CD74 Antibody - Azide Free

Western Blot: CD74 Antibody [NBP1-33109] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: CD74 Antibody [NBP1-33109] - Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: CD74 Antibody [NBP1-33109] - Human lung cancer. CD74 stained by CD74 antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (NBP1-33109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

CD74 Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit CD74 Antibody - Azide Free (NBP1-33109) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-CD74 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CD74. The exact sequence is proprietary.
• Localization: Membrane, Single-pass type II membrane protein
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD74
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CD74 Antibody - Azide Free

• CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
• CD74 antigen
• CD74 molecule, major histocompatibility complex, class II invariant chain
• CD74
• CLIP
• DHLAG
• DHLAGgamma chain of class II antigens
• HLA class II histocompatibility antigen gamma chain
• HLADG
• HLA-DR antigens-associated invariant chain
• HLA-DR-gamma
• Ia antigen-associated invariant chain
• Ia-associated invariant chain
• Ia-gamma
• Ii
• INVG34
• MHC HLA-DR gamma chain
• p33

Background

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of, or in association with, MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41 kDa, depending on genetic splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD74 Antibody (NBP1-33109)(5)

Publications using NBP1-33109

• Hu Y, Hu Q, Ansari M et Al. Airway-derived emphysema-specific alveolar type II cells exhibit impaired regenerative potential in COPD Eur Respir J 2024-08-15 [PMID: 39147413]
• Wu, L;Zhao, Z;Shin, YJ;Yin, Y;Raju, A;Vaiyapuri, TS;Idzham, K;Son, M;Lee, Y;Sa, JK;Chua, JYH;Unal, B;Zhai, Y;Fan, W;Huang, L;Hu, H;Gunaratne, J;Nam, DH;Jiang, T;Tergaonkar, V; Tumour microenvironment programming by an RNA-RNA-binding protein complex creates a druggable vulnerability in IDH-wild-type glioblastoma Nature cell biology 2024-06-01 [PMID: 38858501]
• Ishihara H, Kariya S, Okano M et al. Expression of macrophage migration inhibitory factor and CD74 in the inner ear and middle ear in lipopolysaccharide-induced otitis media. Acta Otolaryngol. 2016-05-16 [PMID: 27181906] (IF/IHC)
• Uhlenbrock F, van Andel E, Andresen L,Skov S. A conserved WW domain-like motif regulates invariant chain-dependent cell-surface transport of the NKG2D ligand ULBP2. Mol. Immunol. 2015-05-13 [PMID: 25983110] (WB, Human)
• Uhlenbrock F, Hagemann-Jensen M, Kehlet S et al. The NKG2D Ligand ULBP2 Is Specifically Regulated through an Invariant Chain-Dependent Endosomal Pathway. J. Immunol. 2014-08-15 [PMID: 25024379] (WB, Human)
  
  Details:
  CD74 antibody used for WB on Jurkat Tag-9 cells transfected or not with siRNA against CD74 (FIGURE 7D).

Bioinformatics

• Gene Symbol: CD74
• Entrez:
  • 972(Human)
• Uniprot:
  • P04233(Human)