Cell Biology reagents: Novus Biologicals

NBP1-85799

Rab13 Antibody - BSA Free

(5 Publications)

0.1 ml

NBP1-85799

Immunocytochemistry/Immunofluorescence: Rab13 Antibody [NBP1-85799] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human stomach shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human prostate shows weak membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Western Blot: Rab13 Antibody [NBP1-85799] - RABIF distribution & effect on RAB13 expressionRepresentative immunofluorescence images of RABIF protein in cells transfected with the indicated siRNAs. Reduction of intensity in knockdown cells confirms the specificity of the signal. Calibration bar shows intensity values. Note that RABIF exhibits a mostly perinuclear enrichment.RAB13 expression in cells with CRISPR knockdown of RABIF using the indicated sgRNAs (see also Fig 7C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 RNA mislocalization does not affect RAB13 binding to membranes or association with REP‐1 or RabGDICells treated with the indicated PMOs were fractionated into soluble & particulate fractions, & the indicated proteins were detected by Western blot. RhoGDI & TfRc serve as soluble & particulate markers, respectively.Lysates from the indicated GFP or GFP‐RAB13‐expressing cell lines were immunoprecipitated with anti‐GFP antibodies & blotted to detect the indicated proteins. Relative REP‐1 & RabGDI binding are quantified in the graphs from n = 3 (REP‐1) & n = 5 (RabGDI) independent experiments. Bars: mean ± s.e.m. No significant differences were detected by Wilcoxon matched‐pairs signed‐rank test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 RNA & protein exhibit distinct subcellular distributionsRepresentative FISH images showing RAB13 RNA distribution in MDA‐MB-231 cells. Nuclei & cell outlines are shown in blue & green, respectively. Arrows point to RAB13 RNA concentrated at protrusive regions. Boxed regions are magnified in the insets.Representative immunofluorescence images of RAB13 protein in cells transfected with the indicated siRNAs. Reduction of intensity in RAB13 knockdown cells confirms the specificity of the signal. Arrows point to perinuclear RAB13 protein. Calibration bar shows intensity values.Ratios of peripheral/perinuclear intensity calculated from images as shown in (A) & (B). Bars: mean ± s.e.m. Values within each bar represent number of cells observed in 3 independent experiments.Protrusions (Ps) & cell bodies (CB) of cells induced to migrate toward LPA were isolated & analyzed to detect the indicated proteins (by Western blot; left panels) or RNAs (by RT‐ddPCR; right panel). Ps/CB enrichment ratios from 2 independent experiments are shown. Bars: mean ± s.e.m. The enrichment of pY397‐FAK serves to verify the enrichment of protrusions containing newly formed adhesions in the Ps fraction.Data information: P‐values: **< 0.01; ****< 0.0001 by Student's t‐test (C) or analysis of variance with Dunnett's multiple comparisons test against GFP (D). Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: Rab13 Antibody [NBP1-85799] - Peripheral RAB13 RNA translation is required for RAB13 protein activation but not steady‐state distribution or membrane associationAWide‐field images of RAB13 immunofluorescence in MDA‐MB-231 cells treated with control or RAB13 (191 + 230) PMOs & ratios of peripheral/perinuclear intensity. Scale bars: 10 μm. n = 45–50 cells. Bars: mean ± s.e.m. Similar results were observed in two additional independent experiments.BFluorescence images (projections of confocal slices throughout the cell height) of cells expressing GFP‐RAB13 with the indicated UTRs. Note that in both cases the protein assumes indistinguishable distribution. Scale bars: 10 μm.CSoluble/particulate fractionation of the indicated cell lines followed by Western blot to detect the indicated proteins. RhoGDI & TfRc serve as soluble & particulate markers, respectively. Graph shows quantitation from n = 3 independent experiments. Bars: mean ± s.e.m.D, EActive RAB13 (RAB13‐GTP) was pulled down using MICAL‐L1 RBD‐GST from the indicated PMO‐treated cells (D) or GFP‐RAB13‐expressing lines (E). The amount of endogenous or exogenous RAB13 was measured by quantitative Western blot, & relative levels of active RAB13 are plotted. n = 8 (D), n = 4 (E). Bars: mean ± s.e.m.Data information: P‐values: *< 0.05, **< 0.01 by Kruskal–Wallis test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Immunocytochemistry/ Immunofluorescence: Rab13 Antibody [NBP1-85799] - RAB13 protein levels do not change upon serum stimulationMDA‐MB‐231 cells were stimulated with serum for the indicated times, in the presence or absence of cycloheximide (CHX). The cells were also treated with control or RAB13‐mislocalizing PMOs.Representative Western blot analysis of whole‐cell lysates & corresponding quantitations of RAB13 levels from n = 4–5 replicates. Bars: mean ± s.e.m. No significant differences by Friedman's test. Increase in pY397‐FAK levels attests to serum stimulation.RAB13 immunofluorescence at representative protrusive regions. A cell line expressing GFP was used to delineate cell borders & provide an internal cytosolic control. RAB13 signal at front lamellipodial regions was quantified. n = 35–51 protrusions. Bars: mean ± s.e.m. No increase is detected upon stimulation. By contrast, at early time points a decrease is detected (5 & 20 min, P < 0.01 by Kruskal–Wallis test), potentially arising from serum‐induced endocytosis of RAB13‐containing membranes. Scale bars: 8 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 protein levels do not change upon serum stimulationMDA‐MB‐231 cells were stimulated with serum for the indicated times, in the presence or absence of cycloheximide (CHX). The cells were also treated with control or RAB13‐mislocalizing PMOs.Representative Western blot analysis of whole‐cell lysates & corresponding quantitations of RAB13 levels from n = 4–5 replicates. Bars: mean ± s.e.m. No significant differences by Friedman's test. Increase in pY397‐FAK levels attests to serum stimulation.RAB13 immunofluorescence at representative protrusive regions. A cell line expressing GFP was used to delineate cell borders & provide an internal cytosolic control. RAB13 signal at front lamellipodial regions was quantified. n = 35–51 protrusions. Bars: mean ± s.e.m. No increase is detected upon stimulation. By contrast, at early time points a decrease is detected (5 & 20 min, P < 0.01 by Kruskal–Wallis test), potentially arising from serum‐induced endocytosis of RAB13‐containing membranes. Scale bars: 8 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

<b>Genetic Strategies Validation. </b>Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB13 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85799-25ul

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NB100-65101

CD68/SR-D1 Antibody (514H12) - BSA Free

(15 Publications)

0.1 ml

NB100-65101

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-65101-0.025ml

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NB120-5579

HSP27 Antibody

(1 Review)

(2 Publications)

100μg

NB120-5579

Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.

Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.

Reactivity:

Hu, Rt, Pm, Ca

Species Glossary

Reactivity: Hu, Rt, Pm, Ca

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-56641

Troponin I Type 1 (slow skeletal) Antibody - BSA Free

(9 Publications)

100 ul

NBP1-56641

$Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Western-blots for a selection of differentially-expressed proteins. MyH2b, Troponin I Type 1 (slow skeletal) (TNNI1) and MCK were used as target protein; GADPH was used as a control. Total protein from control and arginine-supplemented pig samples was fractionated by SDS-PAGE, blotted and then detected with specific antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117294), licensed under a CC-BY license.$

Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.

$Western-blots for a selection of differentially-expressed proteins.MyH2b, TNNI1 and MCK were used as target protein; GADPH was used as a control. A: Total protein from control and arginine-supplemented pig samples was fractionated by SDS-PAGE, blotted and then detected with specific antibodies; B: Semi-quantification of protein content computed using Image J software. Data are presented as the mean ±SEM (n = 6).$

Reactivity:

Hu, Mu, Po

Species Glossary

Reactivity: Hu, Mu, Po

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-37577

AIF Antibody (4E7)

(4 Publications)

0.1 ml

NBP2-37577

Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human lung cancer tissues using AIF mouse mAb with DAB staining.

Flow Cytometry: AIF Antibody (4E7) [NBP2-37577] - Analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).

Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)

Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human breast cancer tissues using AIF mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human cervical cancer tissues using AIF mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human rectum cancer tissues using AIF mouse mAb with DAB staining.

ELISA: AIF Antibody (4E7) [NBP2-37577] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu, Mu, Rt, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-75707

Villin 1 Antibody (JU34-75)

(1 Review)

100 ul

NBP2-75707

Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded mouse colon tissue using anti-Villin1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of HeLa cells with Villin1 antibody at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Villin 1 Antibody (JU34-75) [NBP2-75707] - Caco-2 cell line lysate. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (JU34-75) [NBP2-75707] - Staining Villin1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded human colon cancer tissue using anti-Villin1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Villin 1 Antibody (JU34-75) [NBP2-75707] - Analysis of paraffin-embedded human kidney tissue using anti-Villin1 antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NB500-318

ICAM-1/CD54 Antibody (MEM-111) - BSA Free

(7 Publications)

0.1 mg

NB500-318

Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Of CD54 expression in activated (A) and nonactivated (B) Huvec cells by antibody MEM-111. Lower bands represent tubulin as a loading control.

Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using the FITC conjugate of NB500-318. Staining of Human peripheral blood lymphocytes with MEM-111 FITC.

Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using PE conjugate of NB500-318. A cell surface stain was performed on THP-1 cells with ICAM-1/CD54 antibody (MEM-111) NB500-318 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.

Reactivity:

Hu, Rt, Bv

Species Glossary

Reactivity: Hu, Rt, Bv

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-318AF350
Alexa Fluor 405	NB500-318AF405
Alexa Fluor 488	NB500-318AF488
Alexa Fluor 532	NB500-318AF532
Alexa Fluor 594	NB500-318AF594
Alexa Fluor 647	NB500-318AF647
Alexa Fluor 700	NB500-318AF700
Alexa Fluor 750	NB500-318AF750
Allophycocyanin	NB500-318APC
Allophycocyanin/Cy7	NB500-318APCCY7
Biotin	NB500-318B
CoraFluor 1	NB500-318CL1
DyLight 350	NB500-318UV
DyLight 405	NB500-318V
DyLight 488	NB500-318G
DyLight 550	NB500-318R
DyLight 594	NB500-318DL594
DyLight 650	NB500-318C
DyLight 680	NB500-318FR
DyLight 755	NB500-318IR
FITC	NB500-318F
HRP	NB500-318H
Janelia Fluor 525	NB500-318JF525
Janelia Fluor 549	NB500-318JF549
Janelia Fluor 646	NB500-318JF646
Janelia Fluor 669	NB500-318JF669
PE	NB500-318PE
PE/Atto594	NB500-318PEATT594
PE/Cy5.5	NB500-318PECY55
PE/Cy7	NB500-318PECY7
PerCP	NB500-318PCP
mFluor Violet 450 SE	NB500-318MFV450
mFluor Violet 500 SE	NB500-318MFV500
mFluor Violet 610 SE	NB500-318MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB500-318-25ug

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NBP2-45225

S100B Antibody (15F4NB) - Azide and BSA Free

(1 Publication)

0.1 mg

NBP2-45225

Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate

Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).

Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.

Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.

Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.

Reactivity:

Hu, Mu, Rt, Po, Bv, Eq, Pm, Rb, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Eq, Pm, Rb, Sh

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-24389

ATG5 Antibody - BSA Free

(7 Publications)

0.1 mg

NBP2-24389

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6085A

Alt Catalog# IMG-6085A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24389AF350
Alexa Fluor 405	NBP2-24389AF405
Alexa Fluor 488	NBP2-24389AF488
Alexa Fluor 532	NBP2-24389AF532
Alexa Fluor 594	NBP2-24389AF594
Alexa Fluor 647	NBP2-24389AF647
Alexa Fluor 700	NBP2-24389AF700
Alexa Fluor 750	NBP2-24389AF750
Allophycocyanin	NBP2-24389APC
Biotin	NBP2-24389B
CoraFluor 1	NBP2-24389CL1
DyLight 350	NBP2-24389UV
DyLight 405	NBP2-24389V
DyLight 488	NBP2-24389G
DyLight 550	NBP2-24389R
DyLight 594	NBP2-24389DL594
DyLight 650	NBP2-24389C
DyLight 680	NBP2-24389FR
DyLight 755	NBP2-24389IR
FITC	NBP2-24389F
HRP	NBP2-24389H
Janelia Fluor 525	NBP2-24389JF525
Janelia Fluor 549	NBP2-24389JF549
Janelia Fluor 646	NBP2-24389JF646
Janelia Fluor 669	NBP2-24389JF669
PE	NBP2-24389PE
PerCP	NBP2-24389PCP
mFluor Violet 450 SE	NBP2-24389MFV450
mFluor Violet 500 SE	NBP2-24389MFV500
mFluor Violet 610 SE	NBP2-24389MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24389SS

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NB100-56518

TRAIL/TNFSF10 Antibody (55B709.3) - BSA Free

(9 Publications)

0.1 mg

NB100-56518

Reactivity:

Hu, Ca, Fe

Species Glossary

Reactivity: Hu, Ca, Fe

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-256A

Alt Catalog# IMG-256A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56518AF350
Alexa Fluor 405	NB100-56518AF405
Alexa Fluor 488	NB100-56518AF488
Alexa Fluor 532	NB100-56518AF532
Alexa Fluor 594	NB100-56518AF594
Alexa Fluor 647	NB100-56518AF647
Alexa Fluor 700	NB100-56518AF700
Alexa Fluor 750	NB100-56518AF750
Allophycocyanin	NB100-56518APC
Biotin	NB100-56518B
CoraFluor 1	NB100-56518CL1
DyLight 350	NB100-56518UV
DyLight 405	NB100-56518V
DyLight 488	NB100-56518G
DyLight 550	NB100-56518R
DyLight 594	NB100-56518DL594
DyLight 650	NB100-56518C
DyLight 680	NB100-56518FR
DyLight 755	NB100-56518IR
FITC	NB100-56518F
HRP	NB100-56518H
Janelia Fluor 525	NB100-56518JF525
Janelia Fluor 549	NB100-56518JF549
Janelia Fluor 646	NB100-56518JF646
Janelia Fluor 669	NB100-56518JF669
PE	NB100-56518PE
PerCP	NB100-56518PCP
mFluor Violet 450 SE	NB100-56518MFV450
mFluor Violet 500 SE	NB100-56518MFV500
mFluor Violet 610 SE	NB100-56518MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56518SS
Azide and BSA Free	NBP2-80989

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NBP2-32945

TfR (Transferrin R) Antibody (DF1513)

(1 Review)

(2 Publications)

0.1 mg

NBP2-32945

Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).

Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 lysates(s) respectively.

The effect of NO on iron handling gene expression in human placental villous explants. (A–E) qPCR results, (F–J) protein analyses. Representative blots are shown in (K). HO-1 and TfR mRNA levels are significantly increased in the group treated with NO compared to the untreated controls. Protein abundance of HO-1 is significantly increased in the group treated with NO compared to the untreated controls. No significant change in expression is seen in the rest of the genes studied. HO-1: heme oxygenase 1, HAMP: hepcidin antimicrobial peptide, TfR: transferrin receptor, DMT1: divalent metal transporter 1, FPN: ferroportin 1. Data are reported from at least three independent experiments as mean +/- SD, *** p < 0.001, ns = not significant. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/6/5887), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The effect of hypoxia on iron handling genes in response to NO in syncitialized BeWo cells (SCTs). (A–F) qPCR results and (G–K) protein analyses. Representative blots are shown in (K). Hypoxia alone did not significantly alter mRNA or protein levels of any of the studied iron homeostasis markers compared to normoxic controls. (A,B) The combination of hypoxia and NO resulted in a significant increase in HO-1 and HAMP mRNA levels, while NO increased HAMP mRNA levels significantly under hypoxic conditions. No significant effect of hypoxia or NO was seen in any of the other targets studied. HO-1: Heme Oxygenase 1, HAMP: Hepcidin antimicrobial peptide, TfR: Transferrin Receptor, TF: Transferrin, DMT1, Divalent metal transporter 1, FPN: Ferroportin 1. Data are reported from at least three independent experiments as mean +/- SD, * p < 0.05, ns = not significant. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/6/5887), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The effect of NO on iron handling genes in syncytialized BeWo cells. (A–E), qPCR results, and (E–J) protein analyses. Representative blots are shown in (K). HO-1 and HAMP mRNA levels are significantly increased in the group treated with NO compared to the untreated controls. Protein abundance of HO-1 is significantly increased in the group treated with NO compared to the untreated controls. No significant change in expression is seen in the rest of the genes studied. HO-1: heme oxygenase 1, HAMP: hepcidin antimicrobial peptide, TfR: transferrin receptor, DMT1: divalent metal transporter 1, FPN: ferroportin 1. Data are reported from at least three independent experiments as mean +/- SD, ** p < 0.01, **** p < 0.0001, ns = not significant. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/6/5887), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-34602AF350
Alexa Fluor 405	NBP2-34602AF405
Alexa Fluor 488	NBP2-34602AF488
Alexa Fluor 532	NBP2-34602AF532
Alexa Fluor 594	NBP2-34602AF594
Alexa Fluor 647	NBP2-34602AF647
Alexa Fluor 700	NBP2-34602AF700
Alexa Fluor 750	NBP2-34602AF750
Allophycocyanin	NBP2-34602APC
Allophycocyanin/Cy7	NBP2-34602APCCY7
Biotin	NBP2-34602B
CoraFluor 1	NBP2-34602CL1
DyLight 350	NBP2-34602UV
DyLight 405	NBP2-34602V
DyLight 488	NBP2-34602G
DyLight 550	NBP2-34602R
DyLight 594	NBP2-34602DL594
DyLight 650	NBP2-34602C
DyLight 680	NBP2-34602FR
DyLight 755	NBP2-34602IR
FITC	NBP2-34602F
HRP	NBP2-34602H
Janelia Fluor 525	NBP2-34602JF525
Janelia Fluor 549	NBP2-34602JF549
Janelia Fluor 646	NBP2-34602JF646
Janelia Fluor 669	NBP2-34602JF669
PE	NBP2-34602PE
PE/Atto594	NBP2-34602PEATT594
PE/Cy5.5	NBP2-34602PECY55
PE/Cy7	NBP2-34602PECY7
PerCP	NBP2-34602PCP
mFluor Violet 450 SE	NBP2-34602MFV450
mFluor Violet 500 SE	NBP2-34602MFV500
mFluor Violet 610 SE	NBP2-34602MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-34602-0.1mg

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NBP1-88706

SON Antibody - BSA Free

(1 Review)

(5 Publications)

0.1 ml

NBP1-88706

Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human cerebellum shows moderate to strong positivity in nuclear speckles in Purkinje cells.

Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human small intestine shows moderate to strong positivity in nuclear speckles in glandular cells.

Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human tonsil shows moderate to strong positivity in nuclear speckles in germinal center cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-88706-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Rab13 Antibody - BSA Free

CD68/SR-D1 Antibody (514H12) - BSA Free

HSP27 Antibody

Troponin I Type 1 (slow skeletal) Antibody - BSA Free

AIF Antibody (4E7)

Villin 1 Antibody (JU34-75)

ICAM-1/CD54 Antibody (MEM-111) - BSA Free

S100B Antibody (15F4NB) - Azide and BSA Free

ATG5 Antibody - BSA Free

TRAIL/TNFSF10 Antibody (55B709.3) - BSA Free

TfR (Transferrin R) Antibody (DF1513)

SON Antibody - BSA Free