| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-353SS | |||
| Azide and BSA Free | NBP2-80606 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85729-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90956-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86635-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86185-25ul | |||
![Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Western-Blot-NB100-353-img0006.jpg "Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Immunocytochemistry-Immunofluorescence-NB100-353-img0005.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0008.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0007.jpg)
![Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Western-Blot-NB100-206-img0006.jpg "Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.")
![Immunocytochemistry/Immunofluorescence: SMC1 [p Ser966] Antibody [NB100-206] - Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-206-img0005.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human breast carcinoma. Antibody: Affinity purified rabbit antiPhospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0008.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human testicular seminoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0007.jpg)
![SMC1 [p Ser966] Antibody [NB100-206] - Detection of human Phospho SMC1 (S966) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho SMC1 (S966) antibody NB100-206 used for IP at 3 ul per reaction. Phospho SMC1 (S966) was also immunoprecipitated by rabbit anti-SMC1 antibody NB100-204. For blotting immunoprecipitated Phospho SMC1 NB100-206 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody NB100-204 was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-NB100-206-img0009.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-immunocytochemistry-immunofluorescence-141120251833319.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85729-img0010.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0011.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0012.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0013.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0014.jpg)
![Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Western-Blot-NB100-59828-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunohistochemistry-NB100-59828-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human CDC20 on HeLa whole cell lysate using NB100-59828. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody NB100-59827.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Western-Blot-NB100-59828-img0006.jpg)
![Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunohistochemistry-NB100-59828-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Cdc20 Antibody [NB100-59828] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59828 (lot NB100-59828-2) used for IP at 6 ug per reaction. CDC20 was also immunoprecipitated by a previous lot of this antibody (lot NB100-59828-1) and rabbit anti-CDC20 antibody NB100-59827. For blotting immunoprecipitated CDC20, NB100-59828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunoprecipitation-NB100-59828-img0013.jpg)
![Western Blot: SMC3 Antibody [NB100-207] - Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SMC3 antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC3-Antibody-Western-Blot-NB100-207-img0026.jpg "Western Blot: SMC3 Antibody [NB100-207] - Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SMC3 antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunohistochemistry-Paraffin-NB100-207-img0028.jpg)
![Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC3-Antibody-Immunohistochemistry-Paraffin-NB100-207-img0027.jpg)
![SMC3 Antibody [NB100-207] - Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC3 antibody NB100-207(lot NB100-207-9) used for IP at 3 ug per reaction. SMC3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-207-8). For blotting immunoprecipitated SMC3, NB100-207 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMC3-Antibody-NB100-207-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with antibodies against Astrin and tubulin and CREST antisera and stained with DAPI for DNA. Cropped images are 3x magnified showing lateral kinetochores. Scale bars represent 5 um (insets, 2 um). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0960982213007653), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74638-img0003.jpg "Immunocytochemistry/Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with antibodies against Astrin and tubulin and CREST antisera and stained with DAPI for DNA. Cropped images are 3x magnified showing lateral kinetochores. Scale bars represent 5 um (insets, 2 um). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0960982213007653), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of Immunoprecipitants. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74638 used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74639, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunoprecipitation-NB100-74638-img0002.jpg)
![Immunohistochemistry: Astrin Antibody [NB100-74638] - SKA3 localisation is not abrogated due to the lack of Astrin.(A) Representative deconvolved images show SKA3 enrichment at kinetochores of HeLa cells treated with Control or Astrin siRNA. Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against Astrin & SKA3 & CREST antiserum (to mark centromeres) & stained with DAPI for DNA. Cropped images show Astrin or SKA3 enrichment at kinetochores. (B) Representative images show SKA3 enrichment at kinetochores of HeLa cells treated with Astrin siRNA before transfection with eukaryotic protein expression vectors encoding YFP-Astrin (WT, 4A or ∆70, as indicated). Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against GFP (for detecting YFP-Astrin) & SKA3 & CREST antiserum & co-stained with DAPI for DNA. Cropped images show YFP-Astrin or SKA3 enrichment at kinetochores. Scale as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31808746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74638_rabbit-polyclonal-astrin-antibody-31020241612667.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NB100-373] - SMC2 in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijcb/2017/8607532/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90956] - Staining of human cerebral cortex, liver, placenta and rectum using Fibrinogen beta chainantibody NBP1-90956 (A) shows similar protein distribution across tissues to independent antibody NBP1-90955 (B).](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90956-img0007.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90956] - Staining of human cerebral cortex, liver, placenta and rectum using Fibrinogen beta chainantibody NBP1-90956 (A) shows similar protein distribution across tissues to independent antibody NBP1-90955 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: Fibrinogen beta chain Antibody [NBP1-90956] - Analysis using Fibrinogen beta chain antibody NBP1-90956 (A) shows similar pattern to independent antibody NBP1-90955 (B).](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Western-Blot-NBP1-90956-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrinogen beta chain Antibody [NBP1-90956] - Staining of human cell line Hep G2 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90956-img0006.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90956] - Staining of human rectum shows strong positivity in plasma in blood vessels, as well as positivity in extracellular matrix.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90956-img0011.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90956] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90956-img0002.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90956] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90956-img0009.jpg)
![Immunohistochemistry-Paraffin: Fibrinogen beta chain Antibody [NBP1-90956] - Staining of human placenta shows strong positivity in plasma in blood vessels.](http://images.novusbio.com/fullsize/Fibrinopeptide-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90956-img0010.jpg)
![Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-288-img0018.jpg "Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-NB100-288-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for IP at 3 ug per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody NB100-639. For blotting immunoprecipitated MCM2, NB100-288 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunoprecipitation-NB100-288-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86635-img0008.jpg "Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SMC4 Antibody [NBP1-86635] - Staining of human kidney shows strong cytoplasmic positivity with granular pattern in tubule cells.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunohistochemistry-Paraffin-NBP1-86635-img0002.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86635_rabbit-polyclonal-smc4-antibody-30420251371429.jpg)
![Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Analysis in human testis and pancreas tissues. Corresponding HSPA2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HSPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-86185-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Analysis in human testis and pancreas tissues. Corresponding HSPA2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HSPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-86185-img0019.jpg)
![Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/HSPA2-Antibody-Immunohistochemistry-Paraffin-NBP1-86185-img0020.jpg)
