Integrin alpha 9 Antibody (560201)

Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Integrin a9 Monoclonal Antibody (Catalog # MAB4574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Expression of VEGF-D-binding receptors by 468LN cells:(A) Expression of VEGF-R2 and R3 mRNA examined with semi quantitative RT-PCR, and (B) alpha 9 integrin mRNA measured with qRT-PCR. (C) Confirmation of VEGF-R2, VEGF-R3, and alpha 9 integrin expression at protein levels by western blot. MDA-MB-231 and MCF7-COX-2 cells were used as positive controls primarily for VEGF-R2 and VEGF-R3 expression. Differentially high alpha 9 integrin expression by 468 LN cells was evident. Bars represent mean (n = 4) ± SE, *,P<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0035094), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clone: 560201
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 9 Antibody (560201) Summary

• Immunogen: E. coli-derived recombinant human Integrin alpha 9
  Val89-Leu169
  Accession # Q13797
• Specificity: Detects human Integrin alpha 9 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin alpha 1, 2, 2b, 3, 4, 5, 6, 6 (x1), 8, 10, 11, D, E, L, M, V, X, recombinant mouse Integrin alpha 7 (x2) or 9 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 9 Antibody (560201)

• ALPHA-RLC
• Integrin alpha 9
• integrin alpha-9
• Integrin alpha-RLC
• integrin, alpha 9
• ITGA4L
• ITGA9
• RLC
• RLC-alpha

Background

Integrin alpha 9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 Integrin. alpha 9 beta 1 binds to VEGF-C and D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse Integrin  alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg 566‑Val 567). The ECD of mouse Integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human Integrin alpha 9, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 9 Antibody (MAB4574)(6)

Publications using MAB4574

• Chen, D;Wiggins, D;Sevick, EM;Davis, MJ;King, PD; An EPHB4-RASA1 signaling complex inhibits shear stress-induced Ras-MAPK activation in lymphatic endothelial cells to promote the development of lymphatic vessel valves bioRxiv : the preprint server for biology 2023-11-23 [PMID: 38045382] (Western Blot, Human)
• Osuka, K;Ohmichi, Y;Ohmichi, M;Honma, S;Suzuki, C;Aoyama, M;Iwami, K;Watanabe, Y;Miyachi, S; Angiogenesis in the Outer Membrane of Chronic Subdural Hematomas through Thrombin-Cleaved Osteopontin and the Integrin ?9 and Integrin ?1 Signaling Pathways Biomedicines 2023-05-13 [PMID: 37239111] (Western Blot, IHC, Human)
• Ogunsina O, Banerjee R, Knauer LA, Yang Y. Pharmacological inhibition of FOXO1 promotes lymphatic valve growth in a congenital lymphedema mouse model Frontiers in Cell and Developmental Biology 2023-01-05 [PMID: 36742198]
• Rivera-Serrano EE, Gonzalez-Lopez O, Das A, Lemon SM. Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses Elife 2/25/2019 [PMID: 30801249]
• D Choi, E Park, E Jung, B Cha, S Lee, J Yu, PM Kim, S Lee, YJ Hong, CJ Koh, CW Cho, Y Wu, NL Jeon, AK Wong, L Shin, SR Kumar, I Bermejo-Mo, RS Srinivasan, IT Cho, YK Hong Piezo1 incorporates mechanical force signals to genetic program that governs lymphatic valve development and maintenance JCI Insight, 2019-03-07;0(0):. 2019-03-07 [PMID: 30676326] (ICC, Western Blot, Human)
• Majumder M, Tutunea-Fatan E, Xin X, Rodriguez-Torres M, Torres-Garcia J, Wiebe R, Timoshenko AV, Bhattacharjee RN, Chambers AF, Lala PK Co-expression of alpha9beta1 integrin and VEGF-D confers lymphatic metastatic ability to a human breast cancer cell line MDA-MB-468LN. PLoS ONE, 2012-04-24;7(4):e35094. 2012-04-24 [PMID: 22545097] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ITGA9
• Uniprot:
  • Q13797()