Immunology reagents: Novus Biologicals

NBP2-24729

TLR9 Antibody (26C593.2) - BSA Free

(4 Reviews)

(160 Publications)

0.1 mg

NBP2-24729

<b>Genetic Strategies Validation. </b>Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY license.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of colonic LP or peritoneal cavity (PerC) of normal mice were evaluated by flow cytometry. B cells were intracellularly stained with the anti-TLR9 antibody after the cell surface staining with anti-B220 and CD5 antibodies, and examined using flow cytometry. N = 3, performed twice. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0146191) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: TLR9 Antibody (26C593.2) [NBP2-24729] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated genotypes. For experiments, cells were stimulated through the BCR (green) for 30 minutes then fixed and stained for TLR9 (blue) and Lamp-1 (red)(n = 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0089792) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [NBP2-24729] - Monkey retina tissue. Image from verified customer review.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Gating strategy for the detection of TLR-7 and -9 in B cell subsets. PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-9. FSC and SSC were first used to gate out debris and SSC-A and SSC-H was utilized to eliminate duplicates. Further gating was done on CD45 and CD19, to target B cells. To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-9 expression on these subsets. Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0120383) licensed under a CC-BY license.

Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 on turbinate epithelial cells from healthy controls compared to turbinate and polyp epithelial cells from patients with CRSwNP, n = 5 (A). Intracellular staining for TLR9 (open histogram, black line) and isotype control (filled histogram) on turbinate epithelial cells from a healthy control (B), turbinate epithelial cells (C) and polyp epithelial cells from a patient (D), analysed using flow cytometry. Results are presented as mean +/- SEM, **P<0.01. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0105618) licensed under a CC-BY license.

THP-1 human acute monocytic leukemia cell line was stained with Mouse anti-TLR9 (26C593.2) Protein-G purified Monoclonal Antibody conjugated to Janelia Fluor® 549 (Catalog # NBP2-24729JF549, blue histogram) or matched control antibody (orange histogram).

Alveolar epithelial cells (AECs), fibroblasts and fibrocytes all express Toll-like receptor-9. AECs, fibroblasts, fibrocytes and whole lungs were isolated from Balb/c, mice and total RNA prepared and analyzed for TLR-9 expression. The data were normalized to the expression level of TLR-9 in dendritic cells, which was set to 1 (n = 3/group). No TLR-9 message was amplified in cells from TLR-9-/- mice. (B, C) Immunohistochemistry was performed on paraffin wax-embedded whole-lung tissue from normal Balb/c mice. (B) Control staining with secondary antibody only; (C) diffuse TLR-9 staining, with notable staining in the bronchial epithelium, at the alveolar junctions and the interstitium. Fibroblasts (E) and AECs (G) were isolated from Balb/c mice and fixed. Immunofluorescence staining for TLR-9 shows that both cell types expressed TLR-9. The control slides with secondary antibody alone (D and F) did not show any auto-fluorescence or non-specific staining. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21810214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca, Eq, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Eq, Pm, Pm

Applications:

, , , , , , , , , , ,

Applications: , , , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-305A

Alt Catalog# IMG-305A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24729AF350
Alexa Fluor 405	NBP2-24729AF405
Alexa Fluor 488	NBP2-24729AF488
Alexa Fluor 532	NBP2-24729AF532
Alexa Fluor 594	NBP2-24729AF594
Alexa Fluor 647	NBP2-24729AF647
Alexa Fluor 700	NBP2-24729AF700
Alexa Fluor 750	NBP2-24729AF750
Allophycocyanin	NBP2-24729APC
Biotin	NBP2-24729B
Biotin	NBP2-24890
CoraFluor 1	NBP2-24729CL1
DyLight 350	NBP2-24729UV
DyLight 405	NBP2-24729V
DyLight 488	NBP2-24729G
DyLight 550	NBP2-24729R
DyLight 594	NBP2-24729DL594
DyLight 650	NBP2-24729C
DyLight 680	NBP2-24729FR
DyLight 755	NBP2-24729IR
FITC	NBP2-24729F
HRP	NBP2-24729H
Janelia Fluor 525	NBP2-24729JF525
Janelia Fluor 549	NBP2-24729JF549
Janelia Fluor 646	NBP2-24729JF646
Janelia Fluor 669	NBP2-24729JF669
PE	NBP2-24907
PE	NBP2-24729PE
PerCP	NBP2-24729PCP
mFluor Violet 450 SE	NBP2-24729MFV450
mFluor Violet 500 SE	NBP2-24729MFV500
mFluor Violet 610 SE	NBP2-24729MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-24863
BSA Free	NBP2-24729SS

compare

(select up to 3 products from this page to compare)

NBP2-24906

TLR7 Antibody - BSA Free

(4 Reviews)

(82 Publications)

0.1 mg

NBP2-24906

Immunocytochemistry/Immunofluorescence: TLR7 Antibody [NBP2-24906] - Immunolocalization of TLR7 (A) and glial fibrillary acidic protein (GFAP) (B) in murine myenteric plexus. Most of the GFAP positive cells were also positive for TLR7 in the merged image (C, yellow staining). (Courtesy of Barajon I, et al., Journal of Histochemistry and Cytochemistry, 57(11): 1013-;1023, 2009.)

Immunohistochemistry: TLR7 Antibody [NBP2-24906] - Expression of the TLRs 5 and 7 were evaluated in the PDAC tissue by their staining intensity using TLR5 antibody (NBP2-24787) and TLR7 antibody (NBP2-24906). Note negative staining in E and G; positive staining in F and H. For TLR7 immunopositivity was detectable in the cytoplasm with no notable membranous or nuclear positivity. TLR5 showed distinctive nuclear positivity, and detectable positivity in the cytoplasm in some samples. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0219245) licensed under a CC-BY license.

Immunohistochemistry-Frozen: TLR7 Antibody [NBP2-24906] - analysis of frozen mouse ear skin tissue using anti-TLR7 antibody. Image from verified customer review.

Immunohistochemistry-Frozen: TLR7 Antibody [NBP2-24906] - analysis of TLR7 in acetone-fixed, frozen mouse ear skin section using anti-TLR7 antibody. Image from verified customer review.

Western Blot: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Detection of TLR7 in RAW cell lysate using this antibody.

Immunocytochemistry/Immunofluorescence: TLR7 Antibody [NBP2-24906] - Analysis using the Azide Free version of NBP2-24906. Staining of Raw 246.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.

Immunohistochemistry-Paraffin: TLR7 Antibody - BSA Free [NBP2-24906] - Analysis of a FFPE tissue section of human skin using 1:200 dilution of TLR7 antibody (NBP2-24906). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Immunohistochemistry-Paraffin: TLR7 Antibody - BSA Free [NBP2-24906] - Analysis of human colon tissue using NBP2-24905 (top) and an isotype control (bottom) at 5 ug/ml.

Immunohistochemistry: TLR7 Antibody - BSA Free [NBP2-24906] - Expression of TLR7 in lung tissues of non-smokers, smokers, & COPD patients. Total protein & mRNA were obtained from lung tissues of non-smokers (n = 15), smokers (n = 12), & COPD patients (n = 15). TLR7 protein & mRNA expression were determined by western blot (A) & real time PCR (B) in lung parenchyma. (A) Representative images of western blot for TLR7 & corresponding densitometry expressed as ratio to beta -actin. (B) TLR7 mRNA expression given as the ratio to GAPDH. (C, D, E) Lung sections were immunostained for TLR7 & quantified by means of immunohistochemical score of TLR7 in alveolar macrophages (C) & bronchial epithelial cells (D). (E) Representative immunohistochemistry images are shown. The control IgG isotype showed negative staining. Data are presented as individual values & mean ± standard deviation. Exact P values were obtained using Kruskal-Wallis & Dunn’s post-hoc tests. Image collected & cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-015-0179-5), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of TLR7 in brain tissues at different time points after induction of status epilepticus.Quantitative real-time PCR analysis of the expression of TLR7 mRNA in the hippocampus (a) and cortex (b) at different time points after induction of SE (n = 3). ns not significant; *P < 0.05; ****P < 0.0001; one-way ANOVA with Tukey’s post hoc test. Bars represent the mean +/- SEM. The expression of FL-TLR7 and C-terminal TLR7 in the hippocampus (c, d) and temporal cortex (e, f) on the third day after induction of SE by intrahippocampal injection with KA were both significantly higher than that of the control group (n = 9 mice per group). **P < 0.01; unpaired two-tailed Student’s t-test. Bars represent the mean +/- SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37258573), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-581A

Alt Catalog# IMG-581A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24906AF350
Alexa Fluor 405	NBP2-24906AF405
Alexa Fluor 488	NBP2-24906AF488
Alexa Fluor 532	NBP2-24906AF532
Alexa Fluor 594	NBP2-24906AF594
Alexa Fluor 647	NBP2-24906AF647
Alexa Fluor 700	NBP2-24906AF700
Alexa Fluor 750	NBP2-24906AF750
Allophycocyanin	NBP2-24906APC
Biotin	NBP2-24906B
CoraFluor 1	NBP2-24906CL1
DyLight 350	NBP2-24906UV
DyLight 405	NBP2-24906V
DyLight 488	NBP2-24906G
DyLight 550	NBP2-24906R
DyLight 594	NBP2-24906DL594
DyLight 650	NBP2-24906C
DyLight 680	NBP2-24906FR
DyLight 755	NBP2-24906IR
FITC	NBP2-24777
HRP	NBP2-24906H
Janelia Fluor 525	NBP2-24906JF525
Janelia Fluor 549	NBP2-24906JF549
Janelia Fluor 646	NBP2-24906JF646
Janelia Fluor 669	NBP2-24906JF669
PE	NBP2-31071
PE	NBP2-24906PE
PerCP	NBP2-24906PCP
mFluor Violet 450 SE	NBP2-24906MFV450
mFluor Violet 500 SE	NBP2-24906MFV500
mFluor Violet 610 SE	NBP2-24906MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-24905
Azide and BSA Free	NBP2-24905SS
BSA Free	NBP2-24906SS

compare

(select up to 3 products from this page to compare)

NB600-235

BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

(2 Reviews)

(52 Publications)

0.1 ml

NB600-235

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.

Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.

Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-BLIMP1/PRDM1 (3H2-E8) conjugated to DyLight 550 [NB600-235R] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ).

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on U266 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 [3H2-E8] Antibody NB600-235C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - The effect of IL-21 & CD40L exposure on MEC1 & MEC2 cells.Expression of EBNA-2 & LMP-1 in IL-21 treated cells (A, B). (A) Simultaneous immunofluorescence staining of EBNA-2 (Green) & LMP-1 (Red); magnification (×100), scale bar 25 µm. Note the downregulation of EBNA-2 & upregultion of LMP-1 after IL-21 treatment. (B) Expression of EBNA-2, LMP-1 & Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5×105 cells were loaded in the control lanes & 5×105 were loaded in both untreated & IL-21 treated MEC1 & MEC2 lanes. Note low expression of EBNA-2 & high expression of LMP-1 after IL-21 treatment & induction of Blimp-1 after IL-21 treatment. (C) Activity of the W & C promoters that regulate EBNA-2 expression & LMP-1 mRNA expression by Q-PCR. Note the difference in EBNA-2 regulation; the MEC2 cell uses both Wp & Cp while in MEC1 only Wp is active. (D) Expression of EBNA-2 & LMP-1 in cells exposed to CD40L. Simultaneous immunofluorescence staining; for details see (A). Note: EBNA-2 & LMP-1 are downregulated by CD40L in both lines. (E) CD40L induced modulation of surface marker by FACS analysis. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Po

Species Glossary

Reactivity: Hu, Mu, Po

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-235AF350
Alexa Fluor 405	NB600-235AF405
Alexa Fluor 488	NB600-235AF488
Alexa Fluor 532	NB600-235AF532
Alexa Fluor 594	NB600-235AF594
Alexa Fluor 647	NB600-235AF647
Alexa Fluor 700	NB600-235AF700
Alexa Fluor 750	NB600-235AF750
Allophycocyanin	NB600-235APC
Biotin	NB600-235B
CoraFluor 1	NB600-235CL1
DyLight 350	NB600-235UV
DyLight 405	NB600-235V
DyLight 488	NB600-235G
DyLight 550	NB600-235R
DyLight 594	NB600-235DL594
DyLight 650	NB600-235C
DyLight 680	NB600-235FR
DyLight 755	NB600-235IR
FITC	NB600-235F
HRP	NB600-235H
Janelia Fluor 525	NB600-235JF525
Janelia Fluor 549	NB600-235JF549
Janelia Fluor 646	NB600-235JF646
Janelia Fluor 669	NB600-235JF669
PE	NB600-235PE
PerCP	NB600-235PCP
mFluor Violet 450 SE	NB600-235MFV450
mFluor Violet 500 SE	NB600-235MFV500
mFluor Violet 610 SE	NB600-235MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-235SS
Azide and BSA Free	NBP2-80597

compare

(select up to 3 products from this page to compare)

NB100-65805

CD81 Antibody (1D6) - BSA Free

(1 Review)

(26 Publications)

0.1 ml

NB100-65805

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody (1D6) NB100-65805 (blue) and a matched isotype control MAB002 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

Western Blot: CD81 Antibody (1D6) [NB100-65805] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Staining of human peripheral blood lymphocytes.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: CD81 Antibody (1D6) - BSA Free [NB100-65805] - Characterization & internalization of M2-Exos. (A) TEM image of M2-Exos. Scale bar: 200 nm. (B) DLS measurement of M2-Exos size. (C) Western blotting assay of exosomal markers in THP-1-M2 cellular lysate & M2-Exos preparation. (D,F) Fluorescence images of 786-O & ACHN cells treated with or without PKH67-labeled M2-Exos (green). Scale bar: 50 μm. (E,G) Three-dimensional confocal reconstruction of 786-O & ACHN cells treated with PKH67-labeled M2-Exos (green). (H) Fluorescence staining analyzing the internalization of M2-Exos by 786-O cells over 12 h. Scale bar: 10 μm. CT: control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35328425), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Principle of EV‐GLYPH assay for early‐stage NSCLC identification. A) Glycan signatures on sEVs derived from patients with benign and malignant lung diseases. B) CT and PET images of benign and malignant lung nodules. C) Working scheme of EV‐GLYPH assay. SEC‐purified sEVs from plasma are captured by anti‐MUC1 antibody immobilized on an electrode and subsequently labeled with SERS nanotags against LacdiNAc, T antigen, and CD81. D) A nanomixing fluid flow is generated on the electrode surface by the applied ac‐EHD field. E) With in situ SERS mapping, the average Raman intensities and false‐color SERS spectral images (insets) are established based on the characteristic Raman signals of three SERS nanotags (WFA‐MBA, 1075 cm−1, red; PNA‐TFMBA, 1375 cm−1, blue; anti‐CD81‐DTNB, 1335 cm−1, green), representing the expression of LacdiNAc, T antigen, and CD81, respectively. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38885350), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Gt, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Gt, Pm, Sh

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-65805AF350
Alexa Fluor 405	NB100-65805AF405
Alexa Fluor 488	NB100-65805AF488
Alexa Fluor 532	NB100-65805AF532
Alexa Fluor 594	NB100-65805AF594
Alexa Fluor 647	NB100-65805AF647
Alexa Fluor 700	NB100-65805AF700
Alexa Fluor 750	NB100-65805AF750
Allophycocyanin	NB100-65805APC
Allophycocyanin/Cy7	NB100-65805APCCY7
Biotin	NB100-65805B
CoraFluor 1	NB100-65805CL1
DyLight 350	NB100-65805UV
DyLight 405	NB100-65805V
DyLight 488	NB100-65805G
DyLight 550	NB100-65805R
DyLight 594	NB100-65805DL594
DyLight 650	NB100-65805C
DyLight 680	NB100-65805FR
DyLight 755	NB100-65805IR
FITC	NB100-65805F
FITC	NB100-64060
HRP	NB100-65805H
Janelia Fluor 525	NB100-65805JF525
Janelia Fluor 549	NB100-65805JF549
Janelia Fluor 646	NB100-65805JF646
Janelia Fluor 669	NB100-65805JF669
PE	NB100-65805PE
PE/Atto594	NB100-65805PEATT594
PE/Cy5.5	NB100-65805PECY55
PE/Cy7	NB100-65805PECY7
PerCP	NB100-65805PCP
mFluor Violet 450 SE	NB100-65805MFV450
mFluor Violet 500 SE	NB100-65805MFV500
mFluor Violet 610 SE	NB100-65805MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-65805SS
Azide and BSA Free	NBP2-80660

compare

(select up to 3 products from this page to compare)

NBP2-27332

TLR7 Antibody (4G6) - BSA Free

(20 Publications)

0.1 mg

NBP2-27332

Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. THP-1 cells were stained with TLR7 (4G) NBP2-25274 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.

Western Blot: TLR7 Antibody (4G6) - BSA Free [NBP2-27332] - The protein of TLR7 were pull down by miR-138-biotin / miR-mut-138-biotin with Streptavidin agarose beads in BV2 cells. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC7710131/) licensed under a CC-BY license.

Immunohistochemistry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of TLR7 [4G6] antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Ramos cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - TLR7 antibodies tested at 2 ug/ml on recombinant partial hTLR7 protein (amino acids 562-839). NB100-56682 and NBP2-24767 immunogen sequences are not present in recombinant protein; *NB100-56588 is not recommended for use in western blot.

Western Blot: TLR7 Antibody (4G6) [NBP2-27332] - Analysis using Azide Free version of NBP2-27332. Human TLR7 antibody in Ramos and transfected 293T lysate using TLR7 monoclonal antibody at 10 ug/ml.

Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - Analysis of TLR7 in human monocytes using 2 ug of TLR7 monoclonal antibody (red) and 2 ug of mouse IgG1 isotype control antibody (green). TLR intracellular flow kit was used for this test, and an anti-mouse IgG PE conjugated secondary antibody .

Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Daudi cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Flow Cytometry: TLR7 Antibody (4G6) [NBP2-27332] - An intracellular stain was performed on Raji cells with TLR7 Antibody (4G6) NBP2-27332 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Western Blot: TLR7 Antibody (4G6) - BSA Free [NBP2-27332] - ADEV miR‐138 interacts with murine TLR7 in the endosomes. (A) Representative fluorescence images of mouse primary microglial cells incubated with Exo‐Fect+ADEVs+Cy5‐miR138 or Exo‐Fect+Cy5‐miR138 (without ADEVs) or Exo‐Fect+ADEVs+miR138 (unstained) for 30 min followed by immunostaining of (A) an early endosome marker (EEA1, Green) & (B) TLR7 (Green). Exo‐fect+ADEVs+Cy5‐miR138, Cy5‐miR138 were loaded into ADEVs using by Exo‐Fect transfection kit; Exo‐fect+Cy5‐miR138, Cy5‐miR138 were loaded using by Exo‐Fect transfection kit (without ADEVs); Exo‐fect+ADEVs+miR138, unstained miR138 were loaded into ADEVs using Exo‐Fect transfection kit; Bars, 50 μm (n = 3). (C) TLR7 was immunoprecipitated from BV2 cells by IgG / TLR7 / LAMP1 antibody, followed by assessment of miR138 / U6 expression by real‐time PCR. One‐way ANOVA followed by Bonferroni's post hoc test was used to determine the statistical significance among multiple groups (n = 3). (D) TLR7 was immunoprecipitated from HEK‐Null / HEK‐TLR7 cells by IgG/TLR7 antibody, followed by assessment of miR138 expression by real‐time PCR. One‐way ANOVA followed by Bonferroni's post hoc test was used to determine the statistical significance among multiple groups (n = 3). (E) The protein of TLR7 were pull down by miR‐138‐biotin / miR‐mut‐138‐biotin with Streptavidin agarose beads in BV2 cells. All data are presented as mean ± SD or SEM of three individual experiments. *,P < 0.05; **,P < 0.01; ***,P < 0.001 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33304479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

miR-298-5p and miR-100-5p enter microglia, co-localize to their endosomes, and bind directly to human TLR8. a C57BL/6 microglia were incubated with 40 μg/ml pHrodo Red Dextran serving as endosomal marker for 20 min. Subsequently, microglia were exposed to 5 μg/ml of Alexa488-labeled miR-298-5p, Alexa488-labeled miR-100-5p, or Alexa488-labeled let-7b-5p for 4 h. Cells were then fixed and stained with DAPI. b In parallel, microglia exposed to Alexa488-labeled miR-298-5p or Alexa488-labeled miR-100-5p, as described above, were fixed and immunolabeled with TLR7 antibody. (a, b) Cells were analyzed by confocal microscopy with sequential analysis. Representative images of microglia incubated with the indicated fluorescent miRNAs (488 nm, green), stained with DAPI (405 nm, blue), and pHrodo Red Dextran or TLR7 (552 nm, red) are depicted. Green lines indicate region of interest (ROI); scale bar, 10 μm. Representative 3D-vertical slice images of microglia show co-localization of miRNAs and endosomes through different cellular levels (left panel). Diagrams depict fluorescence intensities of the marked ROI in microglia for the sequential analysis used (DAPI: blue line; pHrodo Red Dextran/TLR7: red line; fluorescent miRNA: green line, right panel). (c) Binding affinity measurements of purified polyhistidine-tagged human TLR8 protein and synthetic miRNAs, as indicated, using microscale thermophoresis (MST). TLR8-RNA interaction was monitored by titrating indicated miRNAs from 500 μM to 30 nM against 50 nM RED-tris-NTA-labeled hTLR8 protein and measured with the NanoTemper Monolith NT.115 MST device. Kd values were calculated from dose response curves, which were generated from titration experiments. Data are expressed as mean +/- SD, n = 4 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34838071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6675A

Alt Catalog# IMG-6675A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25274AF350
Alexa Fluor 405	NBP2-25274AF405
Alexa Fluor 488	NBP2-25274AF488
Alexa Fluor 532	NBP2-25274AF532
Alexa Fluor 594	NBP2-25274AF594
Alexa Fluor 647	NBP2-25274AF647
Alexa Fluor 700	NBP2-25274AF700
Alexa Fluor 750	NBP2-25274AF750
Allophycocyanin	NBP2-25274APC
Allophycocyanin/Cy7	NBP2-27332APCCY7
Biotin	NBP2-25274B
DyLight 350	NBP2-25274UV
DyLight 405	NBP2-25274V
DyLight 488	NBP2-25274G
DyLight 550	NBP2-25274R
DyLight 594	NBP2-27332DL594
DyLight 650	NBP2-25274C
DyLight 680	NBP2-25274FR
DyLight 755	NBP2-25274IR
FITC	NBP2-25274F
HRP	NBP2-25274H
Janelia Fluor 549	NBP2-27332JF549
Janelia Fluor 646	NBP2-27332JF646
PE	NBP2-27251
PE/Atto594	NBP2-27332PEATT594
PE/Cy5.5	NBP2-27332PECY55
PE/Cy7	NBP2-27332PECY7
PerCP	NBP2-25274PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-25274
BSA Free	NBP2-27332SS
Azide and BSA Free	NBP2-25274SS
Azide and BSA Free	NBP2-80982

compare

(select up to 3 products from this page to compare)

NBP2-25196

CD19 Antibody (CB19) - BSA Free

(1 Review)

(12 Publications)

0.1 mg

NBP2-25196

Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) [NBP2-25196] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the FITC conjugate of NBP2-25196. Staining of CD19 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD19 antibody.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the PE conjugate of NBP2-25196. Staining of CD19 in 1x10^6 human PBMC using 10 ul (0.25 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25196. Staining of human PBMC. Image from verified customer review.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - Analysis using the DyLight 405 conjugate of NBP2-25196. Staining of CD19 in human PBMCs using anti-CD19 antibody. Image from verified customer review.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with TACI/TNFRSF13B/CVID (1A1) antibody NB600-1189PE and a matched isotype control. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.

Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6321A

Alt Catalog# IMG-6321A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25196AF350
Alexa Fluor 405	NBP2-25196AF405
Alexa Fluor 488	NBP2-25196AF488
Alexa Fluor 532	NBP2-25196AF532
Alexa Fluor 594	NBP2-25196AF594
Alexa Fluor 647	NBP2-25196AF647
Alexa Fluor 700	NBP2-25196AF700
Alexa Fluor 750	NBP2-25196AF750
Allophycocyanin	NBP2-25196APC
Allophycocyanin/Cy7	NBP2-25196APCCY7
Biotin	NBP2-25196B
CoraFluor 1	NBP2-25196CL1
DyLight 350	NBP2-25196UV
DyLight 405	NBP2-25196V
DyLight 488	NBP2-25196G
DyLight 550	NBP2-25196R
DyLight 594	NBP2-25196DL594
DyLight 650	NBP2-25196C
DyLight 680	NBP2-25196FR
DyLight 755	NBP2-25196IR
FITC	NBP2-25196F
HRP	NBP2-25196H
Janelia Fluor 525	NBP2-25196JF525
Janelia Fluor 549	NBP2-25196JF549
Janelia Fluor 646	NBP2-25196JF646
Janelia Fluor 669	NBP2-25196JF669
PE	NBP2-26646
PE/Atto594	NBP2-25196PEATT594
PE/Cy5.5	NBP2-25196PECY55
PE/Cy7	NBP2-25196PECY7
PerCP	NBP2-25196PCP
mFluor Violet 450 SE	NBP2-25196MFV450
mFluor Violet 500 SE	NBP2-25196MFV500
mFluor Violet 610 SE	NBP2-25196MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-25196SS
Azide and BSA Free	NBP2-80634

compare

(select up to 3 products from this page to compare)

NBP1-44861

CD81 Antibody (M38) - BSA Free

(11 Publications)

0.1 mg

NBP1-44861

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Surface staining of CD81 in human peripheral blood with anti-CD81 (M38) APC-CyTM7.

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) APC.

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) FITC.

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Flow Cytometry: CD81 Antibody (M38) [PE] [NBP1-44861PE] - A cell surface stain was performed on THP-1 cells with CD81 antibody (M38) NBP1-44861 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE. Image using the PE form of this antibody.

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) PE.

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) biotin, streptavidin-APC.

Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 ug/ml) GAM APC.

Reactivity:

Hu, Mu, Fe, Rb

Species Glossary

Reactivity: Hu, Mu, Fe, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-44861AF350
Alexa Fluor 405	NBP1-44861AF405
Alexa Fluor 488	NBP1-44861AF488
Alexa Fluor 532	NBP1-44861AF532
Alexa Fluor 594	NBP1-44861AF594
Alexa Fluor 647	NBP1-44861AF647
Alexa Fluor 700	NBP1-44861AF700
Alexa Fluor 750	NBP1-44861AF750
Allophycocyanin	NBP1-44861APC
Allophycocyanin/Cy7	NBP1-44861APCCY7
Biotin	NBP1-44861B
CoraFluor 1	NBP1-44861CL1
DyLight 350	NBP1-44861UV
DyLight 405	NBP1-44861V
DyLight 488	NBP1-44861G
DyLight 550	NBP1-44861R
DyLight 594	NBP1-44861DL594
DyLight 650	NBP1-44861C
DyLight 680	NBP1-44861FR
DyLight 755	NBP1-44861IR
FITC	NBP1-44859
HRP	NBP1-44861H
Janelia Fluor 525	NBP1-44861JF525
Janelia Fluor 549	NBP1-44861JF549
Janelia Fluor 646	NBP1-44861JF646
Janelia Fluor 669	NBP1-44861JF669
PE	NBP1-44861PE
PE/Atto594	NBP1-44861PEATT594
PE/Cy5.5	NBP1-44861PECY55
PE/Cy7	NBP1-44861PECY7
PerCP	NBP1-44861PCP
mFluor Violet 450 SE	NBP1-44861MFV450
mFluor Violet 500 SE	NBP1-44861MFV500
mFluor Violet 610 SE	NBP1-44861MFV610

compare

(select up to 3 products from this page to compare)

NB100-56588

TLR7 Antibody - BSA Free

(13 Publications)

0.1 mg

NB100-56588

Immunohistochemistry-Paraffin: TLR7 Antibody [NB100-56588] - Analysis of FFPE human brain using TLR7 antibody at 1:20 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Neuronal staining of TLR7 was observed. Staining was performed by Histowiz.

Flow (Intracellular): TLR7 Antibody [NB100-56588] - Analysis using the FITC conjugate of NB100-56588. Staining of TLR7 in TLR7/HEK 293 cells using this antibody at 2 ug/10^6 cells. Green: Vector/HEK 293 cells. Red: TLR7/HEK 293 cells.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-665A

Alt Catalog# IMG-665A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56588AF350
Alexa Fluor 405	NB100-56588AF405
Alexa Fluor 488	NB100-56588AF488
Alexa Fluor 532	NB100-56588AF532
Alexa Fluor 594	NB100-56588AF594
Alexa Fluor 647	NB100-56588AF647
Alexa Fluor 700	NB100-56588AF700
Alexa Fluor 750	NB100-56588AF750
Allophycocyanin	NB100-56588APC
Biotin	NB100-56588B
CoraFluor 1	NB100-56588CL1
DyLight 350	NB100-56588UV
DyLight 405	NB100-56588V
DyLight 488	NB100-56588G
DyLight 550	NB100-56588R
DyLight 594	NB100-56588DL594
DyLight 650	NB100-56588C
DyLight 680	NB100-56588FR
DyLight 755	NB100-56588IR
FITC	NB100-56588F
HRP	NB100-56588H
Janelia Fluor 525	NB100-56588JF525
Janelia Fluor 549	NB100-56588JF549
Janelia Fluor 646	NB100-56588JF646
Janelia Fluor 669	NB100-56588JF669
PE	NB100-56588PE
PerCP	NB100-56588PCP
mFluor Violet 450 SE	NB100-56588MFV450
mFluor Violet 500 SE	NB100-56588MFV500
mFluor Violet 610 SE	NB100-56588MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56588SS

compare

(select up to 3 products from this page to compare)

NB100-56682

TLR7 Antibody - BSA Free

(8 Publications)

0.1 ml

NB100-56682

Immunohistochemistry: TLR7 Antibody [NB100-56682] - Epithelial cells from primary HNEC (B), Detroit-562 (H) and FaDu (N) were incubated with antibody against TLR7 and visualized by 3, 3-diaminobenzidine (brown). In controls, N-series universal negative control reagent was used (F, L, R). All cells were counterstained with haematoxylin (blue). The figure shows one representative staining out of three independent experiments. The markers in the figure are 50 um. The arrows indicate positive stained cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0098239) licensed under a CC-BY license.

Western Blot: TLR7 Antibody [NB100-56682] - Analysis of TLR7 in Ramos cell lysate using NB100-56682 at 1:500.

Immunohistochemistry-Paraffin: TLR7 Antibody [NB100-56682] - Analysis of TLR7 in formalin-fixed, paraffin-embedded human lung tissue using an isotype control (top) and this antibody (bottom) at 1:100.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-540

Alt Catalog# IMG-540

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56682SS

compare

(select up to 3 products from this page to compare)

NBP1-77039

CD81 Antibody - BSA Free

(9 Publications)

0.1 mg

NBP1-77039

Immunocytochemistry/ Immunofluorescence: CD81 Antibody - BSA Free [NBP1-77039] - Immunofluorescence of CD81 in human brain tissue with CD81 antibody at 10 u/ml.Green: CD81 Antibody Blue: DAPI staining

Immunocytochemistry/ Immunofluorescence: CD81 Antibody - BSA Free [NBP1-77039] - Immunofluorescence of CD81 in A549 cells with CD81 antibody at 20 ug/mL.

Immunohistochemistry: CD81 Antibody - BSA Free [NBP1-77039] - Immunohistochemistry of CD81 in human brain tissue with CD81 antibody at 2 ug/ml.

Blood plasma sEV from high fertile and low-fertile both confirmed sEV characteristics and differ in total protein concentrations. (A) Total protein content of sEV from low fertile dairy cow plasma are significantly higher than the high fertile. Values are presented as mean +/- SD (**P 0.05; Mann–Whitney test). (E) Spherical shape was confirmed in electron micrographs of exosomes from both (i) HF-EXO and (ii) LF-EXO. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37012302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Hebp1 is delivered by MCP-EVs and contributes to nerve regeneration. (A) Representative transmission electron microscopy (TEM) phase images for detecting isolated MCP-EVs. Scale bar = 100 nm. (B) Representative Western blot for Hebp1, EV positive markers (CD63, CD9 and CD81) or EV negative marker (GM130) in MCPs and MCP-EVs. (C, D) Expression of Hebp1 in MCP-EVs infected with lentivirus expressing shHebp1 or shCon at three doses (1 × 103, 5 × 104, and 1 × 104 TU/ml culture medium) for at least 3 days. (E, F) Densitometric quantification of Hebp1 protein bands using an image analyzer. Results are presented as means +/- SEM (n = 4). (G, H) Immunofluorescence staining for NF in mouse MPG tissues exposed to LPS (2.5 μg/ml) and other indicated conditions for 5 days (G). Lengths of NF-positive neurites in MPG tissues, quantified using an image analyzer (H). Results are presented as means +/- SEM (n = 4). Scale bar, 100 um. (I, J) Migration assays of mouse Schwann cells exposed to LPS (2.5 μg/ml) and other indicated conditions for 24 hours (I). Number of migrated cells in the red dotted rectangle, quantified using an image analyzer (J). Results are presented as means +/- SEM (n = 4; **P < 0.01, ***P < 0.001). The relative ratio of the shCon (control) group was defined as 1. MCPs, mouse cavernous pericytes; EVs, extracellular vesicles; LPS, lipopolysaccharide; MPG, major pelvic ganglion; PBS, phosphate-buffered saline; ns, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37324943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77039AF350
Alexa Fluor 405	NBP1-77039AF405
Alexa Fluor 488	NBP1-77039AF488
Alexa Fluor 532	NBP1-77039AF532
Alexa Fluor 594	NBP1-77039AF594
Alexa Fluor 647	NBP1-77039AF647
Alexa Fluor 700	NBP1-77039AF700
Alexa Fluor 750	NBP1-77039AF750
Allophycocyanin	NBP1-77039APC
Biotin	NBP1-77039B
CoraFluor 1	NBP1-77039CL1
DyLight 350	NBP1-77039UV
DyLight 405	NBP1-77039V
DyLight 488	NBP1-77039G
DyLight 550	NBP1-77039R
DyLight 594	NBP1-77039DL594
DyLight 650	NBP1-77039C
DyLight 680	NBP1-77039FR
DyLight 755	NBP1-77039IR
FITC	NBP1-77039F
HRP	NBP1-77039H
Janelia Fluor 525	NBP1-77039JF525
Janelia Fluor 549	NBP1-77039JF549
Janelia Fluor 646	NBP1-77039JF646
Janelia Fluor 669	NBP1-77039JF669
PE	NBP1-77039PE
PerCP	NBP1-77039PCP
mFluor Violet 450 SE	NBP1-77039MFV450
mFluor Violet 500 SE	NBP1-77039MFV500
mFluor Violet 610 SE	NBP1-77039MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77039-0.025mg

compare

(select up to 3 products from this page to compare)

DDX0131P-100

CD40/TNFRSF5 Antibody (mAb89) - Azide and BSA Free

(13 Publications)

0.1 mg

DDX0131P-100

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	DDX0131A488-100

compare

(select up to 3 products from this page to compare)

NBP1-78295

BTK [p Tyr223] Antibody - BSA Free

(6 Publications)

0.1 ml

NBP1-78295

Tolebrutinib treatment alleviates inflammatory response and ferroptosis in microglia. Tolebrutinib treatment alleviates inflammatory response and ferroptosis in microglia. (A) Experimental flow chart. (B) Primary microglia were treated with tolebrutiinb at different concentrations for 4 h, and CCK8 were performed. One-way ANOVA and Tukey post hoc test. n = 6 per group. (C-D) Representative image and quantification of pBTK protein levels in primary microglia. One-way ANOVA and Tukey post hoc test, n = 6 per group. (E) Normalized mRNA levels of genes related to inflammatory response, phagocytosis and ferroptosis were measured. One-way ANOVA and Tukey post hoc test. n = 6 per group Image collected and cropped by CiteAb from the following open publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-024-03187-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-78295AF350
Alexa Fluor 405	NBP1-78295AF405
Alexa Fluor 488	NBP1-78295AF488
Alexa Fluor 532	NBP1-78295AF532
Alexa Fluor 594	NBP1-78295AF594
Alexa Fluor 647	NBP1-78295AF647
Alexa Fluor 700	NBP1-78295AF700
Alexa Fluor 750	NBP1-78295AF750
Allophycocyanin	NBP1-78295APC
Biotin	NBP1-78295B
CoraFluor 1	NBP1-78295CL1
DyLight 350	NBP1-78295UV
DyLight 405	NBP1-78295V
DyLight 488	NBP1-78295G
DyLight 550	NBP1-78295R
DyLight 594	NBP1-78295DL594
DyLight 650	NBP1-78295C
DyLight 680	NBP1-78295FR
DyLight 755	NBP1-78295IR
FITC	NBP1-78295F
HRP	NBP1-78295H
Janelia Fluor 525	NBP1-78295JF525
Janelia Fluor 549	NBP1-78295JF549
Janelia Fluor 646	NBP1-78295JF646
Janelia Fluor 669	NBP1-78295JF669
PE	NBP1-78295PE
PerCP	NBP1-78295PCP
mFluor Violet 450 SE	NBP1-78295MFV450
mFluor Violet 500 SE	NBP1-78295MFV500
mFluor Violet 610 SE	NBP1-78295MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-78295SS

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

TLR9 Antibody (26C593.2) - BSA Free

TLR7 Antibody - BSA Free

BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

CD81 Antibody (1D6) - BSA Free

TLR7 Antibody (4G6) - BSA Free

CD19 Antibody (CB19) - BSA Free

CD81 Antibody (M38) - BSA Free

TLR7 Antibody - BSA Free

TLR7 Antibody - BSA Free

CD81 Antibody - BSA Free

CD40/TNFRSF5 Antibody (mAb89) - Azide and BSA Free

BTK [p Tyr223] Antibody - BSA Free