| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50599-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-96140SS | |||
| Azide and BSA Free | NBP2-80599 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-109SS | |||
| Azide and BSA Free | NBP2-80882 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73253 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71662SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87757-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61646SS | |||
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/EGFR-Antibody-DH8-3-EGFRvIII-Flow-Cytometry-NBP2-50599-img0002.jpg "Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.")
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/EGFR-Antibody-DH8-3-EGFRvIII-Flow-Cytometry-NBP2-50599-img0001.jpg)
![Flow Cytometry: Mouse Monoclonal EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - Analysis of glioblastoma cell lines using EGFR Antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-50599_mouse-monoclonal-egfr-antibody-dh8-3-egfrviii-mutant-flow-cytometry-145202414346..png)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0008.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0009.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0003.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on NIH3T3 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0012.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0005.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0007.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U937 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0011.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-immunocytochemistry-immunofluorescence-141120251833319.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.")
![Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.](http://images.novusbio.com/fullsize/n-Myc-Antibody-NMYC-1-Western-Blot-NB200-109-img0002.jpg "Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/n-Myc-Antibody-NMYC-1-Immunocytochemistry-Immunofluorescence-NB200-109-img0003.jpg)
![Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in human heart tissue.](http://images.novusbio.com/fullsize/n-Myc-Antibody-NMYC-1-Western-Blot-NB200-109-img0001.jpg)
![Western Blot: c-Myc Antibody [NB600-334] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-c-myc antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-334-img0005.jpg "Western Blot: c-Myc Antibody [NB600-334] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-c-myc antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing expression of CCAR1 and necdin in primary myoblasts isolated from wt, Ndn-/-, tgMlcNec newborn mice. Proteins were detected using antibodies specific for CCAR1, necdin and beta-actin as control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0010.jpg "Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing expression of CCAR1 and necdin in primary myoblasts isolated from wt, Ndn-/-, tgMlcNec newborn mice. Proteins were detected using antibodies specific for CCAR1, necdin and beta-actin as control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: CCAR1 Antibody [NB500-186] - Necdin interacts with CCAR1/CARP1. Co-localization of necdin and CCAR1 in primary myoblasts of wt (C57/Bl6) mice. Images taken at confocal laser scanning microscope at 40x (i-v) and 63x (zoomed 1.67 times) magnification. Nuclei are stained with DAPI (i, vi; blue). Panels shows immunostaining experiment using the polyclonal anti-CCAR1 (ii, vii, Ab-CCAR1-red) or monoclonal anti-Ndn (iii, viii; Ab-Ndn-green). Panel iv shows the merged images of ii and iii and panel ix the merged images of vii and viii (Ab-CCAR1 + Ab-Ndn-yellow). Panel v shows the merged images of i, ii and iii and panel x the merged images of vi, vii, viii, (Ab-CCAR + Ab-Ndn + DAPI). Scale bar (i-iv) = 17 um; (vi-x) = 41,75 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB500-186-img0011.jpg)
![Immunohistochemistry-Paraffin: CCAR1 Antibody [NB500-186] - Human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-CCAR1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunohistochemistry-Paraffin-NB500-186-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CCAR1 Antibody [NB500-186] - Detection of Human CCAR1on HeLa whole cell lysate using NB500-186. CCAR1 was also IPed with another rabbit anti-CCAR1 antibody, NB500-187, and NB500-188. IPed CCAR1 was blotted using NB500-188.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CCAR1 Antibody [NB500-186] - Detection of Human and Mouse CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0006.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin interacts with CCAR1/CARP1. Interaction of necdin and CCAR1 in C2C12 myoblasts and primary myoblast cells. Co-IP experiments were performed on protein extract from C2C12 transfected with pCMV-FLAG-Necdin (C2C12 pCMV-FLAG-Ndn) or primary myoblasts from C57/Bl6 newborn mice. Proteins were immunoprecipitated using the polyclonal anti-Ndn (alpha-Ndn) or polyclonal anti-CCAR1 (alpha-CCAR1) and with a non-specific rabbit antisera as control (IgG). Necdin and CCAR1 were detected in immunoprecipitated samples using specific antibody. Input (I) represents 10% of the immunoprecipitated proteins. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0008.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Necdin controls CCAR1 protein abundance in myogenic cells. Representative western blot showing CCAR1 and necdin (beta-actin as loading control) expression in C2C12 mock transfected (M) or transfected with pSG5-HA-CCAR1 and/or pCMV-FLAG-Necdin (N or N+C). Both the endogenous and transfected CCAR1 were detected with the polyclonal anti-CCAR1, the transfected CCAR1 with anti-HA. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Western-Blot-NB500-186-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CCAR1 Antibody [NB500-186] - Images taken at confocal laser scanning microscope (63x magnif. zoomed 1.67 x) showing co-immunostaining on C2C12 transfected with pSG5-HA-CCAR1 (i) and/or pCMV-Ndn (C2C12, CCAR1-Ndn-N+C) (ii, iii) and on primary myoblasts from Ndn-/- (Ndn-/-) (iv) and tgMlcNec (MlcNec) (v) newborn mice. Panels i-v show immunostaining using the specific monoclonal anti-Ndn (Ndn: Ab-Ndn-green); panels i'-v' show immunostaining using the polyclonal anti-CCAR1 (CCAR1: Ab-CCAR1-red). Co-immunostained images of anti-Ndn and anti-CCAR1 are shown in panels i''-v'' (Ab-CCAR1 + Ab-Ndn-yellow). Panels i'''-v''' show merged images with nuclei stained with DAPI (merge Ab-CCAR1 + ab-Ndn + DAPI). Scale bars (i-iii) 41,75 um; (iv-v) 44,7 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0043335), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunocytochemistry-Immunofluorescence-NB500-186-img0012.jpg)
![Immunoprecipitation: CCAR1 Antibody [NB500-186] - Detection of human CCAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CCAR1 antibody NB500-186 (lot NB500-186-2) used for IP at 6 ug/mg lysate. CCAR1 was also immunoprecipitated by a previous lot (lot NB500-186-1) of the antibody. For blotting immunoprecipitated CCAR1, NB500-186 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCAR1-Antibody-Immunoprecipitation-NB500-186-img0007.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Autoantibody discovery in anti–TIF1-gamma –positive DM patients without cancer.(A) Immunoprecipitations (IPs) were performed using lysates made from radiolabeled cells & plasma from anti–TIF1-gamma –positive DM patients, 5 of whom did not have a cancer, & 5 of whom had a detected cancer. An IP performed with a sample from a healthy control (HC) individual is shown in the right-most lane. Migration of molecular weight standards is marked on the left. (B) Immunoblotted lysates. Lysates made from HeLa & A431 cells were immunoblotted with commercial antibodies against TIF1-gamma & CCAR1, as described in the Methods section. Both proteins migrate at approximately 150 kDa. (C) Interaction between CCAR1 & TIF1-gamma . Co-IPs were performed as described in the Methods section, using antibodies against CCAR1 (upper panel, 2 left lanes) or TIF1-gamma (lower panel, 2 left lanes). Detection of the IPs was performed by immunoblotting with anti–TIF1-gamma (upper panel, 2 left lanes) or anti-CCAR1 (lower panel, 2 left lanes) antibodies. Control IPs, performed using Protein A beads only, were performed & immunoblotted as above. IPs were performed in duplicate. These data are representative of those obtained in 2 additional experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35040440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-186_rabbit-polyclonal-ccar1-antibody-310202416182617.jpg)
![Western Blot: CCAR1 Antibody [NB500-186] - Autoantibody discovery in anti–TIF1-gamma –positive DM patients without cancer.(A) Immunoprecipitations (IPs) were performed using lysates made from radiolabeled cells & plasma from anti–TIF1-gamma –positive DM patients, 5 of whom did not have a cancer, & 5 of whom had a detected cancer. An IP performed with a sample from a healthy control (HC) individual is shown in the right-most lane. Migration of molecular weight standards is marked on the left. (B) Immunoblotted lysates. Lysates made from HeLa & A431 cells were immunoblotted with commercial antibodies against TIF1-gamma & CCAR1, as described in the Methods section. Both proteins migrate at approximately 150 kDa. (C) Interaction between CCAR1 & TIF1-gamma . Co-IPs were performed as described in the Methods section, using antibodies against CCAR1 (upper panel, 2 left lanes) or TIF1-gamma (lower panel, 2 left lanes). Detection of the IPs was performed by immunoblotting with anti–TIF1-gamma (upper panel, 2 left lanes) or anti-CCAR1 (lower panel, 2 left lanes) antibodies. Control IPs, performed using Protein A beads only, were performed & immunoblotted as above. IPs were performed in duplicate. These data are representative of those obtained in 2 additional experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35040440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-186_rabbit-polyclonal-ccar1-antibody-310202416165639.jpg)
![Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Western-Blot-NBP1-47945-img0023.jpg "Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody (OTI5C7) [NBP1-47945] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunocytochemistry-NBP1-47945-img0013.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Human endometrium tissue.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0024.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0014.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0015.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0016.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0017.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0018.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0019.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Patched 1 Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0020.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human Kidney tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0021.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0022.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched 1-PTCH Antibody - BSA Free-Western Blot-NB200-118-img0004.jpg "Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Evaluation of IGF-1, IGF1R and HH pathway expression in fibroblasts and oral squamous carcinoma cells. IGF1 mRNA expression in SCC-4 and MF1 cells, evaluated by RT-qPCR. (C) Evaluation of HH pathway component expression in MF1 by immunocytochemistry. Patched 1/PTCH Antibody (NB200-118); Sonic Hedgehog/Shh Antibody (NBP2-22126). Magnification: 400x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB200-118-img0003.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Increased Ihh mRNA and protein levels and activity in Smad2 and Smad3 mutant growth plates.All images are P0 proximal tibias. Immunostaining for Ptch1, showing increased protein levels throughout the growth plate in mutants, particularly in the resting zone. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006352) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunohistochemistry-NB200-118-img0002.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Western-Blot-NB200-118-img0001.jpg)
![Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Western-Blot-NBP1-71662-img0006.jpg "Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.")
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NBP1-71662-img0005.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NBP1-71662-img0004.jpg)
![Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP1-87757-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: NF2/Merlin Antibody [NBP1-87757] - Whole cell lysates from SUM159, MDA-MB-231 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. NF2/Merlin Antibody (NBP1-87757) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP1-87757-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NF2/Merlin Antibody [NBP1-87757] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87757-img0008.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human fallopian tube shows moderate cytoplasmic and moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0011.jpg)
![Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Western-Blot-NBP1-87757-img0007.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0001.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0009.jpg)
![Immunohistochemistry-Paraffin: NF2/Merlin Antibody [NBP1-87757] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/NF2-Merlin-Antibody-Immunohistochemistry-Paraffin-NBP1-87757-img0010.jpg)
![Western Blot: EGFR Antibody [NBP1-84814] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NBP1-84814-img0009.jpg "Western Blot: EGFR Antibody [NBP1-84814] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP1-84814] - Staining of human cell line A-431 shows positivity in plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84814-img0006.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0017.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0015.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0016.jpg)
![Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Western-Blot-NB110-61646-img0012.jpg "Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.")
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0010.jpg)