| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77477SS | |||
| Azide and BSA Free | NBP2-80624 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56167SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71005 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77257-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76963-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92995SS | |||
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0003.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).")
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0002.jpg)
![Western Blot: Survivin Antibody [NB100-56167] - Analysis of Survivin using [NB100-56167]. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombinant human survivin protein was used as a positive control. Bands observed slightly lower than the theoretical molecular weight, 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB100-56167-img0005.jpg "Western Blot: Survivin Antibody [NB100-56167] - Analysis of Survivin using [NB100-56167]. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombinant human survivin protein was used as a positive control. Bands observed slightly lower than the theoretical molecular weight, 16 kDa.")
![Immunohistochemistry: Survivin Antibody [NB100-56167] - Immunohistochemical analysis of Survivin in formalin-fixed paraffin-embedded human prostate and breast cancer using Survivin Antibody [NB100-56167]. A and A1, prostate cancer at low and high magnification, respectively. B and B1, breast cancer. B1 is a high magnification of an area of metastasis. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-NB100-56167-img0002.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56167] - Analysis of Survivin in a formalin-fixed paraffin-embedded human brain tissue microarray using Survivin Antibody [NB100-56167]. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB100-56167-img0004.jpg)
![Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-DDX0380P-100-img0005.jpg "Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.")
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of swine DC in skin and offerent lymph](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0007.jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Expression of C-type lectin receptors on human skin](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-Paraffin-DDX0380P-100-img0004.jpg)
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of human in vitro generated DC's](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0006.jpg)
![In-situ Hybridization: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - In vitro staining of swine skin DCS](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-In-situ-Hybridization-DDX0380P-100-img0008.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP1-06520-img0003.jpg "Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: Dicer Antibody [NBP1-06520] - Detection of human Dicer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Dicer antibody NBP1-06520 (lot NBP1-06520-5) used for IP at 6 ug per reaction. Dicer was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06520-4). For blotting immunoprecipitated Dicer, NBP1-06520 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunoprecipitation-NBP1-06520-img0004.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Nef does not affect siRNA loading into RISC, but inhibits its slicing activity.(A) Lysates from the U937 stable cell lines were prepared in Buffer D as described in Materials & Methods. From this, 25 µg of lysate was incubated with 32P-labeled duplex siRNA (without or with 50-fold molar excess of unlabeled siRNA) in binding buffer for 30 min at 4°C. The complexes were separated on a 6% non-denaturing polyacrylamide gel. (B) The gel shift assay was set up as in (A) except that lysates immunodepleted for either Ago2 or Dicer were also included. The positions of the mobility shifted complexes & free probe are indicated. (C) Slicer activity was assayed using immunoprecipitated Ago2 from U937/Nef-EYFP or U937/EYFP cell lysates & a 32P-labeled in vitro transcribed let7a RNA, as described in Materials & Methods. The positions of full-length RNA & the two sliced products are shown. The 59 nt marker oligonucleotide was run on the same gel, but a lower exposure is shown. (D) Densitometric analysis of autoradiograms from three independent experiments was carried out using Image J (version 1.4.1). The slicing activity in Nef-EYFP lysates is represented as a percentage of that in EYFP lysates. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06520_rabbit-polyclonal-dicer-antibody-310202416175285.jpg)
![Immunocytochemistry/Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT1.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-02340-img0008.jpg "Immunocytochemistry/Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT1.")
![Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Human liver tissue using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02340-img0007.jpg)
![Flow Cytometry: IFIT1 Antibody (OTI3G8) [NBP2-02340] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFIT1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Flow-Cytometry-NBP2-02340-img0001.jpg)
![Western Blot: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT1 monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Western-Blot-NBP2-02340-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Immunofluorescent staining of HT29 cells using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-02340-img0003.jpg)
![Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02340-img0004.jpg)
![Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02340-img0005.jpg)
![Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Human Kidney tissue using anti-IFIT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-02340-img0006.jpg)
![Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Abdominal Aortic Aneurysm (AAA) Tissues (Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Immunohistochemistry-Paraffin-NBP1-77257-img0011.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Western blot analysis of IL-23 in Raji lysate with IL-23 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0003.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 30 ng of human IL-23 recombinant protein.Antibodies: IL-23 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0009.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 20ug of lysates per lane.Antibodies: IL-23, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0010.jpg)
![Immunohistochemistry: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Staining of human pancreas tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Immunohistochemistry-NBP1-77257-img0005.jpg)
![Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. a qRT-PCR analysis of indicated genes from back skin RNA of con & ko at indicated ages (n > = 3/3, two-tailed unpaired t-test). All normalized to GAPDH. b Representative toluidine blue staining for barrier defect in con & ko mice at indicated ages (P: E15.5 embryos used as positive controls; 2 days (2d) n: 6/5, 6 days(6d) n: 4/5, 3 independent experiments). c Representative immunofluorescence staining & quantification of IL-23p19 of con & ko back skin at indicated ages (n: 3/3, two-tailed paired t-test). Scale bar: 100 µm. d Representative sorting strategy of CD45+, alpha 6H, & alpha 6L cells from con & ko epidermal cells & qRT-PCR analysis of IL-23A & IL-17A expression in sorted cells (n: 3/3, mean ± SD, two-tailed unpaired t-test, 3 independent sortings). e Representative immunoblots & quantification of indicated proteins from epidermal lysates of 7–8-week old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test). f Representative immunoblots & quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7–8-week-old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-31020241616094.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-271120241923687.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunocytochemical analysis of Raji cells using anti-IL-23A/IL-23 P19 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-2711202419562127.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-2711202419392713.gif)
![Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation in Human Raji Cells. Loading: 15 ug of lysates per lane.Antibodies: IL-23A/IL-23 P19 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Absence of blocking peptideLane B: Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-2711202419381757.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0008.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in HeLa Cells. Loading: 15 ug of lysate per lane. Antibodies: Bim NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate. Bim antibody can detect all three human isoforms, including EL, L and S isoforms.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0009.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0010.jpg)
![Western Blot: Bim Antibody - BSA Free [NBP1-76963] - Figure 3 Western Blot Validation in Human TissueLoading: 15 u of lysates per lane.Antibodies: BIM , (0.5 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human thymusLane 2: Human colon](http://images.novusbio.com/fullsize/nbp1-76963_rabbit-polyclonal-bim-antibody-2711202420115554.gif)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-76963] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Bim with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76963_rabbit-polyclonal-bim-antibody-271120242045646.gif)
![Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: MCF-7 cell lysate Lane 3: PC-12 cell lysate Lane 4: Daudi cell lysate](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Western-Blot-NBP2-67324-img0009.jpg "Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of CCR7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: MCF-7 cell lysate Lane 3: PC-12 cell lysate Lane 4: Daudi cell lysate")
![Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunohistochemistry-Paraffin-NBP2-67324-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CCR7 Antibody (SR36-04) [NBP2-67324] - Staining CCR7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunocytochemistry-Immunofluorescence-NBP2-67324-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR7 Antibody (SR36-04) [NBP2-67324] - Analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR7-Antibody-SR36-04-Immunohistochemistry-Paraffin-NBP2-67324-img0003.jpg)
![Western Blot: CCR7 Antibody (SR36-04) [NBP2-67324] - Curcumae radix extract treatment exhibited anti-metastatic effects on primary tumor of MMTV-PyMT transgenic mouse. (A) Quantitative analysis of real-time PCR was done using genes associated with breast cancer metastasis. The expression levels of Ccr7, c-Fos, Mmp9, Ccl21, & Ccl19 were determined by qRT-PCR. The Hprt gene was used as input control, respectively, * p < 0.05 & ** p < 0.005 vs. control. (B) Western blot of proteins extracted from tumor tissues in the PyMT transgenic model against Ccr7 & Gapdh. Histogram data was presented as mean ± SEM. * p < 0.05 vs. control. (C) Western blot of proteins extracted from tumor tissues against Phosphorylated Akt, Phosphorylated Ampk, Her2, ERalpha, Gapdh & beta -actin. (D) Quantitative analysis of real time PCR was done by using genes associated with apoptosis. The expression levels of Jun, Akt, & Tnf were determined by qRT-PCR. Data are representative of three independent experiments & are expressed as the mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30781353), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-67324_rabbit-monoclonal-ccr7-antibody-sr36-04-3102024161224.jpg)
![Western Blot: CCR10/GPR2 Antibody [NB100-56319] - Analysis for GPR2/CCR10 using NB100-56319 at 2 ug/ml dilution against 10 ug of MCF-7 (a human breast cancer cell line) cell lysate.](http://images.novusbio.com/fullsize/CCR10-GPR2-Antibody-Western-Blot-NB100-56319-img0002.jpg "Western Blot: CCR10/GPR2 Antibody [NB100-56319] - Analysis for GPR2/CCR10 using NB100-56319 at 2 ug/ml dilution against 10 ug of MCF-7 (a human breast cancer cell line) cell lysate.")
![Simple Western: CCR10/GPR2 Antibody [NB100-56319] - Simple Western lane view shows a specific band for CCR10 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CCR10-GPR2-Antibody-Simple-Western-NB100-56319-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer toTakada et al, 2004 for further details). PTD-p65-P6 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM, where as the control peptide, PTD or p65-P6 without PTD did not have any effect.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529--p-Ser536]-Inhibitor-Peptide-Set-Western-Blot-NBP2-29321-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer toTakada et al, 2004 for further details). PTD-p65-P6 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM, where as the control peptide, PTD or p65-P6 without PTD did not have any effect.")
![<b>Biological Strategies Validation. </b>Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Effect of various p65-inhibitor peptides on binding of purified recombinant p65 protein to the DNA in vitro. Recombinant p65 protein (100 ng/sample) was incubated for 30 minutes with 50uM of various peptides in 0.025 ml and then assayed for DNA binding activity by EMSA (Please refer to Takada et al, 2004 (3) for further details).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529--p-Ser536]-Inhibitor-Peptide-Set-Western-Blot-NBP2-29321-img0013.jpg)
![Functional (Inhibition): RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29321) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-p-Ser529-p-Ser536-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-29321-img0012.jpg)
![Western Blot: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p62/SQSTM1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Knockout-Validated-NBP2-92995-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p62/SQSTM1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - Western blot shows lysates of A172 human glioblastoma cell line and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Western-Blot-NBP2-92995-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - p62/SQSTM1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with chloroquine (left) or untreated (right) using Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Immunocytochemistry-Immunofluorescence-NBP2-92995-img0002.jpg)
![Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - p62/SQSTM1 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog #NBP2-92995) at 10 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Immunohistochemistry-Paraffin-NBP2-92995-img0003.jpg)
![Simple Western: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - Simple Western lane view shows lysates of RT-4 human bladder carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for p62/SQSTM1 at approximately 64 kDa (as indicated) using 20 ug/mL of Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Simple-Western-NBP2-92995-img0005.jpg)
![Western Blot: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: THP-1 cell lysate](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Western-Blot-NBP2-67700-img0004.jpg "Western Blot: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of CCR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: THP-1 cell lysate")
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CCR2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0005.jpg)
![Flow Cytometry: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of K562 cells with CCR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Flow-Cytometry-NBP2-67700-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CCR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (SN707) [NBP2-67700] - Analysis of paraffin-embedded human tonsil tissue using anti-CCR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCR2-Antibody-SN707-Immunohistochemistry-Paraffin-NBP2-67700-img0003.jpg)