| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-534SS | |||
| Azide and BSA Free | NBP2-80947 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1327SS | |||
| Azide and BSA Free | NBP2-80626 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22114SS | |||
| Azide and BSA Free | NBP2-80827 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-985-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27416 | |||
| BSA Free | NB100-56712SS | |||
| Azide and BSA Free | NBP2-27416SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65543SS | |||
| Azide and BSA Free | NBP2-80661 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71663SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77913-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64895SS | |||
| Azide and BSA Free | NBP2-80651 | |||
![Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-HSn-7D2-Immunohistochemistry-Frozen-NB600-534-img0003.jpg "Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.")
![Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-HSn-7D2-Flow-Cytometry-NB600-534-img0002.jpg)
![Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Sialoadhesin-Antibody-HSn-7D2-Immunohistochemistry-Paraffin-NB600-534-img0001.jpg)
![Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunohistochemistry-NB600-1327-img0012.jpg "Immunohistochemistry: CD11b Antibody (M1/70.15) [NB600-1327] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody.")
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Characterization of microglia cultures by multiple staining with CD11b Antibody (M1/70.15) [NB600-1327] (panels a. & c.) and anti GFAP (panels b & d). Nuclei appear blue due to 4',6-diamidino-2-phenylindole (DAPI) counter staining.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0013.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - A surface stain was performed on Raw264.7 cells with CD11b (M1/70.15) Antibody [NB600-1327R] (blue) and a matched isotype control (orange), both conjugated to DyLight 550. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] (NB600-1327) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1327-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 488 (NB600-1327AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1327-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemistry/Immunofluorescence (ICC/IF) analysis of RAW264.7 cells using CD11b antibody (M1/70.15) [NB600-1327] with Dylight 488 conjugated secondary (green). Nuclei and tubulin were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent staining in untreated and treated mouse microglia cells using CD11b Antibody (M1/70.15) [NB600-1327]. Image from a verified customer review.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0015.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) [NB600-1327] - Immunocytochemical/Immunofluorescent analysis in mouse primary microglia. Cells were treated with lysophosphatidic acid (LPA) or LPA and an LPAR5 inhibitor in order to investigate the expression of iNOS after 24 hours. CD11b Antibody (M1/70.15) [NB600-1327] was used in a dilution 1:100 in order to stain primary murine microglia. Cy2 (green) and Cy3 (red) were used as secondary antibodies.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Immunocytochemistry-Immunofluorescence-NB600-1327-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - BSA Free [NB600-1327] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody [M1/70.15] conjugated to Alexa Fluor 647 (NB600-1327AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1327-img0019.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327].](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0004.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]: RPE - Alexa Fluor 750](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0007.jpg)
![Flow Cytometry: CD11b Antibody (M1/70.15) [NB600-1327] - Staining of mouse peritoneal macrophages using the Alexa Fluor (R) 488 conjugate of CD11b Antibody (M1/70.15) [NB600-1327AF488].](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Flow-Cytometry-NB600-1327-img0014.jpg)
![Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Western-Blot-NBP2-22114-img0002.jpg "Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)")
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lymphoid tissue tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0005.jpg)
![Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Flow cytometric analysis of A549 cells using LAP (TGF-beta 1) mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Flow-Cytometry-NBP2-22114-img0003.jpg)
![Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis of human stomach tissue (A) and human small intestine tissue (B) using TGF beta 1 antibody (NBP2-22114) at 2 ug/ml.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Western-Blot-NBP2-22114-img0007.jpg)
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Human tonsil. Image from verified customer review.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0006.jpg)
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0004.jpg)
![ELISA: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-ELISA-NBP2-22114-img0001.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) [NB600-985] - Cynomolgus monkey spleen. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Immunohistochemistry-Paraffin-NB600-985-img0012.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) [NB600-985] - Cynomolgus monkey spleen. IHC-P image submitted by a verified customer review.")
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0008.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0009.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0010.jpg)
![Immunohistochemistry-Frozen: CD68/SR-D1 Antibody (ED1) [NB600-985] - The feline macrophage containing section was incubated with the antibody at 1:50 and the section was further stained with Alexa secondary antibody. Image was captured with an epifluorescence microscope. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Immunohistochemistry-Frozen-NB600-985-img0015.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Decidual Macrophages were analyzed with a BD LSRFortessa. Antibody was diluted 1:100 in staining buffer before use. Isotype control is in Red. Sample with NB600-985AF700 is in Blue. Image from the Alexa Fluor 700 version of this antibody.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0016.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat liver](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Immunohistochemistry-Paraffin-NB600-985-img0011.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0007.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0013.jpg)
![Flow (Intracellular): CD68/SR-D1 Antibody (ED1) [NB600-985] - An intracellular stain was performed on U-937 cells with CD68/SR-D1 Antibody (ED1) NB600-985PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Intracellular-NB600-985-img0014.jpg)
![Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Western blot analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC-1, VCAM-1 (NBP1-28404), and ICAM-1 (NBP2-22541). GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01767/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Western-Blot-NBP2-22541-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Western blot analysis of MDA-MB-231 cells transfected with/without an IL-8 silencer RNA (siIL-8) to evaluate the expression of MUC-1, VCAM-1 (NBP1-28404), and ICAM-1 (NBP2-22541). GAPDH load control was reused for illustrative purposes. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01767/full), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Frozen: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of frozen rat spleen with Mouse anti Rat CD54.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-Frozen-NBP2-22541-img0011.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in C6 cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0014.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse kidney tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-Paraffin-NBP2-22541-img0001.jpg)
![Immunohistochemistry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse lung tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-NBP2-22541-img0002.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse spleen tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Immunohistochemistry-Paraffin-NBP2-22541-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of stimulated rat spleen cells with mouse anti-rat CD54 (Alexa488).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0010.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Ramos cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0012.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Raji cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1A29-Flow-Cytometry-NBP2-22541-img0013.jpg)
![Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normalized gene & protein expression of ICAM-1. (A) qPCR results indicated no change in mRNA expression of ICAM-1 at 24 h post-overpressure but a significant increase at 48 h as compared to sham. (B) Subsequent Western blot results confirmed elevated ICAM-1 protein levels at 48 h compared to sham. *p < 0.05, Data are represented as mean ± SEM, gene: n = 7–9/group, protein: n = 8–9/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30853931), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22541_mouse-monoclonal-icam-1-cd54-antibody-1a29-31020241536370.jpg)
![Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Fractionated, compared to single exposure, radiation more profoundly enhanced ICAM-1 expression. Representative (3 independent experiments) Western blot analysis & quantification of ICAM-1 levels in whole-cell lysates from nonirradiated (sham) & irradiated HUVECs 4 h & 24 h after exposure to (a) either five fractions of 2 Gy (5 × 2 Gy) or single exposure to 10 Gy & (b) either five fractions of 2.5 Gy (5 × 2.5 Gy) or single exposure to 12.5 Gy. Fractions delivered at 24-h intervals. beta -actin served as a loading control. c Ectopic expression of ICAM-1 after exposure to 0 Gy, five fractions of 2 Gy, or 10 Gy, as measured by flow cytometry (n = 3). d NF-kappa B activation after 4 h of exposure to 0 Gy, five fractions of 2 Gy, or 10 Gy (n = 2). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22541_mouse-monoclonal-icam-1-cd54-antibody-1a29-31020241533490.jpg)
![Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Fractionated thoracic irradiation suppressed KLF2 & enhanced ICAM-1 levels in the lung. Quantification of KLF2 (a) & ICAM-1 (b) protein levels & representative Western blot analysis (c) in the lung tissue of mice (n = 6) at 24 h following 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. beta -actin served as a loading control. KLF2 immunostaining in the lung tissue samples of sham irradiated (d), irradiated (e), & quantitation (f) at 24 h after exposure to 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. ICAM-1 immunostaining in the lung tissue samples of sham irradiated (g), or irradiated (h), & quantitation (i) at 24 h after exposure to 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. (n, number of independent experiments performed; a, significant statistical difference between nonirradiated & irradiated groups; b, significant statistical difference between fractionated irradiation & single exposure; *p < 0.05; **p < 0.01; ***p < 0.001). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22541_mouse-monoclonal-icam-1-cd54-antibody-1a29-31020241611356.jpg)
![Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of P65 using p65 antibody at 2 ug/mL in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Western-Blot-NB100-56712-img0013.jpg "Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of P65 using p65 antibody at 2 ug/mL in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.")
![Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Lane view shows a specific band for NFkB p65 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Simple-Western-NB100-56712-img0006.jpg)
![Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/mL mouse anti-RelA/NFkB p65 monoclonal (NB100-56712, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Western-Blot-NB100-56712-img0016.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/mL. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Immunohistochemistry-Paraffin-NB100-56712-img0009.jpg)
![Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. This NFkB p65 antibody was used for this test with an anti-mouse IgG PE conjugated secondary antibody.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Flow-Cytometry-NB100-56712-img0004.jpg)
![Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Detection of p65 by Simple Western (JESS) in chemiluminescence in nuclear extract of reconstructed human epidermis lysate (about 600 ug/mL protein concentration).Antibody at 1:20. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-112A1021-Simple-Western-NB100-56712-img0017.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-OX-7-Flow-Cytometry-NB100-65543-img0006.jpg "Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus.")
![Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus with MOUSE ANTI RAT CD90 (THY 1.1).](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-OX-7-Flow-Cytometry-NB100-65543-img0001.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-OX-7-Flow-Cytometry-NB100-65543-img0002.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-OX-7-Flow-Cytometry-NB100-65543-img0003.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus cell.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-OX-7-Flow-Cytometry-NB100-65543-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - Staining of mouse glioma tissue using CD31/PECAM-1 Antibody (NBP1-71663) at a dilution of 1:2500. Image provided by Dr. Eric Woolf of Dignity Health.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71663-img0009.jpg "Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - Staining of mouse glioma tissue using CD31/PECAM-1 Antibody (NBP1-71663) at a dilution of 1:2500. Image provided by Dr. Eric Woolf of Dignity Health.")
![Simple Western: CD31/PECAM-1 Antibody [NBP1-71663] - Image shows a specific band for CD31/PECAM 1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Simple-Western-NBP1-71663-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: CD31/PECAM-1 Antibody [NBP1-71663] - 5-HTR1A agonist enhances neovascularization during skin wound healing (excisional punch biopsy model). CD31/PECAM-1 endothelial cell immunohistochemical staining at day 5 (A,B) and day 7 (E,F), image scale bars represented at 50 um. Results were presented as mean +/- SEM (n = 5 images per wound). Two-way ANOVA was performed and significance levels were set at *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphar.2018.01406/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Immunohistochemistry-NBP1-71663-img0012.jpg)
![Western Blot: CD31/PECAM-1 Antibody [NBP1-71663] - Snail and EndMT-related protein expression in lungs in orthotopic murine 4T1 breast cancer model. ag Expression of Snail (see Methods). Levels of CD31/PECAM-1 determined by western blot analysis in pooled (n = 6) samples in control and 4T1 breast cancer-bearing mice from the 1st to 5th week after 4T1 cancer cell inoculation (see Methods). Results presented as fold change vs control sample corresponding to healthy mice. Total protein after transfer was used as loading control Image collected and cropped by Citeab from the following publication (Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice. Breast Cancer Res (2018) licensed under a CC-BY license](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Western-Blot-NBP1-71663-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71663-img0011.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody [NBP1-71663] - CD31/PECAM-1 Antibody was tested in WeHi cells with Dylight 488 (green) alongside a matched isotype control (black).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Flow-Cytometry-NBP1-71663-img0005.jpg)
![Western Blot: CD31/PECAM-1 Antibody [NBP1-71663] - Detection of CD31 in HUVEC cell lysates.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Western-Blot-NBP1-71663-img0008.jpg)
![Blocking/Neutralizing: RelA/NFkB p65 [p Ser276] Inhibitor Peptide Set [NBP2-26505] - Effect of various p65-inhibitor peptides on binding of purified recombinant p65 protein to the DNA in vitro. Recombinant p65 protein (100 ng/sample) was incubated for 30 minutes with 50uM of various peptides in 0.025 ml and then assayed for DNA binding activity by EMSA (Please refer to Takada et al, 2004 for further details).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Inhibitor-Peptide-Set-Blocking-Neutralizing-NBP2-26505-img0008.jpg "Blocking/Neutralizing: RelA/NFkB p65 [p Ser276] Inhibitor Peptide Set [NBP2-26505] - Effect of various p65-inhibitor peptides on binding of purified recombinant p65 protein to the DNA in vitro. Recombinant p65 protein (100 ng/sample) was incubated for 30 minutes with 50uM of various peptides in 0.025 ml and then assayed for DNA binding activity by EMSA (Please refer to Takada et al, 2004 for further details).")
![Blocking/Neutralizing: RelA/NFkB p65 [p Ser276] Inhibitor Peptide Set [NBP2-26505] - PTD-p65-P1 peptide inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30 min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer to Takada et al, 2004 for further details).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Inhibitor-Peptide-Set-Blocking-Neutralizing-NBP2-26505-img0009.jpg)
![Functional (Inhibition): RelA/NFkB p65 [p Ser276] Inhibitor Peptide Set [NBP2-26505] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-26505) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-p-Ser276-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-26505-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD63 Antibody (MEM-259) [NB100-77913] - Human HeLa cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Immunocytochemistry-Immunofluorescence-NB100-77913-img0015.jpg "Immunocytochemistry/Immunofluorescence: CD63 Antibody (MEM-259) [NB100-77913] - Human HeLa cells. ICC/IF image submitted by a verified customer review.")
![Western Blot: CD63 Antibody (MEM-259) [NB100-77913] - Immunoblot analysis of CD63, CD81, TSG101, and CD9 protein in LSC-Exo and MSC-Exo. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-14344-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Western-Blot-NB100-77913-img0016.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (MEM-259) [NB100-77913] - Staining of human spleen (paraffin sections) using anti-CD63 (MEM-259).](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Immunohistochemistry-Paraffin-NB100-77913-img0018.jpg)
![Flow Cytometry: CD63 Antibody (MEM-259) [NB100-77913] - Flow Cytometry: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) PE-CyTM5. Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Flow-Cytometry-NB100-77913-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: CD63 Antibody (MEM-259) [NB100-77913] - Staining of human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Immunocytochemistry-Immunofluorescence-NB100-77913-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD63 Antibody (MEM-259) [NB100-77913] - Staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Immunocytochemistry-Immunofluorescence-NB100-77913-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD63 Antibody (MEM-259) [NB100-77913] - Staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Immunocytochemistry-Immunofluorescence-NB100-77913-img0003.jpg)
![Flow Cytometry: CD63 Antibody (MEM-259) [NB100-77913] - Analysis using the FITC conjugate of NB100-77913. Staining of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 (MEM-259) FITC.](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Flow-Cytometry-NB100-77913-img0006.jpg)
![Flow Cytometry: CD63 Antibody (MEM-259) [NB100-77913] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) PE.](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Flow-Cytometry-NB100-77913-img0009.jpg)
![Flow Cytometry: CD63 Antibody (MEM-259) [NB100-77913] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC.](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Flow-Cytometry-NB100-77913-img0010.jpg)
![Flow Cytometry: CD63 Antibody (MEM-259) [NB100-77913] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Flow-Cytometry-NB100-77913-img0011.jpg)
![Flow Cytometry: CD63 Antibody (MEM-259) [NB100-77913] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) PerCP.](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Flow-Cytometry-NB100-77913-img0012.jpg)
![Flow Cytometry: CD63 Antibody (MEM-259) [NB100-77913] - Flow Cytometry: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) Alexa Fluor® 488. Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/CD63-Antibody-MEM-259-Flow-Cytometry-NB100-77913-img0013.jpg)
![Western Blot: CD63 Antibody (MEM-259) - BSA Free [NB100-77913] - Cytokine & checkpoint molecule arrays from plasma exosomes showed a decreased concentration of IFN-gamma , IL-10, IL-13, B7-1, B7-2, & ICOSL in GBM patients in comparison to normal donors. (A) Quantification of IFN-gamma , IL-10, & IL-13 in plasma exosomes from normal donors & grade 4 GBM patients' plasma exosomes (mean ± SEM, n = 4/group, *P < 0.05). (B) Quantification of B7-1, B7-2, & ICOSL in plasma exosomes from normal donors & grade 4 GBM patients' plasma exosomes (mean ± SEM, n = 4/group). (C) Quantification of the immunosuppressive checkpoint & T cell costimulatory homolog protein PD-L1 in plasma exosomes from normal donors & grade 4 GBM patients' plasma exosomes (mean ± SEM, n = 4/group). Interestingly, PD-L1 is found at similar levels in both normal donor & glioma patient plasma exosomes & these findings were confirmed by western blot. Western Blot analysis also shows that exosomal markers Flotillin-1 & CD63 are found universally in plasma exosomes from normal donors (n = 3), glioblastoma (grade 4) patients (n = 4), & grade 3 glioma patients (n = 3), though CD63 expression may be slightly reduced in glioma patients. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31380286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-77913_mouse-monoclonal-cd63-antibody-mem-259-310202415532072.jpg)
![In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB as shown by EMSA. U266 cells were treated with 100 uM of control or NBD peptide for different time periods. Nuclear extracts were isolated and checked for NF-kB-DNA binding activity.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-In-vivo-assay-NBP2-26504-img0010.jpg "In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB as shown by EMSA. U266 cells were treated with 100 uM of control or NBD peptide for different time periods. Nuclear extracts were isolated and checked for NF-kB-DNA binding activity.")
![In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB activation in human multiple myeloma cells. U266 cells were treated with 100 uM of control (A & B) or NBD peptide (C & D) for 12 hr, cytospun, plated on glass slides, air dried for 1 hr at room temperature and fixed with cold acetone. Slides were blocked with 5% normal goat serum for 1 hr and then incubated with rabbit polyclonal anti-human p65 antibody (A & C) followed by Ig-Alexa 594 second step. In control peptide treated cells, p65 translocates to nucleus (A), whereas NBD peptide prevents translocation of p65 into the nucleus (C). B & D: Nuclear staining with DNA binding dye.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-In-vivo-assay-NBP2-26504-img0012.jpg)
![IKK gamma Inhibitor Peptide Set [NBP2-26504] - The IKK complex consists of IKKalpha + IKKbeta+ NEMO. NEMO (IKKgamma/NF-kB Essential MOdulator) is a scaffold protein required for the kinase activity of IKKalpha + IKKbeta. NBD (NEMO Binding Domain) inhibits formation of the IKK complex (IKKalpha + IKKbeta+ NEMO) by binding to NEMO and blocking IKK complex formation. In this regard, NBD is considered to be an 'IKKalpha and IKKBeta decoy' Blocking IKK complex formation suppresses downstream events in the NF-kB signaling pathway that rely on the IKK complex including phosphorylation of IkBalpha, and subsequence IkBalpha ubiquination, degradation and release of NF-kB. Hence, NF-kB is retained in the cytoplasm in its inactive state.](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-NBP2-26504-img0014.jpg)
![Functional (Inhibition): IKK gamma Inhibitor Peptide Set [NBP2-26504] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-26504) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/IKK-gamma-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-26504-img0016.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0005.jpg "Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using the FITC conjugate of CD45 antibody (OX-1). Staining of rat spleen cells.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0004.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) - BSA Free [NB100-64895] - Representative sequential gating during flow cytometry analysis of rat non-parenchymal liver cells (n = 4). Cells were labeled with antibodies to SE-1 (NB110-68095AF488), CD31, and CD45 (NB100-64895PE). LSECs were then identified as SE-1-Alexa488+ cells (e), and the biexponential CD45-PE/CD31-APC of events (f) were used to display and select the CD45+ CD31+ subsets of LSECs. FMOs (used for gating), and single antibody staining controls are shown in Additional file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-BSA-Free-Flow-Cytometry-NB100-64895-img0006.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Staining of rat peripheral blood lymphocytes with mouse anti-rat CD45 antibody (OX-1) [NB100-64895].](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0001.jpg)