Integrin alpha 7 Antibody (334908) [Unconjugated]

C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin a7 Monoclonal Antibody (Catalog # MAB3518, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 334908
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 7 Antibody (334908) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 7
  Phe34-Pro1036
  Accession # NP_032424
• Specificity: Detects mouse Integrin alpha 7 in direct ELISAs. Reactivity with other Integrin alpha 7 isoforms was not tested. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 2, alpha 3, alpha 4, alpha 5, alpha 9, alpha E, alpha X, recombinant human Integrin alpha L, or alpha 11 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 7 Antibody (334908) [Unconjugated]

• FLJ25220
• integrin alpha 7 chain
• Integrin alpha 7
• integrin alpha-7
• integrin, alpha 7
• ITGA7

Background

Integrin alpha 7 is a transmembrane glycoprotein that forms heterodimers with Integrin  beta 1 to bind Laminin 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 7 Antibody (MAB3518)(4)

Publications using MAB3518

• Packer D, Martin PT. Micro-laminin gene therapy can function as an inhibitor of muscle disease in the dy(W) mouse model of MDC1A Mol Ther Methods Clin Dev 4/19/2021 [PMID: 33869655]
  
  Details:
  Citation using the FITC version of this antibody.
• M Farnoodian, CM Sorenson, N Sheibani PEDF Expression Affects the Oxidative and Inflammatory State of Choroidal Endothelial Cells Am. J. Physiol., Cell Physiol., 2018-01-10;0(0):. 2018-01-10 [PMID: 29351407] (Flow Cytometry, Mouse)
• Amanda L. York, James Q. Zheng Super-Resolution Microscopy Reveals a Nanoscale Organization of Acetylcholine Receptors for Trans-Synaptic Alignment at Neuromuscular Synapses eNeuro 2017-07-31 [PMID: 28798955]
• Yu M, Eckart MR, Morgan AA, Mukai K, Butte AJ, Tsai M, Galli SJ Identification of an IFN-gamma/mast cell axis in a mouse model of chronic asthma. J. Clin. Invest., 2011-08-01;121(8):3133-43. 2011-08-01 [PMID: 21737883] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: ITGA7
• Uniprot:
  • NP_032424()