| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56437SS | |||
| BSA Free | NB100-56437 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64021-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77067SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13550-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18816SS | |||
| Azide and BSA Free | NBP3-18817 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27405 | |||
| BSA Free | NBP2-27404SS | |||
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CXCR4 Antibody - Azide Free [NBP2-24862] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-24862-img0002.jpg)
![Immunohistochemistry: CXCR4 Antibody - Azide Free [NBP2-24862] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue). Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunohistochemistry-NBP2-24862-img0003.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Analysis of 15 ug of whole cell lysate from HeLa cells with anti-CXCR4 at 1 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0001.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP1-06520-img0003.jpg "Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: Dicer Antibody [NBP1-06520] - Detection of human Dicer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Dicer antibody NBP1-06520 (lot NBP1-06520-5) used for IP at 6 ug per reaction. Dicer was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06520-4). For blotting immunoprecipitated Dicer, NBP1-06520 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunoprecipitation-NBP1-06520-img0004.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Nef does not affect siRNA loading into RISC, but inhibits its slicing activity.(A) Lysates from the U937 stable cell lines were prepared in Buffer D as described in Materials & Methods. From this, 25 µg of lysate was incubated with 32P-labeled duplex siRNA (without or with 50-fold molar excess of unlabeled siRNA) in binding buffer for 30 min at 4°C. The complexes were separated on a 6% non-denaturing polyacrylamide gel. (B) The gel shift assay was set up as in (A) except that lysates immunodepleted for either Ago2 or Dicer were also included. The positions of the mobility shifted complexes & free probe are indicated. (C) Slicer activity was assayed using immunoprecipitated Ago2 from U937/Nef-EYFP or U937/EYFP cell lysates & a 32P-labeled in vitro transcribed let7a RNA, as described in Materials & Methods. The positions of full-length RNA & the two sliced products are shown. The 59 nt marker oligonucleotide was run on the same gel, but a lower exposure is shown. (D) Densitometric analysis of autoradiograms from three independent experiments was carried out using Image J (version 1.4.1). The slicing activity in Nef-EYFP lysates is represented as a percentage of that in EYFP lysates. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06520_rabbit-polyclonal-dicer-antibody-310202416175285.jpg)
![Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - INPPL1 monoclonal antibody (M01), clone 3E6 Analysis of INPPL1 expression in HeLa.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-3E6-Western-Blot-H00003636-M01-img0004.jpg "Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - INPPL1 monoclonal antibody (M01), clone 3E6 Analysis of INPPL1 expression in HeLa.")
![ELISA: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - Detection limit for recombinant GST tagged INPPL1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-3E6-ELISA-H00003636-M01-img0005.jpg)
![Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Western-Blot-H00022943-M11-img0012.jpg "Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).")
![Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunohistochemistry-Paraffin-H00022943-M11-img0009.jpg)
![Immunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DKK1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunoprecipitation-H00022943-M11-img0010.jpg)
![ELISA: Dkk-1 Antibody (2A5) [H00022943-M11] - Detection limit for recombinant GST tagged DKK1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-ELISA-H00022943-M11-img0013.jpg)
![Immunohistochemistry: CD8 Antibody (12.C7) [NB100-64021] - scAAV8G9-optHLA-G Combo Prevents Cornea Burn-induced Vascularization and Cytotoxic T-cell Infiltration. Rabbit cornea sections were acquired 60 days following the injection of indicated vectors into burn corneas and stained for an endothelial cell marker (CD31), T cell markers, transgene abundance, and a-SMA in the indicated treatment groups. Scale bars=5um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-18002-9), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-12-C7-Immunohistochemistry-NB100-64021-img0002.jpg "Immunohistochemistry: CD8 Antibody (12.C7) [NB100-64021] - scAAV8G9-optHLA-G Combo Prevents Cornea Burn-induced Vascularization and Cytotoxic T-cell Infiltration. Rabbit cornea sections were acquired 60 days following the injection of indicated vectors into burn corneas and stained for an endothelial cell marker (CD31), T cell markers, transgene abundance, and a-SMA in the indicated treatment groups. Scale bars=5um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-18002-9), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CD8 Antibody (12.C7) [NB100-64021] - Human bile duct cancer tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-12-C7-Immunohistochemistry-Paraffin-NB100-64021-img0003.jpg)
![Flow Cytometry: CD8 alpha Antibody (12.C7) [NB100-64021] - Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 Alpha followed by Goat anti Mouse IgG (H/L):FITC](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-12-C7-Flow-Cytometry-NB100-64021-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0013.jpg "Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP")
![Immunohistochemistry: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420324853.gif)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0010.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0007.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0009.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0012.jpg)
![Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202419313214.gif)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420414588.jpg)
![Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DARC-Antibody-Western-Blot-NB100-2421-img0007.jpg "Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0009.jpg)
![Flow Cytometry: DARC Antibody [NB100-2421] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DARC-Antibody-Flow-Cytometry-NB100-2421-img0010.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - DARC (3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0008.jpg)
![Western Blot: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Fas/TNFRSF6/CD95 Antibody [NB120-13550] - Lane 1: Rat Brain Tissue Extract. Lane 2: Mouse Brain Tissue Extract.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-Western-Blot-NB120-13550-img0007.jpg "Western Blot: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Fas/TNFRSF6/CD95 Antibody [NB120-13550] - Lane 1: Rat Brain Tissue Extract. Lane 2: Mouse Brain Tissue Extract.")
![Immunocytochemistry/Immunofluorescence: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Representative IF of cerebellar sections at P10 using an anti-CD95/Fas antibody (green), an anti-calbindin antibody (red) to highlight Purkinje cells. For IF, Hoechst dye (blue) was used to mark nuclei. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-017-0838-1), licensed under a CC-BY license](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13550-img0008.jpg)
![Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Analysis of human colon tumor tissue stained with Fas polyclonal antibody, at 10ug/ml.](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunohistochemistry-Paraffin-NB120-13550-img0010.jpg)
![Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NB120-13550] - Analysis of human spleen tissue stained with Fas polyclonal antibody, at 10ug/ml.](http://images.novusbio.com/fullsize/Fas-Receptor-TNFRSF6-CD95-Antibody-Immunohistochemistry-Paraffin-NB120-13550-img0009.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Human AC16 under normoxic and hypoxic conditions. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49954-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Human AC16 under normoxic and hypoxic conditions. WB image submitted by a verified customer review.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49954] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Sequestosome-1 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunohistochemistry-NBP1-49954-img0005.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Detection of Human and Mouse Sequestosome-1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize downstream epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49954-img0004.jpg)
![Simple Western: p62/SQSTM1 Antibody [NBP1-49954] - Simple Western lane view shows a specific band for p62/SQSTM1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Simple-Western-NBP1-49954-img0003.jpg)
![Western Blot: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Western blot shows cell lysates K562, THP-1, C2C12, and C6. Membrane was probed with 1 ug/mL of (Catalog # NBP3-18816) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Western-Blot-NBP3-18816-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed U937 human myeloid leukaemia cell line but is not detected in Daudi cell line using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # NBP3-18816) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunocytochemistry-Immunofluorescence-NBP3-18816-img0002.jpg)
![Immunohistochemistry: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed paraffin-embedded sections of mouse lung tissue using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pneumocytes and alveolar cells. Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunohistochemistry-NBP3-18816-img0001.jpg)
![Simple Western: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Simple Western Lane view shows K562 and THP-1 whole cell lysate (WCL). A specific band was detected for hSTING/TMEM173 antibody (NBP3-18816) at approximately 44 kDa (as indicated) using 10 ug/mL of hSTING/TMEM173 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Simple-Western-NBP3-18816-img0004.jpg)
![Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Western-Blot-NBP2-27404-img0001.jpg "Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0009.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells and splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0006.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 0.1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0007.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0008.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on THP-1 cells with TLR3 Antibody [27N3D4] NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-177202495942.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on Raw264.7 cells with TLR3 [27N3D4] Antibody NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-17720241044614.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0015.jpg "Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Immunohistochemistry-Paraffin-NB100-594-img0016.jpg)
![Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBEF antibody used for IP at 6 ug per reaction. PBEF was also immunoprecipitated by a previous lot of this antibody and rabbit anti-Visfatin antibody. For blotting immunoprecipitated PBEF, Antibody was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PBEF-Visfatin-NAMPT-Antibody-Western-Blot-NB100-594-img0014.jpg)
