Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free

Western Blot: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Gephyrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Gephyrin.

Immunocytochemistry/Immunofluorescence: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Gephyrin.

Immunohistochemistry: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - Staining of paraffin-embedded Human liver tissue using anti-Gephyrin mouse monoclonal antibody.

Flow Cytometry: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Gephyrin antibody, and then analyzed by flow cytometry.

Western Blot: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Gephyrin monoclonal antibody.

Immunohistochemistry: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Gephyrin mouse monoclonal antibody.

Immunohistochemistry: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Gephyrin mouse monoclonal antibody.

Immunohistochemistry: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - Staining of paraffin-embedded Human prostate tissue using anti-Gephyrin mouse monoclonal antibody.

Flow Cytometry: Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free [NBP2-71556] - Analysis of Jurkat cells, using anti-Gephyrin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human GPHN (NP_065857) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPHN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 83.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Gephyrin/GPHN Antibody (OTI3B6) - Azide and BSA Free

• GEPH
• Gephyrin
• GPH
• GPHN
• GPHRYN
• KIAA1385GPHGEPH

Background

The Gephyrin (GPHN) gene encodes a neuronal assembly protein that exists in isoform 1 (736 amino acids long, 79 kDA) or isoform 2 (769 amino acids in length, approximately 83 kDA). This protein anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton through high affinity binding to a receptor subunit domain and tublin dimers. The protein coded by GPHN is also necessary for molybdenum biosynthesis. The GPHN gene has been investigated in relation to many diseases such as leukemia, cervicitis, tuberculosis, alzheimer's disease, anorexia nervosa, huntington's disease, retinitis, and herpes zoster. The GPHN gene interacts with genes DYNLL1, PFN1, PRPF4, ENAH, and SLX4 to participate in pathways that regulate metabolism of vitamins and cofactors as well as molybdenum cofactor biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPHN