| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-91662SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74713 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76538-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71556 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87692-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34760-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27202SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78964SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60491SS | |||
![Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.](http://images.novusbio.com/fullsize/APC-Antibody-Western-Blot-NB100-91662-img0004.jpg "Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.")
![Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/APC-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91662-img0006.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0005.jpg)
![Flow Cytometry: APC Antibody [NB100-91662] - An intracellular stain was performed on HeLa cells with APC Antibody NB100-91662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/APC-Antibody-Flow-Cytometry-NB100-91662-img0007.jpg)
![Western Blot: APC Antibody [NB100-91662] - Extracts from COLO205 cells.](http://images.novusbio.com/fullsize/APC-Antibody-Western-Blot-NB100-91662-img0001.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - Paraffin-embedded human colon carcinoma tissue.](http://images.novusbio.com/fullsize/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0003.jpg)
![Western Blot: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] Analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin Beta 4 monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-3F1-Western-Blot-NBP2-00812-img0009.jpg "Western Blot: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] Analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin Beta 4 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin Beta 4.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-3F1-Immunocytochemistry-Immunofluorescence-NBP2-00812-img0008.jpg)
![Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Human tonsil using anti-Tubulin Beta 4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-00812-img0007.jpg)
![Flow Cytometry: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Analysis of Hela cells, using anti-Tubulin Beta 4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-3F1-Flow-Cytometry-NBP2-00812-img0001.jpg)
![Western Blot: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin Beta 4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tubulin Beta 4.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-3F1-Western-Blot-NBP2-00812-img0002.jpg)
![Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Tubulin Beta 4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-00812-img0003.jpg)
![Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Tubulin Beta 4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-00812-img0004.jpg)
![Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Tubulin Beta 4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-00812-img0005.jpg)
![Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Tubulin Beta 4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-00812-img0006.jpg)
![Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-western-blot-119202416527..gif "<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody [NBP1-76538] - Analysis in rat stomach tissue lysate with antibody at 0.5 ug/mL.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Western-Blot-NBP1-76538-img0004.jpg)
![Immunohistochemistry: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-NBP1-76538-img0003.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody [NBP1-76538] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in 500 uL PBS/BSA/EDTA and viable DAPI cells were analysed using a Galios flow cytometer. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 700 version of this antibody.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Flow-Cytometry-NBP1-76538-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Immunofluorescence of RHAMM/CD168 in human stomach tissue with RHAM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-2711202420243358.jpg)
![Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Gephyrin monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Western-Blot-NBP2-03449-img0018.jpg "Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Gephyrin monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Gephyrin.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NBP2-03449-img0012.jpg)
![Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human prostate tissue using anti-Gephyrin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-03449-img0016.jpg)
![Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Gephyrin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Flow-Cytometry-NBP2-03449-img0011.jpg)
![Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Gephyrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Gephyrin.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Western-Blot-NBP2-03449-img0017.jpg)
![Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Gephyrin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-03449-img0013.jpg)
![Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Gephyrin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-03449-img0014.jpg)
![Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human liver tissue using anti-Gephyrin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-03449-img0015.jpg)
![Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of Jurkat cells, using anti-Gephyrin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Gephyrin-GPHN-Antibody-3B6-Flow-Cytometry-NBP2-03449-img0010.jpg)
![Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546] - Mouse cardiac tissue stained with Troponin I Type 3 antibody. IHC-P image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-Antibody-19C7cc-Immunohistochemistry-Paraffin-NB110-2546-img0003.jpg "Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546] - Mouse cardiac tissue stained with Troponin I Type 3 antibody. IHC-P image submitted by a verifed customer review.")
![Flow Cytometry: Troponin I Type 3 (cardiac) Antibody (19C7) [NB110-2546] - FACS analysis of Troponin I Type 3 in differentiated ESC cultures using anti-Troponin I Type 3 (cardiac) antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-Antibody-19C7-Flow-Cytometry-NB110-2546-img0002.jpg)
![Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP1-87692-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP1-87692] - Staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87692-img0010.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] - Staining of human skeletal muscle shows moderate nuclear membrane positivity in myocytes.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunohistochemistry-Paraffin-NBP1-87692-img0006.jpg)
![Western Blot: Emerin Antibody [NBP1-87692] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP1-87692-img0011.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] -Staining of human heart muscle shows strong positivity in nuclear membrane in cardiomyocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-87692_rabbit-polyclonal-emerin-antibody-immunohistochemistry-paraffin-21122023105440..jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] -Staining of human fallopian tube shows moderate positivity in nuclear membrane in glandular cells and smooth muscle cells with additional weak cytoplasmic positivity.](http://images.novusbio.com/fullsize/antibody/nbp1-87692_rabbit-polyclonal-emerin-antibody-immunohistochemistry-paraffin-21122023105539..jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692]- Staining of human testis shows moderate positivity in nuclear membrane in cells in seminiferous ducts and leydig cells with weak cytoplasmic positivity.](http://images.novusbio.com/fullsize/antibody/nbp1-87692_rabbit-polyclonal-emerin-antibody-immunohistochemistry-paraffin-21122023105658..jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunocytochemistry-Immunofluorescence-NBP2-32808-img0009.jpg "Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with alpha-Smooth Muscle Actin Antibody (SPM332) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).")
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Staining of paraffin-embedded Leiomyosarcoma using the Azide and BSA-Free version of alpha-Smooth Muscle Actin (SPM332) Antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Immunohistochemistry-Paraffin-NBP2-32808-img0004.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0008.jpg)
![Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP. Image from the PerCP version of this antibody.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-SPM332-Flow-Intracellular-NBP2-32808-img0006.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0015.jpg "Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Rat kidney. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - C2C12 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0003.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - HBL435 xenograft, using RAD21 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Paraffin-embedded human lung papillary adenocarcinoma. RAD21 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0006.jpg)
![Immunohistochemistry-Paraffin: Rad21 Antibody [NBP2-20053] - Mouse testis. Rad21 stained by Rad21 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunohistochemistry-Paraffin-NBP2-20053-img0014.jpg)
![Immunoprecipitation: Rad21 Antibody [NBP2-20053] - Jurkat whole cell extracts using 5 ug of RAD21 antibody. Western blot analysis was performed using RAD21 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Rad21-Antibody-Immunoprecipitation-NBP2-20053-img0010.jpg)
![Western Blot: Rad21 Antibody [NBP2-20053] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (NBP2-20053) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20053_rabbit-polyclonal-rad21-antibody-91020242063390.jpg)
![Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NBP2-27202-img0001.jpg "Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-14-3-3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27202-img0004.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27202-img0002.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27202-img0003.jpg)
![Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP1-78964-img0015.jpg "Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.")
![Immunocytochemistry/Immunofluorescence: LC3A Antibody [NBP1-78964] - IF Confocal analysis of A549 cells using LC3I antibody (NBP1-78964, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78964-img0017.jpg)
![Immunohistochemistry: LC3A Antibody [NBP1-78964] - Analysis of LC3I in mouse testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-NBP1-78964-img0016.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Simple Western: Dynamin Antibody (3G4B6) [NB110-60491] - Simple Western lane view shows a specific band for Dynamin in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Simple-Western-NB110-60491-img0012.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0003.jpg)
![Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491] - Antibody-bead conjugates were prepared by adding 1.0 ug of antibody to 500 ul of PBS with 0,01% triton X-100 in a microcentrifuge tube and 30ul of protein G Sepharose beads. Tubes were rocked O/N at 4 degrees C followed by several washes to remove unbound antibodies. U2OS were collected in HEPES buffer (20 mM HEPES, 100 mM sodium chloride, 1 mM EDTA, 1% Triton X-100, pH 7.4) supplemented with protease inhibitor. Lysates are rocked 30 min at 4 degrees C and spun at 110,000xg for 15 min at 4 degrees C. One ml aliquots at 1 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4 degrees C. Following centrifugation, the unbound fractions were collected, and beads were subsequently washed three times with 1 ml of HEPES lysis buffer and processed for SDS-PAGE and immunoblot. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunoprecipitation-NB110-60491-img0014.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0002.jpg)
![Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [NB110-60491] - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using Dynamin antibody with DAB staining.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunohistochemistry-Paraffin-NB110-60491-img0004.jpg)
![Flow Cytometry: Dynamin Antibody (3G4B6) [NB110-60491] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Flow-Cytometry-NB110-60491-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Dynamin Antibody (3G4B6) [NB110-60491] - U2OS WT and DNM1 KO cells were labelled with a green or a far red fluorescence dye, respectively. WT and KO cells were mixed and plated to a 1 :1 ratio on coverslips. Cells were stained with and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining) and far-red (KO) channels was performed. Representative grayscale image of the red channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0015.jpg)
![Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-8F4-Immunohistochemistry-Frozen-NBP1-51148-img0001.jpg "Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.")