| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-6106SS | |||
| Azide and BSA Free | NBP2-80778 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27335 | |||
| BSA Free | NB100-56599SS | |||
| Azide and BSA Free | NBP2-27335SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44038 | |||
| BSA Free | NBP2-59783-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33074 | |||
| IHC-Prediluted | NBP2-44976 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-108SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2080SS | |||
| Azide and BSA Free | NBP2-80733 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-353SS | |||
| Azide and BSA Free | NBP2-80606 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56095SS | |||
![Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0076.jpg "Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: p53 Antibody [NB200-171] - FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p53-Antibody-Immunohistochemistry-Paraffin-NB200-171-img0075.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- anti-KLH control IgG; Red- anti-HDMX.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0002.jpg)
![Chromatin Immunoprecipitation Sequencing: p53 Antibody [NB200-171] - Chromatin from MCF7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53. ChIP-seq validation performed by Active Motif, Carlsbad, CA.](http://images.novusbio.com/fullsize/p53-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB200-171-img0073.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0003.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - Baseline p53 expression in human lymphocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: p53 Antibody [NB200-171] - Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody NB200-171 used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody NB100-2204. For blotting immunoprecipitated P53, NB200-171 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NB200-171-img0077.jpg)
![Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - analysis of hnRNP L in HEK whole cell lysate using anti-hnRNP L antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Western-Blot-NB120-6106-img0005.jpg "Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - analysis of hnRNP L in HEK whole cell lysate using anti-hnRNP L antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: hnRNP L Antibody (4D11) [NB120-6106] - hnRNP L antibody was tested in HeLa cells at 5 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NB120-6106-img0004.jpg)
![Immunohistochemistry-Frozen: hnRNP L Antibody (4D11) [NB120-6106]](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Immunohistochemistry-Frozen-NB120-6106-img0002.jpg)
![Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Western blot for anti-hnRNP-L on HeLa cell extracts](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Western-Blot-NB120-6106-img0001.jpg)
![Western Blot: hnRNP L Antibody (4D11) [NB120-6106] - Whole cell protein from HeLa (lane 1), A431 (lane 2), NIH-3T3 (lane 3) and PC12 (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was probed with Anti-hnRNP L at 1 ug/ml and detected with an HRP conjugated anti-mouse secondary antibody by chemiluminescence.](http://images.novusbio.com/fullsize/hnRNP-L-Antibody-4D11-Western-Blot-NB120-6106-img0003.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Immunocytochemistry-Immunofluorescence-NB100-56599-img0005.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0003.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0016.jpg "Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - The antibody was used to detect RAD18 in U2os Cells. Anti-mouse 2nd antibody was used for detection (Red). DAPI was used for nuclear staining (Blue). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Immunofluorescence-H00056852-M01-img0018.jpg)
![Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Immunohistochemistry-Paraffin-H00056852-M01-img0011.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi ( Cat # H00056852-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody (M01), clone 3H7 (Cat # H00056852-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0012.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7. Analysis of RAD18 expression in PC-12.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0014.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7 Analysis of RAD18 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Immunocytochemistry-Immunofluorescence-H00056852-M01-img0009.jpg)
![ELISA: RAD18 Antibody (3H7) [H00056852-M01] - Detection limit for recombinant GST tagged RAD18 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-ELISA-H00056852-M01-img0017.jpg)
![Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783] - Analysis in human cell line MCF-7.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Western-Blot-NBP2-59783-img0012.jpg "Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783] - Analysis in human cell line MCF-7.")
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human colon shows nuclear membrane positivity in glandular and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human tonsil shows strong nuclear membrane immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human oral mucosa shows nuclear membrane positivity in the epithelial cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human prostate shows nuclear membrane immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human small intestine shows nuclear membrane immunoreactivity in glandular and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0004.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human testis shows strong nuclear membrane positivity in a subset of cells in the seminiferous tubules.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0005.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0010.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0008.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0001.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0003.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0004.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0005.jpg)
![Western Blot: NTH1 Antibody [NB100-108] - Detection of NTH1 (37 kDa) from Hela & K562 cell extracts using NB100-108 (1:500). WB data courtesy of Mark Kelly, Indiana Univ.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-108-img0005.jpg "Western Blot: NTH1 Antibody [NB100-108] - Detection of NTH1 (37 kDa) from Hela & K562 cell extracts using NB100-108 (1:500). WB data courtesy of Mark Kelly, Indiana Univ.")
![Immunocytochemistry/Immunofluorescence: NTH1 Antibody [NB100-108] - NTH1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-108-img0006.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human colon adenocarcinoma using NTH1 antibody at 5ug/ml concentration (1:200 dilution). The primary antibody binding to NTH1/ NTHL1 antigen was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. The NTH1 antibody generated an expected nuclear cytoplasmic staining of NTH1 protein in colon cancer cells as well as the cells of tumor stroma including cancer associated fibroblasts. The nuclear staining of NTH1 was very strong in a sub-set of colon cancer cells.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0014.jpg)
![Western Blot: NTH1 Antibody [NB100-108] - Analysis of NTH1 expression in 1) HeLa, 2) A-431, 3) MCF7 whole cell lysates.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-108-img0002.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lymph node cancer using 5 ug/ml concentration of NTH1 antibody. The representative image shows strong nuclear and cytoplasmic positivity of NTH1 protein in the lymph node cancer cells.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0013.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney cancer using 5 ug/ml concentration of NTH1 antibody. The renal cancer cells showed nuclear and cytoplasmic positivity for NTH1 protein, whereas, the tumor stroma was largely negative for immunostaining.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0012.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. The rectal cancer cells as well as the goblet cells in glandular areas showed nuclear-cytoplasmic positivity for NTH1 protein. The cells of tumor stroma did not develop immunostaining for this protein.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0011.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. This representative image at high resolution depicts nuclear-cytoplasmic positivity for NTH1 protein in rectal cancer cells and the goblet cells. The cells of tumor stroma did not develop any immunostaining for this protein.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] (NB100-2080) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Immunocytochemistry-Immunofluorescence-NB100-2080-img0011.jpg "Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] (NB100-2080) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] conjugated to Alexa Fluor 488 (NB100-2080AF488) at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Immunocytochemistry-Immunofluorescence-NB100-2080-img0012.jpg)
![Western Blot: FGF R1 Antibody (M17D10) [NB100-2080] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Western-Blot-NB100-2080-img0004.jpg)
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/ml concentration. Specific membrane-cytoplasmic immunopositivity of FGF R1 protein was observed in SCC cells whereas the nuclei of SCC cells as well as the tumor stroma was largely negative for this protein.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0008.jpg)
![Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/mL) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Flow-Cytometry-NB100-2080-img0010.jpg)
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human normal skin using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. Cells of the epidermal layer showed a very strong membrane-cytoplasmic staining with relatively weak nuclear immunopositivity for for this protein. The stratum basale / bottom layer of keratinocytes in the epidermis showed a membrane-cytoplasmic stainin for FGF R1.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0006.jpg)
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human liver using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. The hepatocytes depicted an expected and specific membrane-cytoplasmic-nuclear immunostaining of FGF R1 protein.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0007.jpg)
![Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Flow-Cytometry-NB100-2080-img0009.jpg)
![Western Blot: EIF3A Antibody [NBP1-18891] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. eIF3A/EIF3S10 was also immunoprecipitated by rabbit antieIF3A/ EIF3S10 antibody NBP1-18892.](http://images.novusbio.com/fullsize/EIF3A-Antibody-Western-Blot-NBP1-18891-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: EIF3A Antibody [NBP1-18891] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. eIF3A/EIF3S10 was also immunoprecipitated by rabbit antieIF3A/ EIF3S10 antibody NBP1-18892.")
![Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Western-Blot-NB100-353-img0006.jpg "Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Immunocytochemistry-Immunofluorescence-NB100-353-img0005.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0008.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0007.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Total protein from human THP1, A431, A549 and U2OS cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:2000 dilution of anti-BAX in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0006.jpg "Western Blot: Bax Antibody [NB100-56095] - Total protein from human THP1, A431, A549 and U2OS cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:2000 dilution of anti-BAX in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56095] - Formalin-fixed, paraffin-embedded sections of human colon cancer fom a tissue microarray stained for BAX using NB100-56095 at 1:2000. A. High Bax expression. B. Low Bax expression. Hematoxylin-eosin counterstain](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56095-img0007.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Analysis of BAX in colon tumor cell lines using this antibody. Bax is detected in the SW620 and DLD-1 cell lines. However, Bax was not detected in the cell lines (LoVo, DU145, and LS180) known to have frameshift mutations in Bax (Rampino et al. 1997). An antibody to F1ATPase was used as a protein loading control.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0004.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Analysis of Bax in Daoy whole cell lysate using anti-Bax antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0005.jpg)
![Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Podoplanin (NZ-1.2) was detected in immersion fixed paraffin-embedded sections of human appendix using Rat Anti-Human Podoplanin Monoclonal Antibody (Catalog # NBP2-03952) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Immunohistochemistry-Paraffin-NBP2-03952-img0016.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Podoplanin (NZ-1.2) was detected in immersion fixed paraffin-embedded sections of human appendix using Rat Anti-Human Podoplanin Monoclonal Antibody (Catalog # NBP2-03952) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface.")
![Western Blot: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Detection of lysate from human A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Western-Blot-NBP2-03952-img0014.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - A surface stain was performed on Ntera2 cells with Podoplanin Antibody (NZ-1.2) NBP2-03952AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0017.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0009.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0010.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 0.2 ug of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green). Flow kit and anti-rat PE secondary were used for this test.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0012.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the PE conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.2 ug) of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0013.jpg)