| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54790SS | |||
| Azide and BSA Free | NBP2-80644 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-51109 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90364-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24551SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-116SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84682-25ul | |||
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0023.jpg "Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: CD36 Antibody (1283D) [NBP2-54790] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunohistochemistry-Paraffin-NBP2-54790-img0002.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Total protein from human skin and adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-CD36 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD36 Antibody (1283D) [NBP2-54790] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CD36 (1283D) at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective..](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunocytochemistry-Immunofluorescence-NBP2-54790-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - 3T3-L1 adipocytes were pretreated and treated with ML348 (10 uM) for 1 h, followed by BSA or oleate treatment for another 1 h. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD36-Antibody-1283D-BSA-Free-Western-Blot-NBP2-54790-img0024.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-3102024161365.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114783.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416121990.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-31020241612242.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114740.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114741.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - CD36-mediated caveolar endocytosis transports FAs into cells.a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N3-Alexa Fluro 488, & immunostained with anti-CD36 antibody. Colocalization of PacFA & CD36 was quantified from 24 cells over three independent experiments & plotted in (b). The value represents mean ± SEM. c Cells were lysed & subjected to click chemistry assay using N3-biotin. PacFA-labeled proteins were captured with streptavidin beads & subjected to western blot using anti-CD36 & anti-FABP4 antibodies. d WT, Cav1−/−, Cd36−/−, & Cav1−/−;Cd36−/− SVFs were isolated & differentiated into adipocytes & treated with 100 μM 3H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells & subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT & each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416113520.jpg)
![Flow Cytometry: ASGPR1 Antibody (8D7) [NBP1-60150] - Detection of ASGPR1 in HepG2 cells. Red, black and blue line represent the isotype control, cells only, and an ASGPR1 antibody conjugated to FITC with a concentration of 10 ug/ml, respectively.](http://images.novusbio.com/fullsize/ASGPR1-Antibody-8D7-Flow-Cytometry-NBP1-60150-img0001.jpg "Flow Cytometry: ASGPR1 Antibody (8D7) [NBP1-60150] - Detection of ASGPR1 in HepG2 cells. Red, black and blue line represent the isotype control, cells only, and an ASGPR1 antibody conjugated to FITC with a concentration of 10 ug/ml, respectively.")
![Immunohistochemistry-Paraffin: CREB Antibody [NBP1-90364] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP1-90364-img0002.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-90364_rabbit-polyclonal-creb-antibody-3052025726383.jpg)
![Simple Western: CREB Antibody [NBP1-90364] - Human foreskin cell lysate. CREB antibody dilution of 1:50. Protein concentration is 750 ug/mL. Detection is chemiluminescence. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CREB-Antibody-Simple-Western-NBP1-90364-img0008.jpg)
![Simple Western: CREB Antibody [NBP1-90364] - Detection of CREB by Simple Western (JESS) in reconstructed human epidermis lysate (about 600 ug/mL protein concentration). Antibody at 1:50. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CREB-Antibody-Simple-Western-NBP1-90364-img0009.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - ANT-DBS inhibited BDNF-TrkB pathway and its downstream regulator in the hippocampus of epileptic monkeys. Analysis of BDNF (1:1000, NBP1-59304), TrkB (1:500, NPB1-47898), p-ERK, ERK, p-Akt, Akt, p-S6K and S6K by western blotting. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103026) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0009.jpg "Western Blot: BDNF Antibody [NBP1-59304] - ANT-DBS inhibited BDNF-TrkB pathway and its downstream regulator in the hippocampus of epileptic monkeys. Analysis of BDNF (1:1000, NBP1-59304), TrkB (1:500, NPB1-47898), p-ERK, ERK, p-Akt, Akt, p-S6K and S6K by western blotting. Image collected and cropped by CiteAb from the following publication (www.aging-us.com/lookup/doi/10.18632/aging.103026) licensed under a CC-BY license.")
![Immunohistochemistry: BDNF Antibody [NBP1-59304] - Ventral horn region of mouse spinal cord. Concentration 1:200](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-NBP1-59304-img0004.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - ACHN Whole Cell lysates.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0005.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0002.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - ACHN Whole Cell lysates, Antibody Dilution: 1 ug/ml.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0006.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Fetal Liver lysates, Antibody Dilution: 0.5 ug/ml.](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0007.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Sample Tissue: Human ACHN Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/BDNF-Antibody-Western-Blot-NBP1-59304-img0008.jpg)
![Immunohistochemistry: BDNF Antibody [NBP1-59304] - Rhesus macaque spinal cord. Concentration1:300.](http://images.novusbio.com/fullsize/BDNF-Antibody-Immunohistochemistry-NBP1-59304-img0003.jpg)
![Western Blot: BDNF Antibody [NBP1-59304] - Simvastatin treatment increases BDNF expression in primary cortical neurons of AS mice. (A) Primary cultured cortical neurons prepared from wild type & AS embryos were treated with 5 μM simvastatin at DIV14 for 12 h. Neurons were then fixed & processed for double immunofluorescence staining using Ube3a & BDNF antibodies. Representative images were shown. About 12 immunostained neurons in each group were checked for BDNF expression, fluorescence intensity in each cell was quantified & compared. Scale bar, 50 μm. (B) Immunoblot analysis of matured BDNF levels in simvastatin treated primary cortical neurons as described above. (C) Band intensity of the mature BDNF was quantified & normalized to Tuj1 (BDNF/Tuj1). Values are mean ± SD; n = 3. The ‘a’ point P < 0.05 compared to vehicle treated wild type group & “b” denote P < 0.05 with respect to vehicle treated AS group (one way ANOVA with Holm–Sidak post hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31849603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-59304_rabbit-polyclonal-bdnf-antibody-310202416182644.jpg)
![Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/IRF6-Antibody-Western-Blot-NBP1-51911-img0003.jpg "Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).")
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP1-51911-img0006.jpg)
![Western Blot: IRF6 Antibody [NBP1-51911] - (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/IRF6-Antibody-Western-Blot-NBP1-51911-img0001.jpg)
![Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/IRF6-Antibody-Immunohistochemistry-Paraffin-NBP1-51911-img0004.jpg)
![Western Blot: CD81 Antibody [NBP2-20564] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20564_rabbit-polyclonal-cd81-antibody-910202420131279.jpg "Western Blot: CD81 Antibody [NBP2-20564] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CD81 Antibody [NBP2-20564] - CD81 Antibody Immunohistochemical analysis of paraffin-embedded Lung Papillary Adenocarcinoma, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/CD81-Antibody-Immunohistochemistry-Paraffin-NBP2-20564-img0004.jpg)
![Western Blot: CD81 Antibody [NBP2-20564] - AsPC-1 whole cell extracts (5.5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20564_rabbit-polyclonal-cd81-antibody-91020242021979.jpg)
![Western Blot: CD81 Antibody [NBP2-20564] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (NBP2-20564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20564_rabbit-polyclonal-cd81-antibody-91020242011478.jpg)
![Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Western-Blot-NBP1-33661-img0011.jpg "Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-33661] - Analysis of methanol-fixed HeLa, using Syntenin 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33661-img0002.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0005.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0006.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Rat fore brain. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0007.jpg)
![Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse brain.Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:250.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-33661_rabbit-polyclonal-syntenin-1-antibody-immunohistochemistry-frozen-261120251818924.jpg)
![Zebrafish tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33661_rabbit-polyclonal-syntenin-1-antibody-western-blot-261120251836599.jpg)
![Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse brain.Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:250.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min](http://images.novusbio.com/fullsize/nbp1-33661_rabbit-polyclonal-syntenin-1-antibody-immunohistochemistry-frozen-26112025182708.jpg)
![Western Blot: CD74 Antibody [NBP1-33109] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CD74-Antibody-Western-Blot-NBP1-33109-img0007.jpg "Western Blot: CD74 Antibody [NBP1-33109] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CD74 Antibody [NBP1-33109] - Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33109-img0008.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-33109-img0003.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-33109-img0001.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD74-Antibody-Immunohistochemistry-Paraffin-NBP1-33109-img0002.jpg)
![Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp1-33109_rabbit-polyclonal-cd74-antibody-western-blot-1572025113731..png)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (NBP1-33109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33109_rabbit-polyclonal-cd74-antibody-western-blot-261120251848232.jpg)
![Western Blot: c-Maf Antibody [NBP2-24551] - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-cMaf in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NBP2-24551-img0005.jpg "Western Blot: c-Maf Antibody [NBP2-24551] - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-cMaf in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Western Blot: c-Maf Antibody [NBP2-24551] - SUM159 and HCC1954 whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. c-Maf Antibody (NBP2-24551) was used for primary antibody: 1:500, 4C, overnight. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NBP2-24551-img0006.jpg)
![Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human liver cancer using c-MAF antibody at 2ug/ml concentration (1:500 dilution). The primary antibody binding to c-MAF protein was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This c-MAF antibody showed a very strong nuclear and moderate to strong staining in the endothelial cells (blood vessels) and in a subset of apparently infiltrating inflammatory cells. Weak to moderate cytoplasmic and nuclear staining was observed in liver cancer cells and the cells of tumor stroma including fibroblasts.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Immunohistochemistry-Paraffin-NBP2-24551-img0004.jpg)
![Western Blot: c-Maf Antibody [NBP2-24551] - Analysis of Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed at 3 ug/ml.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NBP2-24551-img0001.jpg)
![Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human kidney probed with c-maf antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Immunohistochemistry-Paraffin-NBP2-24551-img0002.jpg)
![Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human placenta probed with c-maf antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Immunohistochemistry-Paraffin-NBP2-24551-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ABCE1 Antibody [NB400-116] - ABCE1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCE1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-116-img0003.jpg "Immunocytochemistry/Immunofluorescence: ABCE1 Antibody [NB400-116] - ABCE1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Western Blot: ABCE1 Antibody [NB400-116] - WB analysis of ABCE1 in A431 lysate.](http://images.novusbio.com/fullsize/ABCE1-Antibody-Western-Blot-NB400-116-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ABCE1 Antibody [NB400-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ABCE1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCE1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-116-img0004.jpg)
![Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone S1. Analysis of PRDX6 expression in MCF-7.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-3A10-2A11-Western-Blot-H00009588-M01-img0002.jpg "Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone S1. Analysis of PRDX6 expression in MCF-7.")
![Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone 3A10-2A11. Analysis of PRDX6 expression in human liver.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-3A10-2A11-Western-Blot-H00009588-M01-img0003.jpg)
![ELISA: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - Detection limit for recombinant GST tagged PRDX6 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-3A10-2A11-Sandwich-ELISA-H00009588-M01-img0001.jpg)
![Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84682-img0023.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Optineurin Antibody [NBP1-84682] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining shown in green.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84682-img0012.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0016.jpg)
![Western Blot: Optineurin Antibody [NBP1-84682] - Effect of mitochondrial fission protein, Drp1 (FL) and mutant Tau on Optineurin expression in N2a (M) cells. Lanes: M. Ladder M. Ladder 1. N2a cells 2. N2a cells + mutant Tau 3. N2a cells + mutant tau + Drp1 full length + mutant Tau. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84682-img0022.jpg)
![Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682] - Image and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University. 20X and 100X.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Frozen-NBP1-84682-img0006.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0013.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0014.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0015.jpg)
