Syntenin 1 Antibody - BSA Free

Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-33661] - Analysis of methanol-fixed HeLa, using Syntenin 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Frozen: Syntenin 1 Antibody [NBP1-33661] - Frozen Sectioned adult mouse brain. Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term diluted at 1:250. Blue: Fluoroshield with DAPI.

Western Blot: Syntenin 1 Antibody [NBP1-33661] - Whole cell extracts (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Rat fore brain. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Frozen: Syntenin 1 Antibody [NBP1-33661] - Frozen Sectioned adult mouse brain. Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term diluted at 1:250. Blue: Fluoroshield with DAPI.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Syntenin 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Syntenin 1 Antibody - BSA Free (NBP1-33661) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Syntenin 1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Syntenin 1. The exact sequence is proprietary.
• Localization: Cell junction, focal adhesion, adherens junction, Cell membrane, Peripheral membrane protein, Endoplasmic reticulum membrane, Peripheral membrane protein, Nucleus, Melanosome, Cytoplasm, cytosol, cytoskeleton
• Predicted Species: Porcine (100%), Chimpanzee (100%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SDCBP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Syntenin 1 Antibody - BSA Free

• MDA9
• MDA-9
• melanoma differentiation associated protein-9
• Melanoma differentiation-associated protein 9
• Pro-TGF-alpha cytoplasmic domain-interacting protein 18
• Scaffold protein Pbp1
• ST1
• SYCLTACIP18
• syndecan binding protein (syntenin)
• Syndecan-binding protein 1
• syntenin-1

Background

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Syntenin 1 Antibody (NBP1-33661)(3)

Publications using NBP1-33661

• Turner N, Abeysinghe P, Sadowski P, Mitchell M Cross-species proteomic and microRNA comparison of extracellular vesicles in human milk, cow�s milk, and infant formula products: moving towards next generation infant formula products bioRxiv 2023-02-24 (Western Blot, Bovine, Human)
• Yiwei Ai, Chenxu Guo, Marta Garcia-Contreras, Laura S. Sánchez B., Andras Saftics, Oluwapelumi Shodubi, Shankar Raghunandan, Junhao Xu, Shang Jui Tsai, Yi Dong, Rong Li, Tijana Jovanovic-Talisman, Stephen J. Gould Endocytosis blocks the vesicular secretion of exosome marker proteins Science Advances 2024-05-10 [PMID: 38718108]
• Turner NP, Abeysinghe P, Sadowski P, Mitchell MD Omics Analysis of Extracellular Vesicles Recovered from Infant Formula Products and Milk: Towards Personalized Infant Nutrition Molecular nutrition & food research 2023-08-10 [PMID: 37562982] (Western Blot, Bovine, Human)

Bioinformatics

• Gene Symbol: SDCBP