Human Cortisol ELISA Kit (Colorimetric)

• Reactivity: Hu
• Applications: ELISA, NULL
• Suitable Sample Type: Saliva
• Standard Curve Range: 0.1 to 30 ng/mL
• Sensitivity: 0.3 ng/mL

Human Cortisol ELISA Kit (Colorimetric) Summary

• Description: Cortisol ELISA Kit is an enzyme immunoassay for the quantitative determination of Cortisol in human saliva.
• Standard Curve Range: 0.1 to 30 ng/mL
• Sensitivity: 0.3 ng/mL
• Assay Type: Quantification
• Inter-Assay: CV% < 8.33%
• Intra-Assay: CV% < 8.27%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Cortisol ELISA Kit (Colorimetric)

• Compound F
• Cortisol
• Hydrocortisone

Background

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Cortisol ELISA Kit (KA1885)(22)

Publications using KA1885

• Psarianos A, Philippou A, Papadopetraki A et al. Cortisol and beta -Endorphin Responses During a Two-Month Exercise Training Program in Patients with an Opioid Use Disorder and on a Substitution Treatment. International Journal of Molecular Sciences 2025-06-13 [PMID: 40507987]
• Bitsika V, Sharpley CF, McMillan ME et al. Background cortisol versus social anxiety as correlates of HPA-axis recovery from stress in boys with Autism Spectrum Disorder. Int J Dev Neurosci 2018-08-27 [PMID: 30165174]
• Sharpley CF, Christie DRH, Bitsika V et al. Comparing a genetic and a psychological factor as correlates of anxiety, depression, and chronic stress in men with prostate cancer. Support Care Cancer 2018-03-30 [PMID: 29603029]
• Sharpley CF, Christie DRH, Bitsika V et al. Limitations in the inverse association between Psychological Resilience and depression in prostate cancer patients experiencing chronic physiological stress. Psychooncology 2017-07-10 [PMID: 28692205]
• Bitsika V, Sharpley CF, Agnew LL et al. Social Motivation is Associated with Elevated Salivary Cortisol in Boys with an ASD. Journal of Developmental and Physical Disabilities 2015-10-26
• Jai A, Kyung K, Seon P et al. Wearable Cortisol Aptasensor for Simple and Rapid Real-Time Monitoring. ACS Sens. 2022-01-07 [PMID: 34995062]
• Jennifer G, Mark S, Yulia G et al. The association between prenatal alcohol consumption and preschool child stress system disturbance. Dev Psychobiol. 2020-10-03 [PMID: 33012000]
• Brun N, van P, Hunting E et al. Polystyrene nanoplastics disrupt glucose metabolism and cortisol levels with a possible link to behavioural changes in larval zebrafish. Commun Biol. 2019-10-18 [PMID: 31646185]
• Sharpley C, Bitsika V, McMillan M et al. Dyadic coping and the cortisol:CRP ratio: How marital stress influences physiological state. Physiol Behav. 2019-08-31 [PMID: 31479683]
• Golub Y, Kuitunen-Paul S, Panaseth K et al. Salivary and hair cortisol as biomarkers of emotional and behavioral symptoms in 6-9 year old children. Physiol Behav. 2019-06-20 [PMID: 31228497]
• Bitsika V, Sharpley CF, Andronicos NM et al. Agreement Between self- vs Parent-Ratings of General Anxiety Disorder Symptoms and Salivary Cortisol in boys with an ASD. J Dev Phys Disabil Apr 1 2015
• Das S, Sengupta S, Pathak K et al. Aggression as an independent entity even in psychosis - The role of cortisol. Psychiatry Res 2018-01-01 [PMID: 29120850] (ELISA, Human)
• Bitsika V, Sharpley CF, Sweeney JA et al. A comparison of age, cognitive, hormonal, symptomatic and mood correlates of Aggression towards Others in boys with ASD. Res Dev Disabil 2017-03-06 [PMID: 28279586]
• Sharpley CF, Christie DR, Bitsika V et al. Neurobiological and psychological evidence of chronic stress in prostate cancer patients. Eur J Cancer Care (Engl) 2017-03-02 [PMID: 28252237]
• Bitsika V, Sharpley CF, Andronicos NM, Agnew LL. What worries parents of a child with Autism? Evidence from a biomarker for chronic stress. Res Dev Disabil 2017-03-01 [PMID: 28202252]
• Sharpley CF, Bitsika V, Andronicos NM, Agnew LL. Further evidence of HPA-axis dysregulation and its correlation with depression in Autism Spectrum Disorders: Data from girls. Physiol Behav 2016-09-13 [PMID: 27619171]
• Kadam PD, Chuan HH. Erratum to: Rectocutaneous fistula with transmigration of the suture: a rare delayed complication of vault fixation with the sacrospinous ligament. Int Urogynecol J. 2016-03-01 [PMID: 26811110]
  
  Details:
  This citation used the HRP version of this antibody.
• Bitsika V, Sharpley CF, Andronicos NM, Agnew LL Prevalence, structure and correlates of anxiety-depression in boys with an autism spectrum disorder. Res Dev Disabil 2016-02-01 [PMID: 26771668]
• Bitsika V, Sharpley CF, Agnew LL, Andronicos NM. Age-related differences in the association between stereotypic behaviour and salivary cortisol in young males with an Autism Spectrum Disorder. Physiol Behav. 2015-10-09 [PMID: 26456519]
• Christopher F, Sharpley, Vicki Bitsikaa, Linda L, Agnew, Nicholas M. Andronicos. Eight-month test-retest agreement in morning salivary cortisol, self- and parent-rated anxiety in boys with an Autism Spectrum Disorder. Physiol Behav. 2015-06-25 [PMID: 26220465]
• Bitsika V, Sharpley CF, Andronicos NM, Agnew LL. Hypothalamus-Pituitary-Adrenal axis daily fluctuation, anxiety and age interact to predict cortisol concentrations in boys with an Autism Spectrum Disorder. Physiology & Behavior. 2014-01-01 [PMID: 25446203]
• Bitsika V, Sharpley CF, Sweeney JA, McFarlane JR. HPA and SAM axis responses as correlates of self- vs parental ratings of anxiety in boys with an Autistic Disorder. Physiol Behav. 2014-03-29 [PMID: 24412722] (Human)

FAQs for Cortisol ELISA Kit (KA1885). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.