| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-644SS | |||
| Azide and BSA Free | NBP2-80970 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-236SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54153SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-237SS | |||
| Azide and BSA Free | NBP2-80968 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44857 | |||
| Azide and BSA Free | NBP2-34528-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-228SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-481SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56167SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33132 | |||
| IHC-Prediluted | NBP2-44711 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78292SS | |||
![Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunohistochemistry-NB500-644-img0005.jpg "Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Survivin Antibody (8E2) [NB500-644] - Immunocytochemical analysis using Survivin Antibody (8E2) [NB500-644] was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Immunocytochemistry-Immunofluorescence-NB500-644-img0004.jpg)
![Flow (Intracellular): Survivin Antibody (8E2) [NB500-644] - An intracellular stain was performed on Daudi cells with Survivin Antibody (8E2) [NB500-644] (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Survivin-Antibody-8E2-Flow-Intracellular-NB500-644-img0006.jpg)
![Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Western blot analysis using [NB500-236]. Lane 1: Phosphorylated Survivin protein and Lane 2: Non-phosphorylated Survivin protein. Note: theoretical molecular weight of Survivin Antibody: 16 kDa.](http://images.novusbio.com/fullsize/Survivin-[p-Thr34]-Antibody-Western-Blot-NB500-236-img0003.jpg "Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Western blot analysis using [NB500-236]. Lane 1: Phosphorylated Survivin protein and Lane 2: Non-phosphorylated Survivin protein. Note: theoretical molecular weight of Survivin Antibody: 16 kDa.")
![Immunocytochemistry/Immunofluorescence: Survivin [p Thr34] Antibody [NB500-236] - Immunocytochemical analysis using Survivin [p Thr34] Antibody [NB500-236] in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Survivin-[p-Thr34]-Antibody-Immunocytochemistry-Immunofluorescence-NB500-236-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Combination therapy with TiOxNPs and IR suppressed the aggressiveness of xenografts in dissociated MIA PaCa sphere cell-bearing mice. Western blot for expression of Survivin [p Thr34] (NB500-236) in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428310/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-2552023151499.jpg)
![Western Blot: Survivin [p Thr34] Antibody [NB500-236] - Combination therapy with TiOxNPs & IR suppressed the aggressiveness of xenografts in dissociated MIA PaCa sphere cell-bearing mice. A HE staining & IHC analysis of p-H2AX, c-caspase-3, ki67, PCNA, snail/Slug, & vimentin in the indicated groups. Scale bar = 50 μm. n=5. B Western blot for expression of p-H2AX, c-caspase-3, p-survivin, snail/Slug, & vimentin in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. C In vivo apoptosis marker TUNEL assay in xenografts of dissociated MIA PaCa sphere-bearing cells treated with TiOxNPs and/or irradiation. n=5. Data are shown as the mean ± standard deviation. ns, not significant. ****p < 0.0001. Scale bar = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-31020241539717.jpg)
![Survivin [p Thr34] was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-Survivin [p Thr34] Antigen Affinity Purified Polyclonal Antibody (Catalog # NB500-236) at 2.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/antibody/nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-immunocytochemistry-immunofluorescence-2662025104450..jpg)
![Survivin [p Thr34] was detected in immersion fixed NIH-3T3 Mouse fibroblast cell line using Rabbit anti Survivin [p Thr34] Antigen Affinity Purified Polyclonal Antibody (Catalog # NB500-236) at 2.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells in various stages of mitosis were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/antibody/nb500-236_rabbit-polyclonal-survivin-p-thr34-antibody-immunocytochemistry-immunofluorescence-2662025104618..jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood lymphocytes with anti-human CD45 antibody (MEM-28) Alexa Fluor 700.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0021.jpg "Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood lymphocytes with anti-human CD45 antibody (MEM-28) Alexa Fluor 700.")
![Immunocytochemistry/Immunofluorescence: CD45 Antibody (MEM-28) [NB500-319] - staining of human peripheral blood mononuclear cell using anti-human CD45 antibody (MEM-28) (green). DNA visualized by DAPI (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Immunocytochemistry-NB500-319-img0001.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-CyTM5.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0018.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (MEM-28) [NB500-319] - Staining of human small intestine (paraffin-embedded sections) with CD45 antibody (MEM-28), 10 ug/ml.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Immunohistochemistry-Paraffin-NB500-319-img0022.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0007.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) purified, GAM-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0008.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) APC-CyTM7.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0010.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0016.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0017.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-CyTM7.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0019.jpg)
![Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-DyLight (R) 594.](http://images.novusbio.com/fullsize/CD45-Antibody-MEM-28-Flow-Cytometry-NB500-319-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MyoD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0005.jpg "Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: MyoD1 Antibody [NBP1-54153] - Detection of MYOD1 in RH30 cells.](http://images.novusbio.com/fullsize/MyoD1-Antibody-Western-Blot-NBP1-54153-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - Staing of MYOD1 in RD cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MyoD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0003.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody (32.1) [NB500-237] - Survivin expression in human chondrosarcoma. Immunohistochemistry and immunoblot for survivin (red staining) from human chondrosarcoma specimens. Specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining. Image collected and cropped by Citeab from the following publication (The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro. BMC Cancer (2011) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-32-1-Immunohistochemistry-Paraffin-NB500-237-img0004.jpg "Immunohistochemistry-Paraffin: Survivin Antibody (32.1) [NB500-237] - Survivin expression in human chondrosarcoma. Immunohistochemistry and immunoblot for survivin (red staining) from human chondrosarcoma specimens. Specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining. Image collected and cropped by Citeab from the following publication (The antiapoptotic gene survivin is highly expressed in human chondrosarcoma and promotes drug resistance in chondrosarcoma cells in vitro. BMC Cancer (2011) licensed under a CC-BY license.")
![Flow Cytometry: Survivin Antibody (32.1) [NB500-237] - An intracellular stain was performed on HeLa cells with FITC-conjugated Survivin Antibody (32.1) [NB500-237F] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Survivin-Antibody-32-1-Flow-Cytometry-NB500-237-img0003.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody (32.1) [NB500-237] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237].](http://images.novusbio.com/fullsize/Survivin-Antibody-32-1-Immunohistochemistry-Paraffin-NB500-237-img0002.jpg)
![Western Blot: Survivin Antibody (32.1) - BSA Free [NB500-237] - Survivin expression in human chondrosarcoma. Immunohistochemistry & immunoblot for survivin (red staining) from human chondrosarcoma specimens. A: Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein. B: High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable. C & D: Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining. E: Strong survivin signal in a tumor cell displaying a mitotic figure (arrow). F: To verify the expression of survivin in human chondrosarcoma, immunoblots were performed from 3 high grade chondrosarcoma lysates (Patient Nr. 5, 7, 10). As control for the correct molecular weight, in vitro-transcribed & -translated (IVTT) recombinant survivin protein, derived from the full-length human cDNA was loaded. Furthermore, lysates from adult human cartilage served as a negative control. Total protein loaded was 1 μg for recombinant human survivin, 60 μg for chondrosarcoma & cartilage lysates. For A, B & E the polyclonal rabbit anti-survivin antibody AF886 was used. For C & D the monoclonal mouse anti-survivin antibody clone 32.1 was used. Original magnifications: 200× (A & C) & 400× (B & D) & 600× (E). Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-237_mouse-monoclonal-survivin-antibody-32-1-31020241616086.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-228] - SCP1 labeled in mouse pachytene preparation (red), using NB 300-228. CDK2 staining, near teleomeres, is also present (green).](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-228-img0003.jpg "Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-228] - SCP1 labeled in mouse pachytene preparation (red), using NB 300-228. CDK2 staining, near teleomeres, is also present (green).")
![Simple Western: SCP1 Antibody [NB300-228] - Lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/SCP1-Antibody-Simple-Western-NB300-228-img0006.jpg)
![Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.](http://images.novusbio.com/fullsize/BP1-Antibody-Western-Blot-NB100-481-img0004.jpg "Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.")
![Immunohistochemistry-Frozen: BP1 Antibody [NB100-481] - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast tissue.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunohistochemistry-Frozen-NB100-481-img0005.jpg)
![Western Blot: BP1 Antibody [NB100-481] - Effect of BP1 on TNFalpha-induced cell death. Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1766), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BP1-Antibody-Western-Blot-NB100-481-img0007.jpg)
![Immunoprecipitation: BP1 Antibody [NB100-481] - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 (NB100-481) antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50ul of Protein A/G beads.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunoprecipitation-NB100-481-img0006.jpg)
![Western Blot: Survivin Antibody [NB100-56167] - Analysis of Survivin using [NB100-56167]. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombinant human survivin protein was used as a positive control. Bands observed slightly lower than the theoretical molecular weight, 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB100-56167-img0005.jpg "Western Blot: Survivin Antibody [NB100-56167] - Analysis of Survivin using [NB100-56167]. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombinant human survivin protein was used as a positive control. Bands observed slightly lower than the theoretical molecular weight, 16 kDa.")
![Immunohistochemistry: Survivin Antibody [NB100-56167] - Immunohistochemical analysis of Survivin in formalin-fixed paraffin-embedded human prostate and breast cancer using Survivin Antibody [NB100-56167]. A and A1, prostate cancer at low and high magnification, respectively. B and B1, breast cancer. B1 is a high magnification of an area of metastasis. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-NB100-56167-img0002.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56167] - Analysis of Survivin in a formalin-fixed paraffin-embedded human brain tissue microarray using Survivin Antibody [NB100-56167]. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB100-56167-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Immunohistochemistry-Paraffin-NBP2-15184-img0019.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.")
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) orstained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5) (orange).](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Flow-Cytometry-NBP2-15184-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Formalin-fixed, paraffin-embedded human colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5)](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Immunohistochemistry-Paraffin-NBP2-15184-img0016.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis using the Azide and BSA Free version of NBP2-15184. Staining of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Flow-Cytometry-NBP2-15184-img0014.jpg)
![Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Simple-Western-NBP2-15184-img0007.jpg)
![Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Simple-Western-NBP2-15184-img0012.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP1-78292-img0005.jpg "Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB image submitted by a verified customer review.")
![Immunohistochemistry: Notch-1 Antibody [NBP1-78292] - Staining of Notch1 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunohistochemistry-NBP1-78292-img0003.jpg)
![Western Blot: Notch-1 Antibody [NBP1-78292] - Analysis of Cleaved Notch1 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP1-78292-img0002.jpg)
![Simple Western: Notch-1 Antibody [NBP1-78292] - Lane view shows a specific band for Notch-1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Simple-Western-NBP1-78292-img0004.jpg)