Patched 1/PTCH Antibody (OTI5C7)

Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.

Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody (OTI5C7) [NBP1-47945] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Patched 1.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Human endometrium tissue.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Patched 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Patched 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Patched 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Patched 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Patched 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Patched 1 Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Patched 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human Kidney tissue using anti-Patched 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI5C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Patched 1/PTCH Antibody (OTI5C7) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 122-436 of human PTCH1 (NP_000255) produced in E.coli.
• Specificity: This antibody is specific for Homo sapiens patched homolog 1 (Drosophila) (PTCH1), transcript variant 1b.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTCH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 160.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Patched 1/PTCH Antibody (OTI5C7)

• BCNS
• BCNSFLJ42602
• FLJ26746
• HPE7
• mes
• NBCCS
• patched (Drosophila) homolog
• Patched 1
• patched homolog (Drosophila)
• patched homolog 1 (Drosophila)
• patched homolog 1
• protein patched homolog 1
• PTC
• ptc1
• PTCH protein +12b
• PTCH protein +4'
• PTCH protein -10
• PTCH
• PTCH1
• PTCH11

Background

This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in umorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus Syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Patched 1/PTCH Antibody (NBP1-47945)(4)

Publications using NBP1-47945

• Dias RB. Estudo do perfil de expressao de componentes da via de sinalizacao sonic hedgehog em displasia epitelial oral [Study of the expression of components of sonic hedgehog signaling pathway in oral epithelial dysplasia profile. Thesis: http://arca.icict.fiocruz.br/handle/icict/7483]. Thesis (IHC-P, Human)
• Karin-Kujundzic V, Covarrubias-Pinto A, Skrtic A et al. New insights into the role of PTCH1 protein in serous ovarian carcinomas Research Square 2021-09-09 [PMID: 36205138] (WB, IF/IHC, ICC/IF, Human)
• Hasanovic A, Ruggiero C, Jung S, Rapa I. Targeting the multidrug transporter Patched potentiates chemotherapy efficiency on adrenocortical carcinoma in vitro and in vivo. Int. J. Cancer. 2018-02-07 [PMID: 29411361] (IF/IHC, Human)
• Bolanos AL, Milla CM, Lira JC et al. Role of Sonic Hedgehog in idiopathic pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol. 2012-12-01 [PMID: 23023967] (IF/IHC, Human)
  
  Details:
  Smoothened antibody (IMG 5904A) IHC-P ; Human Lung. (See Fig 3)

FAQs for Patched 1/PTCH Antibody (NBP1-47945). (Showing 1 - 2 of 2 FAQs).

1. I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
   • NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.
2. Is the target of this antibody (Aminoacid 122-436) on the N-terminal or the C-terminal region? What ist the expected localization of this antibody?
   • Since we do not map epitope we do not know the exact location but yes, the immunogen is the recombinant protein expressed in E.coli corresponding to amino acids 122-436 of human Patched1 (NP_000255). The protein is 1447 amino acids with a molecular weight of 160, 545 D therefore the immunogen located more towards the N-terminus and not the C-terminus however it is really not at the N-terminus. http://www.uniprot.org/uniprot/Q13635

Bioinformatics

• Gene Symbol: PTCH1
• Entrez:
  • 5727(Human)
• Uniprot:
  • Q13635(Human)