Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
161 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-47945 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Patched 1/PTCH Antibody (OTI5c7)
patched (Drosophila) homolog
patched homolog (Drosophila)
patched homolog 1 (Drosophila)
patched homolog 1
protein patched homolog 1
PTCH protein +12b
PTCH protein +4'
PTCH protein -10
This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in umorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus Syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Dias RB. Estudo do perfil de expressao de componentes da via de sinalizacao sonic hedgehog em displasia epitelial oral [Study of the expression of components of sonic hedgehog signaling pathway in oral epithelial dysplasia profile. Thesis: http://arca.icict.fiocruz.br/handle/icict/7483]. Thesis (IHC-P, Human)
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FAQs for Patched 1/PTCH Antibody (NBP1-47945). (Showing 1 - 2 of 2 FAQs).
I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.
Is the target of this antibody (Aminoacid 122-436) on the N-terminal or the C-terminal region? What ist the expected localization of this antibody?
Since we do not map epitope we do not know the exact location but yes, the immunogen is the recombinant protein expressed in E.coli corresponding to amino acids 122-436 of human Patched1 (NP_000255). The protein is 1447 amino acids with a molecular weight of 160, 545 D therefore the immunogen located more towards the N-terminus and not the C-terminus however it is really not at the N-terminus. http://www.uniprot.org/uniprot/Q13635
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