biopsies of deparaffinized Human tonsils were stained with anti-LAMP-2/CD107b.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-Immunohistochemistry-Paraffin-NB300-591-img0014.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - Exposure of rat primary microglial cells (rPMs) to combined antiretroviral therapy (cART) cocktail resulted in impaired lysosomal function. rPMs were seeded into six-well plates & treated with cART (5 µM each of tenofovir disoproxil fumarate (TDF), emtricitabine (FTC), & dolutegravir (DTG)) for the indicated time periods. (A,B) Exposure of microglia to cART resulted in a significant decrease in expression of lysosomal-associated membrane protein 2 (LAMP2) at 6 to 24 h post-treatment. (C) Representative bar graph showing cART-mediated significantly increased lysosomal membrane permeabilization (LMP) (24 h). (D,E) Microglia exposed to cART demonstrated a significant decrease in levels of mature cathepsin D (mCTSD) at 24 h post-treatment. (F) Representative bar graph showing exposure of cART significantly reduced the CTSD activity in rPMs (24 h). (G) Representative bar graph showing cART-mediated increased lysosomal pH in rPMs. (H,I) Acridine orange staining showing increased green color & reduced red color in cART-treated rPMs. H2O2 was used as a positive control for lysosome damage. Data is from three independent experiments. Actin beta (ACTB) served as a protein loading control for western blots. Data are expressed as means ± SEM & were analyzed using student t-test or one-way ANOVA. *, p < 0.05 vs. control; N.S., non-significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31569373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-591_rabbit-polyclonal-lamp-2-cd107b-antibody-310202416175213.jpg)
![Western Blot: LAMP-2/CD107b Antibody [NB300-591] - HSPA overexpression abrogated cART-mediated impairment of lysosomal function. Control rPMs & heat shock protein family A (HSPA) overexpressing rPMs were seeded into six-well plates subjected to various treatments for 24 h. Protein homogenates were prepared for the detection of the indicated molecules. (A,B) Representative western blots showing overexpressing HSPA in rPMs reversed cART-mediated downregulation of LAMP2 & mCTSD expression levels. (C) Representative bar graph showing overexpression of HSPA in rPMs protected lysosomal pH. (D,E) Representative bar graphs showing HSPA protected LMP (D), & CTSD activity (E) in cART-treated rPMs. For all western blots, ACTB served as a protein loading control. Data is from three independent experiments & is expressed as means ± SEM & were analyzed using student t-test or one-way ANOVA. *, p < 0.05 vs. control; #, p < 0.05 vs. cART. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31569373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-591_rabbit-polyclonal-lamp-2-cd107b-antibody-31020241618933.jpg)
![Western Blot: SR-BII Antibody [NB400-102] - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate](http://images.novusbio.com/fullsize/SR-BII-Antibody-Western-Blot-NB400-102-img0002.jpg "Western Blot: SR-BII Antibody [NB400-102] - Western blot analysis of SR-BII in 1. Human heart lysate 2. Mouse heart lysate")
![Immunocytochemistry/Immunofluorescence: SR-BII Antibody [NB400-102] - SR-BII antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/SR-BII-Antibody-Immunocytochemistry-Immunofluorescence-NB400-102-img0003.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NB300-520] - GRP78 on indicated cell extracts using NB300-520.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB300-520-img0004.jpg "Western Blot: GRP78/HSPA5 Antibody [NB300-520] - GRP78 on indicated cell extracts using NB300-520.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NB300-520] - GRP78/BIP antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NB300-520-img0005.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NB300-520] - Lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NB300-520-img0006.jpg)
![Western Blot: PINK1 Antibody [NB100-493] - Detection of murine PINK1 using NB100-493. Lane 1: molecular weight marker. Lane 2. MES cell Mitochondria (20 ug) with a band at the observed molecular weight of 63 kDa. Lane 3. MES cytosol (20 ug). Lane 4. MES nuclear (20 ug) as negative control.Lane 5. Purified human cytochrome C (0.1 ug) as PINK1 negative control.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-493-img0003.jpg "Western Blot: PINK1 Antibody [NB100-493] - Detection of murine PINK1 using NB100-493. Lane 1: molecular weight marker. Lane 2. MES cell Mitochondria (20 ug) with a band at the observed molecular weight of 63 kDa. Lane 3. MES cytosol (20 ug). Lane 4. MES nuclear (20 ug) as negative control.Lane 5. Purified human cytochrome C (0.1 ug) as PINK1 negative control.")
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0019.jpg "Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Immunocytochemistry-Immunofluorescence-H00002629-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Immunohistochemistry-Paraffin-H00002629-M01-img0014.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - GBA monoclonal antibody (M01), clone 2E2 Analysis of GBA expression in MCF-7.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0016.jpg)
![Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody (M01), clone 2E2.Lane 1: GBA transfected lysate(60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-Western-Blot-H00002629-M01-img0017.jpg)
![ELISA: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Detection limit for recombinant GST tagged GBA is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glucosylceramidase-GBA-Antibody-2E2-ELISA-H00002629-M01-img0018.jpg)
![Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.")
![<b>Biological Strategies Validation. </b>Simple Western: ATF6 Antibody [NBP1-75478] - Simple Western lane view shows a specific band for ATF6 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/ATF6-Antibody-Simple-Western-NBP1-75478-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-ATF6 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody - BSA Free [NBP1-75478] - AICAR inhibits ER stress responses. Western analysis of cell lysates of nuclear extracts. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27562249/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - Antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0002.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Noxa Antibody [NB100-56368] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using Noxa antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunohistochemistry-Paraffin-NB100-56368-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Noxa Antibody [NB100-56368] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Noxa (NB100-56368) antibody at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56368-img0003.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Western blot analysis of Noxa in A) human, B) mouse, and C) rat thymus tissue using Noxa antibody NB100-56368 at 2 ug/mL.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0001.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0007.jpg "Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-32781] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32781-img0009.jpg)
![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-32781] - Paraffin-embedded mouse duodenum. Aconitase 2 antibody [C1C3] dilution: 1:500.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32781-img0006.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE, antibody diluted at 1:20000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0004.jpg)
![Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE, antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-32781-img0005.jpg)
![Immunoprecipitation: Aconitase 2 Antibody [NBP1-32781] - HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [C1C3]. Western blot analysis was performed using Aconitase 2 antibody [C1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunoprecipitation-NBP1-32781-img0011.jpg)
