| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24865 | |||
| Azide and BSA Free | NBP2-24865SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27369SS | |||
| Azide and BSA Free | NBP2-80862 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24821SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24827SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24538SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56579SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84682-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NB600-1443-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89546-25ul | |||
![Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 antibody, anti-rat APC conjugated secondary antibody and cell surface flow kit. Green: isotype control. Red: TLR4 antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cell-Surface-NB100-56560-img0010.jpg "Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 antibody, anti-rat APC conjugated secondary antibody and cell surface flow kit. Green: isotype control. Red: TLR4 antibody.")
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 in mouse peritoneal cells. Green: isotype control. Red: anti-TLR4 antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cytometry-NB100-56560-img0009.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram: Cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cytometry-NB100-56560-img0012.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of cell surface TLR4 using this antibody at 0.625 ug/10^6 Raw cells (pretreated with anti-CD16/32). Shaded histogram: isotype control.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cytometry-NB100-56560-img0011.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/nb100-56560_rat-monoclonal-tlr4-antibody-mts510-17720249362619.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in RAW cells using 0.5 ug of this antibody. Black histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/nb100-56560_rat-monoclonal-tlr4-antibody-mts510-17720241003224.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/nb100-56560_rat-monoclonal-tlr4-antibody-mts510-17720241003220.jpg)
![Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Western-Blot-NBP2-27369-img0005.jpg "Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: MyD88 Antibody (4D6) [NBP2-27369] - Analysis of MyD88 antibody in MCF-7 cells using an isotype control (top) and MyD88 Antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Immunocytochemistry-Immunofluorescence-NBP2-27369-img0002.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on Jurkat cells with MyD88 Antibody (4D6) NBP2-27369PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0012.jpg)
![Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Analysis of MyD88 in A) human ovary, B) human prostate and Jurkat cell lysate in the C) absence and D) presence of immunizing peptide using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Western-Blot-NBP2-27369-img0001.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - Intracellular analysis of MyD88 antibody in Jurkat cells using 0.5 ug. Shaded histogram represents cells without antibody; green represents isotype control; red represents MyD88 antibody. This antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Cytometry-NBP2-27369-img0003.jpg)
![Flow (Intracellular): MyD88 Antibody (4D6) [NBP2-27369] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-27369. Staining of MyD88 antibody in Jurkat cells using 10 ul (0.5 ug) of MyD88 antibody. Green represents isotype control (20108); red represents anti-MyD88 antibody.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Intracellular-NBP2-27369-img0006.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 (4D6) antibody NBP2-27369PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Cytometry-NBP2-27369-img0007.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 [4D6] Antibody NBP2-27369AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Cytometry-NBP2-27369-img0008.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on Jurkat cells with MyD88 Antibody (4D6) NBP2-27369 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0009.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on U-937 cells with MyD88 Antibody (4D6) NBP2-27369 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0010.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 Antibody (4D6) NBP2-27369F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0011.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0012.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.")
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Staining of mouse carotid artery. A) No Ab staining of WT uninjured Ms. B) IL-1 beta staining of cells after surgical injury of tissue. C) No Ab staining of injured tissue from an IL-1 beta KO.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42767-img0004.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis of Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45C RT](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-42767-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the Biotin conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0008.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1Beta precursor molecule. Denatured IL-1Beta will have a 18 kDa band.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0011.jpg)
![Western Blot: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 using TLR4 antibody at 2 ug/mL on partial recombinant mouse TLR4 protein.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP2-24821-img0001.jpg "Western Blot: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 using TLR4 antibody at 2 ug/mL on partial recombinant mouse TLR4 protein.")
![Immunocytochemistry/Immunofluorescence: TLR4 Antibody [NBP2-24821] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-TLR4 [NBP2-24821] at a 1:50 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24821-img0006.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 in FFPE (A) normal and (B) tumor prostate sections using TLR4 antibody at 1:50 dilution. ICC analysis of TLR4 in formalin-fixed (C) Ramos and (D) 293 cells using antibody at 1:10 dilution. Data courtesy of Dr. Regina Gandour-Edwards.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-Paraffin-NBP2-24821-img0002.jpg)
![Flow Cytometry: TLR4 Antibody [NBP2-24821] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-24821. Staining of RAW cells were probed using 2 ug of TLR4 antibody (red) and 2 ug of isotype control antibody (green). Novus's TLR cell surface flow kit (10099K) was used for this test.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP2-24821-img0005.jpg)
![Flow Cytometry: TLR4 Antibody [NBP2-24821] - Cell surface analysis using TLR4 antibody. Mouse macrophage cells were probed using 1 ug of TLR4 antibody (red) and 1 ug of isotype control antibody (green). This antibody and PE-conjugated secondary were used for this test.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP2-24821-img0003.jpg)
![Western Blot: TLR5 Antibody [NBP2-24827] - THP-1 cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR5-Antibody-Western-Blot-NBP2-24827-img0004.jpg "Western Blot: TLR5 Antibody [NBP2-24827] - THP-1 cell lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: TLR5 Antibody [NBP2-24827] - Analysis of TLR5 in FFPE human stomach tumor tissue using TLR5 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/TLR5-Antibody-Immunohistochemistry-Paraffin-NBP2-24827-img0002.jpg)
![Flow Cytometry: TLR5 Antibody [NBP2-24827] - Intracellular flow analysis of TLR5 in Balb/c mouse splenocytes using TLR5 antibody at 2 ug/10^6 cells. Shaded histogram: cells without antibody. Green: rabbit IgG isotype control. Red: TLR5 antibody. Goat anti-rabbit PE was used as secondary antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-Flow-Cytometry-NBP2-24827-img0003.jpg)
![Western Blot: TLR4 Antibody [NBP2-24538] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP2-24538-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: TLR4 Antibody [NBP2-24538] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: TLR4 Antibody [NBP2-24538] - The effect of Arg on LPS-induced TLR4, NF-kB mRNA, and protein expression in BMECs. Pure 2nd generation BMECs were starved for 16h and then cultured in DMEM/F12 medium containing 0 or 10ug/mL LPS and 0 or 100ug/mL Arg. After 12h, the mRNA levels of TLR4 and NF-kB were analyzed using qPCR. Protein expression was analyzed using western bolt. b-Actin was used as an internal reference. Data shown are mean +/- SEM of three independent experiments. Means with different letters (A, B, or C) are significantly different (P < 0.05) from each other. Image collected and cropped by CiteAb from the following publication (hindawi.com/journals/mi/2016/9618795/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP2-24538-img0004.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody [NBP2-24538] - Human prostate stained with TLR4 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-Paraffin-NBP2-24538-img0002.jpg)
![Western Blot: TLR4 Antibody [NBP2-24538] - Analysis of TLR4 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide using TLR4 antibody at 0.1 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP2-24538-img0001.jpg)
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0008.jpg "Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in Raw 264.7 cell lysate.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP1-78427-img0003.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse smooth muscle using DAB wtih hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0007.jpg)
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with TLR4 Antibody NBP1-78427AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0009.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0006.jpg)
![Western Blot: TLR4 Antibody [NB100-56579] - Analysis of TLR4 using this antibody on (A) 1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/mL), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/mL on intestine lysates).](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NB100-56579-img0002.jpg "Western Blot: TLR4 Antibody [NB100-56579] - Analysis of TLR4 using this antibody on (A) 1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/mL), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/mL on intestine lysates).")
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0007.jpg "TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.")
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - The TLR4 reporter cell line responds to LPS. The TLR2 / TLR4 inhibitor specifically inhibits TLR4 activation upon LPS stimulation in a dose-response manner.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0008.jpg)
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 /4 Inhibitor suppressed the Pam3CSK4-induced TLR2/TLR1 activity [A] and the LPS-induced TLR4 activity [B] in a dose-response manner, of which IC50 values were measured as 17.2 uM and 10.47 uM, respectively.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0009.jpg)
![Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84682-img0023.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Optineurin Antibody [NBP1-84682] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining shown in green.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84682-img0012.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0016.jpg)
![Western Blot: Optineurin Antibody [NBP1-84682] - Effect of mitochondrial fission protein, Drp1 (FL) and mutant Tau on Optineurin expression in N2a (M) cells. Lanes: M. Ladder M. Ladder 1. N2a cells 2. N2a cells + mutant Tau 3. N2a cells + mutant tau + Drp1 full length + mutant Tau. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84682-img0022.jpg)
![Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682] - Image and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University. 20X and 100X.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Frozen-NBP1-84682-img0006.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0013.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0014.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0015.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in HCa-1 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0003.jpg "Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in HCa-1 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.")
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in HCa-1 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0001.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in MIH-2 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0002.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro.IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-31020241616187.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416182614.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416154619.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416163756.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-89546] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89546-img0011.jpg "Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-89546] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0015.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0012.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0013.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0014.jpg)
![Simple Western: FXR1 Antibody [NBP1-89546] - Simple Western lane view shows a specific band for FXR1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FXR1-Antibody-Simple-Western-NBP1-89546-img0006.jpg)
![Simple Western: FXR1 Antibody [NBP1-89546] - Electropherogram image(s) of corresponding Simple Western lane view. FXR1 antibody was used at 1:100 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/FXR1-Antibody-Simple-Western-NBP1-89546-img0007.jpg)
