Product Search for integrin alpha: Novus Biologicals

AF3827

Integrin alpha 9 Antibody [Unconjugated]

100 μg

AF3827

CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Integrin a9 was detected in perfusion fixed frozen sections of mouse liver tissue using Goat Anti-Mouse Integrin a9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3827) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Integrin a9 was detected in perfusion fixed frozen sections of mouse liver tissue using Goat Anti-Mouse Integrin a9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3827) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Morphology and protein expression of hSKPs. (a) SEM micrographs of nanofibrous scaffolds showing morphology of hSKPs at 7 days of culture. (b) Immunofluorescent images of hSKPs grown on nanofibrous scaffolds showing expression of integrin alpha -9 (green), fibronectin (red) and procollagen I & III (green). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28860484), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Intergins alpha 4/ alpha 9 are responsible for the action of osteopontin (OPN) in the augmentation of macrophage (Mφ) M2 polarization and angiogenic capacity. (A) Relative mean fluorescence intensities (MFIs) of integrins alpha 4/ alpha 9 were measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 antibodies (Abs) and each isotype-matched control Ab were used at 10 µg/mL, n = 4 [***p < 0.001 vs. untreated, ###p < 0.001 vs. interleukin (IL)-10 alone]. (B) Representative confocal laser scanning immunofluorescence overlay images of integrin alpha 4 (green) and DAPI (blue), as well as those of integrin alpha 9 (red) and DAPI (blue) in Mφ (–) and Mφ (IL-10 + IL-18). Scale bar represents 20 µm. (C) Relative MFI of CD163 was measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 4 (***p < 0.001 vs. untreated, ###p < 0.001 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (D) The total areas and lengths of tube-like structures were determined by the Matrigel tube formation assay where b.End5 was cocultured with each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 6 (***p < 0.001, **p < 0.01, *p < 0.05 vs. untreated, ###p < 0.001, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). Integrin alpha 4 = ITGA4; Integrin alpha 9 = ITGA9. All data are expressed as means ± SEM and were analyzed by a one-way ANOVA followed by Tukey’s test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29559970), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3827U-100UG
Alexa Fluor 405	AF3827V-100UG
Alexa Fluor 488	AF3827G-100UG
Alexa Fluor 532	AF3827X-100UG
Alexa Fluor 594	AF3827T-100UG
Alexa Fluor 647	AF3827R-100UG
Alexa Fluor 700	AF3827N-100UG
Alexa Fluor 750	AF3827S-100UG
Alexa Fluor Plus 405	AF3827AFP405-100UG
Alexa Fluor Plus 488	AF3827AFP488-100UG
Alexa Fluor Plus 555	AF3827AFP555-100UG
Alexa Fluor Plus 594	AF3827AFP594-100UG
Alexa Fluor Plus 647	AF3827AFP647-100UG
Alexa Fluor Plus 680	AF3827AFP680-100UG
Allophycocyanin	AF3827A
Biotin	AF3827B
CoraFluor 1	AF3827CL1
DyLight 350	AF3827D
DyLight 405	AF3827E
DyLight 488	AF3827K
DyLight 550	AF3827L
DyLight 594	AF3827M
DyLight 650	AF3827W
DyLight 680	AF3827Y
DyLight 755	AF3827Z
FITC	AF3827F
HRP	AF3827H
Janelia Fluor 525	AF3827JF525
Janelia Fluor 549	AF3827I
Janelia Fluor 646	AF3827J
Janelia Fluor 669	AF3827JF669
PerCP	AF3827C
Phycoerythrin	FAB3827P
mFluor Violet 450 SE	AF3827MFV450
mFluor Violet 500 SE	AF3827MFV500
mFluor Violet 610 SE	AF3827MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3827P
Carrier Free	AF3827-SP

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AF4076

Integrin alpha 8 Antibody [Unconjugated]

(26 Publications)

100 μg

AF4076

HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4076U-100UG
Alexa Fluor 405	AF4076V-100UG
Alexa Fluor 488	AF4076G-100UG
Alexa Fluor 532	AF4076X-100UG
Alexa Fluor 594	AF4076T-100UG
Alexa Fluor 647	AF4076R-100UG
Alexa Fluor 700	AF4076N-100UG
Alexa Fluor 750	AF4076S-100UG
Alexa Fluor Plus 405	AF4076AFP405-100UG
Alexa Fluor Plus 488	AF4076AFP488-100UG
Alexa Fluor Plus 555	AF4076AFP555-100UG
Alexa Fluor Plus 594	AF4076AFP594-100UG
Alexa Fluor Plus 647	AF4076AFP647-100UG
Alexa Fluor Plus 680	AF4076AFP680-100UG
Allophycocyanin	AF4076A
Biotin	BAF4076
CoraFluor 1	AF4076CL1
DyLight 350	AF4076D
DyLight 405	AF4076E
DyLight 488	AF4076K
DyLight 550	AF4076L
DyLight 594	AF4076M
DyLight 650	AF4076W
DyLight 680	AF4076Y
DyLight 755	AF4076Z
FITC	AF4076F
HRP	AF4076H
Janelia Fluor 525	AF4076JF525
Janelia Fluor 549	AF4076I
Janelia Fluor 646	AF4076J
Janelia Fluor 669	AF4076JF669
PE	AF4076P
PerCP	AF4076C
mFluor Violet 450 SE	AF4076MFV450
mFluor Violet 500 SE	AF4076MFV500
mFluor Violet 610 SE	AF4076MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4076-SP

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NBP1-86118

Integrin alpha 7 Antibody - BSA Free

(1 Review)

(1 Publication)

0.1 ml

NBP1-86118

Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-86118-25ul

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NBP2-16972

Integrin alpha 9 Antibody

(1 Review)

(3 Publications)

0.1 ml

NBP2-16972

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 9 antibody [N1N2], N-term (NBP2-16972) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Bv

Species Glossary

Reactivity: Hu, Mu, Bv

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-86519

Integrin alpha 8 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-86519

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP1-86519] - Staining of human lung shows weak to moderate membranous positivity in pneumocytes.

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP1-86519] - Staining of human small intestine shows weak to moderate membranous positivity in smooth muscle cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-86519-25ul

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MAB4574

Integrin alpha 9 Antibody (560201)

(6 Publications)

100 μg

MAB4574

HAF007). A specific band was detected for Integrin a9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Integrin a9 Monoclonal Antibody (Catalog # MAB4574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Integrin a9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Integrin a9 Monoclonal Antibody (Catalog # MAB4574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Expression of VEGF-D-binding receptors by 468LN cells:(A) Expression of VEGF-R2 and R3 mRNA examined with semi quantitative RT-PCR, and (B) alpha 9 integrin mRNA measured with qRT-PCR. (C) Confirmation of VEGF-R2, VEGF-R3, and alpha 9 integrin expression at protein levels by western blot. MDA-MB-231 and MCF7-COX-2 cells were used as positive controls primarily for VEGF-R2 and VEGF-R3 expression. Differentially high alpha 9 integrin expression by 468 LN cells was evident. Bars represent mean (n = 4) ± SE, *,P<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0035094), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4574U-100UG
Alexa Fluor 405	FAB4574V-100UG
Alexa Fluor 488	FAB4574G-100UG
Alexa Fluor 532	FAB4574X-100UG
Alexa Fluor 594	FAB4574T-100UG
Alexa Fluor 647	FAB4574R-100UG
Alexa Fluor 700	FAB4574N-100UG
Alexa Fluor 750	FAB4574S-100UG
Alexa Fluor Plus 405	FAB4574AFP405-100UG
Alexa Fluor Plus 488	FAB4574AFP488-100UG
Alexa Fluor Plus 555	FAB4574AFP555-100UG
Alexa Fluor Plus 594	FAB4574AFP594-100UG
Alexa Fluor Plus 647	FAB4574AFP647-100UG
Alexa Fluor Plus 680	FAB4574AFP680-100UG
Allophycocyanin	FAB4574A
Biotin	FAB4574B
CoraFluor 1	FAB4574CL1
DyLight 350	FAB4574D
DyLight 405	FAB4574E
DyLight 488	FAB4574K
DyLight 550	FAB4574L
DyLight 594	FAB4574M
DyLight 650	FAB4574W
DyLight 680	FAB4574Y
DyLight 755	FAB4574Z
FITC	FAB4574F
HRP	FAB4574H
Janelia Fluor 525	FAB4574JF525
Janelia Fluor 549	FAB4574I
Janelia Fluor 646	FAB4574J
Janelia Fluor 669	FAB4574JF669
PE	FAB4574P
PerCP	FAB4574C
mFluor Violet 450 SE	FAB4574MFV450
mFluor Violet 500 SE	FAB4574MFV500
mFluor Violet 610 SE	FAB4574MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4574-SP

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MAB4235

Integrin alpha 11 Antibody (396214) [Unconjugated]

(5 Publications)

100 μg

MAB4235

HAF005). A specific band was detected for Integrin a11 at approximately 150 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human heart tissue. PVDF membrane was probed with 2 µg/mL of Human/Mouse Integrin a11 Monoclonal Antibody (Catalog # MAB4235) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for Integrin a11 at approximately 150 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human heart tissue. PVDF membrane was probed with 2 µg/mL of Human/Mouse Integrin a11 Monoclonal Antibody (Catalog # MAB4235) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Integrin a11 at approximately 150 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Genetic Strategies Validation. </b>YAP-1 and MYL9 mediate pro-fibrotic aspects of liver fibrosis in activated HSCs.(a,b) Quantified increase in total MYL9 and YAP-1 protein levels on activation of rat HSCs from n≥3 experiments (a) with representative immunoblot shown in b. (c–e) Total YAP-1 and MYL9 are diminished in activated mouse HSCs (‘Control') following integrin beta-1 loss (‘Itgb1-null'). Quantification from n=3 experiments shown in c with representative immunoblot in d. In the immunofluorescence in e, note the rounded inactivated appearance of the Itgb1-null cells. The remaining total YAP-1 signal is more cytoplasmic (see h,i). Scale bar, 50 μm. (f,g) Itga11 knockdown in activated rat HSCs using two different siRNA oligos. Data for each oligo are shown relative to its own scrambled control in either black or grey (n=3 for each). (f) Detection of Myl9 transcripts was diminished to an almost identical extent as for Itga11. (g) Protein detection of MYL9 and total YAP-1 was also diminished following Itga11 knockdown. (h,i) The proportion of phosphorylated YAP (PYAP, inactive form), is increased following integrin beta-1 loss (‘Itgb1-null') from activated mouse HSCs (‘Control'; n=3 experiments) and localises more predominantly to the cytoplasm (i). DAPI, blue nuclear counterstain, is shown. Scale bar, 50 μm. (j) Luciferase activity (in relative light units; RLU) following co-transfection of constructs containing the wild-type (MYL9-TEAD) or mutated (MYL9-delta TEAD) TEAD motif from the 3′-untranslated region of the MYL9 gene with empty vector (Control) or YAP expression vector. Results are normalized to a Renilla vector and expressed relative to the control MYL9 luciferase construct without YAP. (k) Transcript levels by qRT–PCR following inhibition of YAP-TEAD interaction using VP in activated rat HSCs expressed relative to DMSO control. Two-tailed unpaired t-test was used for statistical analysis. Data are shown as means±s.e.m. *P<0.05, **P<0.01, †P<0.005, ‡P<0.001. Image collected and cropped by Cite

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4235U-100UG
Alexa Fluor 405	FAB4235V-100UG
Alexa Fluor 488	FAB4235G-100UG
Alexa Fluor 532	FAB4235X-100UG
Alexa Fluor 594	FAB4235T-100UG
Alexa Fluor 647	FAB4235R-100UG
Alexa Fluor 700	FAB4235N-100UG
Alexa Fluor 750	FAB4235S-100UG
Alexa Fluor Plus 405	FAB4235AFP405-100UG
Alexa Fluor Plus 488	FAB4235AFP488-100UG
Alexa Fluor Plus 555	FAB4235AFP555-100UG
Alexa Fluor Plus 594	FAB4235AFP594-100UG
Alexa Fluor Plus 647	FAB4235AFP647-100UG
Alexa Fluor Plus 680	FAB4235AFP680-100UG
Allophycocyanin	FAB4235A
Biotin	FAB4235B
CoraFluor 1	FAB4235CL1
DyLight 350	FAB4235D
DyLight 405	FAB4235E
DyLight 488	FAB4235K
DyLight 550	FAB4235L
DyLight 594	FAB4235M
DyLight 650	FAB4235W
DyLight 680	FAB4235Y
DyLight 755	FAB4235Z
FITC	FAB4235F
HRP	FAB4235H
Janelia Fluor 525	FAB4235JF525
Janelia Fluor 549	FAB4235I
Janelia Fluor 646	FAB4235J
Janelia Fluor 669	FAB4235JF669
PE	FAB4235P
PerCP	FAB4235C
mFluor Violet 450 SE	FAB4235MFV450
mFluor Violet 500 SE	FAB4235MFV500
mFluor Violet 610 SE	FAB4235MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4235-SP

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MAB6194

Integrin alpha 8 Antibody (481709) [Unconjugated]

(4 Publications)

100 μg

MAB6194

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="H4 human neuroglioma cell line was stained with Mouse Anti-Human Integrin a8 Monoclonal Antibody (Catalog # MAB6194, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Integrin a8 was detected in immersion fixed H4 human neuroglioma cell line using Mouse Anti-Human Integrin a8 Monoclonal Antibody (Catalog # MAB6194) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6194U-100UG
Alexa Fluor 405	FAB6194V-100UG
Alexa Fluor 488	FAB6194G-100UG
Alexa Fluor 532	FAB6194X
Alexa Fluor 594	FAB6194T-100UG
Alexa Fluor 647	FAB6194R-100UG
Alexa Fluor 700	FAB6194N-100UG
Alexa Fluor 750	FAB6194S-100UG
Alexa Fluor Plus 405	FAB6194AFP405-100UG
Alexa Fluor Plus 488	FAB6194AFP488-100UG
Alexa Fluor Plus 555	FAB6194AFP555-100UG
Alexa Fluor Plus 594	FAB6194AFP594-100UG
Alexa Fluor Plus 647	FAB6194AFP647-100UG
Alexa Fluor Plus 680	FAB6194AFP680-100UG
Allophycocyanin	FAB6194A
Allophycocyanin/Cy7	FAB6194APCCY7
Biotin	FAB6194B
CoraFluor 1	FAB6194CL1
DyLight 350	FAB6194D
DyLight 405	FAB6194E
DyLight 488	FAB6194K
DyLight 550	FAB6194L
DyLight 594	FAB6194M
DyLight 650	FAB6194W
DyLight 680	FAB6194Y
DyLight 755	FAB6194Z
FITC	FAB6194F
HRP	FAB6194H
Janelia Fluor 525	FAB6194JF525
Janelia Fluor 549	FAB6194I
Janelia Fluor 646	FAB6194J
Janelia Fluor 669	FAB6194JF669
PE/Atto594	FAB6194PEATT594
PE/Cy5.5	FAB6194PECY55
PE/Cy7	FAB6194PECY7
PerCP	FAB6194C
Phycoerythrin	FAB6194P
mFluor Violet 450 SE	FAB6194MFV450
mFluor Violet 500 SE	FAB6194MFV500
mFluor Violet 610 SE	FAB6194MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB6194P
Carrier Free	FAB6194A
Carrier Free	MAB6194-SP
Carrier Free	FAB6194V-100UG
Carrier Free	FAB6194U-100UG
Carrier Free	FAB6194T-100UG
Carrier Free	FAB6194S-100UG
Carrier Free	FAB6194R-100UG
Carrier Free	FAB6194N-100UG
Carrier Free	FAB6194G-100UG

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MAB3518

Integrin alpha 7 Antibody (334908) [Unconjugated]

(4 Publications)

100 μg

MAB3518

MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." alt="C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin a7 Monoclonal Antibody (Catalog # MAB3518, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3518U-100UG
Alexa Fluor 405	FAB3518V-100UG
Alexa Fluor 405	FAB3518V-025UG
Alexa Fluor 488	FAB3518G-100UG
Alexa Fluor 532	FAB3518X
Alexa Fluor 594	FAB3518T-100UG
Alexa Fluor 647	FAB3518R-100UG
Alexa Fluor 700	FAB3518N
Alexa Fluor 750	FAB3518S-100UG
Alexa Fluor Plus 405	FAB3518AFP405-100UG
Alexa Fluor Plus 488	FAB3518AFP488-100UG
Alexa Fluor Plus 555	FAB3518AFP555-100UG
Alexa Fluor Plus 594	FAB3518AFP594-100UG
Alexa Fluor Plus 647	FAB3518AFP647-100UG
Alexa Fluor Plus 680	FAB3518AFP680-100UG
Allophycocyanin	FAB3518A
Allophycocyanin/Cy7	FAB3518APCCY7
Biotin	FAB3518B
CoraFluor 1	FAB3518CL1
DyLight 350	FAB3518D
DyLight 405	FAB3518E
DyLight 488	FAB3518K
DyLight 550	FAB3518L
DyLight 594	FAB3518M
DyLight 650	FAB3518W
DyLight 680	FAB3518Y
DyLight 755	FAB3518Z
Fluorescein	FAB3518F
HRP	FAB3518H
Janelia Fluor 525	FAB3518JF525
Janelia Fluor 549	FAB3518I
Janelia Fluor 646	FAB3518J
Janelia Fluor 669	FAB3518JF669
PE/Atto594	FAB3518PEATT594
PE/Cy5.5	FAB3518PECY55
PE/Cy7	FAB3518PECY7
PerCP (Peridinin-chlorophyll Protein Complex)	FAB3518C
Phycoerythrin	FAB3518P
mFluor Violet 450 SE	FAB3518MFV450
mFluor Violet 500 SE	FAB3518MFV500
mFluor Violet 610 SE	FAB3518MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3518C
Carrier Free	FAB3518F
Carrier Free	FAB3518P
Carrier Free	FAB3518A
Carrier Free	FAB3518N
Carrier Free	MAB3518-SP
Carrier Free	FAB3518V-025UG
Carrier Free	FAB3518U-100UG
Carrier Free	FAB3518T-100UG
Carrier Free	FAB3518S-100UG
Carrier Free	FAB3518R-100UG
Carrier Free	FAB3518G-100UG
Carrier Free	FAB3518V-100UG

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H00003680-M01

Integrin alpha 9 Antibody (3E4) - Azide and BSA Free

(6 Publications)

0.1 mg

H00003680-M01

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-76482

Integrin alpha 8 Antibody (CL7304) - BSA Free

100 ul

NBP2-76482

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody (CL7304) [NBP2-76482] - Analysis in human lung and pancreas tissues. Corresponding ITGA8 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody (CL7304) [NBP2-76482] - Staining of human lung shows strong membranous positivity in pneumocytes.

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody (CL7304) [NBP2-76482] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44412AF350
Alexa Fluor 405	NBP3-44412AF405
Alexa Fluor 488	NBP3-44412AF488
Alexa Fluor 532	NBP3-44412AF532
Alexa Fluor 594	NBP3-44412AF594
Alexa Fluor 647	NBP3-44412AF647
Alexa Fluor 700	NBP3-44412AF700
Alexa Fluor 750	NBP3-44412AF750
Alexa Fluor Plus 405	NBP3-44412AFP405
Alexa Fluor Plus 488	NBP3-44412AFP488
Alexa Fluor Plus 555	NBP3-44412AFP555
Alexa Fluor Plus 594	NBP3-44412AFP594
Alexa Fluor Plus 647	NBP3-44412AFP647
Alexa Fluor Plus 680	NBP3-44412AFP680
Allophycocyanin	NBP3-44412APC
Biotin	NBP3-44412B
CoraFluor 1	NBP3-44412CL1
DyLight 350	NBP3-44412UV
DyLight 405	NBP3-44412V
DyLight 488	NBP3-44412G
DyLight 550	NBP3-44412R
DyLight 594	NBP3-44412DL594
DyLight 650	NBP3-44412C
DyLight 680	NBP3-44412FR
DyLight 755	NBP3-44412IR
FITC	NBP3-44412F
HRP	NBP3-44412H
Janelia Fluor 525	NBP3-44412JF525
Janelia Fluor 549	NBP3-44412JF549
Janelia Fluor 646	NBP3-44412JF646
Janelia Fluor 669	NBP3-44412JF669
PE	NBP3-44412PE
PerCP	NBP3-44412PCP
mFluor Violet 450 SE	NBP3-44412MFV450
mFluor Violet 500 SE	NBP3-44412MFV500
mFluor Violet 610 SE	NBP3-44412MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44412
BSA Free	NBP2-76482-25ul

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AF6498

Integrin alpha 11 Antibody [Unconjugated]

(2 Publications)

100 μg

AF6498

HAF016). A specific band was detected for Integrin a11 at approximately 140-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Integrin a11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Integrin a11 at approximately 140-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Integrin a11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin a11 at approximately 140-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Integrin alpha 11 was detected in immersion fixed irradiated mouse embryonic fibroblasts (iMEF) using Mouse Integrin alpha 11 Affinity-purified Polyclonal Antibody (Catalog # AF6498) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6498U-100UG
Alexa Fluor 405	AF6498V-100UG
Alexa Fluor 488	AF6498G-100UG
Alexa Fluor 532	AF6498X-100UG
Alexa Fluor 594	AF6498T-100UG
Alexa Fluor 647	AF6498R-100UG
Alexa Fluor 700	AF6498N-100UG
Alexa Fluor 750	AF6498S-100UG
Alexa Fluor Plus 405	AF6498AFP405-100UG
Alexa Fluor Plus 488	AF6498AFP488-100UG
Alexa Fluor Plus 555	AF6498AFP555-100UG
Alexa Fluor Plus 594	AF6498AFP594-100UG
Alexa Fluor Plus 647	AF6498AFP647-100UG
Alexa Fluor Plus 680	AF6498AFP680-100UG
Allophycocyanin	AF6498A
Biotin	AF6498B
CoraFluor 1	AF6498CL1
DyLight 350	AF6498D
DyLight 405	AF6498E
DyLight 488	AF6498K
DyLight 550	AF6498L
DyLight 594	AF6498M
DyLight 650	AF6498W
DyLight 680	AF6498Y
DyLight 755	AF6498Z
FITC	AF6498F
HRP	AF6498H
Janelia Fluor 525	AF6498JF525
Janelia Fluor 549	AF6498I
Janelia Fluor 646	AF6498J
Janelia Fluor 669	AF6498JF669
PE	AF6498P
PerCP	AF6498C
mFluor Violet 450 SE	AF6498MFV450
mFluor Violet 500 SE	AF6498MFV500
mFluor Violet 610 SE	AF6498MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6498-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Integrin alpha 9 Antibody [Unconjugated]

Integrin alpha 8 Antibody [Unconjugated]

Integrin alpha 7 Antibody - BSA Free

Integrin alpha 9 Antibody

Integrin alpha 8 Antibody - BSA Free

Integrin alpha 9 Antibody (560201)

Integrin alpha 11 Antibody (396214) [Unconjugated]

Integrin alpha 8 Antibody (481709) [Unconjugated]

Integrin alpha 7 Antibody (334908) [Unconjugated]

Integrin alpha 9 Antibody (3E4) - Azide and BSA Free

Integrin alpha 8 Antibody (CL7304) - BSA Free

Integrin alpha 11 Antibody [Unconjugated]