| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77067SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27224 | |||
| BSA Free | NBP2-27223SS | |||
| Azide and BSA Free | NBP2-27224SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18816SS | |||
| Azide and BSA Free | NBP3-18817 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27405 | |||
| BSA Free | NBP2-27404SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27221 | |||
| BSA Free | NBP2-27220SS | |||
| Azide and BSA Free | NBP2-27221SS | |||
| Azide and BSA Free | NBP2-27221-0.5mg | |||
![Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DARC-Antibody-Western-Blot-NB100-2421-img0007.jpg "Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0009.jpg)
![Flow Cytometry: DARC Antibody [NB100-2421] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DARC-Antibody-Flow-Cytometry-NB100-2421-img0010.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - DARC (3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0008.jpg)
![Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - INPPL1 monoclonal antibody (M01), clone 3E6 Analysis of INPPL1 expression in HeLa.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-3E6-Western-Blot-H00003636-M01-img0004.jpg "Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - INPPL1 monoclonal antibody (M01), clone 3E6 Analysis of INPPL1 expression in HeLa.")
![ELISA: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - Detection limit for recombinant GST tagged INPPL1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-3E6-ELISA-H00003636-M01-img0005.jpg)
![Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Western-Blot-H00022943-M11-img0012.jpg "Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).")
![Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunohistochemistry-Paraffin-H00022943-M11-img0009.jpg)
![Immunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DKK1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunoprecipitation-H00022943-M11-img0010.jpg)
![ELISA: Dkk-1 Antibody (2A5) [H00022943-M11] - Detection limit for recombinant GST tagged DKK1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-ELISA-H00022943-M11-img0013.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP1-06520-img0003.jpg "Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: Dicer Antibody [NBP1-06520] - Detection of human Dicer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Dicer antibody NBP1-06520 (lot NBP1-06520-5) used for IP at 6 ug per reaction. Dicer was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06520-4). For blotting immunoprecipitated Dicer, NBP1-06520 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunoprecipitation-NBP1-06520-img0004.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Nef does not affect siRNA loading into RISC, but inhibits its slicing activity.(A) Lysates from the U937 stable cell lines were prepared in Buffer D as described in Materials & Methods. From this, 25 µg of lysate was incubated with 32P-labeled duplex siRNA (without or with 50-fold molar excess of unlabeled siRNA) in binding buffer for 30 min at 4°C. The complexes were separated on a 6% non-denaturing polyacrylamide gel. (B) The gel shift assay was set up as in (A) except that lysates immunodepleted for either Ago2 or Dicer were also included. The positions of the mobility shifted complexes & free probe are indicated. (C) Slicer activity was assayed using immunoprecipitated Ago2 from U937/Nef-EYFP or U937/EYFP cell lysates & a 32P-labeled in vitro transcribed let7a RNA, as described in Materials & Methods. The positions of full-length RNA & the two sliced products are shown. The 59 nt marker oligonucleotide was run on the same gel, but a lower exposure is shown. (D) Densitometric analysis of autoradiograms from three independent experiments was carried out using Image J (version 1.4.1). The slicing activity in Nef-EYFP lysates is represented as a percentage of that in EYFP lysates. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06520_rabbit-polyclonal-dicer-antibody-310202416175285.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0013.jpg "Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP")
![Immunohistochemistry: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420324853.gif)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0010.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0007.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0009.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0012.jpg)
![Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202419313214.gif)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420414588.jpg)
![Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Western-Blot-NBP2-27223-img0004.jpg "Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained before testing by intracellular staining. Fixed and permeabilized cells were incubated with 10 uL (0.5 ug) of TNF- alpha antibody for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Flow-Cytometry-NBP2-27223-img0002.jpg)
![Inhibition Activity: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit . Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Inhibition-Activity-NBP2-27223-img0006.jpg)
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0008.jpg "Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in Raw 264.7 cell lysate.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP1-78427-img0003.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse smooth muscle using DAB wtih hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0007.jpg)
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with TLR4 Antibody NBP1-78427AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0009.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0006.jpg)
![Western Blot: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Western blot shows cell lysates K562, THP-1, C2C12, and C6. Membrane was probed with 1 ug/mL of (Catalog # NBP3-18816) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Western-Blot-NBP3-18816-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed U937 human myeloid leukaemia cell line but is not detected in Daudi cell line using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # NBP3-18816) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunocytochemistry-Immunofluorescence-NBP3-18816-img0002.jpg)
![Immunohistochemistry: STING/TMEM173 Antibody (2292D) [NBP3-18816] - STING/TMEM173 was detected in immersion fixed paraffin-embedded sections of mouse lung tissue using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pneumocytes and alveolar cells. Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2292D-Immunohistochemistry-NBP3-18816-img0001.jpg)
![Simple Western: STING/TMEM173 Antibody (2922D) [NBP3-18816] - Simple Western Lane view shows K562 and THP-1 whole cell lysate (WCL). A specific band was detected for hSTING/TMEM173 antibody (NBP3-18816) at approximately 44 kDa (as indicated) using 10 ug/mL of hSTING/TMEM173 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/STING-TMEM173-Antibody-2922D-Simple-Western-NBP3-18816-img0004.jpg)
![Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-8F4-Immunohistochemistry-Frozen-NBP1-51148-img0001.jpg "Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.")
![Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Human AC16 under normoxic and hypoxic conditions. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49954-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Human AC16 under normoxic and hypoxic conditions. WB image submitted by a verified customer review.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49954] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Sequestosome-1 used at a dilution of 1:1,000 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunohistochemistry-NBP1-49954-img0005.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: p62/SQSTM1 Antibody [NBP1-49954] - Detection of Human and Mouse Sequestosome-1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize downstream epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-49954-img0004.jpg)
![Simple Western: p62/SQSTM1 Antibody [NBP1-49954] - Simple Western lane view shows a specific band for p62/SQSTM1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Simple-Western-NBP1-49954-img0003.jpg)
![Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Western-Blot-NBP2-27404-img0001.jpg "Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0009.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells and splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0006.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 0.1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0007.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0008.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on THP-1 cells with TLR3 Antibody [27N3D4] NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-177202495942.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) - BSA Free [NBP2-27404] - An intracellular stain was performed on Raw264.7 cells with TLR3 [27N3D4] Antibody NBP2-27405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/nbp2-27404_rat-monoclonal-tlr3-antibody-27n3d4-17720241044614.jpg)
![Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cytometry-NBP2-27220-img0002.jpg "Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).")
![Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - Analysis using the PE conjugate of NBP2-27220. Staining of Con A stimulated human PBMCs with PE-conjugated human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0004.jpg)
![<b>Biological Strategies Validation. </b>Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - hPBMC cells were either stimulated with Con A (panel B) or grown in normal media (panel A). A cell surface stain was performed with TIM-3 (F38-2E2) NBP2-27220 and Mouse IgG1 Kappa NBP2-27287. Cells were incubated with antibody for 20 minutes. A co-stain was also performed with CD4 (RPA-T4) NBP2-27216AF647.](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0005.jpg)