Integrin alpha 9 Antibody

Western Blot: Integrin alpha 9 Antibody [NBP2-16972] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 9 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Integrin alpha 9 Antibody [NBP2-16972] - Cultured human keratinocytes plated on fibronectin coated coverslips. Anti integrin alpha 9 antibody diluted 1:50 in 5% BSA, incubated at room temperature for 3 hours. Nuclei stained with DAPI (green) and f-actin with phalloidin (purple). ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Integrin alpha 9 Antibody [NBP2-16972] - Human hepatoma, using Integrin alpha 9 antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

• Reactivity: Hu, Mu, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Integrin alpha 9 Antibody Summary

• Description: Novus Biologicals Rabbit Integrin alpha 9 Antibody (NBP2-16972) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Integrin alpha 9 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Integrin alpha 9. The exact sequence is proprietary.
• Localization: Membrane, Single-pass type I membrane protein
• Predicted Species: Mouse (92%), Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITGA9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Validated from a verified customer review
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Western Blot 1:500 - 1:3000
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Integrin alpha 9 Antibody

• ALPHA-RLC
• Integrin alpha 9
• integrin alpha-9
• Integrin alpha-RLC
• integrin, alpha 9
• ITGA4L
• ITGA9
• RLC
• RLC-alpha

Background

This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane glycoproteins composed of an alpha chain and a beta chain that mediate cell-cell and cell-matrix adhesion. The protein encoded by this gene, when bound to the beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of this gene has been found to be upregulated in small cell lung cancers. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Integrin alpha 9 Antibody (NBP2-16972)(3)

Publications using NBP2-16972

• Muramatsu J, Hashimoto M, Miura S et al. Groove-aided sacrificial molding for fabrication of an in vitro vascular model with branches using ECM-derived materials. Lab on a Chip 2025-09-09 [PMID: 40765438]
• Vehlow A, Cordes N Growth factor receptor and beta-1 integrin signaling differentially regulate basal clonogenicity and radiation survival of fibroblasts via a modulation of cell cycling In vitro cellular & developmental biology. Animal 2022-02-22 [PMID: 35194763] (WB)
• Hight-Warburton W, Felix R, Burton A et al. ?4/?9 Integrins Coordinate Epithelial Cell Migration Through Local Suppression of MAP Kinase Signaling Pathways Frontiers in Cell and Developmental Biology 2021-11-25 [PMID: 34900996] (Western Blot, Block/Neutralize, Immunocytochemistry/ Immunofluorescence)

Review for Integrin alpha 9 Antibody (NBP2-16972) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 10/25/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured Human Keratinocytes,Paraformeldehyde Fixed Cells
• Species: Human
• Lot: 40688

Bioinformatics

• Gene Symbol: ITGA9
• Uniprot:
  • Q13797()